Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_tpiA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_tpiA2,817,1700CA97.4% 111.4 / NA 38E112* (GAA→TAA) yqaBfructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (20/17);  minor base T (1/0);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTT‑TCACGCGCTAACGCATGCGGGTCGAGATCGGC  >  NC_000913_3_vch_tpiA/2817024‑2817314
                                                                                                                                                  |                                                                                                                                                  
cGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGAACGGCAGCGACGACGGCGCCAAAATCATGGCGTAATCCCAGGTGCGCCCGCAATGCATCAGCGAT‑GCGCTTTTAcc                                                                                                                                                 >  2:186528/1‑148 (MQ=255)
   cGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTcc                                                                                                                                               <  2:820251/149‑1 (MQ=255)
          aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTcc                                                                                                                                        >  1:61995/1‑149 (MQ=255)
                    gCTGCGCGCACAACAAAAATGTGTCTGGGGCGGGTTTATGGGGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt                                                                                                                              <  2:540823/149‑1 (MQ=255)
                           gcACAACTAAATCGTCCCTGGCCCGGGTTTATGGTGTTTGACGTCATCGGCAGCTACGACGGCGTCAAAACAATGGCTTAATCCCAGCTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCTTTCCTACAGCCATTGGGcgac                                                                                                                       <  2:576934/149‑1 (MQ=255)
                           gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGCGTTCGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTACATCCCCGTTCCTACAACCATTgcgcacc                                                                                                                       >  2:165959/1‑146 (MQ=255)
                               aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAcc                                                                                                                   >  1:37128/1‑149 (MQ=255)
                               aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCAAGGTGCGCCAGCAATGACTCAGTGATGGCTATATAAATTCCCATTCCTACAGCCGTTTGGGGACGAcc                                                                                                                   >  2:796015/1‑149 (MQ=255)
                                 caaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTCCAGCCCTTGGGCGACCACCAt                                                                                                                 >  2:707531/1‑149 (MQ=255)
                                 caaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                 >  1:674689/1‑149 (MQ=255)
                                 aaaaaaTGTCTCTGGTGTGGGTGTATGGTGGTGCACGTGATCCGCAGCGACGCAGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGTAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGTCATTGGGCGACGACCAt                                                                                                                 <  2:234255/148‑1 (MQ=255)
                                  aaaaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATg                                                                                                                >  1:693266/1‑149 (MQ=255)
                                   caactgtgTCTGGCGTGGGTTTATGGTGTTTCACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCTCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGc                                                                                                               <  1:347936/145‑1 (MQ=255)
                                       tgtgGCAGGGGCGGGTTTTTGTTGTTTGACGTGATCGGCAGCGACGACGCTGTTACGATATTGGCGTAATCTTACCCGCGACAGCAATGCCCCGGCGATGGCGCTTTACCTCCCCGTTCCTACAGCCCTTGGGCGACGACCATGCTAAc                                                                                                           <  2:91869/149‑1 (MQ=255)
                                           tCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCCTGCCAACtttt                                                                                                       >  1:53717/1‑149 (MQ=255)
                                                cgcgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcacca                                                                                                  <  1:672386/149‑1 (MQ=255)
                                                   gggTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACAt                                                                                               <  1:609487/149‑1 (MQ=255)
                                                    ggTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCATGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATc                                                                                              <  2:502719/149‑1 (MQ=255)
                                                       ttATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATcaac                                                                                           <  2:425574/149‑1 (MQ=255)
                                                          tGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCACTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACCag                                                                                        >  1:117838/1‑149 (MQ=255)
                                                            gtgtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAagag                                                                                      >  2:696305/1‑149 (MQ=255)
                                                            gtggTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAGTGGGCGACGACCATGCCAACTTTTCACCACATCAACAagag                                                                                      <  2:470618/149‑1 (MQ=255)
                                                                        aTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCCCCACATCAACAAGAGGAAGCGGTTCG‑a                                                                         >  2:320209/1‑149 (MQ=255)
                                                                                    gagggcggcAAAACAATGGCGTAATCCCAGGTGCGCCAGCAATGCCGCAGCGATGGCGCTTTATCTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACGTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCcagca                                                             <  2:38249/146‑1 (MQ=255)
                                                                                    gacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCcagca                                                             <  1:119945/149‑1 (MQ=255)
                                                                                                       cGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTCTTACTGCTTc                                          >  2:181143/1‑149 (MQ=255)
                                                                                                         tAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTCTTACTGCTTCTg                                        >  1:754387/1‑149 (MQ=255)
                                                                                                                       ccAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTAAGTATGATAT‑AAGCAGCATACTGATTACTGCTTCTGTTTTT‑TCACGCGCt                         >  1:191755/1‑149 (MQ=255)
                                                                                                                          gCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTT‑TCACGCGCTAAc                      <  1:696305/149‑1 (MQ=255)
                                                                                                                                ccTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTT‑TCACGCGCTAACGCATGc                >  1:4039/1‑149 (MQ=255)
                                                                                                                                 ctgaGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCTACTTTTCACCACATCACCACTAGTAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTTTTTCTGCTTCTGTTTTT‑TCACGCGCTAACGCCTGcc               >  2:575075/4‑148 (MQ=255)
                                                                                                                                  tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTT‑TCACGCGCTAACGCATGCgg              >  1:798132/1‑149 (MQ=255)
                                                                                                                                  tCAGCGAAGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAGGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTT‑TCACGCGCTAACGCATGCgg              <  2:53983/149‑1 (MQ=255)
                                                                                                                                    aGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATCCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTT‑TCACGCGCTAACGCATGCGGGt            >  2:138859/1‑149 (MQ=255)
                                                                                                                                        atagcgcTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAAGATGCCAACTTTTCAGCACATCAACAAGAGGAAGCGGTTCG‑ACCCTATACAGCCGCATACCTCTTCCTGCTTCTGTTTTTCTCAC‑CGCTAAACAATGCGGGGCgcg        >  2:65253/4‑147 (MQ=255)
                                                                                                                                           gcgcTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCcagcag                                                            >  1:524412/1‑95 (MQ=255)
                                                                                                                                           gcgcTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCcagcag                                                            <  2:524412/95‑1 (MQ=255)
                                                                                                                                              cTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTT‑TCACGCGCTAACGCATGCGGGTCGAGATCGGc  <  2:742607/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCG‑ACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTT‑TCACGCGCTAACGCATGCGGGTCGAGATCGGC  >  NC_000913_3_vch_tpiA/2817024‑2817314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑