Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,791 T→A intergenic (‑100/+71) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7910TA98.2% 4735.5 / 18.5 1605intergenic (‑100/+71)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base T (16/12);  new base A (778/798);  total (795/810)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1666 total aligned reads displayed.

TATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTT  >  CP016816/484664‑484914
                                                                                                                               |                                                                                                                           
           aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                  <  2:1976327/128‑3 (MQ=255)
           aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                  >  2:2424819/1‑126 (MQ=255)
           aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTATTTTTTAATATAAAAACa                                                                                                                 >  2:1406172/1‑128 (MQ=255)
              tAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                               >  1:1440400/1‑128 (MQ=255)
              tAGTAAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAAAAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                               <  1:460453/128‑1 (MQ=255)
               aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                              <  1:1124156/128‑1 (MQ=255)
                  cAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                           <  1:1284166/128‑1 (MQ=255)
                   aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGAATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAAATATATTTTTTTTTAATATAAAAATCAATTAAac                                                                                                          >  1:3306475/1‑128 (MQ=255)
                    aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                         >  1:1735114/1‑128 (MQ=255)
                    aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                         >  1:2478245/1‑128 (MQ=255)
                        tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                     >  1:2787066/1‑128 (MQ=255)
                        tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                     <  2:1212816/128‑1 (MQ=255)
                             gATTTAATCCTTTTATAGTTGACCAAAAAATAAGATATATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                                <  2:2990890/128‑1 (MQ=255)
                              ttttAATCCTTTTATAGTTGAACAAAAAATAAGATAGAGCCTAGCAGAATGTCTTAAGTACTAAACTACACTCGATTATTTACTTTTATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAATCt                                                                                               <  1:2159143/127‑1 (MQ=255)
                              aTTTAATCCATTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACt                                                                                               <  2:1335807/128‑1 (MQ=255)
                               attAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                              <  1:325048/127‑1 (MQ=255)
                                 tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAAAAATTAACAAAACAAAAACTCAc                                                                                            >  1:3182779/1‑128 (MQ=255)
                                 tAATCCTTTTATAGTAGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                            <  2:3077441/128‑1 (MQ=255)
                                  aaTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcc                                                                                           >  1:3000588/1‑128 (MQ=255)
                                  aaTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcc                                                                                           >  2:2857356/1‑128 (MQ=255)
                                     ccTTTTCTAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAAAAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAt                                                                                        <  1:3128400/128‑1 (MQ=255)
                                       ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataat                                                                                      <  2:256940/128‑6 (MQ=255)
                                       ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                      <  2:2748563/128‑1 (MQ=255)
                                        tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAatat                                                                                     >  2:2143520/1‑128 (MQ=255)
                                        tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                     >  2:270528/1‑128 (MQ=255)
                                          tttaGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                   <  2:1582871/126‑1 (MQ=255)
                                          tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAAAACACAAAAACTCACCCATTAttg                                                                                   >  2:2299386/1‑126 (MQ=255)
                                           ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                  <  1:613991/128‑1 (MQ=255)
                                            taGTTGACCAAAAAATAAGAAAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTg                                                                                 <  1:732271/128‑1 (MQ=255)
                                             aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACTAAAACTCACCCATTATGTTGa                                                                                >  2:3163975/1‑128 (MQ=255)
                                             aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                <  1:412703/128‑1 (MQ=255)
                                             aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                <  1:1890645/128‑1 (MQ=255)
                                                tGACCAAAAAAAAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACCATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATAATGGAGAGtt                                                                             >  1:94706/1‑128 (MQ=255)
                                                  aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                           <  1:2429801/128‑1 (MQ=255)
                                                  aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                           <  2:1275163/128‑1 (MQ=255)
                                                  aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATGATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                           >  2:3293342/1‑128 (MQ=255)
                                                   ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                          >  1:2889493/1‑128 (MQ=255)
                                                   ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                          <  2:369987/128‑1 (MQ=255)
                                                     aaaaaaTAATATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAACCACACAAAAACTCACCCATGATGGTGAGTTTaaaa                                                                        >  1:1562913/1‑128 (MQ=255)
                                                      aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                       <  1:1063911/128‑1 (MQ=255)
                                                      aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                       <  1:2953645/128‑1 (MQ=255)
                                                      aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                       >  1:2375002/1‑128 (MQ=255)
                                                      aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                       <  1:19375/128‑1 (MQ=255)
                                                        aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                     <  2:2532150/128‑1 (MQ=255)
                                                         aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAACCTACACTCGATTATTTAATAATATTTTTTTTTAATATAAATAGCAATTATCAACACAAAAACTCACCCAATATCGTGAGTTTAAAAaata                                                                    >  1:689843/1‑128 (MQ=255)
                                                             aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACCCTCGCTTAGTTAAGTATATTTTTTTTTAATATAAAAAGCAATTAAAACCACAAAAACTCACCCATTATGGGGAGGTTTCAACTTATTCa                                                                >  1:363251/1‑128 (MQ=255)
                                                             aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                <  2:2375002/128‑1 (MQ=255)
                                                             aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                >  1:1013188/1‑128 (MQ=255)
                                                              gATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaa                                                               <  1:1518663/128‑1 (MQ=255)
                                                              gATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaa                                                              <  1:1841097/128‑1 (MQ=255)
                                                                  gagCATATCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                           <  2:1898313/128‑1 (MQ=255)
                                                                  gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTATAAATTATTCAAAAAt                                                           >  2:881999/1‑128 (MQ=255)
                                                                  gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                           >  2:290546/1‑128 (MQ=255)
                                                                   agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                          >  2:93637/1‑128 (MQ=255)
                                                                   agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                          <  1:2312858/128‑1 (MQ=255)
                                                                   agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                          >  1:3063863/1‑128 (MQ=255)
                                                                    gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                         >  1:1196580/1‑128 (MQ=255)
                                                                          cAGAAGCTATTAACAACAAAACTACAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattat                                                   <  2:1137734/128‑1 (MQ=255)
                                                                           aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                   <  1:2254439/95‑1 (MQ=255)
                                                                           aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                  >  1:2733792/1‑128 (MQ=255)
                                                                            gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                 >  1:2664058/1‑128 (MQ=255)
                                                                                 tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAAt                                            <  2:2646284/128‑1 (MQ=255)
                                                                                 tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAAt                                            <  2:223655/128‑1 (MQ=255)
                                                                                  ctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                           >  2:790334/1‑128 (MQ=255)
                                                                                   ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                          <  2:1077367/128‑1 (MQ=255)
                                                                                   ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                          >  2:1530890/1‑128 (MQ=255)
                                                                                    tAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGATTAAAAATTATTCAAAAATATTATTATTTTAAATCaa                                         >  2:422789/1‑128 (MQ=255)
                                                                                      acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                       <  2:1369714/128‑1 (MQ=255)
                                                                                      acaacTAAACTACACTCGATTATTTAATTATATTTTATTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGGGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                       <  2:513135/128‑1 (MQ=255)
                                                                                      acaaaTAAACTACACTCGATTAATTAATTATATTTTTTTTTAATAAAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                       <  1:2603693/128‑1 (MQ=255)
                                                                                      aaaaataaaataAACTAGATTATTTAATTTTATTTTTTTTTAATATTAAAATCAATTAAACACAAAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                       <  1:512682/123‑1 (MQ=255)
                                                                                        aactaaactaCACTCGATTATTTAATTATTATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                     <  2:1644303/128‑1 (MQ=255)
                                                                                        aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                     <  2:100256/128‑1 (MQ=255)
                                                                                         actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                       <  1:792731/93‑1 (MQ=255)
                                                                                         actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                    <  2:170338/128‑1 (MQ=255)
                                                                                         actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                    <  2:2478245/128‑1 (MQ=255)
                                                                                         actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                    >  1:2012447/1‑128 (MQ=255)
                                                                                            aaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAACACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCt                                 <  1:1343506/128‑1 (MQ=255)
                                                                                            aaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTAAATCt                                 >  1:2849356/1‑128 (MQ=255)
                                                                                              actaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAACCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAtctca                               >  2:303339/1‑127 (MQ=255)
                                                                                               ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCTAAAATTTGATTATTTTAAATCAATAGAAGCTACa                              >  2:1467925/1‑128 (MQ=255)
                                                                                                taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGATTTTAAAAATTAATCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                             >  1:2085282/1‑128 (MQ=255)
                                                                                                taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                             <  1:1803381/128‑1 (MQ=255)
                                                                                                 acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                          <  1:1618162/114‑1 (MQ=255)
                                                                                                        attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                     >  2:2023132/1‑128 (MQ=255)
                                                                                                         ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTa                    >  1:1669792/1‑128 (MQ=255)
                                                                                                         taattTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTAACCAATTATGGTGAGATTAAAAATTATACAAAAAATTCATTAATATAAATAAATTGAAGCTCCAGCATCAAcaa                    >  2:1846834/3‑126 (MQ=255)
                                                                                                          tattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATTATTCAAAAAATAGATTATTTTAAATCAATTGAATCTCCACCTTCAACaaa                   >  2:474524/1‑128 (MQ=255)
                                                                                                           attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCCCCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                  >  2:1814388/1‑128 (MQ=255)
                                                                                                            tttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAtt                 <  2:2802174/128‑1 (MQ=255)
                                                                                                             ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                >  2:668442/1‑128 (MQ=255)
                                                                                                             ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                <  1:2530260/128‑1 (MQ=255)
                                                                                                               aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg              <  1:872116/128‑1 (MQ=255)
                                                                                                               aaTTATAGTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTGGAGCTTCAATTGATTTaa              <  2:612758/128‑2 (MQ=255)
                                                                                                                 ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTaa                   >  2:1759281/1‑121 (MQ=255)
                                                                                                                 ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCt            <  2:707492/128‑1 (MQ=255)
                                                                                                                   atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt          <  2:209653/128‑1 (MQ=255)
                                                                                                                   atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACACACCCATTATGGTGAGTTTAAAAATTATTAAAAAATTAGATAATTTTAAATCAATTGAAGCTCCAGCATCAACTAATTTATCttt          >  1:1399696/1‑128 (MQ=255)
                                                                                                                     atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCa        >  2:3238124/1‑128 (MQ=255)
                                                                                                                      tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt       >  2:835538/1‑128 (MQ=255)
                                                                                                                               |                                                                                                                           
TATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTT  >  CP016816/484664‑484914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: