Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,801 A→G intergenic (‑110/+61) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,8010AG98.0% 5792.9 / NA 1621intergenic (‑110/+61)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (8/0);  major base G (743/832);  minor base T (28/5);  total (784/837)
Fisher's exact test for biased strand distribution p-value = 1.20e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1763 total aligned reads displayed.

AAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484674‑484925
                                                                                                                                |                                                                                                                            
  aaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCa                                                                                                                            <  2:1038005/128‑1 (MQ=255)
     aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                                         <  1:2785963/128‑1 (MQ=255)
     aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                                         <  1:1124156/128‑1 (MQ=255)
     aGTCAAAACTCATAGATTTAATACTTTAATATTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                                         <  1:1564926/128‑1 (MQ=255)
          aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                                    >  1:2478245/1‑128 (MQ=255)
            aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                                  >  1:3081071/1‑128 (MQ=255)
            aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTATAATATAAAAAGCAATTAAacaca                                                                                                                  >  2:2401816/1‑128 (MQ=255)
             cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                                 >  2:177610/1‑128 (MQ=255)
              tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                                >  1:427514/1‑128 (MQ=255)
               cATAGAATTAATCCTTTTATAGTTGAACAAAAAATAAGATATAGCAAATCAGAAGCTCTTAACAACTAAACTACACTCGATTAATTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                                <  2:2072374/128‑1 (MQ=255)
                 tAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                             <  2:1677144/128‑1 (MQ=255)
                 tAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                             <  2:1829111/128‑1 (MQ=255)
                    aTTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACt                                                                                                          <  1:2591146/128‑1 (MQ=255)
                       tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCTATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                                       <  2:2529226/128‑1 (MQ=255)
                       tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATTTAAAAAGCAATTAAAAACAAAAAAAcaaac                                                                                                       >  1:84346/1‑124 (MQ=255)
                       tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                                       <  2:414587/128‑1 (MQ=255)
                        aaTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcc                                                                                                      >  1:1928421/1‑128 (MQ=255)
                         ttCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATATCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                                     <  2:2467921/127‑2 (MQ=255)
                         aTCCTTTTATAGTTGACCAAAAAATAAGATATAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                                     <  1:1737192/128‑2 (MQ=255)
                          tacattattaGTTGACCAAAAAATAAGATAGATCATATCATAAGATCATAACAACTAAACTACAATCGATTATTTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCa                                                                                                    <  2:1544938/120‑1 (MQ=255)
                          tCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCa                                                                                                    <  2:1611515/128‑1 (MQ=255)
                           ccTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAt                                                                                                   <  2:2747425/128‑1 (MQ=255)
                             ttttATAGTTGACCAAAAAATAAGATAGAGCTTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                                 <  1:1837179/128‑1 (MQ=255)
                             ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                                 <  2:57702/128‑1 (MQ=255)
                              tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                                >  2:2801580/1‑128 (MQ=255)
                                 ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                             <  2:1942172/128‑1 (MQ=255)
                                 ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                             <  2:63601/128‑1 (MQ=255)
                                  taGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATGGTg                                                                                            >  2:139216/1‑128 (MQ=255)
                                  taGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTATAATATAAAAATCAATTAAACACACAAAAACTCAACCATTATtgtg                                                                                            >  2:2208551/1‑128 (MQ=255)
                                   aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                           <  1:3149567/128‑1 (MQ=255)
                                   aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                           >  1:1515781/1‑128 (MQ=255)
                                   aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                           <  2:3213410/128‑1 (MQ=255)
                                        aCCAAAAAATAAGATAGAGAATAGCAGAAGCTAATAACAACTAAACTACACTCGATTAATTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                                      <  2:3063064/128‑1 (MQ=255)
                                           aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACTCACAAAAACACACACATTATGCTGAGTTAaaaa                                                                                   >  1:1801283/1‑128 (MQ=255)
                                           aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTGTaaaa                                                                                   >  1:3320806/1‑128 (MQ=255)
                                           aaaaaaTAAGATAGAGCAAAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                                   <  2:2279719/128‑1 (MQ=255)
                                            aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  1:1063911/128‑1 (MQ=255)
                                              aaaTAAGATAGAGCATAGCAGAAGCTATTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAACAAATAAACACACAAAAACAAACACATAATGGTGAGTTTAAAAatt                                                                                >  1:3212522/1‑128 (MQ=255)
                                                 tAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAattatt                                                                             <  2:1297015/128‑1 (MQ=255)
                                                   aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                            >  2:2332892/1‑128 (MQ=255)
                                                   aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                           >  1:1013188/1‑128 (MQ=255)
                                                        gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTTTGGTGAGTATAAAAATTATTAAAAAAt                                                                      >  1:709574/1‑128 (MQ=255)
                                                        gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTTAAAAATATTAAAAAAt                                                                      >  1:1679489/1‑128 (MQ=255)
                                                        gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                                      >  2:1239575/1‑128 (MQ=255)
                                                         agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAATAATTATTCAAAAAtt                                                                     >  2:14971/1‑128 (MQ=255)
                                                         agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                                     >  1:1332877/1‑128 (MQ=255)
                                                          gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                                    >  1:1196580/1‑128 (MQ=255)
                                                          gCATAGCAGAAGCTCTTAACAACTAAACGACACTCGATTATTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTg                                                                   >  2:2564858/1‑128 (MQ=255)
                                                           cATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTATAAAAATTATTCAAAAAATTg                                                                   >  2:1430721/1‑128 (MQ=255)
                                                            aTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                                  <  2:695190/128‑1 (MQ=255)
                                                             tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                                 <  1:1876878/128‑1 (MQ=255)
                                                             tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                                 >  1:2424568/1‑128 (MQ=255)
                                                                cagaatttaatAAAAACTAAAATTAAATCGTTTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattat                                                              <  1:1440134/118‑1 (MQ=255)
                                                                  gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATTGTGAGTTTAAAAATAATACAAATATTTGATAAttt                                                            >  1:1387086/1‑128 (MQ=255)
                                                                   aaGCTCTTAACAACTAAACTACACTCGATTATTTAATTATAATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGTGTTTAAAAATTATTCAAAAATTTCATTAtttt                                                           >  1:2004337/1‑128 (MQ=255)
                                                                     gCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                                         >  1:480457/1‑128 (MQ=255)
                                                                       tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAAt                                                       <  2:2646284/128‑1 (MQ=255)
                                                                       tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAAt                                                       >  1:388465/1‑128 (MQ=255)
                                                                         ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGATTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                                     >  1:2834144/1‑128 (MQ=255)
                                                                         ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATATGATAATTTTAAATCa                                                     >  2:462545/1‑128 (MQ=255)
                                                                           aacaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                                   <  2:827099/128‑1 (MQ=255)
                                                                            acaacTTATCTACAGTCGATTATTTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                                  <  1:2932827/128‑1 (MQ=255)
                                                                            acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                                           >  1:2527088/1‑71 (MQ=255)
                                                                            acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                                  <  1:2374119/128‑1 (MQ=255)
                                                                            acaacTAAACTAAAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                                  <  1:1618417/128‑1 (MQ=255)
                                                                              aactacactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                                <  1:542674/128‑1 (MQ=255)
                                                                              aaataaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                                <  1:2143520/125‑1 (MQ=255)
                                                                               actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                               <  2:333124/128‑1 (MQ=255)
                                                                                ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                              <  2:1112873/128‑1 (MQ=255)
                                                                                      taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGATTTTAAAAATTAATCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                                        >  1:2085282/1‑128 (MQ=255)
                                                                                      taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                                        <  2:880665/128‑1 (MQ=255)
                                                                                      taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                                        <  1:2726610/128‑1 (MQ=255)
                                                                                       acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                               <  1:3280215/120‑1 (MQ=255)
                                                                                       aaaatagATTATTTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                       <  1:2979118/122‑1 (MQ=255)
                                                                                           tCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTGAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                                   <  2:2006280/128‑1 (MQ=255)
                                                                                               ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTa                               >  2:503496/1‑128 (MQ=255)
                                                                                                 attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                             <  2:1181562/128‑1 (MQ=255)
                                                                                                 attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                             <  1:761754/128‑1 (MQ=255)
                                                                                                 attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                             >  2:1873054/1‑128 (MQ=255)
                                                                                                   ttAATTATATTTTTTTTTAATATAAAAAGCAAATAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCTAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                           <  2:3013342/128‑1 (MQ=255)
                                                                                                    tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGATTTTAAAAATTATAAAAAAATTTGATTATTTTAAATAAATTGAAGCTCCACCTTCAACTAATTTa                          >  2:3276184/1‑128 (MQ=255)
                                                                                                     aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg                         >  1:98749/1‑128 (MQ=255)
                                                                                                     aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg                         <  2:2698079/128‑1 (MQ=255)
                                                                                                     aaTTATATTTTATTTTATTATAAAAAGGATTTAAACACACAATATCTGAACCATTATGTTGAGATTAAAAATTATTCTTAAATTAGTTTATTTTAAATCAAATGAAGCTCCAGCTTCAACTAATTTAg                         <  2:1734113/128‑1 (MQ=255)
                                                                                                         atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt                     >  2:780848/1‑128 (MQ=255)
                                                                                                         atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt                     >  2:94029/1‑128 (MQ=255)
                                                                                                         atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt                     >  1:3026421/1‑128 (MQ=255)
                                                                                                         atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACACACCCATAATGTTGAGTTTAAAAATTATTCAAAATATTGATTATTTTAAATCAATTTAAGCTCAAGCTTCAACTAATTTAGCttt                     >  1:936069/1‑128 (MQ=255)
                                                                                                          tatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGATGCTCCAGCTTCAACTAATTTAGCTTTc                    <  2:2889493/128‑1 (MQ=255)
                                                                                                          tatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAATCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTc                    <  1:636661/128‑1 (MQ=255)
                                                                                                            tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAATTTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCACCTAATTTAGCTTTCAt                  >  1:2882950/1‑128 (MQ=255)
                                                                                                                  ttttaatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAATAATTTAGCTTTCATttctt             >  1:1275967/10‑128 (MQ=255)
                                                                                                                  ttttaatataaaaagCAATTAAAAACACAAAAACTCACTCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttctt             <  1:2245229/119‑1 (MQ=255)
                                                                                                                     taatataaaaatCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTTTTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAg          <  2:2849356/122‑1 (MQ=255)
                                                                                                                     taatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt                 <  1:2664638/115‑1 (MQ=255)
                                                                                                                      aatttaaaaagCAATAAAACACACAAAAACTCACCCATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGc         <  2:2117777/123‑1 (MQ=255)
                                                                                                                      aatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCACCTAATTTAGCTTTCATatcttccga         >  2:2967918/6‑125 (MQ=255)
                                                                                                                       atataaaaagCAATTAAACACTCAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGct        <  2:1347311/124‑1 (MQ=255)
                                                                                                                           aaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                                         >  2:3132564/1‑75 (MQ=255)
                                                                                                                           aaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGcttctt    >  1:231418/1‑128 (MQ=255)
                                                                                                                                |                                                                                                                            
AAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484674‑484925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: