Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816203,9370CA31.1% 1583.1 / 109.3 956intergenic (+321/+137)JCVSYN2_00845/JCVSYN2_00850lipoprotein/inorganic diphosphatase
Reads supporting (aligned to +/- strand):  ref base C (308/331);  new base A (228/61);  total (543/413)
Fisher's exact test for biased strand distribution p-value = 3.85e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 970 total aligned reads displayed.

AAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCATTTTTTAATTTTGTTT  >  CP016816/203809‑204064
                                                                                                                                         |                                                                                                                               
  gcaaTTTTTAAAAAATCCTATGTTCTATGTTTTTTATTATACTATTCAAAATTAAAACGGGTTCAAATTG‑TTTTTTTTTGG‑C‑T‑TT‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCa                                                                                                                                <  2:2147668/125‑1 (MQ=255)
   caaTTTTTAAAAAAGCCTATTTTTTATGATTTTTATTATAATTATAAAAATTACAAAGTTTTCAAAGTG‑TTTTTTTTAGG‑CTT‑TT‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCa                                                                                                                                <  1:1940494/126‑1 (MQ=255)
   acATTTTTTAAAAAACTAATGTTTTATGCTTTTTATTATTATTATCAACATTTAAACCGTTTCAAATTGTTTTTTTTTTGG‑G‑TGTT‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATc                                                                                                                                 <  2:3617559/127‑1 (MQ=255)
     aTTTTTAAAAAAGCCAATGTTTTATGCTTTTTATTATAATTATCAAAATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAAcc                                                                                                                             <  2:3231505/128‑1 (MQ=255)
     aTTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAAAAAAAGTAAGA‑‑TTTTGATCaaac                                                                                                                             >  1:3761412/1‑126 (MQ=255)
       ttctAAAAAAGCATATCTTCTAGCCTTTTTAATATACTTAACAACATTAAAACCCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TT‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGT‑‑TTTGGTTGATCCaa                                                                                                                           <  1:3376447/125‑1 (MQ=255)
          tAAAAAATTCTTTTTTATTGGCTTTTTTTTATAATTATAAAAATTAAAACGGTTTAAAATTT‑TATTTTTTTGG‑T‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAAc                                                                                                                        <  2:1633102/128‑1 (MQ=255)
          tAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TGT‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGT‑‑TTTGGTTACACCaaac                                                                                                                         >  2:232356/1‑127 (MQ=255)
          tAAAAAAGACTATTTTTTATGCTTTTTATTATACTTATCAACATTAAAAAGCTTTCAAATTT‑TTTTTTTTTGG‑G‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAAc                                                                                                                        <  2:187676/128‑1 (MQ=255)
           aaaaaaGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGT‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTAT‑TAAAAAAAATATAAAAATTTTGTATCAAACaaaa                                                                                                                         >  1:2114119/1‑128 (MQ=255)
             aaaaGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑‑TTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACtata                                                                                                                    >  1:3091204/1‑128 (MQ=255)
              aaaGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAATAAAATTAATA‑‑TTTGGATAAAACaaaaaagat                                                                                                                    >  2:118637/1‑123 (MQ=255)
                 gCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑TAAAATAAAAGTTATA‑‑TTTGGTTCACCAAAAACTatataa                                                                                                                 >  1:2945444/1‑126 (MQ=255)
                    tATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAATTAATA‑‑TTTGGTTAAACCAAAAATATTTAaaaa                                                                                                              >  1:1513327/1‑128 (MQ=255)
                       gttttttgttttttATTATATTTTTTAAAATTTAAATGTTTTTAAATTT‑TTTTTTTTTTG‑G‑T‑T‑‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAAt                                                                                                          <  2:3871696/119‑1 (MQ=255)
                       gTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATTAAATTAAAA‑‑TTTTTAACAACAAAAACAAAATaaaaaaaa                                                                                                           >  2:962352/1‑128 (MQ=255)
                        ttCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGTACAACCAAAAATATATCaaaaaatt                                                                                                          >  1:3257002/1‑126 (MQ=255)
                         tCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAATAAAATTAATA‑‑ATTTGAACAACCAAAACTAAATaaaaaaaaat                                                                                                         >  2:3444672/1‑126 (MQ=255)
                         tCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTT‑T‑TAAAATAAAAGAAAGA‑‑TTTTGGTGAACCAAAACTAGTTCAAAAAAATTa                                                                                                        >  2:1702911/1‑128 (MQ=255)
                         catAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑TAAAATAAAATTAAGA‑‑ATTGGATAAAACAAAAAAAAATAAAAAAAAtt                                                                                                         >  2:3793225/3‑128 (MQ=255)
                         catAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑‑TTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGTAAAAAACAAAAATATATAAAAAAAATTa                                                                                                        >  1:2996216/3‑128 (MQ=255)
                          atttgatttttATTATAATTATCAAAATTAAAACGATTTAAAATTT‑TATTTTTTATG‑C‑T‑TT‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTa                                                                                                        <  1:419782/122‑1 (MQ=255)
                           tAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAAAAAAATTAATA‑‑TTTTTATAAAACAAAAATATATAAAAAAAAtata                                                                                                       >  1:1427320/1‑125 (MQ=255)
                             gtttttttATTTTAATTTTAAAAATTTCAATTCTTTAAAATTT‑TTTTTTTTTGG‑T‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTaaaa                                                                                                     <  2:1049967/125‑1 (MQ=255)
                              tttttttATTATAATTATTAAATTTAAATTGATTTTAAATTT‑TTTTTTTTTTG‑‑‑T‑T‑‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTaaaaaaa                                                                                                  <  2:2436734/126‑1 (MQ=255)
                              gttttttATTTTAATTATAAAAATTAAAACGCTTTAAAATTG‑TATTTTTTAGGTT‑T‑TT‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTaaaa                                                                                                     <  2:3501180/126‑1 (MQ=255)
                                      ttatACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTTAAAAAAAAAAATTATAA‑‑TTTGGTAAAACAAAAGATataaaaaaaaaataaaaaaaaaaatt                                                                                             >  1:168374/1‑124 (MQ=255)
                                      ttatACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTT‑T‑TAAAATAAAAGTAAGA‑‑TTTGTAAAAACAAAAAATATATAAAAAAAATTAAAAAAAAtagttt                                                                                           >  1:2771178/1‑128 (MQ=255)
                                       tttaCTTATAAAAATTAAAACGTTTTATAATTT‑TTTTTTTTTTG‑T‑T‑TG‑‑‑TTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAAtagttta                                                                                          <  2:1112694/126‑1 (MQ=255)
                                         taCTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGT‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAATAAAATTAATA‑‑TTTTGTAAAACCAAAAATTTTAAAAAAAAATTAAAAAAAAtattttat                                                                                         >  2:2998371/1‑127 (MQ=255)
                                         taCTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAATAAAATTAATA‑‑TTTTGATAAAACAAAAATATATAAAAAAAATTAAAAAAAAtattttag                                                                                         >  1:1627199/1‑128 (MQ=255)
                                         taCTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑‑TTTTTTTTTTTTTTTGT‑AAAAAAAAAAATAAGA‑‑TTTGGATCAACAAAAACTATAACAAAAAAATTAAAAAAAAtattttagt                                                                                        >  2:823944/1‑128 (MQ=255)
                                         taCTTATCAACATTACAACGCTTTCAAAGTG‑TATATTTTAGG‑C‑T‑TGT‑TTTTTTTTTTTTTTTTTT‑AAAAATAAAATTAATA‑‑TTTTGAACAACAAAAACTATAACAAAAAAAATAAAAAAAAtagttta                                                                                          >  2:3914370/1‑128 (MQ=255)
                                           ctttttataaTTATAACGCTTTAAAATTG‑TTTTTTTTAGT‑T‑T‑TT‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAAtagtttaggt                                                                                       <  1:2271646/119‑3 (MQ=255)
                                            ttATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAAAAAAATTAATA‑‑TTTGTATAAACAAAAAAATTATAAAAAAAATTaaaaaaaaaaattaatttt                                                                                      >  1:1305031/1‑117 (MQ=255)
                                             tATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TGTTTTTTTTTTTTTTTTTTTT‑AAAAATAAATATAAGT‑‑TTTTGATCAACCAAAAATTTATAAAAAAAATTAAAAAAAAtagtttattt                                                                                       >  2:3303120/1‑128 (MQ=255)
                                               taaaaaTTAAAAAGTTTTTAAATTG‑TTTTTTTTTTG‑G‑T‑TT‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGt                                                                                   <  2:1329489/123‑1 (MQ=255)
                                                  aCATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAATAAAATTAATA‑‑TTTGGAACAACCAAAACATTATAAAAAAAATTAAAAAAAATATTTTTGTTTTGTTTc                                                                                >  2:2053126/1‑128 (MQ=255)
                                                   cATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAATTAATA‑‑TTTTGATAAACAAAAACTATTAAAAAAAAATTAAAAAAAATTTTTTATTGTAGtttaa                                                                               >  1:2002006/1‑126 (MQ=255)
                                                   cATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAAAAAAAGTAAAA‑‑TTTGGATCAACCAAAACAATATCAAAAAAATTAAAAAAAATATTTTATTTgtttttga                                                                               >  2:355566/1‑121 (MQ=255)
                                                    aTTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑T‑‑‑TTTTTTTTTTTTTTTT‑A‑AAAAATAAAATAAAGA‑‑ATTTGATAAAAAAAAAAAATATAAAAAAAATTAAAAAAAAatgtatagttttttttaaata                                                                            >  1:769152/1‑117 (MQ=255)
                                                       aCAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TGT‑TTTTTTTTTTTTTTTTTT‑AAAAAAAAAATTAATA‑‑TTTGGATAAACCAAAAATATAAAAAAAAAATTAAAAAAAAAATTTTATTTTAGTTTCAAAt                                                                            >  2:3413265/1‑128 (MQ=255)
                                                            gCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TGTTTTTTTTTTTTTTTTTTTT‑AAAAACAAAATTAAGA‑‑TTTGGTACAACCAAAAAATTATAAAAAAAATTAAAAAAAATATTTTAGTTTAGTTTAAAATATGc                                                                        >  1:2057540/1‑128 (MQ=255)
                                                            gCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TGTTTTTTTTTTTTTTTTTTTT‑AAAAAAAAAATTAAGA‑‑TTTGGATCAACCAAAACATAATAAAAAAAATTAAAAAAAAAAGTTTATTTTAGTTTaaaaaaagc                                                                        >  1:2367207/1‑123 (MQ=255)
                                                             cTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TGT‑TTTTTTTTTTTTTTTTTT‑TAAAATTAAATTTATA‑‑TTTTGTTAAAACAAAAATATATAAAAAAAAATAAAAAAAAAATGTTATTTTAGTTTAAAAtataaat                                                                      >  2:3976840/1‑122 (MQ=255)
                                                             cTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAATAAAAGAAATA‑‑TTTTGATAAAACAAAACAAATAAAAAAAAATTAAAAAAAATATTTTATTTAAATTTCAAAAATGCata                                                                     >  2:3179503/1‑128 (MQ=255)
                                                               ttCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAAATAAATTAATA‑‑TTTGTATAAACCAAAAATATTTAAAAAAAATTTAAAAAAATTTTTTATTTTTGTTTGAAAtaatcaataa                                                                   >  1:83904/1‑119 (MQ=255)
                                                               ttCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAAAAAAATTAAGA‑‑TTTGGAAAAACCAAAAAAATTTAAAAAAAATTAAAAAAAATATTTTATTTTAATTTaaaaaaaaaatata                                                                   >  1:3422797/1‑118 (MQ=255)
                                                               ttCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑‑TTTTTTTTTTATTTTGT‑AAAAAAAAAAGTAAGA‑‑TTTTGATAAAACAAAACTATTTAAAAAAAATTAAAAAAAATATTTTAATTTAGTTTAAAAtattaaaatat                                                                  >  1:3568279/1‑118 (MQ=255)
                                                                tCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTT‑T‑AAAAATAAAAGTAATA‑‑TTTGGATAAAACAAAAATAAATAAAAAAAATTAAAAAAAATAATTGATTTGTGTTTAAAAAATGGAaatata                                                                 >  2:725312/1‑128 (MQ=255)
                                                                   atttttttttttttTGG‑T‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAg                                                                <  1:886795/120‑1 (MQ=255)
                                                                     gtg‑tATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGTTCAACCAAAACTATTTAAAAAAAATTAAAAAAAAAAGTTTATTTGAGTTTAAAATATGAATATATAGTAc                                                             >  1:483937/1‑128 (MQ=255)
                                                                         ttttttttttG‑T‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACaaa                                                          <  2:2002006/126‑1 (MQ=255)
                                                                         tATTTTTTAGG‑C‑T‑TGT‑TTTTTTTTTTTTTTTTTT‑AAAAATAAAAGTAAAA‑‑TTTGGAACAACCAAAACTAAAAAAAAAAAATTAAAAAAAATATTTTAGTTTATTTTAAAATATTAatataaattaaaa                                                           >  2:3693245/1‑120 (MQ=255)
                                                                          aTTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAATAAAATTAATA‑‑TTTGTATAAACAAAAAAAATATAAAAAAAATTAAAAAAAATATTTTATTTTAATTTAAAAACTGAAAATAAATTAaaaat                                                         >  1:3321266/1‑127 (MQ=255)
                                                                                 ttttttttttttttttttttttttttttGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAAAAAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGa                                                       <  1:314531/116‑1 (MQ=255)
                                                                                 aGG‑C‑T‑TGT‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAATA‑‑TTTGGATAAACAAAAAATATAAAAAAAAAAATAAAAAAAATATTTTAATTTAGTTTAAAATCTTAATATTTATTAAAAAGTAtttt                                                   >  2:2978349/1‑128 (MQ=255)
                                                                                 aGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAAAAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATAAAAAAAATTAAAAAAAATAGTTTATTTGAGTTTCAAATCTGAATATATATTACAAAGGAATTTa                                                  >  1:1834164/1‑128 (MQ=255)
                                                                                  gg‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTTT‑AAAAAAAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATTAGAAAAAAATTAAAAAAAATAGTTAATTTTATGTTCAAATCAGCATTATTAGTACTAAGTAATTTAt                                                 >  2:2252726/1‑128 (MQ=255)
                                                                                     ttttggttttttttttttttttttGT‑AAAAATAAAATTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCATATCTGCATATATAGTACAAAGGAAttt                                                   <  1:3638955/120‑1 (MQ=255)
                                                                                     ttgtttttttttttttttttttttGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAAttt                                                   <  1:3829208/120‑1 (MQ=255)
                                                                                     ttgcttttttttttttttttttttGT‑AAAAATAAATTTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACTAAGGAAttt                                                   <  2:453308/120‑1 (MQ=255)
                                                                                          ttttttttttttttttttttt‑AAAAATAAAAGTAAGA‑‑TTTGGATAAACAAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGAt                                              <  1:304733/125‑1 (MQ=255)
                                                                                          tttttttttttttttttttGT‑AAAAATAAAAGTAAGA‑‑TTTGGACCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGAt                                              <  1:1392123/125‑1 (MQ=255)
                                                                                            ttttttttttttttttttt‑AAAAATAAAAGTAAGA‑‑TTTTGATAAAACAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAg                                            <  2:490246/127‑1 (MQ=255)
                                                                                             tttttttttttttttttt‑AAAAATAAAAGTAATA‑‑TTTGGATAAACCAAAAATATATAAAAAAAATTAAAAAAAATATGTTTGTTGGGTTTCAAATATGCATATTATGTACAAAGCAATTtattattta                                           >  1:2581352/1‑125 (MQ=255)
                                                                                             ttttttttttttttttGT‑AAAAATAAATGTAAGA‑‑TTTGTATAAAACAAAAATATATAAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGa                                           <  2:1933972/128‑1 (MQ=255)
                                                                                               ttttttttttttttGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATAAAAAAAATTAAAAAAAATATTTTAGTTGAGTTTAAAATATGCATATATATTAAAAAGGAATTTAttttataaa                                         >  1:1337551/1‑120 (MQ=255)
                                                                                                    tttttttttGT‑TAAAATAAAAGTTAGA‑‑TTTGGATCATCCAATACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAAt                                    <  1:3186320/128‑1 (MQ=255)
                                                                                                    tttttttttGT‑AAAAATAAAAGTAAGA‑‑TTTGGTTCAACCAAAAATATTTAAAAAAAATTAAAAAAAATATTTTAGTTGAGTTTAAAATATGCAAATATAGTAAAAATGATTTTATGATATAAAAAAAt                                    >  1:1868797/1‑128 (MQ=255)
                                                                                                    tttttttttGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAAt                                    <  2:3354056/128‑1 (MQ=255)
                                                                                                      tttttttGT‑AATAATAAAAGTAAGA‑‑TTTGGATCAAACAAAAATATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATgg                                  <  1:1006887/128‑1 (MQ=255)
                                                                                                        tttttGT‑ATAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATTTAGTACAAAGGAATTTATGATAGAAAAAAATGGAt                                <  2:3140053/128‑1 (MQ=255)
                                                                                                         tttttt‑AATAATATAAGTAAGA‑‑TTTGTATCAACTAAAAATATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGAtt                               <  2:3748037/128‑1 (MQ=255)
                                                                                                           tttt‑AAAAATAAAATTAAGA‑‑TTTTGTTCAACCAAAAATATATAAAAAAAATTAAAAAAAATATTTTAGTTTAGTTTCAAATAAGAATATTTATTAAAAATGAATTTATTATATAAAAAAATGGATTAg                             >  1:3462726/4‑128 (MQ=255)
                                                                                                           ttGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAg                             <  2:3045930/128‑1 (MQ=255)
                                                                                                                aaataTAAAAGTAAGA‑‑TTTGTATAAAACAAAAATATATAAAAAAAATTAAAAAAAATAGTTTAGTTTAGTTTAAAATCTGCATATATATTACAAAGGAATTTATGATAGAAAAAAATGGATTatcaac                         <  1:2710891/128‑6 (MQ=255)
                                                                                                                     tAATAGTAAGA‑‑TTTGGATCAATTATATATATATTAAAAAAATTAAAAATTATAGTTTAGTTGAGTTTAAATTCTGAATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATc                    <  2:149825/128‑1 (MQ=255)
                                                                                                                     tAAAAGTAAGA‑‑TTTGTATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATc                    <  1:3691891/128‑1 (MQ=255)
                                                                                                                      aaaaGTAAGA‑‑TTTGGATAAACAAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTTAGTTTAAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATcc                   <  1:178958/128‑1 (MQ=255)
                                                                                                                        aaGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCAt                 >  1:3523924/1‑128 (MQ=255)
                                                                                                                         attAAGT‑‑TTTGTATAAAACAAATCTTTATTAAAATATTTAAATAAAATATTTTAGTTGAGTTTCATATCTGCTTATATATTACAAATGAATTTATTATAGAAAAAAATGGATTAGTTGATAATCCAtt                <  1:1199975/126‑1 (MQ=255)
                                                                                                                         aGTAAGA‑‑TTTTTATTAAAAAAAACTATATAAAAAATATTAAAAAAAATAGTTTAGTTTAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCAtt                <  1:2457363/128‑1 (MQ=255)
                                                                                                                          atgtaatttttGGATAAAACAAAAATATATCAAAAAAATTAAAAAAAATAGTTTTGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCAt                 <  2:2753944/120‑1 (MQ=255)
                                                                                                                            taGA‑‑TTTGTATTAACTAAAACTATATAAAAAAAATTAAAATATATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCAttttt             <  2:1400966/127‑1 (MQ=255)
                                                                                                                            taGA‑‑TTTGGATAATTTAATATTATATATAAAAAATTAAAAAAAATTGTTTTGTTGAGTTTCAAATCTGGATATATAGTAAAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCAttttt             <  2:2629137/127‑1 (MQ=255)
                                                                                                                            aata‑‑TTTGTTTCAACAAAAAATATATCAAAATAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCAttttt             <  2:1810414/125‑1 (MQ=255)
                                                                                                                            aaGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGAATAGTTGATAATCCAttttt             >  2:3087954/1‑128 (MQ=255)
                                                                                                                             aGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCAtttttt            >  2:618755/1‑128 (MQ=255)
                                                                                                                             aGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCAtttttt            >  1:965685/1‑128 (MQ=255)
                                                                                                                             aGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGAGGAGTAGATAATCCAttgtgt            >  1:2658375/1‑124 (MQ=255)
                                                                                                                             aGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGAATATATAGTACAAATGAATTTATGATAGAAAAAAATGTATTAGTATATAATACAtttttt            >  2:2860206/1‑128 (MQ=255)
                                                                                                                               a‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCATTTTTTaa          >  1:53244/1‑128 (MQ=255)
                                                                                                                               a‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCAATTTTTTat          >  1:676830/1‑127 (MQ=255)
                                                                                                                               a‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGATGAGAATCCATTTTTTaa          >  1:2530798/1‑128 (MQ=255)
                                                                                                                                   ttGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACATAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCATTATTTAAtt        >  1:111210/1‑128 (MQ=255)
                                                                                                                                   ttGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCATTTTTTAAtt        >  1:2190289/1‑128 (MQ=255)
                                                                                                                                   ttGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCATTTTTTAAtt        >  1:2835010/1‑128 (MQ=255)
                                                                                                                                   ttGGATAAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTATCAACTAATCCAtttttttcta        <  1:3900730/128‑5 (MQ=38)
                                                                                                                                      gATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCATTTTTTAATTTTg     >  2:1238284/1‑128 (MQ=255)
                                                                                                                                         |                                                                                                                               
AAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTG‑TATTTTTTAGG‑C‑T‑TG‑‑TTTTTTTTTTTTTTTTGT‑AAAAATAAAAGTAAGA‑‑TTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAAGGAATTTATGATAGAAAAAAATGGATTAGTTGATAATCCATTTTTTAATTTTGTTT  >  CP016816/203809‑204064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑