Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4850T.61.7% 208.5 / inf 640intergenic (+263/‑412)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (51/174);  new base . (187/175);  total (263/377)
Fisher's exact test for biased strand distribution p-value = 1.73e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-04

Only 100 of 693 total aligned reads displayed.

GAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATAC  >  CP016816/202357‑202612
                                                                                                                                |                                                                                                                                 
gAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCt                                                                                                                                   <  1:6457541/128‑1 (MQ=255)
  aGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCAtta                                                                                                                                 >  2:5554087/1‑127 (MQ=255)
  aGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCACCtta                                                                                                                                 >  1:5392538/1‑127 (MQ=255)
  aGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACAATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCACCtta                                                                                                                                 >  1:4410021/1‑127 (MQ=255)
   gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAACAATCCACtaaa                                                                                                                                >  2:3937970/1‑125 (MQ=255)
   gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCtaaa                                                                                                                                >  2:6648258/1‑125 (MQ=255)
   gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAAAAATAAAAAAAAACAATCCACtaaa                                                                                                                                >  2:6353162/1‑125 (MQ=255)
      aGTGTAAATAATAGAGTTAAGGTTAATAAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAACAATCACCtaaaaaa                                                                                                                             >  2:2186575/1‑122 (MQ=255)
        tgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGATTATCTTCACAAATAAAAAAAAACAATCCCAttaaaaatt                                                                                                                           >  2:5787597/1‑121 (MQ=255)
            aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑Ttaaaaattaaaa                                                                                                                   >  1:5767706/1‑128 (MQ=255)
             ataataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCtt‑‑‑aaaaataaaaaa                                                                                                                 >  2:5085059/1‑128 (MQ=255)
               aataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGAGATCTATTTATTTATATTTTTATCTTAACAAATAAAAAAAAACAATCCACTT‑‑‑AAAAATaaaaaaaa                                                                                                               >  2:4622700/1‑128 (MQ=255)
                 taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTTTTTATCTACACAAATAAAAAAAAACAATAAC‑‑ATTAAAAATTaaaaaaaaa                                                                                                              >  2:2304951/1‑128 (MQ=255)
                 taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATACCC‑‑TTAAAAATTaaaaaaaaa                                                                                                              >  2:5021172/1‑128 (MQ=255)
                    attttatgTTAAATAAAATAACATAAACTTAAAAATTATTAAAAATTATTAACACGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAACAAAACCC‑‑TTAAAAAATaaaaaaaaaaaa                                                                                                           <  1:3450324/121‑1 (MQ=255)
                     gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAATaaaaaaaaaaaaaa                                                                                                         >  1:6012243/1‑128 (MQ=255)
                     gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCACC‑‑‑TAAAAAATtaaaaaaaaaaaaa                                                                                                         >  2:1438511/1‑128 (MQ=255)
                       tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                        >  1:1599810/1‑128 (MQ=255)
                       tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTATAATTAGTATATCTTCACAAATAAAAAAAAACAATAACCATaaaaaaaaaaaaaaaaaaaaaa                                                                                                          >  2:1617541/1‑128 (MQ=255)
                       tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCATGATCTATATTTTTATTTAATTAAATACACAAATAAAAAAAAAAAAAAAC‑‑‑‑ATAAAAAATtaaaaaaaaaaaaaaa                                                                                                       >  2:3144660/1‑128 (MQ=255)
                       tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACCC‑‑TTAAAAATTaaaaaaaaaaaaaa                                                                                                         >  1:5424293/1‑128 (MQ=255)
                        aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCAATT‑‑‑AAAAATaaaaaaaaaaaaaaaaa                                                                                                      >  2:6506798/1‑128 (MQ=255)
                        aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAAACACCAT‑‑‑AAAAATaaaaaaaaaaaaaaaaa                                                                                                      >  2:6254655/1‑128 (MQ=255)
                         aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                      >  2:834459/1‑128 (MQ=255)
                         aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTATTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                      >  1:5586519/1‑128 (MQ=255)
                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                     >  2:5870503/1‑128 (MQ=255)
                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                     >  2:758814/1‑128 (MQ=255)
                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                     >  2:5263761/1‑128 (MQ=255)
                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                     >  2:5194456/1‑128 (MQ=255)
                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCAC‑‑‑ATAAAAAATtaaaaaaaaaaaaaaaaaa                                                                                                    >  2:3766958/1‑128 (MQ=255)
                            ttaattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCAC‑‑‑ATAAAAAATtaaaaaaaaaaaaaaaaaaaa                                                                                                  >  2:3030811/1‑128 (MQ=255)
                            ttaattaaAAAACCAAAAAATTAATAATTATTTTAAATTAGATACCCGTTTTATATTTTTTTTTTTTTTTTTTATAACAAATAAAAAAAAAAAATCCCC‑‑ATAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                                   <  2:4956182/128‑1 (MQ=255)
                             taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAACAATCAAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  2:6074647/1‑128 (MQ=255)
                             taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  1:5340977/1‑128 (MQ=255)
                              aattaaAATACCATAAACTTAATAATTCTTATAATGTATATACTCTTGATCTATTTTTTTATTATTTTATCTTCAAAAATAAAAAAAAAAAATAAAC‑‑ATAAAAAATaaaaaaaaaaaaaaaaaaaaaa                                                                                                 >  2:4113760/1‑127 (MQ=255)
                              aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATATTCACAAATAAAAAAAAAAAAAACCC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  2:1957089/1‑128 (MQ=255)
                              aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGATTATCTTCACAAATAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaa                                                                                                 >  1:3539803/1‑127 (MQ=255)
                              aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATACTATTTATCTATTTTTTTAATTTTTTATCTTCACAAATAAAAAAAAACAATACACTTAAAAATAaaaaaaaaaaaaaaaaaaaaaa                                                                                                   >  1:2490181/1‑128 (MQ=255)
                               attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaataaaaa                                                                                                >  2:1738058/1‑126 (MQ=255)
                               attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  1:6554536/1‑126 (MQ=255)
                                 taaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑‑TAAAAAATaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                             >  2:6216569/1‑123 (MQ=255)
                                 taaAATACCATAAACTTAATAATTATTATAATGTAGATCATCTTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                              >  1:3759719/1‑124 (MQ=255)
                                  aaaataaaatAAACATAATAATTATTATAATTTATATTATAGTGTTTTATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTaaaa                                                                                            <  2:2396420/128‑1 (MQ=255)
                                  aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTATTTATCTATTTTTTTATTTTTTTATATTCACAAATAAAAAAAAAAAATAACC‑‑TTAAAAAATaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                             >  1:1003267/1‑123 (MQ=255)
                                     aaaacaaaaaaTTAATAATTATTAAAAAGTATAAACTCGTTATCTATTTTTTTATTTGTTTAAATTAACAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                        <  1:3026309/121‑1 (MQ=255)
                                      tataaTAAAATTAATAATTATTATAATTTATATCCTTGTTTTTTATTTTTTTTTTTTTTTATATTAAAAAATAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                        <  2:1819405/124‑1 (MQ=255)
                                      taacaaaaaaataataaTTCTTATAAATTATTAACTCGTTTTTTATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                        <  1:3903601/117‑1 (MQ=255)
                                      tACAATAAAATTAATAATTATTATAATTTATAAAATATTTTTATATTTTTTTATTTTTTTATATTAAAAAATAAAAAAAAAAAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                         <  1:2250896/128‑1 (MQ=255)
                                         caaaaaCTTAATAATTAATATAAATTAGATCCTAGTGAACTATTTTTTTATTTGTTTATCTTAACAAAAAAAAAAAAACAAACCCC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                                   <  1:4454431/125‑1 (MQ=255)
                                           ataaattAATAATTATTATAATGTTTAATCTTGTTATATATTTTTTTATTTTTTAATATTAAAAAATAAAAAAAAAAAAAACCC‑‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                  <  2:5451092/123‑1 (MQ=255)
                                           aaaaCTTATAAATTTATATAATTTATAACCTAGTGATATATTTTTTTATTTTTTTATCTTAAAAAATAAAAAAAAAAAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAAct                                                                                     <  1:5933305/127‑1 (MQ=255)
                                            aaaaTTCATAATTCTTAAAAATTAGACACTATTGATATATTTTTTTATTTTGTTATATACACAAAAAAAAAAAAAAAAACACCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                  <  1:5270673/128‑1 (MQ=255)
                                             aaCTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTTTCTTCACAAATAAAAAAAAACAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAACCAAacacaattt                                                                                     >  2:2919190/1‑125 (MQ=255)
                                              aattAAAAATTAATATAATTTATATAATCTTGATATTTTTTTTTATTTTTTTATCTTAACAAAAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAt                                                                                 <  1:706340/126‑1 (MQ=255)
                                              aCTTAATAATTATAATAATTTAATACCTCGTTAAATATTTTTTTTTTTGTTTATATTAACAAATAAAAAAAAAAAATACCCTT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTc                                                                              <  1:3324516/128‑1 (MQ=255)
                                                 taataaTTATTAAAATTAAGATCCTCTTTTTAAATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAACAACTAAAAACAAACTCTATTTCtt                                                                            <  2:125896/128‑1 (MQ=255)
                                                       ttCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAATAAAAACAACATAATTTTCTTaaatt                                                                       >  2:5999979/1‑126 (MQ=255)
                                                       ttCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAATCTAttaaataaaaa                                                                       >  1:4985859/1‑119 (MQ=255)
                                                             taATTTATATCCTATTGTTCTATTTTTTTATTTGTTTATATTAAAAAATAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtat                                                                <  1:2383299/128‑1 (MQ=255)
                                                              aaTGTAGATCCTCCTTATCTATTTTTTTTTTTTTTTATCTTAAAAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt                                                               <  2:3626619/128‑1 (MQ=255)
                                                                agtattatcaattGATCTATTTTTTTTTTTTTTTATATTAAAAAAAAAAAAAAAAAAATCAAC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                             <  1:1767023/116‑1 (MQ=255)
                                                                  tataaccTCGTGATATATTTTTTTATTTGTTAATATTAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATtata                                                            <  2:5310172/123‑1 (MQ=255)
                                                                  tataTCCTCTTGTTCTTTTTTTTTAATTTTTTATATTAAAAAATAAAAAAAAAAAATTCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtat                                                                <  1:6086729/125‑1 (MQ=255)
                                                                   attacatagttaTCTATTTTTTTATTTTTTTATATTAAAAAATAAAAAAAAACAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                             <  1:6405270/117‑1 (MQ=255)
                                                                   agtaccTCGTTATATATTTTTTTATTTGTTTATCTTAAAAAATAAAAAAAAACAATCCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                          <  1:2134180/124‑1 (MQ=255)
                                                                       ccTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAACAA‑‑AAAAACAACAAATAATTCTTAAAATAACCATTATAcccccaa                                                     >  2:3528401/1‑123 (MQ=255)
                                                                        ctagttatatataTTTTTATTTTTTTATCTTCACAAAAAAAAAAAAACAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACat                                                      <  1:5848661/119‑1 (MQ=255)
                                                                         ttgttatttatttttttattttttTATCTTCACAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                    <  2:5492680/120‑1 (MQ=255)
                                                                         tcttGATCTATTTTTTTTTTTTTTTATATTAAAAAAAAAAAAAAAAAAAA‑‑CCCATTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                     <  1:5068706/125‑1 (MQ=255)
                                                                         tatttttattttttttttttttttTATATTTAAAAATAAAAAAAAAAAATTCACTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                     <  1:4682058/118‑1 (MQ=255)
                                                                         tagtgttatatttttttattttttTATCTTAACAAATAAAAAAAAAAAATCACATT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                    <  1:3139948/120‑1 (MQ=255)
                                                                         tCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACAAAAAACAACAAATATTTATATAAATTCCAAATTAAAAcacagt                                                     >  2:6139150/1‑126 (MQ=255)
                                                                          tttGATATATTTTTTTATTTTTTTATATAAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                    <  2:5409584/126‑1 (MQ=255)
                                                                          ctgtttatttttttttATTTTTTTTAATTAAAAAATAAAAAAAAAATATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                    <  2:571503/119‑1 (MQ=255)
                                                                           gttatatATTTTTTTAATTTTTTAAATTAAAAAATAAAAAAAAAAAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                     <  1:1331907/122‑1 (MQ=255)
                                                                               tatatttttttattttttTAAATACAAAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                            <  1:2005743/126‑1 (MQ=255)
                                                                                ttatttttttattttttTATATTAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                              <  2:2536845/127‑1 (MQ=255)
                                                                                tatattttttttttttttttATTCAAAAATAAAAAAAAAAAATCCCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                              <  2:431668/124‑1 (MQ=255)
                                                                                cTATTTTTTTATTTTTTTAACTTAACAAATAATAAAAAACAAACCCCTT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                              <  1:4946361/128‑1 (MQ=255)
                                                                                 tatttttttattttttTATAATAAAAAATAAAAAAAAAAAATCCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                             <  1:3972754/128‑1 (MQ=255)
                                                                                  tttttttttttttttTATATTAAAAAATAAAAAAAAACAATACCC‑‑‑TAAAAAATATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                             <  1:550089/127‑1 (MQ=255)
                                                                                   tttttttATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACTTTTTATAAAAATAACAAATTAAACCAAAATTTATTTTTaa                                           >  2:5646504/1‑128 (MQ=255)
                                                                                     ttttttttttttTATATTAAAAAAAAAAAAAAAACAAAACC‑‑ATTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaaa                                         <  1:4261651/128‑1 (MQ=255)
                                                                                        ttATTTTTTTATATTAAAAAATAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                      <  1:1463743/128‑1 (MQ=255)
                                                                                        ttATTTTTTTATTTTAAAAAAAAAAAAAAAAAAATAACATT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                      <  1:834459/128‑1 (MQ=255)
                                                                                         tATTTTTTTATCATAACAAAAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                      <  1:6252229/128‑1 (MQ=255)
                                                                                            ttttttATATTAAAAAATAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATATCACATTTTATTTTTAAAATAAGata                                  <  1:4113760/125‑1 (MQ=255)
                                                                                                  tatatttaaaaaaaaaaaaaaaaaATTCCCAT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                            <  1:1667056/120‑1 (MQ=255)
                                                                                                  tatataaaaaaaaaaaaaaaaaaaaaaaCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                                <  2:5338097/120‑1 (MQ=255)
                                                                                                  ataataacaAATAAAAAAAAAAAATACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                            <  1:6348724/122‑1 (MQ=255)
                                                                                                   tCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAATAAAAACAAAAAATATTTAATAAAATTACAAATTAAAAAACAATTTATTTATAAAATAATATAtttaaaa                           >  1:6178682/1‑123 (MQ=255)
                                                                                                   atcttaaaaaaaaaaaaaaaaaaATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                            <  1:3619889/121‑1 (MQ=255)
                                                                                                    cTTCACAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACAAAAAAAAACAAGAATTTTATAAAATACCCAAATAAACCACATGTTATTTTTAAAAAAAAAATTTGACaaaa                         >  2:3403449/1‑128 (MQ=255)
                                                                                                     ataacaAATAAAAAAAAAACAAACACCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataa                         <  1:456897/125‑1 (MQ=255)
                                                                                                     ataaaaaaTAAAAAAAAAAAATACAC‑‑TTATAAATTATAAAAAAAAAAAAAAAAAAACTAATTACAAAATATATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                          <  1:2627758/123‑1 (MQ=255)
                                                                                                      taaaaaaaaaaaaaaaaaaaaaCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataa                         <  1:3269969/124‑1 (MQ=255)
                                                                                                         aaaaaaaaaaaaaaaCAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatata                     <  1:5655583/127‑1 (MQ=255)
                                                                                                          aaaataaaaaaaaaCAATACACTTAATACTTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataat                        <  1:782996/123‑1 (MQ=255)
                                                                                                              aaaaaaaaaaaaATACC‑‑ATTAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatatat                  <  1:2824503/128‑1 (MQ=255)
                                                                                                                     aaaCAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAATAAAAACAAACTAATTTTATTAAAATTACATTTTTTACCAAATTTTTTTTTAAAAAATATATATTTAAATAATATATATATtataat          >  1:5208384/1‑126 (MQ=255)
                                                                                                                                |                                                                                                                                 
GAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATAC  >  CP016816/202357‑202612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: