Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,791 T→A intergenic (‑100/+71) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7910TA96.6% 8503.1 / 102.1 2882intergenic (‑100/+71)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base T (45/54);  new base A (1406/1375);  total (1452/1430)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2988 total aligned reads displayed.

ATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484665‑484913
                                                                                                                              |                                                                                                                           
       aaaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaa                                                                                                                    >  1:4398638/1‑128 (MQ=255)
        aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                   <  1:500990/128‑1 (MQ=255)
           aaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTAAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCa                                                                                                                >  1:310229/1‑128 (MQ=255)
             tAGTCATAACTCATAGATTTAATAGTTTTATAGTTGACCAAATAATAAGATAGAGCATAGAAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                              <  1:1552309/128‑1 (MQ=255)
                   aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTACACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                        >  2:3188100/1‑128 (MQ=255)
                    aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAAATAAACTACACACTATTATTTAATTATATTTTTTTTTAATATAAAAACAAtataaaaac                                                                                                      >  1:4633389/1‑120 (MQ=255)
                     aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCGTAACAACTAAACAACACGCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaaacca                                                                                                      >  2:1370955/1‑124 (MQ=255)
                       tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                    <  1:4139867/128‑1 (MQ=255)
                        cATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaa                                                                                                   <  1:2925074/128‑1 (MQ=255)
                         aTAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaa                                                                                                  <  1:71666/128‑1 (MQ=255)
                              tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                             >  1:3867374/1‑128 (MQ=255)
                              tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAAATATAAAAAGAAATTAAACACACaaaaaaaa                                                                                             >  1:4248941/1‑125 (MQ=255)
                              tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGAGTATTTATTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                             >  2:2212152/1‑128 (MQ=255)
                               ttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTAAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                            <  2:1865730/128‑1 (MQ=255)
                                tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTCCACGCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACCCAAAAACTCAc                                                                                           >  2:1175560/1‑128 (MQ=255)
                                tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTAAc                                                                                           >  1:5761792/1‑128 (MQ=255)
                                     cTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                      >  1:5098930/1‑128 (MQ=255)
                                      ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                     <  1:2490422/128‑1 (MQ=255)
                                        ttATAGTTTACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACACGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAACAACACAAAAACTCACCCATTATg                                                                                   >  1:6398421/1‑128 (MQ=255)
                                        ttATAGATGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                   <  1:4548135/128‑1 (MQ=255)
                                              ttGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGt                                                                             >  2:2554130/1‑128 (MQ=255)
                                                gACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGttt                                                                           <  1:5433896/128‑1 (MQ=255)
                                                   cAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaa                                                                        >  2:4941183/1‑128 (MQ=255)
                                                    aaaataTAAGATACAGCATAGCAGAAGCTCTTAACAACTAAACAACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                       <  1:1137679/128‑1 (MQ=255)
                                                    aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                       >  1:63091/1‑128 (MQ=255)
                                                     aaaaaTAAGATAGTGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  2:505114/128‑1 (MQ=255)
                                                     aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACCCACCCATTATGGTGAGTTTaaaaa                                                                      <  1:1223716/128‑1 (MQ=255)
                                                     aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  2:160438/128‑1 (MQ=255)
                                                       aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  1:3489598/128‑1 (MQ=255)
                                                       aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  2:4073475/128‑1 (MQ=255)
                                                        aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  2:2422716/125‑1 (MQ=255)
                                                           aaGATATAGTATAGCAGAAGCTATTAACAACTAAACTACACTCGATTATTTAACTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                <  2:4202664/128‑1 (MQ=255)
                                                           aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                         <  1:5314426/119‑1 (MQ=255)
                                                           aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                               <  1:3749837/128‑1 (MQ=255)
                                                            aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                               >  1:5184314/1‑128 (MQ=255)
                                                            aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAGTATGGTGAGTTTAAAAATTATTCa                                                               >  1:6506462/1‑128 (MQ=255)
                                                             gATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaa                                                              <  1:3240476/128‑1 (MQ=255)
                                                             gATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaa                                                              <  1:3598664/128‑1 (MQ=255)
                                                              aTAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaa                                                             >  2:6360064/1‑128 (MQ=255)
                                                                agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                           >  1:1675525/1‑128 (MQ=255)
                                                                 gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          >  2:5611497/1‑128 (MQ=255)
                                                                 gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          <  1:1797356/128‑1 (MQ=255)
                                                                  agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         >  2:4564764/1‑128 (MQ=255)
                                                                   gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         <  2:3694401/128‑1 (MQ=255)
                                                                   gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                        >  2:2330465/1‑128 (MQ=255)
                                                                   gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                        >  1:1914514/1‑128 (MQ=255)
                                                                   gCATAGCAGAAGCTCTTAACAACGAAACTACACTCGATTATTAAATTATATTTTTTTTTAATATAAAAAGCAATTAACCACACAAAAACTCACCCAAAATCGTGAGTATAAAAATTATACAAAAAtta                                                        >  2:3450947/1‑127 (MQ=255)
                                                                     aTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                      >  1:2570782/1‑128 (MQ=255)
                                                                      tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  2:1478386/128‑1 (MQ=255)
                                                                        gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                   >  1:5517390/1‑128 (MQ=255)
                                                                        gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAACCCATTATCGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                   >  2:4801191/1‑128 (MQ=255)
                                                                        gCAGAAGCTATTAAAAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                   <  2:3343101/128‑1 (MQ=255)
                                                                           gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  1:818796/106‑1 (MQ=255)
                                                                           gAAGCTCATAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                <  2:4326808/128‑1 (MQ=255)
                                                                            aaGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGAATAtttt                                               >  1:2292663/1‑128 (MQ=255)
                                                                               ctctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                            <  1:278981/128‑1 (MQ=255)
                                                                                  ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTTATTATTTTAAATCa                                         >  2:888908/1‑128 (MQ=255)
                                                                                  ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATAAAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                         <  2:5416189/128‑1 (MQ=255)
                                                                                   tAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                           >  2:1996379/1‑77 (MQ=255)
                                                                                    aacaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                       <  1:5890862/128‑1 (MQ=255)
                                                                                    aacaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                       >  2:4470141/1‑128 (MQ=255)
                                                                                     acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                      <  1:23649/128‑1 (MQ=255)
                                                                                      caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTg                                     >  2:6621547/1‑128 (MQ=255)
                                                                                       aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    >  2:5303585/1‑128 (MQ=255)
                                                                                       aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    <  1:2027218/128‑1 (MQ=255)
                                                                                       aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    >  1:4002031/1‑128 (MQ=255)
                                                                                        actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   <  1:2166099/128‑1 (MQ=255)
                                                                                        actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   <  2:2910757/128‑1 (MQ=255)
                                                                                        actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   >  2:3056181/1‑128 (MQ=255)
                                                                                         ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATGGTGAGTTTAAAAAATATTCAAAAATTTGATTATTTTAAATCAATTTAAg                                  >  2:5506728/1‑128 (MQ=255)
                                                                                          taaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGc                                 >  2:6017807/1‑128 (MQ=255)
                                                                                          taaactaCACTCGATTATTAAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGc                                 <  2:1270585/128‑1 (MQ=255)
                                                                                           aaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCt                                <  1:3354211/128‑1 (MQ=255)
                                                                                            atctaCAATCGATTATTTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACTAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                               <  2:748330/126‑2 (MQ=255)
                                                                                              ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                             >  2:5916953/1‑128 (MQ=255)
                                                                                                acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                       <  1:6136869/68‑4 (MQ=255)
                                                                                                 cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                          >  1:1000667/1‑128 (MQ=255)
                                                                                                 cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                          <  2:6130623/128‑1 (MQ=255)
                                                                                                 cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                          <  2:3958948/128‑1 (MQ=255)
                                                                                                  acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                       <  2:1717297/114‑1 (MQ=255)
                                                                                                     cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                      >  1:4893480/1‑128 (MQ=255)
                                                                                                     cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                      >  2:6233581/1‑128 (MQ=255)
                                                                                                      gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                     >  2:4703214/1‑128 (MQ=255)
                                                                                                      gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                     <  1:1339278/128‑1 (MQ=255)
                                                                                                      gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTAAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                     >  2:5188529/1‑128 (MQ=255)
                                                                                                       attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                      <  2:2784934/126‑1 (MQ=255)
                                                                                                          attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                 >  1:950113/1‑128 (MQ=255)
                                                                                                          attTAATTATATTTTTATTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                 <  2:2656389/128‑1 (MQ=255)
                                                                                                            ttAATAATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt               <  2:2098496/128‑1 (MQ=255)
                                                                                                             tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa              <  1:1424936/128‑1 (MQ=255)
                                                                                                              aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg             <  1:4785370/128‑1 (MQ=255)
                                                                                                                  atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  2:5744311/1‑128 (MQ=255)
                                                                                                                  atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  1:66140/1‑128 (MQ=255)
                                                                                                                  atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  1:60034/1‑128 (MQ=255)
                                                                                                                   tataTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  2:1041840/1‑128 (MQ=255)
                                                                                                                    atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGATTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCa       >  1:5654076/1‑128 (MQ=255)
                                                                                                                    atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt               >  2:6478421/1‑120 (MQ=255)
                                                                                                                     tttttttttaATATAAAAAGCAATTAAAGACACTAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTcgt      <  2:3818431/128‑3 (MQ=255)
                                                                                                                      ttttttttaATATAAAAAGTAATTAAACACACAAAAAATAAACCATTATTGTGAGTTTAAAAATTATTAAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt     <  1:268026/128‑1 (MQ=255)
                                                                                                                      ttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt     <  2:3579550/128‑1 (MQ=255)
                                                                                                                              |                                                                                                                           
ATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484665‑484913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: