Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 516,362 T→C intergenic (+193/+313) JCVSYN2_02375 → / ← JCVSYN2_02380 antitermination protein NusG/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816516,3620TC98.8% 8735.0 / NA 2424intergenic (+193/+313)JCVSYN2_02375/JCVSYN2_02380antitermination protein NusG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (1103/1286);  minor base A (19/10);  total (1122/1302)
Fisher's exact test for biased strand distribution p-value = 4.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2446 total aligned reads displayed.

AAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTTAATAA  >  CP016816/516237‑516486
                                                                                                                             |                                                                                                                             
aaaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAacat                                                                                                                             <  1:1941825/128‑1 (MQ=255)
    aTAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAAAATTTTa                                                                                                                         >  2:3334709/1‑128 (MQ=255)
     tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAATAAACATTTTAg                                                                                                                        >  1:3993336/1‑128 (MQ=255)
     tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAg                                                                                                                        >  1:772273/1‑128 (MQ=255)
     tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAg                                                                                                                        <  1:175662/128‑1 (MQ=255)
     tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAg                                                                                                                        >  1:1456062/1‑128 (MQ=255)
       aaaTATATTTTTAAAAAAAATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTa                                                                                                                      <  1:425277/128‑1 (MQ=255)
        aaaatttttttAAAAAATATTTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAt                                                                                                                     <  1:3102824/122‑1 (MQ=255)
        aaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTTAAATTTTAATAAAAAAACATTTTAGTAt                                                                                                                     >  1:4488274/1‑128 (MQ=255)
         atatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAtt                                                                                                                    >  2:3162735/1‑128 (MQ=255)
          tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                                   <  2:3661735/128‑1 (MQ=255)
           atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGTGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                                  >  1:3185858/1‑128 (MQ=255)
           atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                                  <  2:1538787/128‑1 (MQ=255)
             atTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                                   <  2:4354522/125‑1 (MQ=255)
                tttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGATTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAtt                                                                                                             <  2:2912979/128‑1 (MQ=255)
                  tAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtat                                                                                                           >  1:4340495/1‑128 (MQ=255)
                   aaaaaaTATTTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtata                                                                                                         <  1:2175132/128‑1 (MQ=255)
                   aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtata                                                                                                         >  2:4503680/1‑128 (MQ=255)
                   aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTCTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtata                                                                                                         >  2:208223/1‑128 (MQ=255)
                     aaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaa                                                                                                       >  1:4108374/1‑128 (MQ=255)
                       aaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTAAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTAGCAATTATataaa                                                                                                     >  1:2613274/1‑128 (MQ=255)
                        atatGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAATGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaaaaa                                                                                                    <  2:1875549/128‑1 (MQ=255)
                         tatGTTTTTATAAGATTAGAAAAAAAGATTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAg                                                                                                   <  2:1654927/128‑1 (MQ=255)
                          atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                  <  1:2356577/128‑1 (MQ=255)
                          atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                  <  2:1778370/128‑1 (MQ=255)
                          atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                  <  1:3465633/128‑1 (MQ=255)
                          atGTTTTTAGAATATTAGAAAATAAGCTTATTTTAAGGGCTTTTTTAAATATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                  <  1:2098317/128‑1 (MQ=255)
                              ttttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTAt                                                                                              >  2:348613/1‑128 (MQ=255)
                              ttttATAAGAGAAGAAAATAAGCATATATTAAGGGCTTTTTAAAATATTATTTAAAAATTTGATTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTAt                                                                                              <  1:1370135/128‑1 (MQ=255)
                                 aatcaGATAAGAATAAAACGATATACTAAGGGCAATTTTAAAGATTATTTAAAATTTTGTTGTTATATTAAATTAAAAAGTTAATATAATATCATTTAAGGATTTGCACTTATAAAATTGTTTATGat                                                                                           <  1:2456752/124‑1 (MQ=255)
                                   taAGATTAGAAAATAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataa                                                                                         <  1:4402329/128‑1 (MQ=255)
                                   taAGATTAGAAAAACAGCTTATATTAAGGGCTTTTTTAAAGATTATTTAAAAATTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataa                                                                                         <  1:1314949/128‑1 (MQ=255)
                                   taAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataa                                                                                         <  1:957305/128‑1 (MQ=255)
                                     aGATTAGAAAAAAAGCTTATTTTAAGTGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                                       <  2:4047666/128‑1 (MQ=255)
                                     aGATTAGAAAAAAAGATTATTTTAAGGGCTTTTTTAAAGATTATTTAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATATGCAATTATAAAAAAAGTTTATGataata                                                                                       <  1:850380/128‑1 (MQ=255)
                                       aTTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAAtata                                                                                     <  2:1270983/128‑1 (MQ=255)
                                        ttAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                                    <  2:3586780/128‑1 (MQ=255)
                                            aaaataaGCTTATTTTAATTGATTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATATTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTAt                                                                                <  2:1129596/128‑1 (MQ=255)
                                             aaaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTAtt                                                                               <  1:3649873/128‑1 (MQ=255)
                                                aaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTATAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAat                                                                            <  1:1148080/128‑1 (MQ=255)
                                                aaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAat                                                                            >  1:562103/1‑128 (MQ=255)
                                                  aGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAatat                                                                          >  2:1027885/1‑128 (MQ=255)
                                                    cTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAg                                                                        <  1:1562981/128‑1 (MQ=255)
                                                     ttATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGa                                                                       >  1:3539121/1‑128 (MQ=255)
                                                      tATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAAAATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGaa                                                                      >  2:2328091/1‑128 (MQ=255)
                                                       aTTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                                     >  1:2017043/1‑128 (MQ=255)
                                                         tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                                   >  2:1965450/1‑128 (MQ=255)
                                                         tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                                   <  2:1447980/128‑1 (MQ=255)
                                                         tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACATATTAATATAGAATaa                                                                   >  1:339812/1‑128 (MQ=255)
                                                          ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATATAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                                  >  1:3917961/1‑128 (MQ=255)
                                                          ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                                  >  1:4183656/1‑128 (MQ=255)
                                                           tAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaac                                                                 >  1:1667705/1‑128 (MQ=255)
                                                              gggCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaacaac                                                              <  2:4563278/128‑1 (MQ=255)
                                                                gCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTAATAATATAGAATAAACAACTa                                                            <  2:2618216/128‑1 (MQ=255)
                                                                  ttttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGa                                                          <  2:663425/128‑1 (MQ=255)
                                                                   tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                         <  2:3853321/128‑1 (MQ=255)
                                                                   tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                         >  2:1364640/1‑128 (MQ=255)
                                                                   tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                         >  1:1221255/1‑128 (MQ=255)
                                                                    ttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaa                                                        <  2:2522098/128‑1 (MQ=255)
                                                                     tttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaaa                                                       <  2:831600/128‑1 (MQ=255)
                                                                      ttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaaa                                                       <  2:576109/127‑1 (MQ=255)
                                                                       tAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAaga                                                     <  2:2386602/128‑1 (MQ=255)
                                                                        aaaGATTATTTAAAATTTTGTTTTTATTTATAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAagag                                                    <  1:3021303/128‑1 (MQ=255)
                                                                          aGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGaa                                                  <  1:1084623/128‑1 (MQ=255)
                                                                               attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             >  2:2206525/1‑128 (MQ=255)
                                                                               attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             >  2:1208086/1‑128 (MQ=255)
                                                                               attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             >  2:2456860/1‑128 (MQ=255)
                                                                               attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             >  1:3818302/1‑128 (MQ=255)
                                                                                 ttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATAAATATATAATAAACAACTAGAAAAGAGAAAtattat                                           >  2:225988/1‑128 (MQ=255)
                                                                                  tAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                          >  2:277242/1‑128 (MQ=255)
                                                                                     aaTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGt                                       >  1:2268775/1‑128 (MQ=255)
                                                                                      aTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTa                                      >  2:3111330/1‑128 (MQ=255)
                                                                                       ttttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATTCCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTAt                                     <  1:1000225/128‑1 (MQ=255)
                                                                                       ttttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTAt                                     <  1:1850933/128‑1 (MQ=255)
                                                                                        tttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATg                                    <  1:3745106/128‑1 (MQ=255)
                                                                                         ttGATTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTTCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTGGTATGa                                   <  1:470240/128‑1 (MQ=255)
                                                                                          tGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGAt                                  >  2:926647/1‑128 (MQ=255)
                                                                                          tGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGAt                                  <  1:2867539/128‑1 (MQ=255)
                                                                                             ttttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGc                               <  2:441849/128‑1 (MQ=255)
                                                                                               ttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTTCAATTATAAAAAAGTTTATTATAATATACCTATTAATATATAATAAACAACTATAATAGAGAAATATTATTAGTATGATAGCGa                             >  2:4163094/1‑128 (MQ=255)
                                                                                               ttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGa                             >  2:3399443/1‑128 (MQ=255)
                                                                                                   ttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGATTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaatta                         <  1:695738/128‑1 (MQ=255)
                                                                                                    tttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaattaa                        <  1:2716730/128‑1 (MQ=255)
                                                                                                      taattaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaattaatt                      >  2:2026615/1‑128 (MQ=255)
                                                                                                       aattaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttt                     >  2:481740/1‑128 (MQ=255)
                                                                                                        attaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaattaattat                    <  2:2847972/128‑3 (MQ=255)
                                                                                                        attaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAtttt                    <  1:2550958/128‑1 (MQ=255)
                                                                                                        attaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAtttt                    <  1:2793526/128‑1 (MQ=255)
                                                                                                        attaaAATTTTAATAAAAAAACATTTTAGTATTTGAAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAtttt                    <  1:3334709/128‑1 (MQ=255)
                                                                                                         ttaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttttt                   <  2:270344/128‑1 (MQ=255)
                                                                                                          taaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTa                  <  1:327169/128‑1 (MQ=255)
                                                                                                            aaaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGATTAATCAACTAGAAAAGAGAAATATTATTAGTATGATAGCGATTTTATTTTTaaa                >  2:4271321/1‑128 (MQ=255)
                                                                                                            aaaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaa                <  2:3024137/128‑1 (MQ=255)
                                                                                                             aaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaaa               >  2:3953961/1‑128 (MQ=255)
                                                                                                                tttAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAattt            >  2:3248509/1‑128 (MQ=255)
                                                                                                                   aataaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAatttatt         <  2:738962/123‑1 (MQ=255)
                                                                                                                     taaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTAtttt       <  2:3735011/125‑1 (MQ=255)
                                                                                                                     taaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTAtttt       <  1:62857/125‑1 (MQ=255)
                                                                                                                     taaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTAtttt       <  1:4562337/125‑1 (MQ=255)
                                                                                                                          aaaCATTTTAGTATTTGCAAATATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaataa  <  1:2948874/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                             
AAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTTAATAA  >  CP016816/516237‑516486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: