Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA98.6% 7361.3 / 65.2 2420intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (1521/856);  minor base . (12/21);  total (1534/886)
Fisher's exact test for biased strand distribution p-value = 1.62e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2484 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349
                                                                                                                              |                                                                                                                                 
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                                >  1:3624042/1‑128 (MQ=255)
  atAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatt                                                                                                                               >  2:5782500/2‑128 (MQ=255)
   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                              >  2:2773005/1‑128 (MQ=255)
    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                             >  1:1228569/1‑128 (MQ=255)
     aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGAAAATTTTTAAAAaattaat                                                                                                                            >  1:5888763/1‑128 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                          >  1:2445824/1‑128 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                          <  2:2650408/128‑1 (MQ=255)
       agTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                          <  1:5552154/127‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                             <  1:2929715/124‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                         <  2:2992370/128‑1 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                       >  2:4690673/1‑128 (MQ=255)
             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                                    <  1:1539481/128‑1 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                  >  1:3794860/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                  >  1:3191559/1‑128 (MQ=255)
                ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTAACTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                 >  1:5823240/1‑128 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                                <  2:2910624/128‑1 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                                <  2:690307/128‑1 (MQ=255)
                     aGTTGATAATATTTAGTTTAATTAATTTATCGCATTTGTATGAATTAGTAAGAAAATAAAACTATTATCTTTTTAGATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTaaaaaa                                                                                                            <  1:5986814/128‑1 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGc                                                                                                       >  2:1470689/1‑128 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAga                                                                                                       >  2:3247674/1‑127 (MQ=255)
                           taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCtt                                                                                                    >  2:165630/1‑128 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                   >  1:5128758/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                           >  1:441103/1‑128 (MQ=255)
                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttctttt                                                                                          >  2:6561270/1‑128 (MQ=255)
                                      ttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAttttttttttttt                                                                                         >  1:1000505/1‑128 (MQ=255)
                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttttt                                                                                        >  1:2252971/1‑128 (MQ=255)
                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                        >  2:2405210/1‑128 (MQ=255)
                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                       >  2:2092999/1‑128 (MQ=255)
                                        aaTTAATTTATCGCACTTTTATGAAATTTTAAAAAACTAAAACTATTATCTTTTTATATATTTTTTGTATATGCAAAATTTTAAAAAATTAATTTGAACATGTAAAAAATAGCttttttttttttttt                                                                                       <  1:724597/127‑1 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                      >  1:6148635/1‑128 (MQ=255)
                                         aTTAATTTATAGCAATTGTTTTAATTATTAACAAACAAAAAATTTTTTGTTTTTATATATTTTTTGTATATTCAAATTTTTTAAAAATTAATTTTAACTTTTAAAAAAAAGCtttttttttttttttt                                                                                      <  2:5922509/128‑1 (MQ=255)
                                            aaTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttt                                                                                   >  2:3675395/1‑128 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGCtttttatttttattttttttt                                                                                 >  2:2187844/1‑126 (MQ=255)
                                                tttagCACTTTTATTAAATAGTAAGAAAATAAAAATATTATTTTTTTATTTATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAAAAGCTTTTTTTTTTTTTTTTtataatg                                                                               <  1:4761930/124‑4 (MQ=255)
                                                  tcgtagttGTATTAATTAGTAATAAAATAAAAAAATTATTATTTTATATATTTTTTTTTTTTTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATgg                                                                            <  1:3064937/122‑1 (MQ=255)
                                                   ctccctttttttaaTTAGTAACAAAAAAAAAAAATTTATTTTTTTTATATTTTTTTTAAATTCAAATTTTTTAAAAAAAAAATTTAACATGTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGa                                                                           <  1:2155588/116‑1 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCTAATTTTTAAAAAATTAATTAGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAAttaa                                                                           >  2:5170231/1‑125 (MQ=255)
                                                    gCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataaagaaat                                                                           >  2:1056805/1‑121 (MQ=255)
                                                          gTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGAAAATaaaa                                                                    >  2:6408610/1‑128 (MQ=255)
                                                          gTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAattaaa                                                                    >  1:4532356/1‑124 (MQ=255)
                                                          gTATGAAATAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAATTAGCTTTTTTTTTTTTTTTTTTTATTTGTAAATATaa                                                                    >  2:3806995/1‑128 (MQ=255)
                                                             tttattattAATAAACAAAAATTTATATATTTTTTTATATTTTTTGTTTATTCAAATTTTTAAAAAAATAATTTTCACTTGTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:1807719/125‑1 (MQ=255)
                                                             ttattatttattaaattaaaatttTTATTTTTTTTTTATTTTTTTTTTTTTGAAATTTTTTAAAAATTTATTTTTATTTTTTAAAAATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtttt                                                               <  2:5528170/116‑1 (MQ=255)
                                                             ataaTTATTAATTAAATAAAAATATTATTTTTTTATTTATTTTTTTTAATTTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:3085145/126‑1 (MQ=255)
                                                              gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATGTAAATATAAAttta                                                               >  1:501999/1‑127 (MQ=255)
                                                               tattattAATAAAAAAAAAATATTTTTTTTTTATTTATTTTTTTTTTTTTTAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:5041658/127‑1 (MQ=255)
                                                               aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGATAATATAATttttt                                                              >  2:4293356/1‑127 (MQ=255)
                                                                aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAAATAAAATTTaa                                                             >  2:817723/1‑127 (MQ=255)
                                                                 ttattaATAAAAAAAAAAAATTTTATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAAATAATTTTAACTTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                             <  2:5271763/128‑1 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTatt                                                            >  1:2559114/1‑126 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTGTAAATATAAAtttttgttt                                                          >  1:5658974/1‑123 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTATAATTTAAAAAAAAATTTTaaa                                                            >  1:4664978/1‑126 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATTTATATATAAAtttttttt                                                           >  1:1908139/1‑124 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAgtt                                                           >  2:124451/1‑126 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAAAGTAAATATAAAttttttgt                                                           >  2:4534685/1‑124 (MQ=255)
                                                                      aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCTTATTTTTTTTTTTTTTTTAAATTTTAATAATAAAtttaatttat                                                         >  1:5405737/1‑121 (MQ=255)
                                                                      aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAATAAGATTTTTTTTTTTTTTTTTTATAATGTAAAATTAAAATTtatttatt                                                        >  1:3486766/1‑128 (MQ=255)
                                                                       aGAAACTAAAAATTTTTTTTTTTTATTTTTTTTTTTTTTATTTTAATTTTTAAAAAATTTATTTTTACTTTTAAAATATATGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                       <  1:4066616/128‑1 (MQ=255)
                                                                        taaaataaaagttTTTTTTTTTTTGTTATTTTTTTTATATTTAAATTTTTAAAAAAATAAATTGTACTTTTTAAAAAAAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtttt                                                      <  1:3339086/127‑1 (MQ=255)
                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTattaatttttt                                                    >  1:4273099/1‑118 (MQ=255)
                                                                          aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTAtttttt                                                    >  2:4007624/1‑126 (MQ=255)
                                                                           actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTAATTAtttttt                                                    >  1:6473558/1‑126 (MQ=255)
                                                                               aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAATAAAAATTTTATTTATttttttttata                                              >  1:1464590/1‑123 (MQ=255)
                                                                                aacttTTATTTTTTTATATTTTTTTTTTATTTTCTAATTTTTAAAAAATTAATTTTAACTTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                             <  1:6357217/128‑1 (MQ=255)
                                                                                aaaatatatTTTTTTATTTATTTTTTTTTTATTTAAATTTTTAAAAAATTAAATTTTAATTTTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                              <  2:1379237/122‑1 (MQ=255)
                                                                                 actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATAAAATTTTTATGTATTTTGTTTTCTaa                                             >  2:5343849/1‑128 (MQ=255)
                                                                                 aatattatTTTTTTATATATTTTTTTTATAATTAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                             <  1:520624/126‑1 (MQ=255)
                                                                                  ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGAAAAAAAAAATTTTTTGTATTTTTTTTTATaat                                            >  2:229987/1‑127 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTAAAATAAAAATTTAATTTATttttttttaaaat                                            >  1:1657543/1‑121 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTAATATAATTTAAATAAAAATTTTAGTTATTTTTTTTTATAATTAt                                         >  1:2036089/1‑128 (MQ=255)
                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAATTATAAATTTTAGTTATTTTTTTTTATAATTAta                                        >  1:5738782/1‑127 (MQ=255)
                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTATGTATtttttttaataattatat                                       >  1:6062233/1‑116 (MQ=255)
                                                                                             ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTTTTTTTTTTTATAATTATATaaaaaaa                                >  1:6600925/1‑128 (MQ=255)
                                                                                              tatatatTTTTTTTATTTTTAAATTTTTAAAAAATTAATTTTTAATTGTAAAAAATATATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                                <  2:6149655/125‑1 (MQ=255)
                                                                                                ttttttttttttttttGTAATTTTTTTAAAAATTTATTTGTAATTGTTAAAAATTTGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                             <  1:391618/124‑1 (MQ=255)
                                                                                                 attttttttttATATTAAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                             <  2:5423104/125‑1 (MQ=255)
                                                                                                 atatTTTTTGTATATTAAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATATATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                             <  1:5473564/128‑1 (MQ=255)
                                                                                                 ataaTTTTTTTATATTTTAATTTTTTAAAAAATTTTTTTTTATTTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                             <  2:3577330/128‑1 (MQ=255)
                                                                                                  ttttttttGTATTTTAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATTTATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                           <  1:4901122/126‑1 (MQ=255)
                                                                                                  atttttttttATTTTAATATTTTTAAAAAATTAATTTTAAATATTTAAAAATTTTTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                             <  2:2548442/126‑1 (MQ=255)
                                                                                                   atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTATAATTATCTaaaaaaaaataa                           >  1:1361015/1‑123 (MQ=255)
                                                                                                    ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATATaa                           >  1:2678938/1‑128 (MQ=255)
                                                                                                     ttttttttTATGTAATTTTTAAAAAAATAATTTTAACATTTATAAAATAG‑‑GTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                      <  1:5321577/128‑1 (MQ=255)
                                                                                                     tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATTTTTTAAATATAAATTTTAATTAATATGTTTAATAAGTATATAAAAAAATAaaaaa                         >  2:4403622/1‑127 (MQ=255)
                                                                                                       tttttttttgaaaaTTTTTTAAAAATTAATTTTAACTTGTAAAAAATTTCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                       <  2:5619803/117‑1 (MQ=255)
                                                                                                       tttGTTTATTCAAATTTTTTAAAAAATAATTTTTACTTGTTAAAAATA‑‑TTATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                      <  1:4185109/128‑1 (MQ=255)
                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATTTAATTAAAAATTTTTGTTTTTTTTTTTTATATTATAAAAAAAAATaataaataa                      >  2:1567402/1‑120 (MQ=255)
                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTATTATTTTTTTTTTATAATTATATAAAAAAATAaaaatta                       >  1:919814/1‑125 (MQ=255)
                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTTTCTAATTATATAAAAAAATATAAAGTaa                      >  1:2359956/1‑128 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAATTATAAATTTTATTTATTTTTTTTTATAATTTTATAAAAAAATATAAATTaaaa                    >  1:1093248/1‑128 (MQ=255)
                                                                                                          ttttatttaaatttttAAAAAAATTATTTTTAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                    <  1:6233009/116‑1 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGGTATTTTGTTGTATAATTATATAAAAAAATATAAATGAAAAAaaat               >  1:1771656/1‑128 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTAACTTATTTTTTGGAATAATTAAATAAAAAAAAAGAAATAAAAAAatat               >  2:4173522/1‑128 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAAGGAAATATAAATTTTTGGTTTTTTGTTGTATAAGTAAAAAAATAAATAGAACGAAAAAataatt             >  2:6269527/1‑125 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTAGTTATTTTTTTTTATAATAAAATAAAAAAATAAAAAGGAAAAAATAtt              >  2:4923598/1‑128 (MQ=255)
                                                                                                                 cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTGTTTTCAAATTATATAAAAAAATAGAAAGGAAAAAATATTg             >  2:6033553/1‑128 (MQ=255)
                                                                                                                   aatttttTAAAAAATTAATTTTAACTATTAAAAAATATCTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATAtt              <  1:817796/125‑1 (MQ=255)
                                                                                                                      ttttTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTTTATAATTTTATAAAAAAATATAAATGAAAAAATATTTTTaa         >  1:2798952/1‑128 (MQ=255)
                                                                                                                         ttaaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATTAAAAATAAAAATTTATTTATTTTTTTTTAAAATTATAAAAAAAAATAAAAATTAAAAAATATTTTTaaa        >  2:1955497/3‑128 (MQ=255)
                                                                                                                         ttaaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAT‑‑TGTAAATATAAATTTTAGGTTTTTTGTTTTGTAAGTATATAAAAAAATAGAATGGAAAAAATATTTTTataatt     >  2:3428995/3‑126 (MQ=255)
                                                                                                                              |                                                                                                                                 
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: