Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	4,159,154	G→T	G212V (GGG→GTG)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	4,159,154	0	G	T	100.0%	118.3 / NA	37	G212V (GGG→GTG)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/19);  total (18/19)

TGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTT  >  NC_000913_3_vch_tpiA/4159019‑4159284                                                                                                                                        |                                                                                                                                   tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGAt                                                                                                                       <  1:139493/149‑1 (MQ=255)    ccatccatGACGATCACGCGTGGGCGAACCGCGAATGCGTGCCGATGTCCGATCTGGCAGGGGAAAAACTGCTGATGGTGGAAGATGGTCACTGTTTGCGCGACCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCAGATGAAGTTata                                                                                                                    <  2:149877/147‑3 (MQ=255)            gAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCcg                                                                                                            >  1:355304/1‑149 (MQ=255)              agagcccccGGGGGCGAACCGCGAATGCCTACCGATGGCCGATCGGGCAGGGGATAAACTGCTGATGCTGGAAGCTGCCCGCTGGTTGCGCGATCAGGCAATGGGTTTCTGTTTTGCAGCCGTGGCGGATGAAGATACACACTTCcgcg                                                                                                          <  1:334065/143‑1 (MQ=255)                 tCATCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCATGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGa                                                                                                       <  1:230978/149‑1 (MQ=255)                 tCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCCGATGCTGGAAGATGGTCACTGTTTGCGCGAGCAGGCAATGGGTTTCGGTTTTGTAGCCGTGGCGGATGAAGATACACACTTCCGCGCGa                                                                                                       <  1:511482/149‑1 (MQ=255)                     ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGa                                                                                                       >  1:155835/1‑145 (MQ=255)                     ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGa                                                                                                       <  2:155835/145‑1 (MQ=255)                     ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAg                                                                                                   >  2:534352/1‑149 (MQ=255)                     ccGTGGGCGAACCGAGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATGCACACTTCCGCGCGACCAg                                                                                                   >  1:324762/1‑149 (MQ=255)                         cgccGACCCGCGAAGTCTGACCGCTCGCCGATCTGGCAGGGGACAACCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCTTTGCGGATCAAGATACACACTTCCGCGCGACCAGCCTg                                                                                               <  1:184378/146‑1 (MQ=255)                             gAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATacaca                                                                                                                  <  1:220921/126‑1 (MQ=255)                             gAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATacaca                                                                                                                  >  2:220921/1‑126 (MQ=255)                             gAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaaa                                                                                           <  1:369541/149‑1 (MQ=255)                              aaCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCAGATGAAGATACACACTTCCGCGCGACCAGCATGGAAAc                                                                                          >  2:112453/1‑149 (MQ=255)                                ccGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActc                                                                                        <  1:549965/149‑1 (MQ=255)                                   cgAATGGGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                                                     <  2:278432/149‑1 (MQ=255)                                   cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                                                     <  1:243789/149‑1 (MQ=255)                                           tACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGGAACAtg                                                                             >  2:338292/1‑149 (MQ=255)                                             ccGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACCtggt                                                                           >  2:287913/1‑149 (MQ=255)                                              cGATGGCCGATCTGGAAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtg                                                                          <  2:7491/149‑1 (MQ=255)                                               gATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                                                                         <  2:394037/149‑1 (MQ=255)                                                attgCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCCCTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTTAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGAGCAGCCTGGAAACTCTGCGCAACATGGTggc                                                                        <  2:5135/146‑1 (MQ=255)                                                            gCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCgg                                                            <  1:461210/149‑1 (MQ=255)                                                              aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGa                                                          >  1:340983/1‑149 (MQ=255)                                                                    aaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACtt                                                    >  2:318126/1‑149 (MQ=255)                                                                    aaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTGTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCACCCTGGAAACTCTGCGCACCATGGTGCCAGCAGGTCGCGGCATCAcct                                                    >  2:34898/1‑147 (MQ=255)                                                                             gATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGCTTCTGTTTTGAAGCCGTGGCGGATGTAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCCACATGCTGGCGGCAGGTAGCGGGGTAACTTTACTGCCAg                                           >  2:65439/1‑149 (MQ=255)                                                                                            tCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                                                     <  1:35496/92‑1 (MQ=255)                                                                                            tCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                                                     >  2:35496/1‑92 (MQ=255)                                                                                             cACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgcc                           >  1:402708/1‑149 (MQ=255)                                                                                                tGTCTGCGCCCTCAGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACAGGCTTCGCCGCGACCAGCCTGGAAACTCCGCGCAACGTGGTGGCAGCAGCTACCGGGATCACTCTCCAGCCTCCGCTGGCTATGGCCCCGGa                        >  2:242263/1‑149 (MQ=255)                                                                                                            tggggaaTGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGACACACACGTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGa            <  1:468620/144‑1 (MQ=255)                                                                                                             aGGCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTATCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGACGGAGAGCAAACGCGAt           >  2:90229/1‑149 (MQ=255)                                                                                                              ggCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctggcagtaaa                              <  2:234000/129‑7 (MQ=255)                                                                                                              ggCAATGGGTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCagtaaa                              >  1:234000/1‑126 (MQ=255)                                                                                                                      gTTTCTGTTTTGAAGCCGTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgttgtt  >  1:460794/1‑149 (MQ=255)                                                                                                                                        |                                                                                                                                   TGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTT  >  NC_000913_3_vch_tpiA/4159019‑4159284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑