Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	264,098	0	G	T	100.0%	53.9 / NA	19	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/7);  total (12/7)

TAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAT  >  NC_000913_3_vch_tpiA/263971‑264238                                                                                                                                |                                                                                                                                             tAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGa                                                                                                                         >  1:395154/1‑149 (MQ=255)      ttctgCTGCAGGTCCTTGTTAGGGGTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTc                                                                                                                    <  2:98247/145‑1 (MQ=37)      tcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTc                                                                                                                    <  1:452867/149‑1 (MQ=255)               aGGCCCCGGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                                                                                                           <  2:302577/149‑1 (MQ=255)                 gCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                                                                                                         >  1:378050/1‑149 (MQ=255)                 gCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGCTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGTCACGTCCCTGCCGctgt                                                                                                         >  2:308240/1‑149 (MQ=255)                      tGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGa                                                                                                    <  1:446902/149‑1 (MQ=255)                        gTAGGGTTGGTTTTTTTAGTTTCCCTCTATGGGCAGTGCAGGGTTATGCTCATGAGGCAGGTATAGGGCTCATTGCGGCCCTGGGGGCGTTGGGGGTATACCGTTAGGTCTCCGGCGATATCCGGAACGTCCCTGTCGGTGTAATGata                                                                                                  <  2:132801/149‑3 (MQ=255)                                                           gTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGATATTTGCCGGTGCAGt                                                               >  2:219120/1‑149 (MQ=255)                                                                        cTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCg                                                  >  1:46387/1‑149 (MQ=255)                                                                        cTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCg                                                  <  2:46387/149‑1 (MQ=255)                                                                         tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                                 >  1:290941/1‑149 (MQ=255)                                                                           atcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATAACGCTGGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTCCCGTGCCACTGGTATTTGCCGGTGCAGTAGCGCAACATTCGGGa                                               >  2:210736/1‑149 (MQ=21)                                                                            tcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTCCAGGGGTTTCATTAGCTTCTACCGTGCCACTGGTCTTTGACGGTGCGGTAGCACAAGATTCGGGAc                                              >  1:117200/1‑149 (MQ=255)                                                                                         gggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGc                                 >  2:358557/1‑149 (MQ=255)                                                                                                     gTCCTGGCAGCGTTCGGCATATAACGCTAGGACTCCGGCGATATCCGGAACGTCGCTGCCGGTGTAATGAAAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGAGACTGGCGATACATCGACa                     >  2:324090/1‑149 (MQ=17)                                                                                                         tGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGc                 >  1:45198/1‑149 (MQ=255)                                                                                                                    ggCGTAAACCGCTAGGACTCCGGCGAGATCCGGAACGTCCCTGCTGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTAc      <  2:27911/149‑1 (MQ=255)                                                                                                                        tataCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAt  >  1:455364/1‑149 (MQ=255)                                                                                                                                |                                                                                                                                             TAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAT  >  NC_000913_3_vch_tpiA/263971‑264238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑