Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4870T.41.0% 585.0 / inf 641intergenic (+265/‑410)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (173/168);  new base . (58/179);  total (263/378)
Fisher's exact test for biased strand distribution p-value = 2.16e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-10

Only 100 of 682 total aligned reads displayed.

TAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACT  >  CP016816/202367‑202613
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    taataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAAAAAAAAAAAACAATACC‑‑ATtaaaaattaaaaaa                                                                                                                   >  2:6078116/1‑128 (MQ=255)
       taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCAACTT‑‑‑AAAAATaaaaaaaaaa                                                                                                               >  1:3258140/1‑128 (MQ=255)
       taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaa                                                                                                                >  2:5416959/1‑128 (MQ=255)
       taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑‑TAAAAAATtaaaaaaaaa                                                                                                               >  2:1996230/1‑128 (MQ=255)
       taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATACCC‑‑TTAAAAATTaaaaaaaaa                                                                                                                >  2:5021172/1‑128 (MQ=255)
        ttatttAACGTTAATTAAATATCACTAAACTTAATAAATTTTATAATGTTGATCCTCGGTTTCTATTTTTTTTTTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaa                                                                                                               <  2:3196434/124‑1 (MQ=255)
          agTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTTATCTATTTTTATATTTTTTTATCTTCACAAATAAAAAAAAACAATCAAC‑‑TTAAAAATTaaaaaaaaaaaa                                                                                                             >  1:2038790/1‑128 (MQ=255)
           gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAATaaaaaaaaaaaaaa                                                                                                           >  1:6012243/1‑128 (MQ=255)
           gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                            >  2:4954533/1‑128 (MQ=255)
           gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                            >  2:6348724/1‑128 (MQ=255)
            ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTAAAAAATTAAATCATCGTGATCTATTTTTAAATATTATTATCTTCAAAAATAAAAAAAAAAAATAAC‑‑AATAAAAAATaaaaaaaaaaaaaa                                                                                                           >  2:1198286/1‑128 (MQ=255)
             tAATGTTAATTAAAAAAACATAAAATTATAAATTTTTATAATTTTTATTCTCGTGTTATATTTTTTTATTTGTTTATATTCAAAAATAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                          <  2:6255277/128‑1 (MQ=255)
             tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑‑ATAAAAAATtaaaaaaaaaaaaaa                                                                                                          >  2:5097070/1‑128 (MQ=255)
             tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                          >  1:1599810/1‑128 (MQ=255)
              aattttaattaaAATAACAAAAACATAATAATTTTTATAATTTATTATCTCGTGATTTTTTTTTTTTTTTTTTTATTTTAACAAATAAAAAAAAAAAATACCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                         <  2:1606541/124‑1 (MQ=255)
              aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATACCC‑‑TTAAAAAATaaaaaaaaaaaaaaaa                                                                                                         >  2:1325188/1‑128 (MQ=255)
              aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAAT‑‑CCAATTAAAAATTaaaaaaaaaaaaaaaa                                                                                                         >  1:761184/1‑128 (MQ=255)
              aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAAAAAAAAAAAACAATCCCCTTAaaaaaaaaaaaaaaaaaaaaaa                                                                                                           >  2:1683567/1‑128 (MQ=255)
              aaGGTTAATTAAAATAACATAAACTTAATAATTATTATAATGTAGATAATATTGATCTATTTTTTTATTTGTTTATATTAACAAATAAAAAAAAAAAAT‑‑AAAATTAAAAATTaaaaaaaaaaaaaaaa                                                                                                         >  2:2455295/1‑128 (MQ=255)
               agattaattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCAAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                        >  2:3654928/4‑128 (MQ=255)
               aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                        >  2:6598191/1‑128 (MQ=255)
               aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                        >  2:834459/1‑128 (MQ=255)
                ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTATTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                       >  1:45737/1‑128 (MQ=255)
                ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                       >  2:758814/1‑128 (MQ=255)
                  ttaattaaAATACCATAAACTTAATAATTCTTATAATTTAGATCAACTTTATATAATTATATATAAAAAAATATTAAAAAATAAAAAAAAAAAAT‑‑‑CAAAAAAAAAATaaaaaaaaaaaaaaaaaaaaa                                                                                                    >  2:26926/1‑128 (MQ=255)
                   taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATACCC‑‑ATAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                                    >  2:5271334/1‑128 (MQ=255)
                   taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCATCGTGATCTAATTTTTTATTTATATATATTCACAAATAAAAAAAAACAAAAAACTTAAAAATaaaaaaaaaaaaaaaaaaaaaa                                                                                                      >  2:4580865/1‑128 (MQ=255)
                    aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATACTATTTATCTATTTTTTTAATTTTTTATCTTCACAAATAAAAAAAAACAATACACTTAAAAATAaaaaaaaaaaaaaaaaaaaaaa                                                                                                     >  1:2490181/1‑128 (MQ=255)
                     attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAAACCAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  2:2196970/1‑126 (MQ=255)
                      ttaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                 >  2:5882116/1‑125 (MQ=255)
                       taaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  2:5466765/1‑124 (MQ=255)
                       taaAATACCATAAACTTAAAAAATATTAAAATTTAGAACCTCGTGATCTTTTTTTTTATTTGTTTATATTAACAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTaaa                                                                                              <  1:6316605/128‑1 (MQ=255)
                       taaAAAACAATAAATTAAAAATTTTTATAATTTATATAATTTTTATATATTATTTTTTTTTTTTAAATTAAAAAAAAAAAAAAAAAAAACCCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTaaaa                                                                                             <  1:670057/128‑1 (MQ=255)
                        aaaataaaataaaatTAAATATTATTATAATTTATAACCTTGTTATCTATTTTTTTATTTTTTTATCTTAACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaactaaaaac                                                                                               <  1:1883182/128‑9 (MQ=255)
                        aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTAATTTATCTTCACATATAAAAAAAAACAAAACCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                               >  2:2946053/1‑123 (MQ=255)
                        aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTATTTATCTATTTTTTTATTTTTTTATATTCACAAATAAAAAAAAAAAATAACC‑‑TTAAAAAATaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                               >  1:1003267/1‑123 (MQ=255)
                         aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAACAAAAACC‑‑TTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                             >  2:3845231/1‑128 (MQ=255)
                         aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTACACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                             >  1:4957368/1‑128 (MQ=255)
                           attacATAAAATTAAAATATCTTATAATGTATATACTCTTTATCTATTATTTTATTTTTTTATCTTAAAAAATAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                          <  1:3025391/124‑1 (MQ=255)
                            tataaTAAAATTAATAATTATTATAATTTATATCCTTGTTTTTTATTTTTTTTTTTTTTTATATTAAAAAATAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                         <  2:1819405/124‑1 (MQ=255)
                             taaataaaaaTTAATTATTATTATAATTTATATAATTGTTATAATTTTTTTTTTTTTTTTATATTAAAAAAAAAAAAAAAAAAAAACCC‑‑TTTAAAATTAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                          <  1:6436430/122‑1 (MQ=255)
                               cATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACaaaaaaaaaaaa                                                                                      >  2:5724598/1‑126 (MQ=255)
                                aTAAACTTAAAAATTCTTAAAATTCTCATCCCCGTTAACTATTTTTTTATTTGTTTATCTACCCAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                   <  1:3680098/128‑1 (MQ=255)
                                   aaaaTTTATAATTATTTTAATTTATTTCATCTTTTTTTATTTTTTTTTTTGTTTTTTTTAAAAAATAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                   <  2:5346603/128‑1 (MQ=255)
                                   aaaaTTAATAATTATTATAATTTAGTACATCGTTATCTATTTTTTTATTTGTTTATATTAAAAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAt                                                                                  <  2:5325973/128‑1 (MQ=255)
                                    aaCTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAACaaacaaaatat                                                                                    >  1:5762411/1‑124 (MQ=255)
                                      tttAAAAATTTTTATAATTTATATCCTAGGTATAATTTTTTTTATTTTTTTATATTCACAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTc                                                                               <  2:4302651/127‑1 (MQ=255)
                                       taaatattTTTTAAAATTTATTTCATCTTTATATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAttt                                                                                <  1:61431/126‑1 (MQ=255)
                                          aaaatTCTAAAAATTTATATCATCTTGATATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTa                                                                            <  1:527213/126‑1 (MQ=255)
                                             attattATAATTTATATAATCTTTATATATTTTTTTATTTGTTTTTCTTAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAt                                                                        <  1:4485262/128‑1 (MQ=255)
                                             atTCTTATAAATTATATCATCTTTATCTATTTTTTTAATTTTTTATATTCAAAAATAAAAAAAAAAAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaaa                                                                          <  1:5429420/128‑1 (MQ=255)
                                               tcatataATTTAGATCCTCTGTATATATTTTTTTATTTTTTTAAATTAAAAAAAAAAAAAAAACAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAtt                                                                       <  1:5827232/125‑1 (MQ=255)
                                                 ttttaATGTACATCCTCGTGATATTTTTTTTTAATTGTTTATATTCACAAAAAAAAAAAAACAATCCCC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTccc                                                                    <  1:3355733/125‑1 (MQ=255)
                                                  tataATGTATATACTCGTTATATATTTTTTTATTTGTTTATATTAACAAAAAAAAAAAAAAAAACCCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTccc                                                                    <  1:6245234/128‑1 (MQ=255)
                                                         tacaaactattGATATATTTTTTTATTTTTTTATCTTAACAAATAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATtata                                                             <  1:1765303/118‑1 (MQ=255)
                                                           tatacTCGTTATTTATTTTTTTTATTTTTAATCTTAAAAAATAAAAAAAAAAAAACCCCC‑‑‑TAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                           <  2:4092398/124‑1 (MQ=255)
                                                           gATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAATATATTTATTAAAATTcaaaattaaaaa                                                           >  1:5589538/1‑117 (MQ=255)
                                                            attctctttatttATTTTTTTTTTTTTTTATATTAAAAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                          <  1:2413787/116‑1 (MQ=255)
                                                             tcctatttatttATTTTTTTTTTTTTTTATATTTAAAAAAAAAAAAAAAAAAAACCC‑‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                         <  2:6221679/117‑1 (MQ=255)
                                                             accTAGTTATATATTTTTTTTATTTTTATAATTAAAAAAAAAAAAAAAAAAAACCC‑‑ATTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                          <  2:305987/127‑1 (MQ=255)
                                                               atatttatatatttttttattttttTATATTAAAAAATAAAAAAAAAAAATCCCC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                      <  1:3124613/119‑1 (MQ=255)
                                                                tttttttttatttttttattttttTATATTAACAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                      <  1:5508246/120‑1 (MQ=255)
                                                                ttgttatttatttttttattttttTATCTTCACAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                     <  2:5492680/120‑1 (MQ=255)
                                                                tCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACAAAAAACAACAAATATTTATATAAATTCCAAATTAAAAcacagt                                                      >  2:6139150/1‑126 (MQ=255)
                                                                  gttatatATTTTTTTAATTTTTTAAATTAAAAAATAAAAAAAAAAAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                      <  1:1331907/122‑1 (MQ=255)
                                                                  ggtatatatttttttattttttTATATTTAAAAAAAAAAAAAAAAAATCCCATT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                                    <  2:5236276/122‑1 (MQ=255)
                                                                  ctGATCTATTTTTTTATTTTTTTTTATTAAAAAATAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                                    <  1:378186/127‑1 (MQ=255)
                                                                     atatttttttttATTTGTTTAATTAAAAAAAAAAAAAAAAAAAAAACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatt                                                 <  2:5662517/123‑1 (MQ=255)
                                                                       taaTTTTTTTATTTGTTTAAATTAAAAAAAAAAAAAAAACAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                              <  1:4422611/126‑1 (MQ=255)
                                                                       attttttttttttttttTATTTTTAAAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                               <  1:6078116/125‑1 (MQ=255)
                                                                        tATTTTTTTTTTTTTTTTTATTAAAAAAAAAAAAAAAAAAATACC‑‑ATTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAATCCCTAATATACCACattttatttt                                               <  2:3563099/128‑1 (MQ=255)
                                                                        aaTTTTTTTAATTTTTTAAAATAAAAAAAAAAAAAAAAAAAAACCC‑‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                               <  1:6533974/127‑1 (MQ=255)
                                                                         ttttttttATTTTTTTTTAATAAAAAAAAAAAAAAAAAAAAACCC‑‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                             <  1:5017227/127‑1 (MQ=255)
                                                                         atttttttatttttttATATTAAAAAATAAAAAAAAACAATCACCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                            <  1:4713289/128‑1 (MQ=255)
                                                                          tttttttatttttttaTATTAACAAATAAAAAAAAAAAATACCCTT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                            <  1:427011/128‑1 (MQ=255)
                                                                           ttttttttttttttATTTTAAAAAATAAAAAAAAAAAAAACCC‑‑‑TTCAACATTAATAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTCTTATACCACATTTTATTTTTaa                                            <  1:3904834/128‑1 (MQ=255)
                                                                           ttttttATTTTTTTTTCTTCCCAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                           <  2:3433574/128‑1 (MQ=255)
                                                                           ttttttATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTATAAATTAAAAAAAAAATAAAATAAACATAAATACAAAACTGATTTGTTAAATATACGAATTATACCAAaatttaatttataa                                           >  1:5661545/1‑124 (MQ=255)
                                                                              tttATTTTTTTTTATTAAAAAAAAAAAAAAAAAAATAAAC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                                        <  1:3542851/128‑1 (MQ=255)
                                                                               ttATTTTTTTATATTAAAAAATAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                       <  1:1463743/128‑1 (MQ=255)
                                                                                   ttttttATATTAAAAAATAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATATCACATTTTATTTTTAAAATAAGata                                   <  1:4113760/125‑1 (MQ=255)
                                                                                    tGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACTATATTTATAAAAAATACATATTAAACCAATTTTTTTTTTTAAAATAatatat                                  >  2:5798120/1‑128 (MQ=255)
                                                                                     tttttaattaacaAATAAAAAAAAACAATTCCCATT‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                                 <  1:5355512/118‑1 (MQ=255)
                                                                                      ttttatattaaaaaaaaaaaaaaaaaaATACCC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                                 <  1:521521/117‑1 (MQ=255)
                                                                                        ttaataaaaaaaaaaaaaaaaaaaaATTCCC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                               <  1:2322229/119‑1 (MQ=255)
                                                                                        tatatttaaaaaaaaaaaaaaaaaaATCCCATT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGAc                              <  1:5085059/119‑1 (MQ=255)
                                                                                         tatatttaaaaaaaaaaaaaaaaaATTCCCAT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                             <  1:1667056/120‑1 (MQ=255)
                                                                                            ataaaaaaTAAAAAAAAAAAATACAC‑‑TTATAAATTATAAAAAAAAAAAAAAAAAAACTAATTACAAAATATATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                           <  1:2627758/123‑1 (MQ=255)
                                                                                               acaAATAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatata                      <  1:3462560/128‑1 (MQ=255)
                                                                                                caAATAAAAAAAAAAAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataata                        <  1:2164567/128‑1 (MQ=255)
                                                                                                aaaaaaaaaaaaaaaaaATACCC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatat                       <  2:2317504/127‑1 (MQ=255)
                                                                                                   aataaaaaaaaaCAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACAATATTTATTAAAATTCCCTATTATACCACATTTTATTTTTAAAAAAATATATTGAAATAatatata                    >  1:6391796/4‑128 (MQ=255)
                                                                                                     taaaaaaaaaaaaTACAC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATc                  <  1:825953/127‑1 (MQ=255)
                                                                                                     taaaaaaaaaCAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACAAAAAAAAAACACAATTTCTTAAAAATACCAATTAAACCACAATTTATTTTAAAAATAAGATATTAAAATAatatatata                  >  1:5932381/2‑127 (MQ=255)
                                                                                                     aaaaaaaaaaaaATACC‑‑ATTAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatatat                   <  1:2824503/128‑1 (MQ=255)
                                                                                                          aaaaaCAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACAATATTTATTAAAATTCAAATTTATAACAAATTTTATTTTTAAAAAAATAAATTTAAaaaataattataaatta             >  2:6480724/1‑117 (MQ=255)
                                                                                                            aaaCAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAAAAATATTTCTAAAAATTCACTAAAAAACCAAATTTTAATTTTAAAATAAAATATTTACAAAaaatatataaaaaaaa           >  1:396758/1‑120 (MQ=255)
                                                                                                                aaaatcacattAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatatat          <  1:5398661/119‑1 (MQ=255)
                                                                                                                   caccccttATAAATTAAAAAATAAAAAAAAAAAAAACTAAACACAAACTCTATTTCTTATAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAAtatatata       <  2:6022799/122‑1 (MQ=255)
                                                                                                                       ccttAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATAc   <  2:5553998/126‑1 (MQ=255)
                                                                                                                         |                                                                                                                               
TAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACT  >  CP016816/202367‑202613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: