Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	482,230	G→A	P725L (CCG→CTG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	482,230	0	G	A	100.0%	69.4 / NA	23	P725L (CCG→CTG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

ATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGC  >  NC_000913_3_vch_tpiA/482088‑482368                                                                                                                                               |                                                                                                                                           atAAACTTCCTTCACACGCCCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTAt                                                                                                                                      <  2:58283/149‑1 (MQ=255)     acgttcttcCAACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                  <  2:449907/146‑1 (MQ=255)                acGACCGCGGTCTATAAAGTTGTTCACATCGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAATCACCTATCGCCTTTGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTg                                                                                                                       <  1:43242/149‑1 (MQ=255)                acGACCGCGGTCGATAAAATCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGAGGTTAATGTAGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTAACTCCCAGACCGttag                                                                                                                       >  2:153780/1‑147 (MQ=255)                   gccgccgTCGATGAAGTCGGTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGGTGATAGAAACCCCCAGCGCCGGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGAc                                                                                                                    <  2:493147/148‑1 (MQ=255)                                           aTAGCCGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGATACACCCAGCGCCTGCTCTTTTTCCTGGTTGATATAAATTTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                            <  1:238458/149‑1 (MQ=255)                                             aGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTACTGTCGTTGATAGAAACACCCAGCGCCAGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCGGGTCAACATATCAGGGTGc                                                                                          >  1:17469/1‑149 (MQ=255)                                             aGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAAACACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGc                                                                                          >  1:293544/1‑149 (MQ=255)                                                         aTGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGTTATCTTTTAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCt                                                                              <  1:343932/149‑1 (MQ=255)                                                                          gtgtTAATGTCATTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTACAGGGTATCTTCCAGACCGGTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcg                                                             >  1:337712/1‑149 (MQ=255)                                                                           tgtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTACTGGTCGATATCAATCTTAAACTGCAGAGTATCTTCCAGACCGTTTGGACATAAGCTGGTCAACATATCAGGGGGCTTCGCGGCTTCTGCAAGCAACTGGTTAcgc                                                            >  1:23478/1‑149 (MQ=255)                                                                                      ttGATAGAAACACCCAGCGCCTGCACGTGTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGATTCGCTGCTTCTGCAAGCAACTGGTTACGTGCCTGAGTCAg                                                 <  2:252205/149‑1 (MQ=255)                                                                                            gAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                           <  1:52900/149‑1 (MQ=255)                                                                                              aaCCCCCAGCGCCTGCGCATTTTCATGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                         <  2:433172/149‑1 (MQ=255)                                                                                                   ccAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTAGACGTACGCTGGTCAACATATCAGGGTACTTCCCTGATACTGCAAGCAACGGGTTAGGGGACTGAGTAAGCATTTCGAGAACa                                    >  1:390801/1‑149 (MQ=255)                                                                                                        gcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGTTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGcc                               <  1:440816/149‑1 (MQ=255)                                                                                                               cTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGt                                                    <  2:321649/121‑1 (MQ=255)                                                                                                               cTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGt                                                    >  1:321649/1‑121 (MQ=255)                                                                                                                                  aaTCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa     >  1:398857/1‑149 (MQ=255)                                                                                                                                    tCTTAAACTGCAGGGTATCTTCCAGCCCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag   >  1:174657/1‑149 (MQ=255)                                                                                                                                    tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag   >  2:307910/1‑149 (MQ=255)                                                                                                                                    tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCATCGCTACTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag   >  1:67510/1‑149 (MQ=255)                                                                                                                                     cTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc  <  1:307910/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                           ATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGC  >  NC_000913_3_vch_tpiA/482088‑482368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑