Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	482,230	G→A	P725L (CCG→CTG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	482,230	0	G	A	100.0%	101.6 / NA	32	P725L (CCG→CTG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/12);  total (20/12)

TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGT  >  NC_000913_3_vch_tpiA/482084‑482372                                                                                                                                                   |                                                                                                                                               tgacatAAACTTTCTTCACACGACCGCGGACGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCagg                                                                                                                                              <  1:428853/149‑1 (MQ=255) tgacatAAACTTTCTACACACGACCGCGGTCGAGAAAGTCGTTCACATAGCTGCCGCCCCATTCAGCGCCCAGAGCGGTGTTAATGTCGTTGAGAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCagg                                                                                                                                              <  1:178722/149‑1 (MQ=255)     atAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTAt                                                                                                                                          >  1:334651/1‑149 (MQ=255)                tcACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCg                                                                                                                               >  2:462080/1‑149 (MQ=255)                   cacGACCGCGGTCGATAACGTCGTTCAAATTGCTGCCGCCCCATGCAGCGCCCGGAGTGGTGTTAATGTCGTTGATAGAAAAACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                            <  2:134425/149‑1 (MQ=255)                    acGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAAGGTGGTGTGAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTg                                                                                                                           >  1:471213/1‑149 (MQ=255)                                gATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGTTATCTTCCAGACCGTTTGGACGTACGCTgg                                                                                                               >  1:329162/1‑149 (MQ=255)                                 aTAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGGATCTTGCAGACCGTTTGGACGTACGCTGGt                                                                                                              >  1:215008/1‑149 (MQ=255)                                               aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                                <  1:306329/149‑1 (MQ=255)                                                              tGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTg                                                                                 <  1:482765/149‑1 (MQ=255)                                                                cAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGGCAACATATAAGGTTGATTCGCTGCATCTgcc                                                                               >  2:15885/1‑148 (MQ=255)                                                                cAGCGCCCAGAGAGGTGTTAATGTTGTAGATAGAAACACCCAGCGCCTGCGCTTTTTCGTGGTCGATATCAATGTTAAACTGCAGGGTGTCTTCCAGCCCGTTTGGACGTACGCTGGTCAAAAGATCAGGGTGCTGCGCCGCTTCTgca                                                                               >  2:311213/1‑149 (MQ=255)                                                                           gtggggTTAATGGAGGTGATAGAAACACCCAGCGCCTGCGCGTTTCCCTGGTCGATATCAATCTTAAACTGCAGGGTATCCTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCCCTGCTTCTGCAAGCAACTGGtt                                                                    <  1:61860/149‑1 (MQ=255)                                                                                gtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgcg                                                               >  1:128930/1‑149 (MQ=255)                                                                                       tCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGCCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACGGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTCCGCGCCTGAgc                                                        >  2:18986/1‑148 (MQ=255)                                                                                         gTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa                                                      <  1:377610/149‑1 (MQ=255)                                                                                         gTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa                                                      >  1:197667/1‑149 (MQ=255)                                                                                              tAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCACGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCAGCTTCTGCAAGCAACTGGTTACGCGCCTGATTCAGtttc                                                 >  2:27955/1‑148 (MQ=255)                                                                                                  aaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                             <  2:484633/149‑1 (MQ=255)                                                                                                  aaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATAAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCACCATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                             >  2:262196/1‑149 (MQ=255)                                                                                                    cacCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGa                                           <  2:128930/149‑1 (MQ=255)                                                                                                     acCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAc                                          >  2:249268/1‑149 (MQ=255)                                                                                                       ccAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGCCCa                                        >  1:22701/1‑149 (MQ=255)                                                                                                        cAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCaa                                       >  1:383099/1‑149 (MQ=255)                                                                                                         aGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                                      <  1:253215/149‑1 (MQ=255)                                                                                                                           tGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                    >  1:305544/1‑149 (MQ=255)                                                                                                                           tGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                    <  1:249268/149‑1 (MQ=255)                                                                                                                                   atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAAc                                                                                                          >  2:188106/1‑55 (MQ=255)                                                                                                                                   atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAAc                                                                                                          <  1:188106/55‑1 (MQ=255)                                                                                                                                    tCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc           <  2:383099/149‑1 (MQ=255)                                                                                                                                        tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCCGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGACTGAGTCAGTTTTTCGTGACCAAGGCAAGCCTGGTCAATCAGCtcaaagtcaaag       >  2:323078/1‑149 (MQ=255)                                                                                                                                         cTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc      >  1:21189/1‑149 (MQ=255)                                                                                                                                           tAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCg    <  1:426172/149‑1 (MQ=255)                                                                                                                                             aaCTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt  >  2:153650/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                               TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGT  >  NC_000913_3_vch_tpiA/482084‑482372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑