Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	2,111,423	G→T	A185E (GCG→GAG)	rfbD ←	dTDP‑L‑rhamnose synthase, NAD(P)‑dependent dTDP‑4‑dehydrorhamnose reductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	2,111,423	0	G	T	100.0%	76.3 / NA	26	A185E (GCG→GAG)	rfbD	dTDP‑L‑rhamnose synthase, NAD(P)‑dependent dTDP‑4‑dehydrorhamnose reductase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/10);  total (16/10)

TCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTT  >  NC_000913_3_vch_tpiA/2111282‑2111567                                                                                                                                              |                                                                                                                                                 tCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTCGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACGCGAATGGCATGTGCTGTACAATCCGCCAGCAGTTCAGCACCTGTTGGCTCACCaaa                                                                                                                                           <  2:245837/149‑1 (MQ=255)  cAAAAACCGGCGCAGCATAATCGTACCAGGTGGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAAc                                                                                                                                          <  2:337969/149‑1 (MQ=255)      atccAGCGTAGCATAATAGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGAt                                                                                                                                      <  2:97451/147‑1 (MQ=255)          aGCGCAGCATAATCGGACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGtt                                                                                                                                  <  1:106235/149‑1 (MQ=255)                      tCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCCCTaa                                                                                                                      >  2:385581/1‑149 (MQ=255)                                  gtgGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGAGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGctc                                                                                                          >  1:286478/1‑149 (MQ=255)                                        ccACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTCATAACCGCTAATTCTTCACGCTCTTTTGc                                                                                                    >  2:228997/1‑149 (MQ=255)                                         cACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCCTGTCCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTAcc                                                                                                   >  1:83364/1‑149 (MQ=255)                                            tGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACAGCTAATTCTTCCCCCTCTTTTGCCAGa                                                                                                >  2:24258/1‑149 (MQ=255)                                                tACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTAATCGTTAATAACCGCTAATACTTCACGCTCTTTTGCCCGACGTa                                                                                            >  1:95294/1‑149 (MQ=255)                                                          tACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTAACGCTCTTTTGCCAGACGTAACATCGtttt                                                                                  >  1:356949/1‑149 (MQ=255)                                                           aCAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTg                                                                                 <  1:255751/149‑1 (MQ=255)                                                            cAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGa                                                                                                <  2:264850/133‑1 (MQ=255)                                                            cAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGa                                                                                                >  1:264850/1‑133 (MQ=255)                                                                ccTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTATTTTGCCAGACGTAACATCGTTTTGGCgca                                                                            >  1:120907/1‑147 (MQ=255)                                                                           cGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTAtttcctt                                                                 >  1:204480/1‑149 (MQ=255)                                                                                     aGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATa                                                       <  2:204480/149‑1 (MQ=255)                                                                                         cGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGATCACAAAACTGATAGTTAATAACCGCTAATTCTTCAAGCTGTTTTGCAAGACGTAAAATCGTTTTGAGCAAGGTATTTCCTTTTCCTGCAGAGAcg                                                   >  2:161160/1‑148 (MQ=255)                                                                                         cGACACGAATGGCATGTGCTCTACAATTATCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGAcc                                                   <  1:143395/149‑1 (MQ=255)                                                                                                              tACAATCAGCCAGCAGTTCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATCTACCCAGCTGTTCCGGAAAATAAgt                              >  2:275270/1‑148 (MQ=255)                                                                                                              tACAATCAGACAGCAGTTCAGCAACTGTTGGATCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGa                              <  1:179753/149‑1 (MQ=255)                                                                                                                 aaTCAGCCAGCAGTTCAGCACCTGTTGGCTCTATTTACTAATCGTGAATTTCCGCTAATTCTTCACGCTCTTTTGCCAGACGTAATATCGTTTTGGCGAAGTTATTTCTTTTTCCTTCTTAGTCGCAGGTTGTCGGGAAACTGAGAttc                           >  2:315014/1‑147 (MQ=255)                                                                                                                              ttCAGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATAtt              >  2:101677/1‑149 (MQ=255)                                                                                                                                 aGCACCTGTTGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCt           <  2:417958/149‑1 (MQ=255)                                                                                                                                   cACCTGTTCGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGTTCCGGAAAATAAGATGCTTCGCGCAATATTACTGt         >  2:122840/1‑149 (MQ=255)                                                                                                                                          tGGCTCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCtt  >  1:138757/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                 TCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTT  >  NC_000913_3_vch_tpiA/2111282‑2111567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑