Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_tpiA 3,815,810 Δ1 bp intergenic (‑42/+24) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_tpiA3,815,8090G.97.1% 144.0 / ‑3.2 35intergenic (‑41/+25)pyrE/rphorotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (14/20);  minor base A (0/1);  total (14/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACT  >  NC_000913_3_vch_tpiA/3815664‑3815953
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gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAgcga                                                                                                                                               <  1:379867/149‑4 (MQ=255)
          gtgCTTTTGTGCGTGTATTTCAGCGTAAACGCGCCAAACTTTCACACCTGCTTGCTAAGCGTAAATTCAATAAACTGGCGCTTATATGGTTTCATGCCTTTGCGCCTCATCTTATTTTTATACAGACAAAAAAAAGCGACTCATCAGTc                                                                                                                                    <  2:270946/147‑1 (MQ=255)
                     cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCCACCCATCTGCCGCCGTAAAAAt                                                                                                                         >  1:286205/1‑149 (MQ=255)
                      ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTTCTTGCTAAGCGCAAATTCAATAAACTGGAGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTCAAAATc                                                                                                                        >  1:35554/1‑149 (MQ=255)
                      ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTTTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAATAATc                                                                                                                        >  1:42570/1‑149 (MQ=255)
                           tttCAGCGTAAACTCGCCAAACTTTAACACCTTTTTGCTAAGCGCAAATTTAATTATCTGGCGCTTATATGGTTTTATGCCTTCGCTTCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGttt                                                                                                                   <  1:234297/149‑1 (MQ=255)
                               aGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCa                                                                                                               <  1:90634/149‑1 (MQ=255)
                                  gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCGGTTTGCCAgcc                                                                                                            >  2:153436/1‑148 (MQ=255)
                                      aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAACCTGGCGCTGATATGATTTAATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCGTAAAAATCAGTTTGCCACcgccgc                                                                                                        >  1:68530/1‑149 (MQ=255)
                                      aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccgc                                                                                                        >  1:55636/1‑149 (MQ=255)
                                             aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                                                                                                 <  1:463519/149‑1 (MQ=255)
                                             aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAAATGGCGCTGATATGGTTTCATGGCTTCGCTCCTCATCTTACTTTTCTACAGACATAAAAAAGAGACTCATCCATTGCCTTAAAATTCAGTTTGCAAGCGCCGCCTTTTGc                                                                                                 >  2:235907/1‑149 (MQ=255)
                                                cggtAACACCTGCTTGCTAAGCGCAAATTCAAGAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc                                                                                              <  2:42570/146‑1 (MQ=255)
                                                 ttcaaCACCTGCTTGCTAAGCACAAATACAATACACTGACGCTAACAAGGGAACAGGCCGTTTCTCCTCATCTTACTTTTCTACAGACAAAAAAAACCGACACATAAGCCGCCCTCACACTCAGCTTCCCACCCCCCCCCCCTGCCTCg                                                                                             >  2:229477/4‑149 (MQ=255)
                                                         cTGCTTGCTCAGCGCAAATGCAATAAACTGGCTCTGATTTGGTTTCATGCCTTCTCTCCTCATCTTTCTTTTCTACATACAATTAATATTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATg                                                                                     <  1:30767/149‑1 (MQ=255)
                                                            cttgctAAGCGCAAATTCAATAATCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat                                                                                  <  1:443425/149‑1 (MQ=255)
                                                               gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGCCAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCCGCGCCGCCTTCTGCGTACCTACAATGgcttcc                                                                               >  2:8188/1‑148 (MQ=255)
                                                                ctagtcgcACATTCAATTAACTGTCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACATACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                              <  1:261146/144‑1 (MQ=255)
                                                                ctACGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                              <  1:153436/149‑1 (MQ=255)
                                                                ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                              <  1:187702/149‑1 (MQ=255)
                                                                 tAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCTCCGCCTTCTGGGCCCCTACAATGgatccgat                                                                             >  2:45849/1‑149 (MQ=255)
                                                                                tAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCGGCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga                                                              <  1:494133/149‑1 (MQ=255)
                                                                                          cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCTAGCGCCGCTTTCTGCCGCTCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGa                                                    <  1:545265/149‑1 (MQ=255)
                                                                                               atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGt                                               <  1:441390/149‑1 (MQ=255)
                                                                                                    ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGctc                                          <  1:51904/149‑1 (MQ=255)
                                                                                                         gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt                                     <  2:55636/149‑1 (MQ=255)
                                                                                                           cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCTTTAAAAATTTGTTTGCCAGCGCCGTCTTCTGCGTGGTTACGATGGATTCGATTCCCCGCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                   <  1:120677/149‑1 (MQ=255)
                                                                                                           cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                   <  1:397937/149‑1 (MQ=255)
                                                                                                           cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                   >  2:33631/1‑149 (MQ=255)
                                                                                                           cTTCGCTCCTCATCCTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTTGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                   <  1:448236/149‑1 (MQ=255)
                                                                                                            ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat                                                                                  >  2:358083/1‑101 (MQ=255)
                                                                                                            ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat                                                                                  <  1:358083/101‑1 (MQ=255)
                                                                                                                         ttACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGTGAAGTCTCCTACGTCGCTACAATGGATTCGATTCCCCTCGGGCCAAAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                     >  1:104088/1‑149 (MQ=255)
                                                                                                                             ttttCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTTGATTTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCt                 <  1:348801/149‑1 (MQ=255)
                                                                                                                             ttttCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAAAAAGATAGGTAGCTCTTCATGGGGGAACGGCTCGCCTTCt                 >  2:216883/1‑149 (MQ=255)
                                                                                                                                             aaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACt  <  2:286205/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACT  >  NC_000913_3_vch_tpiA/3815664‑3815953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: