Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,795 A→T intergenic (‑104/+67) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7950AT98.8% 6354.0 / NA 2017intergenic (‑104/+67)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (18/6);  new base T (956/1037);  total (974/1043)
Fisher's exact test for biased strand distribution p-value = 1.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2067 total aligned reads displayed.

TAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCT  >  CP016816/484671‑484919
                                                                                                                             |                                                                                                                            
  aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTaatataaaa                                                                                                                         >  1:2229139/1‑128 (MQ=255)
  aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                         >  2:1319605/1‑128 (MQ=255)
  aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACAAAAAAATAAGATAGAGCATAGCAGATGCTCTTAACAACTTAAATACACTCGATTATTTAATTATTATTTTTTTTaatataaata                                                                                                                        >  1:2619852/1‑126 (MQ=255)
     aaTAGTCAAAACTCATAGATTTAATCCTTTTATAGATGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCa                                                                                                                      <  1:3772221/128‑1 (MQ=255)
      aTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCaa                                                                                                                     >  1:176956/1‑128 (MQ=255)
       tAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                                    >  1:1413447/1‑128 (MQ=255)
         gTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTTATTAAATATATTTTTTTTTAATATAAAATGCAATTa                                                                                                                  >  1:2307790/1‑128 (MQ=255)
           cAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                                <  2:1005892/128‑1 (MQ=255)
            aaaaCTCATATATTTAATCATTTAATAGTAGACAAAAAAATAATATATAGCATAGCAGAAGCTATTAACAACTAAACTACACTCGAATATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                              <  2:3092710/128‑1 (MQ=255)
            aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATATAGCATATCAGAAGCTCTTAACAACTAAACAACACTCGATTATTTAAATATATTTATTTTTAatataaaaagaaatataac                                                                                                               >  2:2919655/1‑118 (MQ=255)
            aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                               <  2:1986256/128‑1 (MQ=255)
             aaaCTCATAGAATTAATCCTTTTATACTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                              <  1:254877/128‑1 (MQ=255)
                  cATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaa                                                                                                         <  2:2506045/128‑1 (MQ=255)
                     aGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaaa                                                                                                      <  1:1634017/128‑1 (MQ=255)
                       aTTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACt                                                                                                    <  2:21411/128‑1 (MQ=255)
                        tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAAATTTAAAAGCAATAAAACACACAAAAACGCa                                                                                                  >  1:3879084/1‑128 (MQ=255)
                         ttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                                  <  2:2753122/128‑1 (MQ=255)
                         ttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                                  >  2:1636153/1‑128 (MQ=255)
                          tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                                 >  1:412625/1‑128 (MQ=255)
                          tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                                 >  1:472488/1‑128 (MQ=255)
                           aaTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcc                                                                                                >  1:4479413/1‑128 (MQ=255)
                            cactttttATAGTTGAACAAACAATCAGATAGAGAATATCAGATGCGCTTCATAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                               <  1:1485796/124‑2 (MQ=255)
                            aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccc                                                                                               >  1:4281652/1‑128 (MQ=255)
                              ccTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                             <  1:654570/128‑4 (MQ=255)
                              ccTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                             <  2:2595164/128‑4 (MQ=255)
                               cTTATATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                            <  2:144850/128‑1 (MQ=255)
                                ttttATAGTTGACCAAAAAATAAGATAGAGCATAGGAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataat                                                                                           <  2:1259836/128‑6 (MQ=255)
                                 tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                          <  2:2859228/128‑1 (MQ=255)
                                 tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTGTTTATTATAAAAATAAATTAAACAAACAAAAACACCCCCATGAt                                                                                          >  1:2387897/1‑128 (MQ=255)
                                  ttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                         >  2:49146/1‑128 (MQ=255)
                                   tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACACATTATgg                                                                                        >  2:4620423/1‑128 (MQ=255)
                                    ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataatgggt                                                                                       <  1:1301578/128‑10 (MQ=255)
                                     taGTTGACCAAAAAATAAGCTAGATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATCTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                       <  1:2450785/128‑1 (MQ=255)
                                      agatGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACAAAACTCGATAATTTAATCATATTTTTTTTTAATATAAAAAGAAATTAAACTCACAAAAACTCCCCCAtaaaagtga                                                                                     >  1:2024291/4‑120 (MQ=255)
                                      aGTTGACCAAAAAATAAGATAGAGCATAGCAGCAGCTCTTAACAACTCAACTACACTCGATTATTTAATTAGAGTTTTTTTTATTATTAAAAGCCATTAAGCACACAAAAACTCACCCAGTATGGGGa                                                                                     >  1:3067724/1‑128 (MQ=255)
                                      aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGa                                                                                     >  2:326595/1‑128 (MQ=255)
                                          gACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGttt                                                                                 >  2:3541275/1‑128 (MQ=255)
                                              aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTAaaaa                                                                             >  1:295485/1‑128 (MQ=255)
                                              aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGAATATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                             <  2:4294161/128‑1 (MQ=255)
                                               aaaaaTAAGATATAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                            <  2:238756/128‑1 (MQ=255)
                                               aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACGCAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                            <  1:104736/128‑1 (MQ=255)
                                                aaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAat                                                                           <  2:4117698/128‑1 (MQ=255)
                                                  aaTAAGATAGAGCATATCAGAAGCTCTTAACATATAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatta                                                                         <  1:829646/128‑1 (MQ=255)
                                                   aTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAattat                                                                        >  1:1689657/1‑128 (MQ=255)
                                                           gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                                >  1:325155/1‑128 (MQ=255)
                                                           gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTAATTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                                <  2:3170582/128‑1 (MQ=255)
                                                            agCATAGCAGAAGCTCTTAACAACTAAACTTCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAAAAACAAAAACTCACACATTAAGGTGAGTATAAAAATTATTCAAAAAtt                                                               >  1:2870531/1‑128 (MQ=255)
                                                            agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                            >  1:1121988/1‑99 (MQ=255)
                                                            agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                               >  1:2796098/1‑128 (MQ=255)
                                                            agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                               >  2:4036344/1‑128 (MQ=255)
                                                            agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                               >  1:1844750/1‑128 (MQ=255)
                                                            agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                               >  2:551880/1‑128 (MQ=255)
                                                            agCATAGCAGAAGCTCTTAACAACAAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAAGGTGAGTTAAAAAATTATTCAAAAAtt                                                               >  2:3788309/1‑128 (MQ=255)
                                                                tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                           <  2:1880781/128‑1 (MQ=255)
                                                                 aGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAAACACAAAAACTCACCCAATATGGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                          >  1:3334642/1‑128 (MQ=255)
                                                                   cAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAACCACACAAAAACTCACACATTATGGTGATTTTAAAAATTATTCAAATATTTGattat                                                        >  2:2304046/1‑128 (MQ=255)
                                                                    aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaa                                                                                                        <  2:3382639/79‑1 (MQ=255)
                                                                    aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                       >  1:897764/1‑128 (MQ=255)
                                                                     gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                      >  1:4576175/1‑128 (MQ=255)
                                                                         ctctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                                  <  1:1232984/128‑1 (MQ=255)
                                                                          tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                                <  2:2658130/128‑1 (MQ=255)
                                                                          tctTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAAt                                                 <  1:435654/128‑1 (MQ=255)
                                                                           ctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                                >  1:1938608/1‑128 (MQ=255)
                                                                               acaacTAAACTTCACTCTATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                            <  2:1535365/128‑1 (MQ=255)
                                                                                 aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                          <  2:729927/128‑1 (MQ=255)
                                                                                  actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                         >  1:3170732/1‑80 (MQ=255)
                                                                                   ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                        <  2:3464343/128‑1 (MQ=255)
                                                                                    taaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGc                                       >  1:1004131/1‑128 (MQ=255)
                                                                                      aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                                     <  2:1716343/128‑2 (MQ=255)
                                                                                      aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                                     <  1:155670/128‑2 (MQ=255)
                                                                                      aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                                     <  2:1095358/128‑1 (MQ=255)
                                                                                      aactaCAATAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                                     <  1:748623/128‑1 (MQ=255)
                                                                                       actaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTcc                                    >  1:40814/1‑128 (MQ=255)
                                                                                        ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                             >  2:2079512/1‑67 (MQ=255)
                                                                                        ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                                   >  2:4474510/1‑128 (MQ=255)
                                                                                         taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                                  >  1:3695996/1‑128 (MQ=255)
                                                                                          acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAATCTCACCCATTATGGTGAGTTTAAAAATTATTCATAAATTTGATTATTTTAAATCAATTGAAGCACCatc                                 >  1:3493541/1‑126 (MQ=255)
                                                                                          acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                 >  2:3950256/1‑128 (MQ=255)
                                                                                          acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                 <  2:1191419/128‑1 (MQ=255)
                                                                                          acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                 <  2:2206623/128‑1 (MQ=255)
                                                                                           cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCATTTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAATAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                                >  1:562485/1‑128 (MQ=255)
                                                                                            acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAATCACACAAAAACTCACCCATTATGGTGAGTTTAAATATTATTCAAAAATTTGATTATTTAAATTCAATTGAAGCTCCAGCtt                               >  1:3413763/1‑128 (MQ=255)
                                                                                            acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                      >  2:4559808/1‑89 (MQ=255)
                                                                                            acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                               >  2:630480/1‑128 (MQ=255)
                                                                                            acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAGTTTAAATCAATTGAAGCTCCAGCtt                               >  2:3001596/1‑128 (MQ=255)
                                                                                              tCGATTATTTAATTATATTTATTTTAAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                             <  1:3593908/128‑1 (MQ=255)
                                                                                              tCGATTATTTAAATATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                             <  2:3622370/128‑1 (MQ=255)
                                                                                                gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCacc                           >  1:2136964/1‑126 (MQ=255)
                                                                                                      ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAATTAAttt                     <  1:4008499/128‑1 (MQ=255)
                                                                                                      ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                     <  2:3691957/128‑1 (MQ=255)
                                                                                                          ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCt                 <  2:892632/128‑1 (MQ=255)
                                                                                                           tataTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                         <  2:819968/103‑1 (MQ=255)
                                                                                                            atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt               >  2:4189311/1‑128 (MQ=255)
                                                                                                            atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt               >  2:3862921/1‑128 (MQ=255)
                                                                                                            atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt               >  1:418986/1‑128 (MQ=255)
                                                                                                              atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCa             >  2:1776699/1‑128 (MQ=255)
                                                                                                                ttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt           <  2:4370441/128‑1 (MQ=255)
                                                                                                                 tttttttaATATAATAAGAAATTAAACACACAATAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt          <  1:1187332/128‑1 (MQ=255)
                                                                                                                  ttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttc         <  1:2695440/128‑1 (MQ=255)
                                                                                                                  ttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttc         <  1:3951736/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                            
TAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCT  >  CP016816/484671‑484919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: