Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,157 2 bp→TT intergenic (‑440/+402) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1570AT99.2% 4026.2 / NA 1309intergenic (‑440/+403)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (6/4);  new base T (673/624);  total (679/630)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1580AT98.6% 3871.0 / NA 1309intergenic (‑441/+402)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (3/16);  new base T (674/614);  total (679/630)
Fisher's exact test for biased strand distribution p-value = 1.84e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1381 total aligned reads displayed.

TTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420034‑420285
                                                                                                                            ||                                                                                                                                  
  aaattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattt                                                                                                                               >  2:268517/1‑127 (MQ=255)
    attcattcATACTTTTAGTCTCATTAATCTAAGTTTCTAAAATTATTTTATGATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                             <  1:2220562/128‑4 (MQ=255)
    attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                             >  2:3223416/1‑125 (MQ=255)
    attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                             <  2:433310/128‑4 (MQ=255)
    attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                             >  1:3801220/1‑125 (MQ=255)
      tcattcctAATTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaat                                                                                                                           <  2:1476337/128‑6 (MQ=255)
      tcattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaat                                                                                                                           <  1:1005802/128‑6 (MQ=255)
       cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTATAAAACTACTAGCACTTATATAAAAAATTTAAAATTTTAATa                                                                                                                          >  2:2621901/1‑128 (MQ=255)
       cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                          <  2:1884498/128‑1 (MQ=255)
        attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                         >  2:3004345/1‑128 (MQ=255)
        attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                         >  1:2019182/1‑128 (MQ=255)
         ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                        >  2:856220/1‑128 (MQ=255)
          tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                       >  2:583115/1‑128 (MQ=255)
          tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                       <  2:2862620/128‑1 (MQ=255)
             tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                    <  1:1381952/128‑1 (MQ=255)
              aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                   <  1:287054/128‑1 (MQ=255)
              aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                   >  2:2016785/1‑128 (MQ=255)
              aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                   <  1:3495915/128‑1 (MQ=255)
                 tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                <  1:2338074/128‑1 (MQ=255)
                 tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                <  1:1561431/128‑1 (MQ=255)
                 tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                <  1:1322407/128‑1 (MQ=255)
                  ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                               >  1:3757899/1‑128 (MQ=255)
                  ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                               <  2:3466889/128‑1 (MQ=255)
                   tAGTCTCATAAATCTAATTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                              <  1:406502/128‑1 (MQ=255)
                      tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                           >  2:3685904/1‑128 (MQ=255)
                       ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                          <  1:3333052/128‑1 (MQ=255)
                          aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                       >  2:226065/1‑128 (MQ=255)
                             aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                    <  2:180074/128‑1 (MQ=255)
                                 tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                <  1:1294997/128‑1 (MQ=255)
                                    gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAACTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                             >  2:1177304/1‑128 (MQ=255)
                                      ttCTAAAATTATTTTATCAATTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           <  2:2765482/128‑1 (MQ=255)
                                       tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTCAATTATTTTTCAAATTGTTAAATTAttt                                                                                          >  1:886858/1‑128 (MQ=255)
                                        cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                         >  2:3827403/1‑128 (MQ=255)
                                        cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTATTCAAATTTATAAAATAtttt                                                                                         >  1:3537940/1‑128 (MQ=255)
                                          aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                       <  1:696923/128‑1 (MQ=255)
                                           aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTTAAATTGTTAAATTATTTTc                                                                                        >  2:1969219/1‑126 (MQ=255)
                                            aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTATTTTaaaa                                                                                     >  1:2519080/1‑128 (MQ=255)
                                            aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                     <  2:2465802/128‑1 (MQ=255)
                                             attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                    <  1:1799137/128‑1 (MQ=255)
                                                attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                                 >  2:220225/1‑128 (MQ=255)
                                                   ttatactttAAATAAATAATACTTTTATTAGATGATAAATTAGAACACTTCTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                              <  2:779845/122‑1 (MQ=255)
                                                   ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                              <  2:393224/128‑1 (MQ=255)
                                                    tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                             <  1:263184/128‑1 (MQ=255)
                                                     aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                            <  1:973100/128‑1 (MQ=255)
                                                     aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                            >  2:2958708/1‑128 (MQ=255)
                                                     aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                            <  1:2939357/128‑1 (MQ=255)
                                                      tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                           >  2:3299648/1‑128 (MQ=255)
                                                      tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                           <  1:2574161/128‑1 (MQ=255)
                                                       cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                          >  2:3620152/1‑128 (MQ=255)
                                                        aTTTAAAAAAATAATACTTTTATTAGTTGATATATTTGAAAACTACTAGCACTTTTTTAAAAAATTTTTATTTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                         >  1:1877522/1‑128 (MQ=255)
                                                        aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                         <  2:1578689/128‑1 (MQ=255)
                                                        aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                         <  2:2342784/128‑1 (MQ=255)
                                                         tatAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTATTTTAAAAAATTTTTAATTTTAATAACTGAATAATTTTTCAAATTGTTAAAATATTTTCAAATCATTATCAATTa                                                                        >  2:70602/3‑128 (MQ=255)
                                                           tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAt                                                                      <  1:1626505/128‑1 (MQ=255)
                                                            aaaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTATTTTAAAAATATTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAAATATTAATc                                                                     >  1:328689/1‑128 (MQ=255)
                                                                aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaaat                                                                 >  2:3387042/1‑126 (MQ=255)
                                                                aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                                 <  1:3003522/128‑1 (MQ=255)
                                                                  ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                               >  2:958051/1‑128 (MQ=255)
                                                                     ataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAATTTCAAATCATTATCTATTAATCAATTTTATg                                                            >  1:2716533/1‑128 (MQ=255)
                                                                      taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                           >  1:3151855/1‑128 (MQ=255)
                                                                           ttattaTTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCAATTAATCAATTTTATGCTCG‑‑tt                                                    <  1:3677282/128‑1 (MQ=255)
                                                                           ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tt                                                    >  1:545498/1‑128 (MQ=255)
                                                                            tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTCt‑‑ttt                                                   >  1:3858720/1‑128 (MQ=255)
                                                                            tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttt                                                   >  2:1103475/1‑128 (MQ=255)
                                                                            tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttt                                                   >  1:3098036/1‑128 (MQ=255)
                                                                                aGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttt                                              >  2:3550070/1‑128 (MQ=255)
                                                                                  tttatatatTACAAAACAAATAACAATTTTTTAAAAAATTTTTTATTTTTATAAATTAATTATTTTTCAAATTTTTAAATTATTTTAAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttttt                                            <  1:757184/125‑1 (MQ=255)
                                                                                  ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttttt                                            >  2:3767370/1‑128 (MQ=255)
                                                                                   tGATATATTAGAAAACTACAAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATATTTTTTCAAATTGTTAAATTATTTTCAAATCAATATCTATTAATCAATTTTATGCCCG‑‑tttttttttt                                           <  1:2113990/128‑1 (MQ=255)
                                                                                    gATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tattttttttt                                          >  1:1692932/1‑128 (MQ=255)
                                                                                        tattatAAAAATAATATAAATTTTTTAAAAAAATTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATCAAATTTATGCTCG‑‑ttttttttttttttt                                      <  1:615618/128‑1 (MQ=255)
                                                                                          ttttaaaaCTTCTAGCAATTTTTTAAAAAATTTTTAATTTTAATAAAATAATTATTTTTAAAATTTTTAAATTATTTTAAAATCATTATATATAAATCAAATTTATGCTCG‑‑ttttttttttttttttt                                    <  1:3918356/124‑1 (MQ=255)
                                                                                          tttgAAAAATACTATGACTTTTTTAAAAAATTTTTATTTTTAATAATTGTATTATTTTTCAAATTGTTTAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttttttttttttt                                    <  1:3432696/125‑1 (MQ=255)
                                                                                          ttAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttttttttttttttta                                    >  2:1679568/1‑127 (MQ=255)
                                                                                            ataaaaATAATATCAATTTTTTAAAAAATTTTTAAATTTAAAAAATTAAATATTTTTCAAATTTTTAAATTTTTTTCAAATCAATATCTATTAATAAATTTTATTCTCG‑‑ttttttttttttttttaca                                  <  2:1314986/126‑4 (MQ=255)
                                                                                             gAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTaaa                                 >  2:3090981/1‑126 (MQ=255)
                                                                                               aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCT‑‑TTTTTTTTTTTTTTTTTAaaaa                               >  1:2438447/1‑128 (MQ=255)
                                                                                               aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACaaa                               >  2:2093606/1‑128 (MQ=255)
                                                                                                aaaaactaTCACTTTTTTTAAAAATTTTTAATTTTAAAAACTTAATTATTTTTCAAATTTTTAAATTATTTTCAAATCATAATCTATTAATCAATTTTTTTCTCGTTTTTTTTTTTTTTTTTTACaaa                               <  2:1376086/124‑1 (MQ=255)
                                                                                                  ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑‑‑TTTTTTTTTTTTTTTACAAAAatat                          >  1:2186092/1‑128 (MQ=255)
                                                                                                  atagaagtaattttttAAAAAATTTATATTTTTAAAAAATGAATTATTTTTAAAATTTTTAAAATATTTTAAAATAATTATATATTAATCAATTTTATTCTCGTTTTTTTTTTTTTTTTTTACaaaaa                             <  1:807074/118‑1 (MQ=255)
                                                                                                   tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TATTTTATATTTTTTTAAAAAAAata                           >  1:1745895/1‑128 (MQ=255)
                                                                                                    tatatgaattttttAAAAAATTTTTAATTTTAATAAATGAATTTATTTTAAAATTTTTAAATTATTTTCAAATTATTATATATTAATAAATTTTATGCTCGTTTTTTTTTTTTTTTTTTACAAAAata                           <  1:3062050/120‑1 (MQ=255)
                                                                                                     attgCAATTTTTTAAAAAATTTTTAATTTTATTAAATTAATTATTTTTCAAAATGTTAAATTATTTTAAAATCATTATATATTAATAAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAAtata                         <  1:3620152/125‑1 (MQ=255)
                                                                                                      tttcaatattttAATAAATTTTTAATTTTAATAAATTAATTTTTTTTAAAATTTTTAAATTATTTTAAAATAATTATCTATTAATAAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATaa                        <  1:3238510/120‑1 (MQ=255)
                                                                                                      tataaattttttAAATAATTTTTAATTTTAATAAATTAATTATTTTTCAATTTGTTAAATTATTTTTAAATCATTAACAATTTATCAATTTTTTGTTG‑‑GTTTTTTTTTTTTTTTTACAAAAATATAat                       <  2:1264437/122‑1 (MQ=255)
                                                                                                      tactaaatttttCAAATATTTTTAATTTTAAAAACTGAAATATATTTCAAATTGTTAAATTATTTTAAAATACTTATTTATTTATCAATTTTTTGCTCGTTTTTTTTTTTTTTTTTTACAAAAAtata                         <  1:1468836/121‑1 (MQ=255)
                                                                                                      taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTATTTTTTTTTTTAAAAAAAaata                         >  1:1072821/1‑128 (MQ=255)
                                                                                                        gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTATAG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAatt                      >  1:1220730/1‑128 (MQ=255)
                                                                                                        gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAatt                      >  1:472851/1‑128 (MQ=255)
                                                                                                        gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑‑‑‑TTTTTTTTTTTTTTACAAAAATATAatttat                   >  1:2776056/1‑128 (MQ=255)
                                                                                                         cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTTAAAAAAATATAat                       >  1:1144895/1‑128 (MQ=255)
                                                                                                            ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAAAATaaaattat                  >  2:1025285/1‑122 (MQ=255)
                                                                                                              ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATTTTATTTTTTTg                >  2:2570153/1‑128 (MQ=255)
                                                                                                               tttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTAAAAAAAAATAatttttttt                >  2:3155954/1‑127 (MQ=255)
                                                                                                               tttAAAAAATTTTTAAATTTAATAACTGAATTAATTTTCAAATATTTTAATTATTTTCAAATCATTATATATTAATCAAATTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa               <  2:488418/128‑1 (MQ=255)
                                                                                                                 tAAAAAATTTTTATTTTTAATAAATTATTTATTTTTAAATTTGTTAAATTATTTTAAAATAATTTACTATTAATAAATTTTATTCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt             <  1:1980955/128‑1 (MQ=255)
                                                                                                                   aaaatTTTTAATTTTAAATAATTTATTATTTTTTAAATTTTTTAATTTTTTTAAATTTATTTTTTTTTTATAAATTTTTTTTTTG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaaa          <  2:1750305/128‑1 (MQ=255)
                                                                                                                   aaaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAATTATAATTTATttaattaa           >  2:3869776/1‑128 (MQ=255)
                                                                                                                      aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTATTAAActt       >  1:2266307/1‑126 (MQ=255)
                                                                                                                            ||                                                                                                                                  
TTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420034‑420285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: