| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 4,604,746 | C→A | A186E (GCA→GAA) | bglJ → | bgl operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 4,604,746 | 0 | C | A | 96.2% | 69.3 / NA | 27 | A186E (GCA→GAA) | bglJ | bgl operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (15/10); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGG > NC_000913_3_vch_tpiA/4604603‑4604882 | cgaccgacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGGGAGAGAAATATTGCGCTTTATGTCGCGTGGCTATTCAATGACACACAGTGCCGAGCAGCTTAAATGCAATATCAAAACGATCCGTGaaccaa > 1:161836/1‑146 (MQ=255) acTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTa > 1:300250/1‑149 (MQ=255) tGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAAt > 1:309676/1‑149 (MQ=255) tGAACAATCAGTGGTACATACACCAACGCCACACGTAAACCCCGCAGGTCAAAGAATTATAACGAATCAATTCGCGTGGCTACTCAATGTCACAAATTGCCGAGCAGCTTAACCTCAAAACCAAAACAATCCGTGGACATAAATTTAAt > 2:86843/1‑149 (MQ=255) aaCAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGATAGAAATATTGGTCATTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACACAATATCAAAAAGATCCGTGAACAAAAATTTAAtgt > 1:324342/1‑149 (MQ=255) aCATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAAAATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTg > 1:138554/1‑149 (MQ=255) aCATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACACAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTg > 1:232208/1‑149 (MQ=255) cAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAATCGAGAACATAAATTTAATGAGATACCGAACCTGCGCGTCAg > 2:71340/1‑149 (MQ=255) tAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGAAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg < 1:83322/149‑1 (MQ=255) cccGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgt > 1:141519/1‑149 (MQ=255) cccGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgt > 1:400928/1‑149 (MQ=255) aCGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGATACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa < 1:53346/149‑1 (MQ=255) agagagaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGcc > 1:17468/1‑149 (MQ=255) gagaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAGCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCa < 2:103090/149‑1 (MQ=255) gcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTta < 2:17468/149‑1 (MQ=255) gagCTACTCAATGACACAAATTGCCGATCAGCTTAAACGCAATATAAAAACGATCCGTGAACATAAATTTAATTGAATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCAtt < 1:404258/147‑1 (MQ=255) acTACTCAATAACACAAATTGGCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAAGTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGc < 1:75071/148‑1 (MQ=255) tACTCAAGGACACAAATTGCCGAGCAGTTTAAACACAATATCAAAACGATCCGTGAACATAAATTTAGTGTGATGTCGAAACTGGGCGCCAGTTCTGACGCAGTGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGa < 2:324342/149‑1 (MQ=255) tcacacaTTTCCGCGCAGTTTAAACTCAAGATCAAGACGATCCGTGAATATAAATTTAATGTGATGTCGAAACGGGGCGGCAGTGCTGTCGCAGGGTTGTTTGAGGCTGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTaa < 2:232208/148‑1 (MQ=255) aTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGtt < 2:300173/149‑1 (MQ=255) tGCCGAGCAGCTTAAAGGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGc > 1:383593/1‑149 (MQ=255) gAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGATGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAGGTAATGTGTTGCATcc > 1:272472/1‑149 (MQ=255) cTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTACTGTGTTGCATCCCTATTa > 2:120737/1‑149 (MQ=255) ttAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTaa > 2:198452/1‑149 (MQ=255) gCAATATCAAATTGATTCGTGAACATAAACTCGATGTTATGACAAAACGGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCa < 1:80664/149‑1 (MQ=255) aaTATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTATGATATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTCATCCGAatg > 1:109867/1‑149 (MQ=255) tCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGc > 2:253341/1‑149 (MQ=255) aaaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTAGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATGGCGAAACAAGGAATGTGTTGCATCCCTATTAATCCGCCTTATGCCgg > 2:156657/1‑149 (MQ=255) | CGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGG > NC_000913_3_vch_tpiA/4604603‑4604882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |