Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	1,196,232	C→T	T370T (ACC→ACT)	icd →	isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	1,196,232	0	C	T	100.0%	34.1 / NA	15	T370T (ACC→ACT)	icd	isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/8);  total (7/8)

GCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGG  >  NC_000913_3_vch_tpiA/1196089‑1196314                                                                                                                                                |                                                                                   gCCCCTGGTGCAAACATCGGTGACGCATACCAACGCGTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACCAAGTAAATCCTGGCTCAATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGACCTGAAGc                                                                               >  2:425103/1‑149 (MQ=255)         tGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcc                                                                       >  1:217537/1‑148 (MQ=255)              aCATCGGTGACGAATGGGCCCTGTTTGAAGGCACGCACGGTACTGCGCCGAAATATGCAGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAtt                                                                  <  2:395268/149‑1 (MQ=38)                aTCAGTAACGAATAACCCCTGTTTGAAGCCACCCACGGTACTGCGCCAAAATAAGCCGCTCAGGACAAAGTAAATCCAGGCTCTATTATTCCCTCCGCTGAGATGATGCTGCGCCATATGGGTTAGACTGAAGCGGCTGACCTGATTGt                                                                <  2:10302/149‑1 (MQ=25)                   ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCACCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaa                                                             >  2:34650/1‑149 (MQ=38)                    gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                            >  1:374739/1‑149 (MQ=255)                    gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                            <  2:217537/149‑1 (MQ=255)                                 ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                               >  1:369807/1‑149 (MQ=255)                                         aaGCCAACCACGGTAATGCGCCGAAATATGCCGGCCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                       <  2:196012/149‑2 (MQ=25)                                           gCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATTATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTGAAAGGTATGGAAGGCGCAATCAAtgc                                     <  1:34650/149‑1 (MQ=25)                                              acccacGGTACTGCGCCGAAATATGCCGGTCACGACAAAGTTAATCCTGGCTGTATTATGCGCTCCGTTGAGATGATGGTGCGCCAAATGGTTTGGACTGAAGCGGCTGACCTGATTCTTAAAGGTATGGAAGGCGACATCAATGCCaa                                  <  2:297459/149‑1 (MQ=18)                                                                       ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa                                                    >  1:434246/1‑106 (MQ=21)                                                                       ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa                                                    <  2:434246/106‑1 (MQ=21)                                                                           tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga     <  2:374739/149‑1 (MQ=14)                                                                              ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAGCGTCTgatgg  >  1:117475/1‑149 (MQ=14)                                                                                                                                                |                                                                                   GCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGG  >  NC_000913_3_vch_tpiA/1196089‑1196314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑