Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4940AT56.3% 9.1 / 310.8 382intergenic (+272/‑403)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (41/126);  new base T (85/130);  total (126/256)
Fisher's exact test for biased strand distribution p-value = 2.14e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-04

Only 100 of 447 total aligned reads displayed.

ATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTATATTGC  >  CP016816/202370‑202620
                                                                                                                                |                                                                                                                                 
aaaaaaactttATGGTTTATTAAAATCACATTAAATTATAAATTATTATAATGTAGATCCTGGTGATCTTTTTTTTTATTTGTTTATCATAAAAAATAAAAAAAAAACAAACCCC‑‑Ttaaaaattaaaa                                                                                                                             <  1:1046349/119‑1 (MQ=255)
  aataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATATTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑AAtaaaaaataaaaaa                                                                                                                           >  2:4222208/1‑128 (MQ=255)
    taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTAGTGATCTATTTTTTTATTTGTTTATATTCACAAAAAAAAAAAAAAAAATAAC‑‑‑ATAAAAAATaaaaaaaaa                                                                                                                        >  1:283693/1‑128 (MQ=255)
       agTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaa                                                                                                                      >  1:3670262/1‑128 (MQ=255)
        gTTAAGGTTAATTAAAATACCATAAACTAAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAACAATCCC‑‑‑AAAAAAAATaaaaaaaaaaaa                                                                                                                     >  1:3107526/1‑128 (MQ=255)
         ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCAAC‑‑‑TAAAAAATtaaaaaaaaaaaa‑‑a                                                                                                                 >  1:1288826/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATTTAGATCCTAGTGATCTATTTTTATATATTTTTATATTCACAAATAAAAAAAAAACAAT‑‑AAAATTAAAAATTaaaaaaaaaaaaa‑‑aa                                                                                                                >  1:1697743/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAAACAAACCAAAT‑‑AAAAATaaaaaaaaaaaaa‑‑aa                                                                                                                >  2:4114121/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aa                                                                                                                >  1:3933503/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aa                                                                                                                >  2:3744694/1‑128 (MQ=255)
            agtttaatttaAAAACAATAAAAATAATAATTTTTATTATTTTGATACTTTTTTAATATTTTTTTTTTTTTTTAAAATAAAAAATAAAAAAAAAAAAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aaa                                                                                                               <  1:4164608/125‑1 (MQ=255)
            aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAAAAATAAAAAAAAAAAAAAACCC‑‑TTAAAAAATaaaaaaaaaaaaa‑‑aaa                                                                                                               >  1:1014407/1‑128 (MQ=255)
             ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATATTCACAAATAAAAAAAAAACAAACCAC‑‑TAAAAAAATaaaaaaaaaaaaa‑‑aaaa                                                                                                              >  1:2655650/1‑128 (MQ=255)
             ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACCC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aaaa                                                                                                              >  1:3773843/1‑128 (MQ=255)
             ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACACC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aaaa                                                                                                              >  1:4510811/1‑128 (MQ=255)
              tttaattaaAAAAAAATAAAATTAATAATTATTATAAATTATAATCTAGTTATCTATTTTTTTATTTTTTTTTCTTAACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aaaaa                                                                                                             <  2:3600772/127‑1 (MQ=255)
              tttaaataaAATACCATAAAATATAAAATTATTAAAAAGTTATTCCTCGTTTTCTATTTTTTTTTTTGTTTATATTAAAAAATAAAAAAAAAAAAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aaaaa                                                                                                             <  1:1477003/127‑1 (MQ=255)
              gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATATTAACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aaaaa                                                                                                             >  2:470120/1‑128 (MQ=255)
              gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aaaaa                                                                                                             >  2:3982529/1‑128 (MQ=255)
              gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aaaaaa                                                                                                            >  2:3717520/1‑128 (MQ=255)
               ttaattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATATTCACAAATAAAAAAAAAAAAATAAAC‑‑‑TAAAAAATtaaaaaaaaaaaa‑‑aaaaaaa                                                                                                           >  1:42117/1‑128 (MQ=255)
               ttaattaaAAAAACAAAAAAATAATAAATATTATAAATTACATACTCTTGATCTAATTTTTTATTTTTTTAAATTCAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aaaaaa                                                                                                            <  2:1910100/128‑1 (MQ=255)
                taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAT‑‑AAAATTAAAAATTaaaaaaaaaaaaa‑‑aaaaaaa                                                                                                           >  2:17293/1‑128 (MQ=255)
                taattaaAATACCATAAACTTAATAAATCTTATAATGTATATACTCTTGATCTATATTTTTATTTGTTTATCTTAACAAATAAAAAAAAAAAAAAAAAATT‑‑‑AAAAATaaaaaaaaaaaaa‑‑aaaaaaaa                                                                                                          >  2:2720797/1‑128 (MQ=255)
                taattaaAAAAAAATAAAATTAATAATTATTATAATTTAAATCCTCGTGATAAATTTTTTTATTTGTTTATATTAACAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa‑‑aaaaaac                                                                                                           <  2:4493626/128‑2 (MQ=255)
                 aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTATTTATTAGTTTATCATCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaataaaaaaaaaa‑‑aaaaaaaa                                                                                                          >  1:4034247/1‑128 (MQ=255)
                  attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACACAATAAAAATAaaaaaaaaaaaaaaa‑‑aaaaaaa                                                                                                           >  1:2155504/1‑128 (MQ=255)
                     aaaaaaACATAAACTTAATAAAAATTAAAATTTAAAAACACTTCATCTATTTTTTTATTTTTTTAAATTAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTaa                                                                                                      <  2:1353635/128‑1 (MQ=255)
                      aaataaaataAAATTAATAAATATTATAAATTATAAACTAGTTATAAATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTaaa                                                                                                     <  2:1506681/128‑1 (MQ=255)
                      aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAACAATCAC‑‑ATTAAAAATTaaaaaaaaaaaaa‑‑aaaaaaaaaaaa                                                                                                      >  2:526045/1‑124 (MQ=255)
                       aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAAACC‑‑ATTAAAAATTAaaaaaaaaaaaa‑‑aaaaaaaaaaaaaa                                                                                                    >  1:294470/1‑128 (MQ=255)
                       aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTCATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAaaaaaaaaaaaa‑‑aaaaaaaaaaaaaa                                                                                                    >  1:3591128/1‑128 (MQ=255)
                        aaaataTAAAATTAATAATTATTATAATTTATATACTCGTTTTTTATTTTTTTTTTTTTTTATTTTAAAAAATAAAAAAAAAACAATACCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTaaaaa                                                                                                   <  2:3621234/123‑1 (MQ=255)
                        aaaacATAAAAATAATAATTATTTAAATTTTTATCCTCTTGATATATTTTTTTTTTTTTTTATATTCACAAAAAAAAAAAAAAAAACCCCTTAAAAAATAAAAAAAAAAAAAAAA‑‑AAAAAAAACTaaa                                                                                                     <  1:4137291/124‑1 (MQ=255)
                          tataaaaaaTTAATAATTCTTATAATTTATATAATAGTTTTATATTTTTTTTTTTTTTTTTATTAAAAAATAAAAAAAAAAAAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAaaaca                                                                                                 <  2:3283554/123‑1 (MQ=255)
                           aaaaaaaaTTAAAAATTTTTATAATTTTTATCATATTTTTATATTTTTTTTTTTTTTTTTATTCAAAAAAAAAAAAAAAAAAATCCCCTTAAAAATTAAAAAAAAAAAAAA‑‑AAAAAAAACTAAaaaca                                                                                                 <  1:1654674/124‑1 (MQ=255)
                                aaattAATAATTTATAAAATGTATATACTCCTGTAATAATTTTTTTTTTTTTTATATTAAAAAAAAAAAAAAAAACAATCCACTT‑‑‑AAAAATTAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTa                                                                                          <  2:4260752/125‑1 (MQ=255)
                                aaactaataaTTATTATAAATTTATTACTCTTTATTTATTTTTTTATTTTTTTATCTTAAAAAAAAAAAAAAAAAAAAAACCC‑‑TTAAAAAATAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAActct                                                                                           <  2:2380103/124‑1 (MQ=255)
                                aaaataataaATATTATAATGTATATCCACCTGATATATTTTTTTATTTTTTTATATTAACAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTa                                                                                          <  2:4038178/124‑1 (MQ=255)
                                aaCTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAAATAAAAAAAAActaa                                                                                           >  1:3456454/1‑126 (MQ=255)
                                 aattAATAATTATTATAATTTATATAATATTGTTATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAACAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTa                                                                                          <  2:1073690/126‑1 (MQ=255)
                                     tttaatTATTTTAATTTTTATCCTGTTGTTATATTTTTTTTTTTTTTTATATTTCAAAATAAAAAAAAAAAAATCCCACTTAAAAATATAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTAt                                                                                         <  1:2467065/126‑1 (MQ=255)
                                      ataaataatataATTTAGATCTTATTTATATATTTTTTTAATTTTTTAAAATCAAAAAAAAAAAAAAAAAAAATCCCCTTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTc                                                                                      <  2:3782344/120‑1 (MQ=255)
                                          tttatataATTTATATACTCGTTATCTTATTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAAt                                                                               <  2:4139324/124‑1 (MQ=255)
                                               ataatttttatacTCGTTATTTATTTTTTTATTTTTTTATCATAAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTccc                                                                           <  1:3868079/116‑1 (MQ=255)
                                                      ttaTCCTCGTGTTATTTTTTTTTATTTTTTTAAATTAACAAAAAAAAAAAAAAAAAAACCCATAAAAAATAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatta                                                                      <  2:579645/126‑1 (MQ=255)
                                                      atataatCGTTATATATTTTTTTATTTTTTTAAATTAAAAAATAAAAAAAAAAAAATACCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                                     <  2:1141826/122‑1 (MQ=255)
                                                        atcagatttatttATTTTTTTATATTTTTATAATAAAAAAAAAAAAAAAAAAAAACCCCATAAAAAATAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATtata                                                                    <  2:2607028/116‑1 (MQ=255)
                                                        ataatatttatttatttttttattttttTTTATTAAAAAAAAAAAAAAAAAAAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAc                                                                   <  2:2479208/116‑1 (MQ=255)
                                                         tcaacgtttaatATTTTTTTATTTGTTTATTATAAAAAAAAAAAAAAAAAAAAACCC‑‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                                 <  2:3907307/117‑1 (MQ=255)
                                                          ctctttttttatttttttaATTTTTTTATATTAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                                 <  1:3625606/116‑1 (MQ=255)
                                                          cgtcggtttatttttttttATTTGTTTATATTAACAAATAAAAAAAAAACAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                                 <  1:581766/116‑1 (MQ=255)
                                                          catCGTTATATATTTTTTTTTTTTTTTATAATAAAAAATAAAAAAAAAAAAATCCCC‑‑TTAAAAAATAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                                <  2:883503/126‑1 (MQ=255)
                                                          atttttatttattttttttattttttttatTAAAAAAAAAAAAAAAAAAAATACCCTTAAAAATTAAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                                  <  2:4358255/116‑1 (MQ=255)
                                                          aatatttatatATTTTTTTTTTTTTTTATATTTAAAAATAAAAAAAAAAAAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                                 <  2:15791/118‑1 (MQ=255)
                                                            cgtttatatATTTTTTTATATTTATATCTAAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAAAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACat                                                              <  1:983545/120‑1 (MQ=255)
                                                             tttttttattttttttATTTTTTATATTAAAAAAAAAAAAAAAAAAAATACCATTTAAAATAAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACat                                                              <  2:1451069/119‑1 (MQ=255)
                                                              tttttaaatttttttatttttttATATTAAAAAAAAAAAAAAAAAAAAAACCCTTAAAAAATAAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcaca                                                               <  2:251996/121‑1 (MQ=255)
                                                               ttatatatttttttattttttTATATTAAAAAATAAAAAAAAAAAAATACCCTAAAAAATTAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                             <  2:4202325/123‑1 (MQ=255)
                                                               cttatttattttttATATTTTATATATTCTAAAAAAAAAAAAAAAAAAAACCCATT‑‑AAAAATAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                             <  1:460843/120‑1 (MQ=255)
                                                                 ttttATTTTTTTTTTTTTTTATATTAAAAAATAAAAAAAAAAAAATCACCTTAAAAATTAAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                            <  2:2814305/125‑1 (MQ=255)
                                                                  tatatttttttattttttTAAATTAAAAAAAAAAAAAAAAAAAATACCATTAAAAATTAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                                          <  2:1785856/126‑1 (MQ=255)
                                                                    atttttttttttttttTATATTAAAAAAATAAAAAAAAAAAAAACCATTAAAAATATAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttattt                                                       <  1:380523/126‑1 (MQ=255)
                                                                    attttttttATTTTTTATATTAAAAAAAAAAAAAAAAAAAAAACCCTT‑‑‑AAAAATTAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                                     <  2:81244/126‑1 (MQ=255)
                                                                     tttttttttttttttttttttAAAAAATAAAAAAAAAACAATCCC‑‑ATTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                                      <  1:3556797/127‑1 (MQ=255)
                                                                     ttttttttATTTTTTATATTAAAAAAAAAAAAAAAAAAAATTCCCTT‑‑‑AAAAATTAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                                    <  2:41814/127‑1 (MQ=255)
                                                                      ttttttttttTGTTTTTAGTAAAAAATAATAAATAAACATATCCC‑‑TTAAAATTTTAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                                     <  2:2315927/128‑1 (MQ=255)
                                                                       ttttttatttttttatTTAAAAAAATAAAAAAAAAAAAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                                    <  1:3486265/128‑1 (MQ=255)
                                                                        tttttATTTTTTTATCATCACAAAAAAAAAAAAAAACAAACCCC‑‑TTAAAAAATAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                                    <  1:4248648/128‑1 (MQ=255)
                                                                        tttttATTTTTTTATATTAAAAAAAAAAAAAAAAAAAA‑‑AACAATTAAAAATAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                                   <  1:3414657/128‑1 (MQ=255)
                                                                         ttttttttttttATATTTAAAAAAAAAAAAAAAAAAAAAACATTAAAAATTAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                                   <  2:454185/128‑1 (MQ=255)
                                                                          tttATTTTTTAATTTAAAAAAAAAAAAAAAAAAAAAAACCC‑‑‑TAAAAAATAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                                               <  1:1739004/128‑1 (MQ=255)
                                                                            tATTTTTTTATATTAAAAAATAAAAAAAAAAAAATACC‑‑TTTAAAAATTAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                              <  2:3452202/128‑1 (MQ=255)
                                                                             tttttttTATATTAACAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAAATAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                              <  1:4361674/127‑1 (MQ=255)
                                                                             aatatttttATCTTAAAAAAAAAAAAAAAAAAAATCCCATT‑‑‑AAAAATTAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                              <  2:406753/122‑1 (MQ=255)
                                                                              aattGTTTATTTTAACAAATAAAAAAAAAAAAATTCC‑‑ATTAAATATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                              <  2:212776/126‑1 (MQ=255)
                                                                               aatttttATATTAAAAAAAAAAAAAAAAAAAATACCC‑‑TTAAAAAATAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAg                                             <  2:319456/124‑1 (MQ=255)
                                                                                ttGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAAATAAAAAAAAACTATATTTATTAAAATTACCTTATATAAAAAATTTTTTTTTTAaaaaaata                                            >  2:2430266/1‑126 (MQ=255)
                                                                                       taaatataaaaaaaaaaaaaaaaaaaaaCCCATTAAAAAATAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                                        <  1:764708/120‑1 (MQ=255)
                                                                                       atattaaaaaaTAAAAAAAAAACAAACCCCTTAAAAATTAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTg                                       <  2:2010020/120‑1 (MQ=255)
                                                                                        atttttaaaaTAAAAAAAAAAAAAATTCCAATAAAAATTAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTg                                       <  1:518498/121‑1 (MQ=255)
                                                                                          ttaaaaaaTAAAAAAAAAACAATAACATT‑‑‑AAAAATTAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                                  <  2:2228123/123‑1 (MQ=255)
                                                                                            taaaaaaaaaaaaaaaaaaaaACAATTAAAAATAAAAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACat                                   <  2:1613993/125‑1 (MQ=255)
                                                                                            aaaaaaaaaaaaaaaaaaaaaACCC‑‑TTAAAAATTAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataa                                 <  1:3999052/125‑1 (MQ=255)
                                                                                             aaaaaaaaaaaaaaaaaaATACCCATT‑‑AAAAAT‑‑‑AAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatata                             <  1:1076067/126‑1 (MQ=255)
                                                                                                    aaaaaaaaaaCAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAA‑‑AAAAAAAATAAAAAAAAAATATATTTATAAAAATTAAAAATTAAACAACATTTTATATATAAAATAATATATAGAAATAaaataaatat                        >  2:3525778/1‑126 (MQ=255)
                                                                                                              aaaaatacccttAAAAAATAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatata                  <  2:3468078/118‑1 (MQ=255)
                                                                                                              aaaaataaccttaaaaattaaaaaAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatata                  <  1:3745101/118‑1 (MQ=255)
                                                                                                               aaataaactaataaaaaaaaaaaaaaaaaaa‑‑aaaaaaaACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatatat                 <  2:1204963/116‑1 (MQ=255)
                                                                                                               aaaaatacaattaaaaattaaaaaAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatatat                 <  1:2354556/116‑1 (MQ=255)
                                                                                                               aaaaaaacccttaaaaattaaaaaAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatatat                 <  1:2310568/116‑1 (MQ=255)
                                                                                                                 aatacccttaaaaattaaaaaAAAAAAAA‑‑AAAAAAAACTAAAAAAAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatatatat               <  1:3510617/121‑1 (MQ=255)
                                                                                                                 aaataaacttaaaaattaaaaaAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatatatat               <  2:4418863/118‑1 (MQ=255)
                                                                                                                  atacaattAAAAAATAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATataa             <  2:1264015/122‑1 (MQ=255)
                                                                                                                  ataaaattaaaaattaaaaaAAAAAAAAATAAAAAAAACTAAAAACATACTCTATTTCTTAAAATTCACTATTATAACACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatatata                <  2:1641002/122‑1 (MQ=255)
                                                                                                                   attcaaataaaaattaaaaaAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCACTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATataa             <  2:3823536/120‑1 (MQ=255)
                                                                                                                     ccattaaaaattaaaaaAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATataat            <  1:4100015/125‑1 (MQ=255)
                                                                                                                        ttaaaaattaaaaaAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACt         <  2:3861709/128‑1 (MQ=255)
                                                                                                                             aaaaaaaaaaaaaaaaa‑‑aaaaaaaACTAAAAACAAACTCTATTTCATAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTATATTg   <  1:4479035/128‑1 (MQ=255)
                                                                                                                               aataaaaaaaaaataa‑‑aaaaaaAACTGAAATCAAACTCTATTTCTTAAAATTCAATATTATAACACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTATATTGc  <  2:2923851/128‑1 (MQ=255)
                                                                                                                                |                                                                                                                                 
ATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAA‑‑AAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTATATTGC  >  CP016816/202370‑202620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: