Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 20,701 +A coding (286/1920 nt) JCVSYN2_00070 → tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681620,6991.A93.9% 3881.5 / 215.6 1371coding (284/1920 nt)JCVSYN2_00070tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base . (41/41);  new base A (672/595);  total (726/645)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1454 total aligned reads displayed.

GGAATTACAATTCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGACCAAAATGGAATTG  >  CP016816/20572‑20821
                                                                                                                                 |                                                                                                                            
ggACTTACAATTCAGACAGGAAAAACCTCTGTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGc                                                                                                                               <  1:1221250/128‑1 (MQ=255)
  aaTTACAATTCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACCTCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGGATATCGTTCATTATCAGTGTTAGATGGGGCaa                                                                                                                           >  2:2482630/1‑128 (MQ=255)
   aTTACAATACAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACAAATGGATTTCGTAGCAGAAGTAAAACGAACAGTATCAGTTTTAGATCGGTCaca                                                                                                                           >  2:1380474/1‑126 (MQ=255)
    ttACAATTCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCaaat                                                                                                                         <  2:1291031/128‑3 (MQ=255)
       cAATTCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACAAGCCAGGACAAATCTATTTCTTAGCAGAAGTATATCGTTCATTATCAGATTTAGATGGGGCaaattct                                                                                                                      >  2:89723/1‑123 (MQ=255)
           tcagtcagGAATCACCTCTTTTCCGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTg                                                                                                                  <  1:4123926/128‑1 (MQ=255)
           tCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTACATTATCAGTTTTAGATGGCGCAAATTCTAcag                                                                                                                  >  2:801639/1‑126 (MQ=255)
           tCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGACAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGGTGTAGATGGTGCAAATTCTACTg                                                                                                                  >  2:3115324/1‑128 (MQ=255)
             aGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGAt                                                                                                                >  2:2694182/1‑128 (MQ=255)
             aGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGAt                                                                                                                >  2:381509/1‑128 (MQ=255)
             aGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAACAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGAt                                                                                                                >  2:450458/1‑128 (MQ=255)
              gACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGAtt                                                                                                               <  1:250610/128‑1 (MQ=255)
               aCAGTAATAACGTCTTTTCAGTGGTAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATCGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGAttt                                                                                                              <  1:814310/128‑1 (MQ=255)
                  ggAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGTGGCAAATTCTACTGATTTCTg                                                                                                           >  2:2560826/1‑128 (MQ=255)
                   gAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGc                                                                                                          >  2:4402880/1‑128 (MQ=255)
                      tAACCTCTATTCAGTGGGAAAATCCGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCaaa                                                                                                       <  1:1399520/128‑1 (MQ=255)
                       aaCCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCaaaa                                                                                                      >  1:1500342/1‑128 (MQ=255)
                          ctctTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCAGTATCAGTTTTAGATGGGGCAAAGTCTACTGATTTCTGCAAAAGAt                                                                                                   >  1:4496654/1‑128 (MQ=255)
                          ctctTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATAAGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTATAGATGGGGCTAATTCTACTGATTTCTGCAAAAGAt                                                                                                   <  2:2773720/128‑1 (MQ=255)
                           tctTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATg                                                                                                  >  1:4373608/1‑128 (MQ=255)
                             ttttCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGc                                                                                                >  1:3095658/1‑128 (MQ=255)
                             ttttCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTGTTAGCTGGGGCAAATTCTACTGATTTCTGCAAAAGAGGGc                                                                                                >  1:2342238/1‑128 (MQ=255)
                               ttCAGTGGGAAAATTCTATGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGt                                                                                              <  2:1187690/128‑1 (MQ=255)
                               ttCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATTTCGTTCATTATCAGTTTTAGCTGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGt                                                                                              >  1:2052024/1‑128 (MQ=255)
                               ttCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGt                                                                                              <  2:4204975/128‑1 (MQ=255)
                               ttCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGGTCATTATCAGTTTTAGATGGGTCATATTCTACTGATTTCTGCAAAAGATGGctg                                                                                              >  1:1142684/1‑126 (MQ=255)
                                tccgttggAACATACGAAGGTGACCATCCTAGACACTACAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTa                                                                                             <  1:2903069/122‑1 (MQ=255)
                                tCAGTGGGAAATTACGAATTTGAACATCATATACTCGCCAGGCCATATGGTTTTCTTAGCAGACGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTa                                                                                             <  1:1934525/128‑1 (MQ=255)
                                 cAGTGGGAAAATACGATGGTGAACATCTTCGACATGCCAGTACATATGGATTTCTTAGCATAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTAc                                                                                            <  2:2057360/128‑1 (MQ=255)
                                 cAGTGGGAAAATACGAAGGTGAACATCCTAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTAc                                                                                            <  1:429471/128‑1 (MQ=255)
                                 cAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGAt                                                                                                                <  1:3665827/108‑1 (MQ=255)
                                 cAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGAt                                                                                                                >  2:3665827/1‑108 (MQ=255)
                                  aGTGGGAAAATAAGAAGGTGAACATCAGAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACa                                                                                           <  1:937948/128‑1 (MQ=255)
                                        aaaaTACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGcaca                                                                                     >  1:109209/1‑128 (MQ=255)
                                        aaaaTACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGcaca                                                                                     >  2:4281742/1‑128 (MQ=255)
                                          catccGAATGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACaaa                                                                                   <  1:3409171/124‑1 (MQ=255)
                                           aTACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTTTCAGTTTTAGAATGGGAAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGAACAAAc                                                                                  >  2:716812/1‑128 (MQ=255)
                                                aaGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGta                                                                             <  2:335457/128‑1 (MQ=255)
                                                  ggTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGtata                                                                           >  2:4614069/1‑128 (MQ=255)
                                                  ggTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATAAGTTTTAGATGGGTCAAATTCTACTGATTTCTTCAAAAGATGGCTTAAAATCACAAACTCGtata                                                                           >  2:507701/1‑128 (MQ=255)
                                                    tgtaCATCTAAGACAAGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTAtatt                                                                         <  1:3595753/125‑1 (MQ=255)
                                                    tGAACATCATAGCCACGCCCGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCTATTTCTACTGCTTTCTGCAAAAGATGGCGTACAAGCACACTCTCGAAtatt                                                                         >  1:2126677/1‑128 (MQ=255)
                                                    tGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGAGTTCTGCTAAAGAAGGCGTTCAAGCACAAACTCGGAtatt                                                                         >  1:4178664/1‑128 (MQ=255)
                                                     gAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATAGCGTTCATTATCAGTGTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTagataa                                                                        >  1:3300973/1‑126 (MQ=255)
                                                       aCATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATattatt                                                                      >  2:3722200/1‑128 (MQ=255)
                                                             tAGACTAGCCAGGACATCTGGATTTCTTAGCAGTAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGc                                                                <  1:1764214/128‑1 (MQ=255)
                                                             tAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGc                                                                >  1:4070730/1‑128 (MQ=255)
                                                             tAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGc                                                                >  1:1591960/1‑128 (MQ=255)
                                                              agactcgggaGGACATAAGGATTTCTTAGCTGAAGTATATCGTTCATTATCAGTTATTGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTTTTTAATGCa                                                               <  2:1755039/119‑1 (MQ=255)
                                                              aGACAGTCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGTCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCa                                                               <  1:3154502/128‑1 (MQ=255)
                                                               gACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACTCGCACCAACTCGGAAAGTAGTTCAGGCAc                                                              >  2:2369144/1‑128 (MQ=255)
                                                                 cacGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCATAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGctcta                                                            >  1:3803585/1‑127 (MQ=255)
                                                                   cGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGAtt                                                                                                               >  1:3869622/1‑75 (MQ=255)
                                                                   cGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAg                                                          <  1:3240015/128‑1 (MQ=255)
                                                                   cGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATAAGTTTTAGATGTGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACACGCACAAACTCGTATAGTATTTCATGCACGTAg                                                          >  1:2716966/1‑128 (MQ=255)
                                                                    gCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAgg                                                         <  2:4131781/128‑1 (MQ=255)
                                                                     cccggACATTGGGATTGCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGa                                                        <  1:3977443/125‑1 (MQ=255)
                                                                        ggACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGCaaaa                                                     >  2:582985/1‑128 (MQ=255)
                                                                          aCATATGGATTTCTTAGCAGAAGTATCTCGTTCTATATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATg                                                   <  1:3476652/128‑1 (MQ=255)
                                                                          aCATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGCTGGCGTACAAGCACAAACTCGAAGATTATATCATGCACTTAGGAAAAta                                                   >  2:3195355/1‑127 (MQ=255)
                                                                             tatGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTACAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAg                                                          >  1:710898/1‑118 (MQ=255)
                                                                              atGGATTTCTTCGCAGAAGTATATCGTTCATTATCCGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAATATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGa                                               <  2:1871979/128‑1 (MQ=255)
                                                                              atGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGa                                               <  1:2619233/128‑1 (MQ=255)
                                                                                   tttCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCTATTTCTACTGATTTCTGCTAAAGATGGCGAACCTGCCCAACCTCGTCTAAAATTTCTAGCAAATACGAAAATGAGGATTccc                                          >  1:3016597/1‑128 (MQ=255)
                                                                                   tttCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTccc                                          <  2:4350006/128‑1 (MQ=255)
                                                                                       ttAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTccc                                          >  2:672686/1‑124 (MQ=255)
                                                                                        tAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAAt                                     <  2:628916/128‑1 (MQ=255)
                                                                                         aGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGTCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATc                                    <  1:3722561/128‑1 (MQ=255)
                                                                                          gCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCt                                   >  1:278845/1‑128 (MQ=255)
                                                                                            aGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCttt                                 >  2:2630771/1‑128 (MQ=255)
                                                                                             gAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGAtt                                             <  2:4057400/115‑1 (MQ=255)
                                                                                             gAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCtttt                                <  2:2843167/128‑1 (MQ=255)
                                                                                             gAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCtttt                                <  2:1116538/128‑1 (MQ=255)
                                                                                             gAAGTATATCGTTCATTATCAGTGTTAGATGGGGCTAATTCGACAGATTTCTGCAAACGCTGGCGAACAAGCACAAACTCGTATTATATTTCCTGCACGTAGGAAAATGGGGCTGCCCACActctctg                                >  1:2402865/1‑125 (MQ=255)
                                                                                               tgtagagagTTCATTATCAGTGTTAGATGGGGCTAATTCTACTGATTTCTGCAAAAGATGGCGTACACGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCtttttt                              <  1:1298452/120‑1 (MQ=255)
                                                                                               aGTATCTCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCtttttt                              <  2:1028601/128‑1 (MQ=255)
                                                                                               aGTATATCGTTCATTATCAGTTTTAGATGGTGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCtttttt                              <  2:167117/128‑1 (MQ=255)
                                                                                               aGTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCttattg                              <  1:1009989/128‑2 (MQ=255)
                                                                                                gTATTTCTTTTATTATCAGTTTTAGATGGGGCAATTTATACTGATTTCTGCATAAGATTGCGTACAAGGACAAATTCGTATATTATTTGATGCACTTAGGAAAATGGGGATTCCCACAATCttttttt                             <  1:2174102/128‑2 (MQ=255)
                                                                                                gTATATCGTTGATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTa                             <  2:2174029/128‑1 (MQ=255)
                                                                                                gTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTa                             >  2:3258377/1‑128 (MQ=255)
                                                                                                gTATATCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCACACAATCTTTTTTa                             >  2:2982690/1‑128 (MQ=255)
                                                                                                 tataTCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGCATACCCACAAACGTTTGTAt                            >  2:2937936/1‑128 (MQ=255)
                                                                                                  atatCGTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATc                           >  2:1981000/1‑128 (MQ=255)
                                                                                                     tCGTTCATTATCAGTTTTAGATTGGGCAAATTCTTCTGATTACTGCAAAAGATGGCGTACAAGCACAACCTCGTATATTATATCATGAACTAAGGAAAATGGGGATTCCCACTATCTTTTTTATCAAt                        >  2:2273345/1‑128 (MQ=255)
                                                                                                       gTTCATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCTCTTAGGAAAATGGGGATACACACAATCTTTATTATCAATaa                      >  2:3613837/1‑128 (MQ=255)
                                                                                                          cATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGAt                   >  2:3964360/1‑128 (MQ=255)
                                                                                                          cATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGAt                   >  2:3431344/1‑128 (MQ=255)
                                                                                                          cATTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACGTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGAt                   >  2:3903874/1‑128 (MQ=255)
                                                                                                           aTTATCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGAtt                  >  1:462614/1‑128 (MQ=255)
                                                                                                              aTCAGTTTTAGATGTGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGAc               <  2:352940/128‑1 (MQ=255)
                                                                                                              aTCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGAc               <  2:1924290/128‑1 (MQ=255)
                                                                                                              aTCAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGAc               <  1:1855045/128‑1 (MQ=255)
                                                                                                                cAGTTTTAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGACCa             <  2:1527710/128‑1 (MQ=255)
                                                                                                                 aGTTTAAGATGGGGCAAAATCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGACCaa            <  1:1660397/128‑1 (MQ=255)
                                                                                                                   ttttAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGACCaaaa          <  1:2564986/128‑1 (MQ=255)
                                                                                                                      tAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGACCAAAATgg       >  1:654799/1‑128 (MQ=255)
                                                                                                                      tAGATGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCAACAATCTTTTTTATCAATAAGATTGACCAAAATgg       <  1:51953/128‑1 (MQ=255)
                                                                                                                           tGGGGCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGACCAAAATGGAAtt   >  2:1826405/1‑128 (MQ=255)
                                                                                                                            ggggCAAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGTAAAATGGGGATTCCCACAAACATATTTATCAATAAGATTGACCAAAATGGAATTg  >  2:1598495/1‑128 (MQ=255)
                                                                                                                                 |                                                                                                                            
GGAATTACAATTCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGACCAAAATGGAATTG  >  CP016816/20572‑20821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: