Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4770CA45.8% 226.4 / 185.1 368intergenic (+255/‑420)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (118/75);  new base A (42/121);  total (160/208)
Fisher's exact test for biased strand distribution p-value = 2.09e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 421 total aligned reads displayed.

AAGTAGTGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCT  >  CP016816/202350‑202598
                                                                                                                               |                                                                                                                           
atgtatttaaGTTCGTTTAAATAATAGATTTAAGGTTAATTAAAATAACATAAACTTAATAATTCTTATAATGTAGATACTCGTGATATATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAc                                                                                                                             <  2:1986773/120‑1 (MQ=255)
aattatttaatttAGTGTAAATAATATATTTAATGTTAATTAAAATAACATAAAATTAATAATTCTTATAATTTAGATCCTCGTGATTTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAc                                                                                                                             <  2:1018696/117‑1 (MQ=255)
aagtaGTGAATGTAGTGTAAATAATAGAGTTTAGGTTATTTATAATACCATAAACTTAATAATTCTTATAATGTAGATGCTCGTGATTTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAc                                                                                                                             <  2:2891850/128‑1 (MQ=255)
aagtaGTGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAc                                                                                                                             <  1:169937/128‑1 (MQ=255)
 tctagtGTAGTTAGTGTAAATAATCGAGTTAAGGTTAATTAAAATCCCATAACCTTAATAATTCTTAAAATGTAGATCCGCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACa                                                                                                                            <  2:1613252/126‑1 (MQ=255)
 agtagtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACa                                                                                                                            <  1:3555047/128‑1 (MQ=255)
 agtagtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACa                                                                                                                            <  1:3679210/128‑1 (MQ=255)
  gtagtGTATTTTGTGTAAATTATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATATTGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACaa                                                                                                                           <  2:3745553/128‑1 (MQ=255)
  gtagtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACaa                                                                                                                           <  1:620287/128‑1 (MQ=255)
   tattGAAGTTAGTTTAAATAAAAGAGTTAAGGTTAATTAAAATACCATAAAATTAATAATTATTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAt                                                                                                                          <  1:2943544/125‑1 (MQ=255)
   tagtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAt                                                                                                                          <  2:60119/128‑1 (MQ=255)
   tagtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAt                                                                                                                          <  2:2258875/128‑1 (MQ=255)
   tagtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATAACATAAACTTAATAATTATTATAATGTAGATCCTCTTGATCTATTTTTATATATATTTATATTCACAAATAaaaaaaaaaaaaa                                                                                                                          >  1:3793067/1‑127 (MQ=255)
    agtGAATTTATTGTAAATAATAGATTTAATGTTAATTAAAATACAATAAACTTAATAATTCTTATAATGTAGATAATCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATc                                                                                                                         <  1:1834260/128‑1 (MQ=255)
    agtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTTATCTATTTTTTTATTTAATTATCTTCACAAATAAAAAAAAAAAAATc                                                                                                                         >  1:333745/1‑128 (MQ=255)
    agtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATc                                                                                                                         <  1:2289597/128‑1 (MQ=255)
    agtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTATTTATCTTCACAAATAaaaaaaaaaaaaaa                                                                                                                         >  2:2211540/1‑126 (MQ=255)
    agtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGAGATCTATTATTTTATTTATTTATCTTCACAAATAAAAAAAAAACAATc                                                                                                                         >  1:3153844/1‑128 (MQ=255)
    agtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATATAGATAATCATTATCTATTTATTTATTTATTTAAATAAAAAAATAaaaaaaaaaaaaaa                                                                                                                         >  2:685474/1‑126 (MQ=255)
    agtGAAGTTAGTGTAAATAATAGAGATAAGGTTAAATAAAATACCATAAACTTAATAATTCTTATAATCTATATCCACATGATCTATAAATTAATATATTTAAAAAAACAaaaaaaaaaaaaaaaaaa                                                                                                                         >  2:2122379/1‑126 (MQ=255)
     gtGAAGTTAGTGTAAATAATAGAGTTTAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCCATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATcc                                                                                                                        <  2:3299390/128‑1 (MQ=255)
     gtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATcc                                                                                                                        <  1:988723/128‑1 (MQ=255)
     gtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTAAAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATcc                                                                                                                        <  1:756832/128‑1 (MQ=255)
     gagattttAGTTTAAATAATAGAGTTAAGTTTAATTAAAATAACATAAACTTAATAATTATAATAATGTATATCATTGTGATCAATTTTTTTATTTGTTTATTTTCACAAATAAAAAAAAAACAATcc                                                                                                                        <  1:717610/122‑1 (MQ=255)
      tGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTTTTTATCTTCACAAATAaaaaaaaaaaaaataa                                                                                                                       >  1:865949/1‑124 (MQ=255)
       gAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATcccc                                                                                                                      >  2:476096/1‑128 (MQ=255)
        aaGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCt                                                                                                                     >  2:3254348/1‑128 (MQ=255)
         aGTTAGTGAAAATAATAGAGATAAGGTTAATTAAAATAACATAAACTTAATAATTCTTATAATGAAGATACTCTTGATCTATATTATTATTTTTTTATCTTCAAAAATAAAAAAAAAACAAtaaacta                                                                                                                    >  2:1431187/1‑122 (MQ=255)
          gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGATTATCTTCACAAAAAAAAAAAAAACAATCCCAtta                                                                                                                   >  1:3122846/1‑127 (MQ=255)
           ttAGTGTAAATAATAGAGTTAAGTTTAATTAAAATAACATAAACATAATAATTCTTATAATGTATATACTAGTTATCTATTATTTTAATTTTTTATATTAACAAAAAAAAAAAAAACAATcaacttaa                                                                                                                  >  2:3413936/1‑121 (MQ=255)
            tAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAAAccaataaaa                                                                                                                 >  2:1350260/1‑121 (MQ=255)
             attgtAAATAAAATAGTTAATTTTAATTAAAATAACATAAAATTAATAATTCTTATAATTTATAAACTCGTTATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCttaaaa                                                                                                                <  1:102321/126‑5 (MQ=255)
             aGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACACttaaaa                                                                                                                >  2:3628700/1‑124 (MQ=255)
              gtgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCACtaaaaaa                                                                                                               >  1:3852786/1‑122 (MQ=255)
              gtgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCATCTTGATCTATTTATTTATATTTTTATCATAACAAATAAAAAAAAAACaaaacaattaaaaa                                                                                                               >  1:2459295/1‑116 (MQ=255)
              gtgtAAATAATAGAGTTAAGGTTAATTAAAATAACATAAACTTAATAATTCTTATAATTTAGATCATCTTTATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAAtacaataaaaaa                                                                                                               >  1:3383482/1‑117 (MQ=255)
              gtgtAAATAATAGAGTTAAGGTTAATTAAAATAACATAAACTTAATAATTCTTATAATGTATATACTCTTGATATATTTTTTTATTTTTTTATATTAACAAATAaaaaaaaaaaaaacaaaataaaaa                                                                                                               >  1:1141505/1‑116 (MQ=255)
                   aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTAAAATGTAGATCCTCGTGATCTATTTTTTTATTTGATTATCTACACAAATAAAAAAAAAACAAAccccaaaaaaaataaa                                                                                                          >  2:2470416/1‑116 (MQ=255)
                     taataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATc                                                                                                                         <  1:1092261/111‑1 (MQ=255)
                     taataGAGTTAAGGTTAATTAAAATACAATAAAATTAATAATTCTTATAATTTAGATCCTCGTGATCTATATAATTATTATTTTATCTTCACAAAT‑AAAAAAAAACAATCACC‑‑‑TAAAA‑AATtaaaaaa                                                                                                   >  1:1203542/1‑128 (MQ=255)
                            tttAAGGTTAATTAAAAAAACAAAAAATTAAAAAATCTTAAAATTTATATCCTCGTGATCTATTTTTTTTTTTGTTTATATTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaa                                                                                             <  2:339258/127‑1 (MQ=255)
                              tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCC‑‑AAAAAAA‑AATaaaaaaaaa‑aaaaa                                                                                           >  2:1547589/1‑128 (MQ=255)
                               aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTAATTTATAATTTTATTATCTTCACAAATAAAAAAAAAACAATAAAAAT‑‑‑AAA‑AATaaaaaaaaa‑aaaaaaa                                                                                         >  2:2331839/1‑128 (MQ=255)
                               aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaa                                                                                          >  2:3080428/1‑128 (MQ=255)
                               aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAA‑ATTaaaaaaaaa‑aaaaaa                                                                                          >  2:3022784/1‑128 (MQ=255)
                               aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTATTATATTTCTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑‑TAAAA‑AATtaaaaaaaa‑aaaaaaa                                                                                         >  2:203155/1‑128 (MQ=255)
                               aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCATGATCTATTTATTTATATATTAAAATTAACAAATAAAAAAAAAACAAT‑‑ACAAATAAAA‑ATTaaaaaaaaa‑aaaaaa                                                                                          >  1:3081405/1‑128 (MQ=255)
                                atgTTTATTAAAATAAAATAAAATTAATAATTTTTATAATTTTTTTACTTGTTTTTTATTTTTTTTTTTTTTTATTTTAAAAAATAAAAAAAAAAAAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaa                                                                                         <  2:2997617/126‑1 (MQ=255)
                                aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATATTTTTATTTTTTTATAATCACAAATAAAAAAAAAAAAA‑‑ACAAATTAAAA‑ATTaaaaaaaaa‑aaaaaaa                                                                                         >  2:2572302/1‑128 (MQ=255)
                                 ttttaataatAATACAATAAAATTAATATATATAATAATTTTTATCATCGGTTTTTTTTTTTTTTTTTTTATATATTAAAAAATAAAAAAAAAACAATCCCC‑‑TTAAAA‑AATaaaaaaaaa‑aaaaaaaa                                                                                        <  1:3250595/126‑1 (MQ=255)
                                 ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaa                                                                                        >  2:3179824/1‑128 (MQ=255)
                                 ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATATATATAATCGTGATCTATTTTATTATTTATATATATTAACAAATAAAAAAAAAAAAAAAAC‑‑AATAAAA‑AATaaaaaaaaa‑aaaaaaaa                                                                                        >  2:2673967/1‑128 (MQ=255)
                                  gTTAATTAAAATAACATAAAATTAAAAATTAATAAAATTTTGATACTTTTTTTATATTTTTTTAATTTTTTATATTAACAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaaa                                                                                       <  1:1287151/128‑1 (MQ=255)
                                   ttaattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACAC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaaaa                                                                                      >  1:2930303/1‑128 (MQ=255)
                                   ataattaaAAAAACATAAAAATAAAAAATATTATAATTTATATCCTTGTTTTTTTTTTTTTTTTTTTTTTATTATAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaaaa                                                                                      <  2:1063531/127‑1 (MQ=255)
                                    taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATATTTTTATTTGTATATCTTCAAAAATAAAAAAAAAACAATCCAATTAaaaaa‑aaaaaaaaaaaa‑aaaaaaaaa                                                                                       >  2:2441219/1‑128 (MQ=255)
                                      attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAACAAACAC‑‑AAAAAAA‑ATTaaaaaaaaa‑aaaaaaaaaaaaa                                                                                   >  2:1761560/1‑127 (MQ=255)
                                      attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaaaaaaa                                                                                   >  2:2943664/1‑127 (MQ=255)
                                      attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCC‑‑TTAAAA‑ATTaaaaaaaaa‑aaaaaaaaaaaaa                                                                                   >  1:3059642/1‑127 (MQ=255)
                                      attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAAAACC‑‑ATTAAAA‑ATTaaaaaaaaa‑aaaaaaaaaaaaa                                                                                   >  2:1180227/1‑127 (MQ=255)
                                          aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTAAAAAT‑aaaaaaaaaaaa‑aaaaaaaaaaaaaac                                                                                 >  2:890151/1‑125 (MQ=255)
                                           aataaaataaaaataaaaATTTTTATAATTTATAAACTCTTTTTTTTTTTTTTTTTTTTTTTATATTAAAAAAAAAAAAAAAAAAAATACCC‑TTAAAAA‑TTAAAAAAAAAA‑AAAAAAAAAAAACTaaa                                                                               <  2:3523355/122‑1 (MQ=255)
                                           aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTGTTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTAaaaaaaaa‑aaaaaaaaaaaaaaaaaa                                                                              >  2:441587/1‑128 (MQ=255)
                                           aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATcc                                                                                                                        <  2:1680103/90‑1 (MQ=255)
                                           aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTAaaaaaaaa‑aaaaaaaaaaaaaaaaaa                                                                              >  2:3444236/1‑128 (MQ=255)
                                           aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTAaaaaaaaa‑aaaaaaaaaaaaaaaaaa                                                                              >  1:3006111/1‑128 (MQ=255)
                                                    aaCTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACaaaaaaacaaataa                                                                     >  1:2057100/1‑120 (MQ=255)
                                                     aattAAAAATTTTTATAATTGACAACCTCGGGTTCTATTTTTTTTTTTGTTTATATTCACAAAAAAAAAAAAAACAAACCCC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTa                                                                    <  1:3295516/126‑1 (MQ=255)
                                                      attAATAATTTAAATAATTTATAAACTCTGGATAAATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATACCCTTAAAAAT‑TAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAActct                                                                     <  1:3743939/127‑1 (MQ=255)
                                                             aatCTTATAATGTAGATCCTCGTGATCTATTATTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAA‑‑ACCAAAAAAAA‑ATTAAAAAAAAATAAAAAAAAAAAAAAAAAAACAAACTAAAtttataa                                                             >  2:919304/3‑124 (MQ=255)
                                                                        ttAGATACTCGTTTTTTTTTTTTTTTTTTTTTTTTATT‑AAAAAAAAAAAAAAAAAAAACACC‑TTAAAAT‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt                                                 <  2:773712/127‑1 (MQ=255)
                                                                          aGATCCTAGTTTTATATTTTTTTTTTTTTTTTTATTAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                               <  1:3619315/128‑1 (MQ=255)
                                                                            ataatatttttttatttttttattttttTATATTAAAAAATAAAAAAAAAACAATTCAC‑‑TTAAAA‑AAT‑AAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                            <  1:518682/116‑1 (MQ=255)
                                                                             tctacttgtgatatttttttattttttTATATTAAAAAAAAAAAAAAAAAAAATACCC‑‑TTAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                            <  1:892477/117‑1 (MQ=255)
                                                                              aaatatttattATTTTTTTAATTTTTTAAAAAAAAAAAAAAAAAAAAAAAAATACC‑‑ATAAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                           <  1:1590884/118‑1 (MQ=255)
                                                                               aaagtttattatttttttattttttTATATTTAAAAATAAAAAAAAAAAAATCGAC‑TTTAATA‑TTTAAAAAAAAA‑AAAAAAATAAAAATAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                           <  1:2175697/119‑1 (MQ=255)
                                                                                ttgttttattttttttttttttttTATTTTAAAAAAAAAAAAAAAAAAAATAACATTAAAAAATAAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                            <  1:212113/118‑1 (MQ=255)
                                                                                  gttttatatttttttattttttAAAATTAAAAAATAAAAAAAAAAAAAACCCATTAAAAAA‑TAAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcaca                                         <  1:2373765/122‑1 (MQ=255)
                                                                                   ttatttatttttttattttttTATATTTAAAAATAAAAAAAAAA‑AATACCC‑TTAAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                      <  1:2014184/123‑1 (MQ=255)
                                                                                   tgttCTATTTTTTTTTTTTTTTTTATTTAAAAAAAAAAAAAAAAAAAATCCC‑‑ATAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                      <  2:3674263/125‑1 (MQ=255)
                                                                                    tatatatttttttattttttTATCATTAAAAATAAAAAAAAAACAATTCCC‑‑TTAAAA‑TTTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                     <  1:3442654/124‑1 (MQ=255)
                                                                                        tttttttttatttttttttattTAAAAATAAAAAAAAAAAAATACAC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttattt                                 <  1:3541415/126‑1 (MQ=255)
                                                                                        aatttttttatttttttATATTAAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAA‑AATTAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                <  1:1372611/127‑1 (MQ=255)
                                                                                         tattttttttttttttATAATTAAAAAAAAAAAAAAAAAAAATACCTTT‑‑AAA‑AAT‑AAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                               <  1:3521207/126‑1 (MQ=255)
                                                                                          tttttttAATTTTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                               <  1:2866647/128‑1 (MQ=255)
                                                                                            tttttattt‑tttatAATAACAAAAAAAAAAAAAAAAAAAACC‑ATAAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                             <  1:462045/128‑1 (MQ=255)
                                                                                             ttttttttttttATTTATAAAAAAAAAAAAAAAAAAAAACCC‑AAAAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                             <  1:2587726/128‑1 (MQ=255)
                                                                                             ttttATTTTTTTATAATAAAAAAAAAAAAAAAAAAAAATACC‑CTTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                             <  1:152230/128‑1 (MQ=255)
                                                                                              tttATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAA‑AATTAAAAAAAA‑AAAAAAAAAA‑‑‑TAAAAAAAAAATATATTAATTAAAATTACATATAATACCAAATTTTATTTTTAaaaaaat                       >  1:3175061/1‑127 (MQ=255)
                                                                                              tttAATTTTTTATTTTAAAAAATAAAAAAAAAAAAAACACC‑‑TTAAAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaaa                           <  2:3861378/128‑1 (MQ=255)
                                                                                                      tttATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAA‑AATTAAAAAAAA‑AAAAAAAAAAAA‑‑AAAACCAAAATATATTTATTAAAATTACATATTAAACCAAATATAATTTTTAAAAAAAGATAttta                >  2:3150396/1‑126 (MQ=255)
                                                                                                      ttaatataaaaaaaaaaaaaaaaaaaaATACCC‑TTAAAAA‑ATAAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                    <  1:3219155/117‑1 (MQ=255)
                                                                                                       ttatactCACAAATAAATAAAAAAAAAT‑‑CTAATTAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTATATTTATTATAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                  <  2:3603024/122‑1 (MQ=255)
                                                                                                          ttaattaaaaaaaaaaaaaaaaaaaACCC‑‑TTAAAA‑AAT‑AAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa              <  1:1350260/121‑1 (MQ=255)
                                                                                                          tataaaaaataaaaaaaaaaaaaATCCCC‑‑‑TAAAA‑AATTAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa              <  2:3793067/117‑1 (MQ=255)
                                                                                                           cttaaaaaaaaaaaaaaaaaaaaaACCCA‑‑TAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa              <  1:572290/122‑1 (MQ=255)
                                                                                                            ttaaaaaaaaaaaaaaaaaaaATAACC‑ATAAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa              <  2:3552557/123‑1 (MQ=255)
                                                                                                            ataaaaaaaaaaaaaaaaaaaATCACATT‑‑‑AAA‑AATTAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata            <  1:1976284/123‑1 (MQ=255)
                                                                                                            aataaaaaaaaaaaaaaaaaaaaaaCCATAT‑AAA‑ATTAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa              <  1:2751028/123‑1 (MQ=255)
                                                                                                                      aaaaaaaaaaaaACCCC‑‑TTAAAA‑ATTTAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATc   <  2:3356879/128‑1 (MQ=255)
                                                                                                                               |                                                                                                                           
AAGTAGTGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAA‑AATAAAAAAAAA‑AAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCT  >  CP016816/202350‑202598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑