| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 2,214,152 | A→T | Y165N (TAT→AAT) | yehU ← | inner membrane putative sensory kinase in two‑component system with YehT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 2,214,152 | 0 | A | T | 96.0% | 71.9 / ‑5.6 | 25 | Y165N (TAT→AAT) | yehU | inner membrane putative sensory kinase in two‑component system with YehT |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (10/14); minor base G (1/0); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTT > NC_000913_3_vch_tpiA/2214016‑2214295 | gCAGAAAAAGCCTAAGTGTATTTTTCAATCATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGAt < 2:419066/149‑1 (MQ=255) cAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATAAACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATc < 2:105051/149‑1 (MQ=255) gAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGgatgat > 2:402969/1‑149 (MQ=255) aaGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATc < 2:286509/149‑1 (MQ=255) tCAAACCTCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTTCTCTCCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCAc < 1:364837/149‑1 (MQ=255) tCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCAc < 2:76215/149‑1 (MQ=255) aaaCATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGAGGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCa > 2:418883/1‑149 (MQ=255) tATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTTATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAAc < 1:459745/149‑1 (MQ=255) atatACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGcc > 2:156598/1‑148 (MQ=255) gCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTCCTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACAAACGTGACGGCACcggag > 1:346963/1‑147 (MQ=255) cATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACcggcgg < 1:418883/149‑1 (MQ=255) aCAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGTGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATg < 2:41419/149‑1 (MQ=255) aGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATggg < 1:412684/149‑1 (MQ=255) gccgACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGTTTCTTTGCGGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGa < 1:355017/149‑1 (MQ=255) gccgACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGa < 1:307965/149‑1 (MQ=255) gTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTgcg > 2:64232/1‑149 (MQ=255) gTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGAACCGAAAAGAGTTGTGTCGGGCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTCATGGGGTTAAAGACGTTATACGTgcg > 2:505959/1‑149 (MQ=255) tGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTACAGACCTTATCAGTgcgc > 2:500964/1‑149 (MQ=255) tcatTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCgcgg > 1:460159/1‑149 (MQ=255) gcaATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGAt < 1:64232/149‑1 (MQ=255) gcaATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGAt < 1:402969/149‑1 (MQ=255) cACCAGACGCACCGCCTCTTCATTAGGTCGGGCGATCGCAAGGATGACCATCAGTTGCACCATTTCAGCGACGAACGTGACGGCACCGTCGGTAATGGGGTTAAAGACTTGATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCa < 2:97693/149‑1 (MQ=255) aCCAGACGCACCGCATCTTCATTAGGTCCGGCGATCGCAAGGATGATCAGCATTTGAACCATTTCAGCGACGAACGTGACGGCCCCGGCGGTAACGGGGTTAAAGAATTTAACATTCCGCCCGCGGCGCATCAGGAAGCTgcgttacag > 2:86784/1‑144 (MQ=255) gCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCAcag > 1:301483/1‑147 (MQ=255) ttCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCtt > 2:334553/1‑149 (MQ=255) | GCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTT > NC_000913_3_vch_tpiA/2214016‑2214295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |