Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 19,719 (A)9→8 intergenic (‑440/‑697) JCVSYN2_00065 ← / → JCVSYN2_00070 hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681619,7110A.98.4% 7272.7 / 116.5 1765intergenic (‑432/‑705)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base A (16/12);  new base . (825/889);  total (848/917)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1898 total aligned reads displayed.

TTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAGTAAAATGTAAAAT  >  CP016816/19584‑19834
                                                                                                                               |                                                                                                                           
   cacGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTATAAGAGTTAAGGGATGATGaaaa                                                                                                                          >  2:2820191/1‑128 (MQ=255)
   cacGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGaaaa                                                                                                                          >  2:2709813/1‑128 (MQ=255)
    acGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGaaaaa                                                                                                                         <  1:2241461/128‑1 (MQ=255)
         ccTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAATGTAGAAGAGTTACGGAATGAAGAAAAaataat                                                                                                                    >  2:2070731/1‑128 (MQ=255)
           tCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGTTTTTATAAAGTAAATAAAGAAGGTAGAAGAGTTACTGAATGATGaaaaaacataaa                                                                                                                  >  2:948444/1‑122 (MQ=255)
             gTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGCAGAAAAAAAATAAACa                                                                                                               >  1:4415394/1‑128 (MQ=255)
             gTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACa                                                                                                               >  2:4637268/1‑128 (MQ=255)
             gTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACa                                                                                                               >  2:2149786/1‑128 (MQ=255)
             gTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGAAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACa                                                                                                               <  2:1338629/128‑1 (MQ=255)
               aTCTTTTAATGATGGAATAATTGGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACaaa                                                                                                             <  1:802702/128‑1 (MQ=255)
                 cTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAgg                                                                                                           <  2:2313352/128‑1 (MQ=255)
                  ttttAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAgtt                                                                                                          >  2:1493515/1‑126 (MQ=255)
                  ttttAATGATCGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGt                                                                                                          <  1:3178703/128‑1 (MQ=255)
                    ttAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAATAAGGTAGAAGAGTTAAGGAATGAAGAAAAAAAATAAACaaattttt                                                                                                        >  2:3386266/1‑123 (MQ=255)
                    ttAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGttt                                                                                                        >  1:474698/1‑128 (MQ=255)
                    ttAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTATAGAAAGTAAATAAAGAAGGTAGATGAGTTACTGAATGAAGAAAAAAAATAAACAAAGGttt                                                                                                        >  2:2252673/1‑128 (MQ=255)
                       atgatgGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTaaa                                                                                                     >  1:1621274/1‑128 (MQ=255)
                        tgctgGAATAATTTGGGAATTTACTCAGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTaaaa                                                                                                    <  1:552568/125‑1 (MQ=255)
                          atgGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTaaaaaa                                                                                                  <  1:3959530/128‑1 (MQ=255)
                           tgGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAATAAATAAACAAAGCTTTAAAAAAt                                                                                                 >  1:1212763/1‑128 (MQ=255)
                            ggAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAAtt                                                                                                <  2:4062050/128‑1 (MQ=255)
                             gAATACTTTGGGAATATACTCTGGGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAAttt                                                                                               <  2:861859/128‑1 (MQ=255)
                             gAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACa                                                                                                               <  2:755088/112‑1 (MQ=255)
                             gAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTaaaaaa                                                                                                  >  2:3172495/1‑125 (MQ=255)
                              aataatTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATATAAAAAGGTTTAAAAAATTTc                                                                                              >  1:3101850/1‑128 (MQ=255)
                               ataatTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTca                                                                                             <  2:1425787/128‑1 (MQ=255)
                                taatTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTcaa                                                                                            <  2:1765535/128‑1 (MQ=255)
                                taatTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTAACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTcaa                                                                                            >  1:2884186/1‑128 (MQ=255)
                                  atTTGTGAATTTACTCTGTTTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTcaaca                                                                                          <  2:4177512/128‑1 (MQ=255)
                                  atTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTcaaca                                                                                          <  2:4442869/128‑1 (MQ=255)
                                  agttGTGAATTTTCTCTGTCTGTATTTATTTTTATGTGTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTATAAAAAATTTcaaca                                                                                          <  1:1115929/126‑1 (MQ=255)
                                   tttGTGAATTTCCTCTGTGTTTATTTATTTTTATGATTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTcaacaa                                                                                         <  1:1974597/128‑1 (MQ=255)
                                   tttGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTcaacaa                                                                                         <  2:2529349/128‑1 (MQ=255)
                                    ttGGGAATTAACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACaaa                                                                                        <  1:4574397/128‑1 (MQ=255)
                                     tGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACaaaa                                                                                       <  1:1845624/128‑1 (MQ=255)
                                      gggAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACaaaa                                                                                       >  1:3340946/1‑128 (MQ=255)
                                         aaattACTCTTTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCg                                                                                   <  2:3973054/125‑1 (MQ=255)
                                          aTTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGt                                                                                  >  1:3486325/1‑128 (MQ=255)
                                           tttACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTa                                                                                 >  2:3828117/1‑128 (MQ=255)
                                            ttACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAATAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTAc                                                                                >  1:3401223/1‑128 (MQ=255)
                                            ttACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAATAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTAc                                                                                >  2:1980772/1‑128 (MQ=255)
                                            ttACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTAc                                                                                <  2:4326452/128‑1 (MQ=255)
                                             tACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTAcg                                                                               >  1:1059657/1‑127 (MQ=255)
                                             tACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACt                                                                               <  1:3649028/128‑1 (MQ=255)
                                             tACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACt                                                                               <  2:1578459/128‑1 (MQ=255)
                                               ctctGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACttt                                                                             <  1:151565/128‑1 (MQ=255)
                                                tctGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTa                                                                            <  1:1377604/128‑1 (MQ=255)
                                                tctGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTa                                                                            <  1:245210/128‑1 (MQ=255)
                                                 ctGTGTTGATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTAc                                                                           <  2:191403/128‑1 (MQ=255)
                                                   gtgtTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACat                                                                         <  2:3111732/128‑1 (MQ=255)
                                                    tgtTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTTCGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAATAGCGTACTATACata                                                                        >  1:1670611/1‑128 (MQ=255)
                                                        tatttattTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACatatata                                                                    >  2:3577751/1‑128 (MQ=255)
                                                          tttatttTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGATTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATAtatt                                                                  >  2:3885052/1‑128 (MQ=255)
                                                              tttttATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATAtatttatt                                                              <  1:4151093/128‑1 (MQ=255)
                                                                tttATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAg                                                            <  1:4073757/128‑1 (MQ=255)
                                                                 ttATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAg                                                            <  1:4334939/128‑1 (MQ=255)
                                                                     gTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTGCGGAATTAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAg                                                       <  2:1707486/128‑1 (MQ=255)
                                                                       tttGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAATTAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGaa                                                     <  1:3719836/128‑1 (MQ=255)
                                                                        ttGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGaaa                                                    >  2:1256265/1‑128 (MQ=255)
                                                                         tGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACatat                                                                       <  2:3039906/108‑1 (MQ=255)
                                                                         tGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGaaaa                                                   <  1:3805002/128‑1 (MQ=255)
                                                                         tGTATATGGATTTTAGAAAGTAAATAACGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGaaaa                                                   <  1:913322/128‑1 (MQ=255)
                                                                              ttGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAg                                                       <  2:3265650/119‑1 (MQ=255)
                                                                              ttGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGa                                              <  2:3732493/128‑1 (MQ=255)
                                                                               tGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACTGAATTAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCTAACTTTACATATATATTTATTAGACAAGAAAAGCAGAt                                             >  2:1160463/1‑128 (MQ=255)
                                                                                 gATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTa                                           <  1:2812444/128‑1 (MQ=255)
                                                                                 gATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGAGATTaa                                          <  2:333082/128‑1 (MQ=255)
                                                                                    tttAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGTTTTAAAAAATATCACGAAAAAGCGTACTTTCCATATATATTGAGTAGACCAGACTAGCAGATTaatt                                        >  2:290072/1‑126 (MQ=255)
                                                                                    tttAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATTTATATTTAGTTGACTAGAATAGCTGATTaata                                        >  1:2131101/1‑126 (MQ=255)
                                                                                    tttAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAAt                                        <  1:1276243/128‑1 (MQ=255)
                                                                                      tAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAg                                      <  1:3252196/128‑1 (MQ=255)
                                                                                      tAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAg                                      <  1:1916156/128‑1 (MQ=255)
                                                                                      tAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAg                                      >  2:4544614/1‑128 (MQ=255)
                                                                                        gAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGat                                    <  2:3502376/128‑1 (MQ=255)
                                                                                        gAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGat                                    <  2:546241/128‑1 (MQ=255)
                                                                                        gAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGat                                    <  1:251496/128‑1 (MQ=255)
                                                                                        gAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGat                                    <  2:164897/128‑1 (MQ=255)
                                                                                            gTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTTTTAGACAAGAAAAGCAGATTAAATAGATATAc                                >  1:635491/1‑128 (MQ=255)
                                                                                            gTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAg                                                  >  1:2365376/1‑110 (MQ=255)
                                                                                              aaataaaGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCATCAACAAGCGTCCTGGACCTATTAAGTAATTATCCAAGAAACGCACATTAAATAGatattcct                              >  1:2992746/1‑124 (MQ=255)
                                                                                                ataaaGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTc                            <  1:3868552/128‑1 (MQ=255)
                                                                                                 taaaGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGAAAAGAAAACCAGATTAATTAGAAATAAATTCg                           >  1:2622915/1‑128 (MQ=255)
                                                                                                  taagaagTTAGAAGAGTTACTGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGa                          <  2:1066201/127‑1 (MQ=255)
                                                                                                  atagaagCTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAATGGTTTAAATAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGa                          <  1:719281/126‑1 (MQ=255)
                                                                                                  aaaGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGa                          <  2:239482/128‑1 (MQ=255)
                                                                                                   aagaagGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTATACAAGAAAAGCAGATTAAATAGATATACATTCGAt                         >  2:3681753/1‑128 (MQ=255)
                                                                                                    agaagTTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAATAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGAtt                        <  2:64276/128‑1 (MQ=255)
                                                                                                    agaagGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAATGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGCTTAATGAGAGAGACCTTCgtgt                        >  1:993902/1‑125 (MQ=255)
                                                                                                     gaagGTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTa                       <  1:2732422/128‑1 (MQ=255)
                                                                                                         gTAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACTTTCGATTAACGa                   >  2:2451271/1‑128 (MQ=255)
                                                                                                          tAGAAGAGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGa                   <  1:1761594/127‑1 (MQ=255)
                                                                                                           agaagaGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACTTTCGATTAGCGaga                 >  1:1001628/1‑126 (MQ=255)
                                                                                                            gaagaGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATaa                <  1:1446733/128‑1 (MQ=255)
                                                                                                             aagaGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAACAAGCGTACTTTACATATATATTTATTAGACAAGAAAATCAGATTAATTAGATATACATTCGATGACCGATTAg               >  1:2061254/1‑128 (MQ=255)
                                                                                                             aagaGTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAg               <  2:1813667/128‑1 (MQ=255)
                                                                                                               gagTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAgta             >  1:4224821/1‑128 (MQ=255)
                                                                                                                agTTACGGAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAgtaa            <  1:4023659/128‑1 (MQ=255)
                                                                                                                      ggAATGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATaa                >  1:3583233/1‑118 (MQ=255)
                                                                                                                        aaTGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAgtaaaatgtaaa    <  2:4225321/128‑1 (MQ=255)
                                                                                                                          tGAAGAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAgtaaaatgtaaaat  <  2:2608731/128‑1 (MQ=255)
                                                                                                                               |                                                                                                                           
TTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAGTAAAATGTAAAAT  >  CP016816/19584‑19834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: