Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	4,604,746	C→A	A186E (GCA→GAA)	bglJ →	bgl operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	4,604,746	0	C	A	100.0%	124.6 / NA	37	A186E (GCA→GAA)	bglJ	bgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/19);  total (18/19)

GACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTACT  >  NC_000913_3_vch_tpiA/4604600‑4604889                                                                                                                                                   |                                                                                                                                                gaccgaccgacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGaac                                                                                                                                               <  1:428108/149‑1 (MQ=255)     gaccgacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATaa                                                                                                                                           >  2:332274/1‑149 (MQ=255)              aaCAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTTCGCTTTTTGTCGCGTGGCTACTCAATGGCACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAAAAATTTAAtgt                                                                                                                                  >  1:82850/1‑149 (MQ=255)               aCAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAtgtg                                                                                                                                 >  1:97425/1‑149 (MQ=255)                  aTCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGGGAGAAATATTGCATTAAACATTGTTTTGCTACCTTTGGACACGAACTGCCGACCTGCTGAAACGAAATATCAAAACGTTCCGTGAACAGAAAATTAATGTGATg                                                                                                                              >  1:491302/1‑149 (MQ=255)                            cATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAACCGATCCGTGAACATGAATTTAATGTGATGTCGAAACTgg                                                                                                                    <  2:297760/149‑1 (MQ=255)                            cATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTgg                                                                                                                    <  2:491302/149‑1 (MQ=255)                              ttAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGc                                                                                                                  <  1:328838/149‑1 (MQ=255)                                   cAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCaa                                                                                                             >  2:371446/1‑148 (MQ=255)                                    aaaGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGt                                                                                                            >  2:447491/1‑149 (MQ=255)                                         cGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGa                                                                                                       <  1:332274/149‑1 (MQ=255)                                             cGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa                                                                                                   <  2:238624/149‑1 (MQ=255)                                                tAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCATTTTTGACGCAggg                                                                                                <  2:311800/149‑1 (MQ=255)                                                     ccGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgtt                                                                                           <  2:97425/149‑1 (MQ=255)                                                        aCGGAGAGAGAAATATTGCGCTTTATGTCGCCAGGCGACCGAATTACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa                                                                                        <  1:466173/149‑1 (MQ=255)                                                                       ttGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTAGGCGTCAGTTCTGACGCAGGGTTATTGGAGGCCGCAGATATTCt                                                                         >  2:302544/1‑149 (MQ=255)                                                                         gcgaTTTAGGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCATCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGTGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGt                                                                       <  1:434353/149‑1 (MQ=255)                                                                          cgcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                                                                      >  1:304226/1‑149 (MQ=255)                                                                            cTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtat                                                                    <  1:494735/149‑1 (MQ=255)                                                                              ttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgt                                                                  <  2:244461/149‑1 (MQ=255)                                                                                  gTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGAGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGc                                                              >  1:431133/1‑149 (MQ=255)                                                                                   tCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCCGGGTTGTTGTAGGCCGCAGATATTCTGTTATGTATGCg                                                             >  1:140815/1‑149 (MQ=255)                                                                                        tGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACCATCCGTGAACATACATTTAATGTGATGTCGACACTGGGCATCAGTTCTGACGAAAGGTTGTTGGCAGCCGCAGATATACTGTTATCTATGGGGCAtt                                                        >  2:38915/1‑149 (MQ=255)                                                                                         ggCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTg                                                       >  2:450006/1‑149 (MQ=255)                                                                                                      cacaAATTCCCGAGCCGCTTAAACCCAATACCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAt                                          <  1:24342/149‑1 (MQ=255)                                                                                                        caAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtgt                                        <  1:450006/149‑1 (MQ=255)                                                                                                                        ttAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTaa                        >  1:85394/1‑149 (MQ=255)                                                                                                                        ttAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTaa                        >  1:382716/1‑149 (MQ=255)                                                                                                                         tAAACGCAATATCAAAACGATCCGTGAACATAAATTGAATGTGATGGCGTAACTGGGCGGCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTCTGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAAt                       <  1:226864/149‑1 (MQ=255)                                                                                                                           aaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAgg                                                                                                 >  1:134568/1‑73 (MQ=255)                                                                                                                           aaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAgg                                                                                                 <  2:134568/73‑1 (MQ=255)                                                                                                                             agtaaTATCAAAACGTTCCGGGAACTTAAATTTAATGTGATGTCGAAACTGGGCTTCAGTTCTGACGCAGGGTTGTTGGAGGCAGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAGGTGTTGCATCCCTATTAATCCGc                   <  2:11444/146‑1 (MQ=255)                                                                                                                               cAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTTTGTTAAGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat                 >  2:28674/1‑149 (MQ=255)                                                                                                                                        aaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCggg        >  1:186936/1‑149 (MQ=255)                                                                                                                                            aTCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa    <  1:447491/149‑1 (MQ=255)                                                                                                                                             tcggTGAACATAAAGTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAc   <  1:163712/146‑1 (MQ=255)                                                                                                                                             tCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATAATGCCCGTTTTAc   <  2:382716/149‑1 (MQ=255)                                                                                                                                              ccGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTACt  >  1:327228/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                GACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTACT  >  NC_000913_3_vch_tpiA/4604600‑4604889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑