BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.5%	3825.4 / NA	1228	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (3/3); new base T (621/597); total (627/601)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	98.5%	3669.6 / ‑2.2	1228	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (2/17); new base T (624/584); total (627/601)
Fisher's exact test for biased strand distribution p-value = 2.91e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1281 total aligned reads displayed.

TAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACG  >  CP016816/420035‑420279

||

      cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                    <  1:1972072/128‑1 (MQ=255)

       attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                   <  1:824075/128‑1 (MQ=255)

       attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                   <  2:1491019/128‑1 (MQ=255)

        ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTTATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                  >  1:581952/1‑128 (MQ=255)

        ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                  <  1:1786420/128‑1 (MQ=255)

            tACTTTTAGTATCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAATAAAATAATAATTTTATTAGTTTATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                              <  2:646579/128‑1 (MQ=255)

             aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                             >  2:2405071/1‑128 (MQ=255)

             aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                             <  1:2391896/128‑1 (MQ=255)

              cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                            <  2:1059520/128‑1 (MQ=255)

              cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                            >  2:2479575/1‑128 (MQ=255)

                 ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                         >  2:881714/1‑128 (MQ=255)

                 ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                         <  1:1597440/128‑1 (MQ=255)

                 ttAGTCTCATAAATCTAAGTTACTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                         >  2:602289/1‑128 (MQ=255)

                     tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTATTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                     >  2:803500/1‑128 (MQ=255)

                      ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                    >  1:2031298/1‑128 (MQ=255)

                         aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                 <  2:468087/128‑1 (MQ=255)

                          tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                <  2:932878/128‑1 (MQ=255)

                            aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTATTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAAtttt                                                                                              >  2:1164010/1‑126 (MQ=255)

                            aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                              <  2:21957/128‑1 (MQ=255)

                            aaTCTAAGTTTATAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                              <  2:3308909/128‑1 (MQ=255)

                             aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTATTCAAATTCtt                                                                                             >  2:1295336/1‑128 (MQ=255)

                                tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                          <  1:1669765/128‑1 (MQ=255)

                                 aaGTTTCTATAATTATATTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATGACTGAATTATTTTTCAAATTGTTAAat                                                                                         <  2:2883878/128‑1 (MQ=255)

                                 aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                         <  2:3255210/128‑1 (MQ=255)

                                  aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                        <  1:139315/128‑1 (MQ=255)

                                   gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                       >  1:1866944/1‑128 (MQ=255)

                                   gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                       >  1:647445/1‑128 (MQ=255)

                                   gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAataa                                                                                       >  2:1640620/1‑126 (MQ=255)

                                     ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                     <  1:2887705/128‑1 (MQ=255)

                                     ttCTAAAATTATTTTATAATTTAAAAAAATAATAATTTTATTATTTGATATTTTAGAAAAATAGTAGAAATTTTTTAAAAATTTTTTAATTTTAATAAATGATTTATTTTAAAAATTGTAAAataatt                                                                                     >  1:1039647/1‑128 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                               <  1:2195061/116‑1 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                   >  2:1917596/1‑128 (MQ=255)

                                        tAAAATTATTTTATCATTTAAAAAATTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                  <  1:975585/128‑1 (MQ=255)

                                        tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                  <  1:3193608/128‑1 (MQ=255)

                                        tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                  <  2:3012312/128‑1 (MQ=255)

                                        tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                  <  1:1521558/128‑1 (MQ=255)

                                         aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                 >  2:270421/1‑128 (MQ=255)

                                          aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                <  1:3155910/128‑1 (MQ=255)

                                          aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                <  2:1958485/128‑1 (MQ=255)

                                              tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                            >  1:3007955/1‑128 (MQ=255)

                                              tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                            <  2:2835258/128‑1 (MQ=255)

                                               attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                           >  2:1425890/1‑128 (MQ=255)

                                                   tATCAATTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                       <  1:2319216/128‑1 (MQ=255)

                                                    aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                      <  2:2761578/128‑1 (MQ=255)

                                                    aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                      <  2:2135077/128‑1 (MQ=255)

                                                    aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                      <  1:2466421/128‑1 (MQ=255)

                                                    aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                      <  2:1539651/128‑1 (MQ=255)

                                                     tCAATTAAAAAAATAATACTTTTATTAGTTTATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                     <  1:1272967/128‑1 (MQ=255)

                                                      cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                    >  2:2015055/1‑128 (MQ=255)

                                                       aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                    >  1:2728508/1‑79 (MQ=255)

                                                        tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                  <  2:2086450/128‑1 (MQ=255)

                                                        tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                  >  1:2668290/1‑128 (MQ=255)

                                                        tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                  >  2:1804014/1‑128 (MQ=255)

                                                        tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                  >  1:2838234/1‑128 (MQ=255)

                                                        tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTATCAAATTGTTAAATAATTTTCAATTCATTATCTATTa                                                                  >  2:2636522/1‑128 (MQ=255)

                                                         ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                 <  2:405112/128‑1 (MQ=255)

                                                          tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAt                                                                >  1:3275191/1‑128 (MQ=255)

                                                              aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAt                                                            >  1:389979/1‑128 (MQ=255)

                                                                aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                          <  2:3078917/128‑1 (MQ=255)

                                                                aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTATAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                          <  1:493203/128‑1 (MQ=255)

                                                                 ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                         >  2:2960806/1‑128 (MQ=255)

                                                                 ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                         >  1:2960703/1‑128 (MQ=255)

                                                                    ataATTTTATTATTTTATATATTAGAAAACTACTATCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑g                                                    <  2:2041810/128‑1 (MQ=255)

                                                                     taCTTTTATTAGTTGATATATTAGAATACAACTTGCACTTTTTTAAAAAATTTTTAATTTTAATTACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑Gc                                                   <  1:1441858/128‑1 (MQ=255)

                                                                     taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑ttc                                                    >  2:1345970/1‑126 (MQ=255)

                                                                       cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTc                                                 >  2:171140/1‑128 (MQ=255)

                                                                        ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑TCTCg                                                >  1:2602898/1‑128 (MQ=255)

                                                                        ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTct                                                >  2:839368/1‑127 (MQ=255)

                                                                        ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCg                                                >  2:3116593/1‑128 (MQ=255)

                                                                         tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑t                                              >  1:2511315/1‑128 (MQ=255)

                                                                         tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑t                                              >  2:2257220/1‑128 (MQ=255)

                                                                         tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAATTTTA‑T‑GCTctt                                               >  1:753270/1‑126 (MQ=255)

                                                                            attatttttaatatTATAAAACTACAATCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTAAAATTTTTAAATTATTTTAAAATCATTATATATTAAACAATTTTA‑T‑GCTCG‑tttt                                           <  2:687505/118‑1 (MQ=255)

                                                                            attaGTTTATATATTAGAAAACTACTATCAATTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑tttt                                           <  2:3175815/128‑1 (MQ=255)

                                                                            attaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑tttt                                           >  1:3239672/1‑128 (MQ=255)

                                                                              tattttatatatTATAAAAATACTATAATATTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATAAAAAATTAATCAATTTTA‑T‑GCTCG‑tttttt                                         <  2:503344/122‑1 (MQ=255)

                                                                               attttatatatTATAAAAATAATATAAATTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTTTTAAATTATTTTAAAATCATTATATTTTAATCAATTTTA‑T‑GCTCG‑ttttttt                                        <  1:100121/123‑1 (MQ=255)

                                                                                    atatatTATATAAATAATATCAATATTTTAAAAAAATTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTATTTTAAAATCATTATCTATTAATCAAATTTA‑T‑GCTCG‑tttttttttttt                                   <  2:23522/128‑1 (MQ=255)

                                                                                      atatTAGAAAACTACTATCAATTTTTTAAAAAATTTTTAAATATAAAAACTGAATTATTTTTCAAAATTTTAAATTATTTTAAAATCATAAACAAATAATCAATTTTA‑T‑GCGCG‑tttttttttttttt                                 <  2:3202474/128‑1 (MQ=255)

                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCT‑tttttttttttttt                                 >  2:1592116/1‑128 (MQ=255)

                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑tttttttttttttt                                 >  2:252485/1‑128 (MQ=255)

                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTTTCTATAAATAAATTTTA‑T‑GCTCG‑tttttttttttttt                                 >  1:2129311/1‑128 (MQ=255)

                                                                                            gAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTTATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑tttttttttttttttacaaa                           <  1:2558289/128‑6 (MQ=255)

                                                                                               aaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑TTTTTTTTTTTTTTTTTTAaaaa                        >  1:3101654/1‑128 (MQ=255)

                                                                                                 ctactaTTAATTTTTTAAAAAATTTTTAATTTTAATAAATTTATTATTTTTTAAATTTTTAAATTATTTTCAAATAAATATATATTAATAAAATTTA‑T‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAat                      <  2:110628/128‑1 (MQ=255)

                                                                                                 ataatataaattttttAAAAAATTTTTTATTTTAATAAATTAATTATTTTTAAAAATTTTAAATTATTTTAAAATAAATATATATTAATAAATTTTA‑T‑TCGCT‑TTTTTTTTTTTTTTTTTACAAAAat                      <  2:2308590/118‑1 (MQ=255)

                                                                                                  ttatataatttttttAAAAATTTTTTAATTTTAATAAATTAATTTTTTTTTAATTTATTAAATTATTTTAAAATAATTATTTATTAATAAATTTTT‑T‑TTTCGTTTTTTTTTTTTTTTTTTACAAAAat                      <  1:450606/119‑1 (MQ=255)

                                                                                                   actatctatttattAAAAAAATTATAATT‑TTATAACTGTATTTTTTTTTCATTTGTTTAAATATTTTTAAAATATTATTAATTTATAAATTTTA‑TGGGTCG‑‑TTTTTTTTTTTTTTTTACAAAAAtata                   <  2:1860016/120‑1 (MQ=255)

                                                                                                   actaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAattt                    >  1:912243/1‑126 (MQ=255)

                                                                                                      ataatttttttAAAAAATTTTTTATTTTTATAAATTTATTTTTTTTTAAATTTTTTAATTATTTTAAAATTATTATTTTTTTAATAATTTTATT‑GTTTT‑TTTTTTTTTTTTTTTTTACAAAAATATaa                  <  1:1336464/123‑1 (MQ=255)

                                                                                                      aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑‑TTTTTTTTTTTTTTTTACAAAAATATAatt                >  1:1105894/1‑128 (MQ=255)

                                                                                                       gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑TTTTTTTTTTTTTTTTTAAAAAAATATAatt                >  2:348978/1‑128 (MQ=255)

                                                                                                       gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑‑TTTTTTTTTTTTTTTTAAAAAAATAAAaatt               >  2:762479/1‑128 (MQ=255)

                                                                                                        taattttttAAAAAATTTTTAATTTTAATAAATTTATTATTTTTTAAATTTTTAAATTATTTTAAAATAATTATTTATTAATAAATTTTA‑T‑TGTTG‑‑TTTTTTTTTTTTTTTTACAAAAATATAattta              <  1:1192764/125‑1 (MQ=255)

                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCT‑TTTTTTTTTTTTTTTTTAAAAAAAAATAatttatt            >  2:1209144/1‑128 (MQ=255)

                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑‑TTTTTTTTTTTTTTTTACAAAAATATAatttattt           >  1:708169/1‑128 (MQ=255)

                                                                                                               ttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑‑TTTTTTTTTTTTTTTTACAAAAATATAATttattttatt       >  1:2817562/1‑128 (MQ=255)

                                                                                                               ttAAAAAATTTTTAAATTTAATAACTGAATAATTTTTAAAATTGTTAAAATATTTTCAAATAATTATCTATTAATCAATTTTA‑T‑GCTCG‑‑TTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt       <  1:3325313/128‑1 (MQ=255)

                                                                                                                    aaaTTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑TTTTTTTTTTTTTTTTTAAAAAAATATTATTTATTTAATTAAAc   >  1:1714293/1‑128 (MQ=255)

                                                                                                                     aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑TTTTTTTTTTTTTTTTAAAAAAAATAAAATTAATTTTATaaaaat  >  2:1372838/1‑126 (MQ=255)

||

TAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑T‑GCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACG  >  CP016816/420035‑420279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`