Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 121,230 C→T G207S (GGT→AGT)  JCVSYN2_00465 ← spermidine/putrescine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816121,2300CT99.8% 8608.7 / NA 2773G207S (GGT→AGT) JCVSYN2_00465spermidine/putrescine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (1366/1393);  minor base A (7/0);  total (1380/1393)
Fisher's exact test for biased strand distribution p-value = 7.37e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2822 total aligned reads displayed.

ATTAATAGCATTTCAAGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACCACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACTTGATGATTTTTAGCT  >  CP016816/121105‑121354
                                                                                                                             |                                                                                                                            
    aTAGCATTTCAAGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGt                                                                                                                        <  1:6365614/128‑1 (MQ=255)
     tAGCATTTCAAGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGACACATTAGTTTGCATACTACCAGTa                                                                                                                       >  2:4645887/1‑128 (MQ=255)
      aGCATTTCAAGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATATATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTaa                                                                                                                      <  2:6022471/128‑1 (MQ=255)
      aGCATTTCAAGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTaa                                                                                                                      <  1:4316036/128‑1 (MQ=255)
          tttCAAGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATa                                                                                                                  >  2:642063/1‑128 (MQ=255)
           ttCAAGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATaa                                                                                                                 >  2:1894319/1‑128 (MQ=255)
           ttCAAGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTAACAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATaa                                                                                                                 <  1:3318886/128‑1 (MQ=255)
             cAAGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTCCCAGTAAAATAAGa                                                                                                               >  2:3165586/1‑128 (MQ=255)
              taGTAATAATAGATAAAGCAATTACTAAAACTTAACAAGTACCATAATAAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGaa                                                                                                              <  1:5309383/127‑1 (MQ=255)
              aaGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGaa                                                                                                              >  2:4555872/1‑128 (MQ=255)
              aaGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGaa                                                                                                              <  1:1241165/128‑1 (MQ=255)
                  aataatAGATAAAGCAATTACTAAAACTAAACATGTATCAAAAACAAAATTAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAaataa                                                                                                          <  2:144224/128‑1 (MQ=255)
                  aataatAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAaataa                                                                                                          <  1:6546452/128‑1 (MQ=255)
                   ataataGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAaataat                                                                                                         >  1:3004231/1‑128 (MQ=255)
                    taataGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAataata                                                                                                        >  1:5898143/1‑128 (MQ=255)
                     aataGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACGACCAGTAAAATAAGAAAtaataa                                                                                                       >  2:4904129/1‑128 (MQ=255)
                     aataGAAAAAGAAATTAATAAAACTAACAAAGTAACAAAAACAAAAATAAATGGTATATTTTTTTTATCAGTATAAATAAATGTTGAAACATTATTGTGCATAATAACATTAAAATAAGAAAtaataa                                                                                                       <  2:5689212/128‑1 (MQ=255)
                      ataGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATaaa                                                                                                      >  2:1171242/1‑128 (MQ=255)
                       taGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATaaaa                                                                                                     <  1:786692/128‑1 (MQ=255)
                         gATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAatc                                                                                                   <  1:3083044/128‑1 (MQ=255)
                             aaGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATCCTACCAGTAAAATAAGAAATAATAAAAtcttca                                                                                               >  1:1000298/1‑128 (MQ=255)
                             aaGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAAtcatca                                                                                               <  1:2325955/128‑1 (MQ=255)
                               gCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCaaa                                                                                             >  2:2805819/1‑128 (MQ=255)
                               gCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCaaa                                                                                             <  2:3046664/128‑1 (MQ=255)
                                cAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCaaaa                                                                                            <  1:366174/128‑1 (MQ=255)
                                 aaTTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATAGTACCAGTAAAATAAGAAATAATAAAatc                                                                                                   >  2:1311261/1‑120 (MQ=255)
                                  aTTACTAAAACTAAACAAGTACCATAAACAAAAATTAATGGTCTAGTTATTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTCGCATTAAAATAAGAAATAAAAAAAACATCAACACt                                                                                          >  2:5294635/1‑128 (MQ=255)
                                  aTTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACt                                                                                          <  2:2456889/128‑1 (MQ=255)
                                  aTTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACt                                                                                          <  2:789743/128‑1 (MQ=255)
                                    tACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCaa                                                                                              <  2:1012687/122‑1 (MQ=255)
                                    tACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCa                                                                                        <  1:915777/128‑1 (MQ=255)
                                     actaaaactaaaCAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCAt                                                                                       <  2:1777759/128‑1 (MQ=255)
                                          aactaaaCAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCaa                                                                                  >  2:6288571/1‑128 (MQ=255)
                                           actaaaCAAGTACCAATAACAAAAATAAATGGGCTAGATTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCaaa                                                                                 <  1:4782010/128‑1 (MQ=255)
                                           actaaaCAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCaaa                                                                                 <  1:1885530/128‑1 (MQ=255)
                                           actaaaCAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCaaa                                                                                 <  1:5761296/128‑1 (MQ=255)
                                            ctaaaCAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCaaaa                                                                                <  1:2426750/128‑1 (MQ=255)
                                               aaCAAGTACCAAATACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCa                                                                             <  2:3794857/128‑1 (MQ=255)
                                               aaCAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGGAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCa                                                                             >  1:3548144/1‑128 (MQ=255)
                                               aaCAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTATAATTAGAAATAATATAATCATAATAACTCATTGCAAAATCa                                                                             >  2:4176399/1‑128 (MQ=255)
                                                aCAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCaa                                                                            >  2:5569905/1‑128 (MQ=255)
                                                  aaGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATa                                                                          <  1:5002345/128‑1 (MQ=255)
                                                    gTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTc                                                                                         >  1:2222691/1‑111 (MQ=255)
                                                     tACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATagca                                                                       >  2:35180/1‑128 (MQ=255)
                                                      aCCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGAAAAATAAGAAATAATAAAATAAACAAAACTCAATGCAAAAGCAATagcag                                                                      >  1:2921609/1‑128 (MQ=255)
                                                       ccAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCATTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAATCTCATTGCAAAAGCAATacgagc                                                                     >  2:2934861/1‑123 (MQ=255)
                                                       ccAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATagcagc                                                                     >  1:2124977/1‑128 (MQ=255)
                                                         aaaaacaaaaaTAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTg                                                                   <  2:1543876/128‑1 (MQ=255)
                                                           aaacaaaaaTAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTTCCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCa                                                                 >  1:6002889/1‑128 (MQ=255)
                                                           aaacaaaaaTAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCa                                                                 >  1:137439/1‑128 (MQ=255)
                                                            aacaaaaaTAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAg                                                                >  1:553509/1‑128 (MQ=255)
                                                             acaaaaaTAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAATAACTAATAAAATCATCAAATCTCCTTGCATCAGCAATAGCAGCTGCAGt                                                               >  2:5924994/1‑128 (MQ=255)
                                                              caataaTAAATTGTATAGTTTATATAGAAGTATAAATAAATGTTGAAACATTAGTTAGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGta                                                              <  2:2022024/128‑1 (MQ=255)
                                                               aaaaaTAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCaaa                                                                                 <  1:6632736/108‑1 (MQ=255)
                                                                 aaataaatGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGtaata                                                           >  1:1626630/1‑128 (MQ=255)
                                                                 aaataaatGGTCTAGTTTTTTTAGCAGTATAAATAAATGATGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGtaata                                                           <  1:1137993/128‑1 (MQ=255)
                                                                  aataaatGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGtaataa                                                          <  1:5125645/128‑1 (MQ=255)
                                                                   ataaatGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTaataat                                                         <  2:2140550/128‑1 (MQ=255)
                                                                   ataaatGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTaataat                                                         <  1:3232570/128‑1 (MQ=255)
                                                                    taaatTGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAAtt                                                        <  2:256116/128‑1 (MQ=255)
                                                                     aaatGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTg                                                       >  1:1482800/1‑128 (MQ=255)
                                                                     aaatGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTg                                                       <  1:1750372/128‑1 (MQ=255)
                                                                       atGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGc                                                                  <  1:2684931/115‑1 (MQ=255)
                                                                       atGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCt                                                     >  1:6211745/1‑128 (MQ=255)
                                                                        tGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTg                                                    >  1:5431095/1‑128 (MQ=255)
                                                                          gTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATTAAATCATCAAACCTCATTGCTAAAGCAATAGCAGCTGCAGTAAGAAGTGCTggg                                                  >  2:6136580/1‑127 (MQ=255)
                                                                          gTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGt                                                  >  2:696218/1‑128 (MQ=255)
                                                                           tCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCaa                                                                                  >  1:3418890/1‑95 (MQ=255)
                                                                             tAGTTTTTTTAGTAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGtttt                                               >  1:4868011/1‑128 (MQ=255)
                                                                              aGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTa                                              >  2:6560904/1‑128 (MQ=255)
                                                                                   ttttAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTATAATAAGAATTAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATa                                         >  2:920964/1‑128 (MQ=255)
                                                                                    tttAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAg                                        <  2:3280506/128‑1 (MQ=255)
                                                                                      tAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAAAAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGt                                      <  2:4287958/128‑1 (MQ=255)
                                                                                       aGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTa                                     <  1:4454475/128‑1 (MQ=255)
                                                                                          aGTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAata                                  <  1:6095913/128‑1 (MQ=255)
                                                                                           gTATAAATAAATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAatat                                 <  1:5222648/128‑1 (MQ=255)
                                                                                             ataaataaATGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTa                               >  1:4579149/1‑128 (MQ=255)
                                                                                             ataaataaATGTTGAAAATTTAGTTTGCATACTAACAGTAAAATAAGAAATAATAAACTCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTa                               <  2:2361191/128‑1 (MQ=255)
                                                                                              taaataaaTGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTAc                              >  2:1656243/1‑128 (MQ=255)
                                                                                                aataaatGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCTGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACtt                            >  2:5817500/1‑128 (MQ=255)
                                                                                                aataaatGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACtt                            >  2:402474/1‑128 (MQ=255)
                                                                                                   aaatGTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTaa                         >  2:4754589/1‑128 (MQ=255)
                                                                                                       gtttcaaCATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACAt                     >  1:3922569/6‑128 (MQ=255)
                                                                                                       gTTGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAACAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACAt                     <  2:3633492/128‑1 (MQ=255)
                                                                                                        ttGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACAtt                    <  1:2579509/128‑1 (MQ=255)
                                                                                                         tGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTa                   <  2:2663512/128‑1 (MQ=255)
                                                                                                         tGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTa                   <  1:2805819/128‑1 (MQ=255)
                                                                                                         tGAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAAGATTCCTTTAAACATTa                   >  2:4444068/1‑128 (MQ=255)
                                                                                                          gAAACATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCTAAAGCAATAGCAGCTGCAGTAATAATTGCTGGATTTAAAATAGGTAATATTACTTTAAACATTAc                  <  2:2254674/128‑1 (MQ=255)
                                                                                                           aaaCATTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACt                 <  1:4185853/128‑1 (MQ=255)
                                                                                                           aaaCATTAGTGTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACt                 <  1:3791966/128‑1 (MQ=255)
                                                                                                               aTTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAACCTCATTGCAAAAGCAATAGCAGCTGAAGGAATAATTGCTGGTTTTTAAATAGGTAATATTAATTTAAACATTCCTtgat             >  2:3386446/1‑128 (MQ=255)
                                                                                                               aTTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACTtgat             >  2:3912676/1‑128 (MQ=255)
                                                                                                               aTTAGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACTtgat             >  1:4051632/1‑128 (MQ=255)
                                                                                                                  aGTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACTTgatgat          <  1:2016809/128‑1 (MQ=255)
                                                                                                                   gTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACTTGATGAtt         <  1:4675231/128‑1 (MQ=255)
                                                                                                                   gTTTGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACTTGATGAtt         <  2:5195055/128‑1 (MQ=255)
                                                                                                                     ttGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATTATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACTTGATGAtttt       >  2:5434605/1‑128 (MQ=255)
                                                                                                                      tGCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACTTGATGAttttt      <  2:5051359/128‑1 (MQ=255)
                                                                                                                       gCATACTACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTg                                                       >  1:5616365/1‑78 (MQ=255)
                                                                                                                             |                                                                                                                            
ATTAATAGCATTTCAAGTAATAATAGATAAAGCAATTACTAAAACTAAACAAGTACCAAAAACAAAAATAAATGGTCTAGTTTTTTTAGCAGTATAAATAAATGTTGAAACATTAGTTTGCATACCACCAGTAAAATAAGAAATAATAAAATCATCAAAACTCATTGCAAAAGCAATAGCAGCTGCAGTAATAATTGCTGGTTTTAAAATAGGTAATATTACTTTAAACATTACTTGATGATTTTTAGCT  >  CP016816/121105‑121354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑