Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 20,605 C→T Q64* (CAG→TAG)  JCVSYN2_00070 → tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681620,6050CT99.2% 5474.3 / NA 1850Q64* (CAG→TAG) JCVSYN2_00070tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (876/945);  minor base A (8/7);  total (896/954)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1865 total aligned reads displayed.

GCTTATTATATAACAGTGGAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAGATGGCGT  >  CP016816/20480‑20729
                                                                                                                             |                                                                                                                            
   tattctATAACAGGGGAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTgg                                                                                                                         <  1:5918304/128‑1 (MQ=255)
   taatatataACAGTGGAGCGATTACAGAATTAGGAAGCGTGGAAAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTgg                                                                                                                         <  2:1531849/125‑1 (MQ=255)
     ttatATAACAGTGGAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGa                                                                                                                       <  1:1903315/128‑1 (MQ=255)
          taACAGTGGAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATa                                                                                                                  >  1:5284815/1‑128 (MQ=255)
          taACAGTGGAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATa                                                                                                                  <  1:2194865/128‑1 (MQ=255)
            cctgTGGAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACg                                                                                                                <  1:3033473/125‑1 (MQ=255)
                tGGAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAgg                                                                                                            <  2:6443920/128‑1 (MQ=255)
                  gAGCGATTACAGAATTCGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTg                                                                                                          <  1:4483616/128‑1 (MQ=255)
                  gAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTg                                                                                                          >  2:1660042/1‑128 (MQ=255)
                   aGCTATTACAGAATTAGGAAGCGGGGACAACGGTCCAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGa                                                                                                         <  2:4442138/128‑1 (MQ=255)
                      gATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTAGAATTCAGACAGGAATATCCTCATTTTAGTGGGAAAATACGAAGGGGaccc                                                                                                      >  2:1925847/1‑125 (MQ=255)
                        ttACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGATAATACGAAGGTGAAcatc                                                                                                    >  2:756170/1‑128 (MQ=255)
                        ttACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTAAGAGAGGAATTACAATTCAGACATGAATAACCTCTATTTAGTGGGAAAATACGAAGTTGAAcatc                                                                                                    >  1:421040/1‑128 (MQ=255)
                         tACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAAcatca                                                                                                   <  2:865839/128‑1 (MQ=255)
                           cAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATa                                                                                                 <  1:4976222/128‑1 (MQ=255)
                            aGACTTCGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAg                                                                                                <  1:1521091/128‑1 (MQ=255)
                              taTTAGGACTCTTGGACAAAGGTACACCGATGACGGATAATACGTTTTTAGATCTGAAGAGAGGAATTACAATTCAGACTGGAATAACCTCTTTTTAGTGGGAAAATACGAATGTGAACATCATAGac                                                                                              <  2:6233247/127‑1 (MQ=255)
                              aaTTATGAAGCGGCGACATAGGTACAACGAGGACGGATAATTCGCTTTTAGAACGTCAGAGAGGAATTACTATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGac                                                                                              <  1:1145139/128‑1 (MQ=255)
                              aaTTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGac                                                                                              <  1:4078527/128‑1 (MQ=255)
                                ttAGGAAGAGTTGACAAATGTACAACGAGGAAGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGacac                                                                                            <  1:743911/128‑1 (MQ=255)
                                 tAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGATTTAAGAACGTCAGACAGGTAATACAATTCAGACAGGAATAACCTCTATTTAGTGTGAAAAAACGAAGCTCAACATCTTAGacact                                                                                           >  2:3435172/1‑127 (MQ=255)
                                    gAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCa                                                                                        <  1:2295477/128‑1 (MQ=255)
                                      aGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAgg                                                                                      >  2:4035130/1‑128 (MQ=255)
                                      aGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAgg                                                                                      <  2:5395265/128‑1 (MQ=255)
                                       gCGTGGACAACGGTACAACGAGGACGGATAATACGCTATTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGa                                                                                     <  1:2051800/128‑1 (MQ=255)
                                       gCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGTATAACCTCTGTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGa                                                                                     >  2:4830380/1‑128 (MQ=255)
                                       gCGTCGACAAAGGTACAACGAGGACGGATAATACGCTTTTGGAACGGCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTTGGAAAATACGAAGGTGAACATCATAGACACGCCAGGa                                                                                     <  2:3560563/128‑1 (MQ=255)
                                        cGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGTAc                                                                                    <  2:2497183/128‑1 (MQ=255)
                                        cGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGAc                                                                                    >  1:6190519/1‑128 (MQ=255)
                                        cGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGGCAGAGAGGAATTACAATTCAGACAGGAATACCCTCTGTTTAGTGGGAAAATACGCAGGTGAAGCTCATAGACCCGACCGGAc                                                                                    >  2:5120566/1‑128 (MQ=255)
                                         gTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAAcatcat                                                                                                  <  2:1814989/113‑1 (MQ=255)
                                          tGGACAACGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACat                                                                                  <  1:1358489/128‑1 (MQ=255)
                                          tGGACAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATACCCGCTTTTTAGTGGGAAAATACGAAGTTGAACATCATAGACACGCCAGGACata                                                                                 >  1:4853113/1‑128 (MQ=255)
                                           ggAGAATGGTACAACGAGGACGGATAATACGCTTTTAGAAAGTCAGAGAGTAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACata                                                                                 <  2:2439895/128‑1 (MQ=255)
                                            gACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGTGAATATACGAAGGTGATCATCATAGACACGACATGACatat                                                                                >  1:5186698/1‑128 (MQ=255)
                                            gACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACatat                                                                                >  2:5237146/1‑128 (MQ=255)
                                            gACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACatat                                                                                <  1:3981134/128‑1 (MQ=255)
                                             aCAAAGGTACAACGAGGTCGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAAATCAGACAGGAATAACCTCTTTTTAGTGGGTAAATAAGAAGGTGAACATCATAGGCCGGCCAGGAGAAATg                                                                               >  2:2611986/1‑128 (MQ=255)
                                               aaaTGTACAACGATGACGGATAATACGCTTTTAGAACGGCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGa                                                                             <  1:6007535/128‑1 (MQ=255)
                                               aaaGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACATTTCAGACAGGATTAACCTCTTTTTAGTGGGAAAATAGGAAGGTGAACATCAAAGACTCGCCAGGACATATgta                                                                             >  2:5008369/1‑126 (MQ=255)
                                                  ggTACTACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGAttt                                                                          <  2:2976623/128‑1 (MQ=255)
                                                  ggTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGGATTACAAAACAGACAGGAATAACCTCTTTTTACTGGGAAAATACGAAGGTGAACATCATAGCCGCGCCAGGACAGATGGAttt                                                                          >  1:2834338/1‑128 (MQ=255)
                                                  ggTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATATCCACGCCAGCTCATATGGAttt                                                                          >  2:5961869/1‑128 (MQ=255)
                                                    tACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCt                                                                        <  1:6295271/128‑1 (MQ=255)
                                                    tACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCt                                                                        <  1:6512750/128‑1 (MQ=255)
                                                      cAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTa                                                                      >  1:5090654/1‑128 (MQ=255)
                                                       aaCGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAg                                                                     <  1:5729574/128‑1 (MQ=255)
                                                       aaCGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAAATCAGACAGGAATAACCTCCTTTTAGAGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATAAGGATGTCTTAg                                                                     >  1:5601089/1‑128 (MQ=255)
                                                        aCGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGc                                                                    <  1:6391114/128‑1 (MQ=255)
                                                          gAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAg                                                                  >  2:129322/1‑128 (MQ=255)
                                                           aGGACGGATAATACGCTTTTAGAACGGCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGa                                                                 <  2:6081545/128‑1 (MQ=255)
                                                            ggACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGaa                                                                <  1:1678288/128‑1 (MQ=255)
                                                            ggACGGATAATACGCTGTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGaa                                                                <  1:4693999/128‑1 (MQ=255)
                                                             gACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGCCAGGATAAACCTCTTTTTAGTGGGAAAATACGAAGTTGAACATCAAAGCCACTCCACGACATATCGAATTCTAAGCCGaat                                                               >  2:4560252/1‑127 (MQ=255)
                                                              aCGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACTGGAATACCCTCTTTTTAGTGGGAAAATACGATGGTGAACAACATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGt                                                              <  2:435397/128‑1 (MQ=255)
                                                               cgtataataCGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGCCACGCCAGGACCTCGGGCGTTCTTAGCAGAAGta                                                             >  2:4223193/4‑128 (MQ=255)
                                                                ggATAATACGCTTTTAGAACGTCAGAGAGGAATTACACTTCAGACAGGAATAACCTCTTTTAAGTGGGAAAATACGAAGGTGAACCTCATAGCCCCGCCACGACATATGGATTTCTAAGCTGAAGaat                                                            >  2:390269/1‑128 (MQ=255)
                                                                 gATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGtata                                                           >  1:3154738/1‑128 (MQ=255)
                                                                    aataCGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCg                                                        >  2:3793846/1‑128 (MQ=255)
                                                                     ataCGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGGCACGCCAGGACATATGGATTTCTTAGCAGCAGTATATCGt                                                       >  1:4357386/1‑128 (MQ=255)
                                                                     ataCGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGt                                                       <  1:1818851/128‑1 (MQ=255)
                                                                     ataCGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGt                                                       >  1:2942778/1‑128 (MQ=255)
                                                                      taCGCTTTTAGAACGTCAGTGCGGAATTACTATTCAGAAAGGAATTACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATAATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGtt                                                      <  2:2757176/128‑1 (MQ=255)
                                                                      taCGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGc                                                                    >  1:3887132/1‑114 (MQ=255)
                                                                      taCGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGtt                                                      >  1:3798956/1‑128 (MQ=255)
                                                                        cGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATgg                                                                              <  2:5701435/102‑1 (MQ=255)
                                                                             ttAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATc                                               <  1:1018727/128‑1 (MQ=255)
                                                                              tAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCa                                              >  2:2867986/1‑128 (MQ=255)
                                                                               aGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAg                                             >  1:670951/1‑128 (MQ=255)
                                                                                gAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGt                                            <  1:871089/128‑1 (MQ=255)
                                                                                gAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGAAATATGGATATCTTAGCAGAAGTAGATCGGTCAGTATCAGt                                            >  1:2612542/1‑128 (MQ=255)
                                                                                gAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATAAGAAGGTGAACATCATAGACACGCCAGGACATATGCATTTCTTAGCAGAAGTATATAGTTAATTATcttt                                            >  2:548703/1‑125 (MQ=255)
                                                                                    gTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTa                                        >  1:2707570/1‑128 (MQ=255)
                                                                                     tCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAg                                       <  1:5393055/128‑1 (MQ=255)
                                                                                      cAGAGAGTAATTACAATTCAGACAGCACTAACCTATTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGa                                      <  2:2294004/128‑1 (MQ=255)
                                                                                       agagagGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGAt                                     >  2:4697707/1‑128 (MQ=255)
                                                                                         agagGAATTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTa                                                 <  1:1276769/114‑1 (MQ=255)
                                                                                               actACCATGCAGACCGCACTACCCTCTTTTTAGGGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAt                             <  1:3345285/126‑1 (MQ=255)
                                                                                               aTTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAg                                                                     <  2:4930670/88‑1 (MQ=255)
                                                                                               aTTACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGttt                                          <  2:3178202/115‑1 (MQ=255)
                                                                                               aTTACAATTCAGACAGCTTTAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAt                             <  2:3661808/128‑1 (MQ=255)
                                                                                                 tACAATTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTc                           >  2:1035113/1‑128 (MQ=255)
                                                                                                  aCAAATCAGACAGGAATAACCTCTATTAAGTGGGAAAATACGAAGGTGAACATCATAGACAAGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCt                          <  1:2492662/128‑1 (MQ=255)
                                                                                                     aTTCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTATAGATGGGGCAATTCTACt                       >  2:2192832/1‑128 (MQ=255)
                                                                                                       tCAGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGTGCAATTCTACTGa                     >  2:116886/1‑128 (MQ=255)
                                                                                                         aGACAGGAATCACCGCTTTTTCGTGGGAAAATACGAACGTGAACATCATAGCCACGCCCGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGAGGGGGCAATTCTACTGAtt                   <  1:3489958/128‑1 (MQ=255)
                                                                                                         aGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGCtt                   >  1:2384317/1‑128 (MQ=255)
                                                                                                         aGACAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGAtt                   >  2:144850/1‑128 (MQ=255)
                                                                                                           aCAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGTGCAATTCTACTGATTTc                 >  1:5480413/1‑128 (MQ=255)
                                                                                                            cAGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCt                >  2:3093131/1‑128 (MQ=255)
                                                                                                             aGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTg               <  2:328972/128‑1 (MQ=255)
                                                                                                             aGGAATAACCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTg               <  1:3596709/128‑1 (MQ=255)
                                                                                                                aaTAACTTATTTTTAGAGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCaa            <  2:2085531/128‑1 (MQ=255)
                                                                                                                   aaCCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAg         >  2:632530/1‑128 (MQ=255)
                                                                                                                    aCCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATTTCGTTCATTATCAGTTTTAGATGGGGCAATTCGACTGTTGTCttcttaata        >  1:1462700/1‑120 (MQ=255)
                                                                                                                    aCCTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAGa        <  2:5808641/128‑1 (MQ=255)
                                                                                                                     ccTCTTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCaaaa          >  2:6573192/1‑125 (MQ=255)
                                                                                                                        ctTTTTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAGATGGc    >  1:3708634/1‑128 (MQ=255)
                                                                                                                         ttttTAGTTGGAAATTAGGTAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAGATGGCg   <  1:5811054/128‑1 (MQ=255)
                                                                                                                         ttttTAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAGATGGCg   >  2:5583342/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
GCTTATTATATAACAGTGGAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAGATGGCGT  >  CP016816/20480‑20729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: