Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 111 [0] [1] 114 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)103 (0.960) 72/280 0.4 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 111 total aligned reads displayed.

GCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913/257761‑258055
                                                                                                                                                  |                                                                                                                                                    
gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAag                                                                                                                                                    <  1:540077‑M1/149‑3 (MQ=255)
  ttttgttaaAAATTTGCTAAAATTTGCCAATATGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGCCGTTACCGAGTGGACACCCGAAGAGCAGAATGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtg                                                                                                                                                  >  2:1327019‑M1/4‑145 (MQ=255)
   tgtgTTAAAACTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgc                                                                                                                                                 <  1:104976‑M1/149‑6 (MQ=255)
     tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaa                                                                                                                                               <  1:1360746‑M1/149‑8 (MQ=255)
     tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaa                                                                                                                                               <  1:378097‑M1/149‑8 (MQ=255)
     tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaa                                                                                                                                               <  1:796563‑M1/149‑8 (MQ=255)
      gtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAATCAGTTGCATCACAACAGGAGAAAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaa                                                                                                                                              <  1:1567218‑M1/149‑9 (MQ=255)
         aaaaaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGATGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagat                                                                                                                                           >  1:463892‑M1/1‑138 (MQ=255)
             aTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatc                                                                                                                                       <  2:648972‑M1/149‑16 (MQ=255)
             aTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatc                                                                                                                                       <  2:509280‑M1/149‑16 (MQ=255)
                 gCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgatt                                                                                                                                   <  1:778761‑M1/149‑20 (MQ=255)
                  cTAAATTTTTCCAATTTGGTAAAACTGTTGCATGACTACAGGTGATAGCTATGACTTTACCGAGTGGAAACGCGAAGATCATATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgtttc                                                                                                                                  <  1:1648860‑M1/149‑21 (MQ=255)
                  cTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACAGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattc                                                                                                                                  >  1:1261883‑M1/1‑129 (MQ=255)
                   tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACTCCCGAAGAGCAGATTGATCAAAAAATTTACCTCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                                                                 >  2:326008‑M1/1‑128 (MQ=255)
                   tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                                                                 >  1:1275485‑M1/1‑128 (MQ=255)
                    aaaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctt                                                                                                                                <  2:453724‑M1/149‑23 (MQ=255)
                       ttttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                             <  1:102915‑M1/149‑26 (MQ=255)
                          tGCCATTTTGGTAAAACAGTTTCATCACAACAGGAGATAGCAATCACCTTAACGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTTTATTCGTGAAGGTAagtgcaaagataatcgattctttttcga                                                                                                                          <  2:1473699‑M1/149‑29 (MQ=255)
                           gCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgat                                                                                                                         >  1:1246670‑M1/1‑120 (MQ=255)
                            ccAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttctatt                                                                                                                        >  2:1600912‑M1/1‑119 (MQ=255)
                             cAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatctattctttttcgattt                                                                                                                       >  1:1314680‑M1/1‑118 (MQ=255)
                              aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAAGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATTTTCGTGAAGGTAagtgcaaagataatcgattatttttcgatttt                                                                                                                      >  2:1559843‑M1/1‑117 (MQ=255)
                                  tGGTAATACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                  <  2:1626732‑M1/149‑37 (MQ=255)
                                  tGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                  <  1:265685‑M1/149‑37 (MQ=255)
                                    gTACAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGAGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATAATCGTGAAGGTAagtgcaaagatactcgattctttttcgattgtctggct                                                                                                                <  2:1634466‑M1/149‑39 (MQ=255)
                                    gTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggct                                                                                                                <  2:1656804‑M1/149‑39 (MQ=255)
                                      aaaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgt                                                                                                              >  2:682632‑M1/1‑109 (MQ=255)
                                       aaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgta                                                                                                             <  2:1336191‑M1/149‑42 (MQ=255)
                                         aCAGTTGCATCACAACAGGATATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagttaatcgattctttttcgattgtctggctgtatg                                                                                                           <  2:485514‑M1/149‑44 (MQ=255)
                                          cAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgc                                                                                                          <  1:239107‑M1/149‑45 (MQ=255)
                                            gTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtattcgt                                                                                                        >  2:673697‑M1/1‑103 (MQ=255)
                                               gCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggccgtatgcgtcaa                                                                                                     <  1:652673‑M1/149‑50 (MQ=255)
                                                cATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                                    >  1:453498‑M1/1‑99 (MQ=255)
                                                cATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                                    >  1:1064158‑M1/1‑99 (MQ=255)
                                                  tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                                                                  <  1:825597‑M1/149‑53 (MQ=255)
                                                  tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                                                                  >  1:1152294‑M1/1‑97 (MQ=255)
                                                    acaacaGGAGATAGCAAGGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACAGCACTAGGCCCGTATATTCGGGAAGGTAagtgcaaagataatcgattatttttcgattgtctggctgtatgcgtcaaagtga                                                                                                >  2:860794‑M1/1‑95 (MQ=255)
                                                      aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                            >  2:1608661‑M1/1‑93 (MQ=255)
                                                      aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                            <  1:1608661‑M1/119‑27 (MQ=255)
                                                        caGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATAAAAAAATTTACCGCACTAGGCCCGGATATTCGTGAAGGTAagtgcaatgataatctattctttttcgattgtgtggctgtatgcgtcaacgtgaagcc                                                                                            >  1:997640‑M1/1‑91 (MQ=255)
                                                                  cAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggta                                                                                  >  1:425343‑M1/1‑81 (MQ=255)
                                                                  cAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAactgcaaagataatcgattctatttcgattgtctggctgtatgcgtcaacgtgaatccggcaccggaa                                                                                  >  1:375328‑M1/1‑81 (MQ=255)
                                                                    aTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttctctagtctggctttatgcgtcaacgtgaaaccggcaccggaagt                                                                                >  2:490973‑M1/1‑79 (MQ=255)
                                                                    aTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagt                                                                                <  2:967464‑M1/149‑71 (MQ=255)
                                                                      gACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgc                                                                              <  2:815889‑M1/149‑73 (MQ=255)
                                                                        cGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                            <  2:437347‑M1/149‑75 (MQ=255)
                                                                        cGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                            <  1:632530‑M1/149‑75 (MQ=255)
                                                                        cGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                            <  1:255713‑M1/149‑75 (MQ=255)
                                                                        cGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCACGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                            <  1:382958‑M1/149‑75 (MQ=255)
                                                                        acttacCTAGTGGACACCCTAAGAGCAGATTTATAAAAAAATGTAACGCACTAGGCACTGATATTCGTGAAGGTAagtgcaaagataagcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                            <  1:1202078‑M1/147‑75 (MQ=255)
                                                                         gTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtg                                                                           >  2:909800‑M1/1‑74 (MQ=255)
                                                                         gTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaaagtgaaaccggcaccggaagtgcgtg                                                                           >  2:1244474‑M1/1‑74 (MQ=255)
                                                                         gTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttatcgattgtcttgctgtttgcgtcaacgtgaaaccggcaccggaagtgcggg                                                                           >  2:1717355‑M1/1‑74 (MQ=255)
                                                                               gAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattct                                                                     <  1:1244474‑M1/149‑82 (MQ=255)
                                                                                tgtGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGCTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                                    <  1:218159‑M1/148‑83 (MQ=255)
                                                                                aGTGTACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                                    <  1:1588941‑M1/149‑83 (MQ=255)
                                                                                aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATAATCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                                    <  2:226174‑M1/149‑83 (MQ=255)
                                                                                 gttgacaCACGATGAGCAGATTGTTCAAAAAATTTTCCGCAGGTGGCCCGTATATTCGGGAAGGTAagtgcaacgatattcgagtctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                                   <  1:1206678‑M1/146‑84 (MQ=255)
                                                                                 gTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                                   <  1:648564‑M1/149‑84 (MQ=255)
                                                                                  tGGACACACGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggg                                                                  >  1:425047‑M1/1‑65 (MQ=255)
                                                                                    gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggc                                                                >  2:911521‑M1/1‑63 (MQ=255)
                                                                                       acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcacctgaagtgcgtgaattctggggctgg                                                             >  1:1031856‑M1/1‑60 (MQ=255)
                                                                                           gAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                         >  1:263097‑M1/1‑56 (MQ=255)
                                                                                            aaGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggat                                                        <  2:724766‑M1/149‑95 (MQ=255)
                                                                                             atagcaGATTGATCAAAAAATTTAACGCAATAGGCCCGTATATTCGGGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtctgcgtcaacgtgtaaccggcaccggaagtgcgtgaattctggggctggtggatg                                                       <  1:1350877‑M1/147‑96 (MQ=255)
                                                                                                  aGATTGATCAAAAAATTAACCGCACTAGGCCCGTATATTCGTGAAGGAAagggcaaagataatcgattctttttcgattgtctggctgtatgcgtctacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagct                                                  <  2:304940‑M1/149‑101 (MQ=255)
                                                                                                    aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                                <  2:12596‑M1/149‑103 (MQ=255)
                                                                                                    aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatagtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcttgaattatggggctggtggatggagctgg                                                >  2:1743703‑M1/1‑47 (MQ=255)
                                                                                                         tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                           >  2:1059482‑M1/1‑42 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         >  1:1315286‑M1/1‑40 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         >  1:1838312‑M1/1‑40 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         >  2:1531582‑M1/1‑40 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         >  1:416971‑M1/1‑40 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         >  2:1328713‑M1/1‑40 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaaagtgaaaccggcaccggaagtgcgtgaattctggggcaggtggatggagctcgaagcgca                                         >  1:1406441‑M1/1‑40 (MQ=255)
                                                                                                            aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacccgcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                        >  1:974679‑M1/1‑39 (MQ=255)
                                                                                                              aaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagagcaaagataatcgattctttttcgattgtctggcggtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcagga                                      <  1:860794‑M1/149‑113 (MQ=255)
                                                                                                              aaaGTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatctattctatttcgagtgtctggcggtatgcgtcaacgtgaaaccggcaccggaagggcgtgaagtctggggcgggtggatggagctggaagcgcagga                                      >  1:1625350‑M1/1‑37 (MQ=255)
                                                                                                                   tACCGCACTACGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattggcgggctgtatgcgtcaacgtgaaaccggcaccggaagggcgttaattctggggctggtggatggagctggaagcgcaggaatccc                                 <  1:585790‑M1/149‑118 (MQ=255)
                                                                                                                      cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgaatcgttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattatggggcaggtggatggagctggaaacgcaggaataccgtt                              >  2:1232618‑M1/1‑29 (MQ=255)
                                                                                                                        cACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttt                            >  2:1406619‑M1/1‑27 (MQ=255)
                                                                                                                             cccccgtATAATCGTGACGGTAagtgcaaagataatcgatgccttttcgaatgtctggctgtatgcgtcaacgtgaaaccggcaccggaagggcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttaccta                       <  2:1086095‑M1/147‑128 (MQ=255)
                                                                                                                               cccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatggtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctaca                     >  1:339491‑M1/1‑20 (MQ=255)
                                                                                                                                ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                    >  2:1526503‑M1/1‑19 (MQ=255)
                                                                                                                                ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggcagtatgcgtcaacgtgaacccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                    >  1:591995‑M1/1‑19 (MQ=255)
                                                                                                                                    atatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttac                >  1:281797‑M1/1‑15 (MQ=255)
                                                                                                                                    ataaTCGTGAATGTAagtgcaaagataatcgtgtctttttcgattgtcgggctggatgcggcaacgggaaaccggcaccggaagtgcgtgaatgctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttac                <  2:1321408‑M1/149‑135 (MQ=255)
                                                                                                                                      atTCGTGAAGGTAagtgcaaagattatcgattctttttcgattgtctggctgtatgcggcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacca              <  1:1559843‑M1/149‑137 (MQ=255)
                                                                                                                                      atTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacca              >  1:1286154‑M1/1‑13 (MQ=255)
                                                                                                                                      atTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcggcaacgttaaacagtcaccggaattgagtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacca              <  1:1732893‑M1/149‑137 (MQ=255)
                                                                                                                                          tagaaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtattcgtcaacgtgttaccggcactggaagtgcgtgaattctggtgctggtggatggagctggaagcgctggaatcccgttttacctacagttaccagttt          <  1:219615‑M1/147‑141 (MQ=255)
                                                                                                                                          gTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggactcccgatttacctacagttaccagttt          >  2:1241286‑M1/1‑9 (MQ=255)
                                                                                                                                          gTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagttt          >  2:351244‑M1/1‑9 (MQ=255)
                                                                                                                                           tGAAGGTAagtgcatagattatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg         <  1:1717355‑M1/149‑142 (MQ=255)
                                                                                                                                           tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg         >  2:1735056‑M1/1‑8 (MQ=255)
                                                                                                                                            gAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggtgctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttgg        >  1:115437‑M1/1‑7 (MQ=255)
                                                                                                                                             aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt       >  1:24755‑M1/1‑6 (MQ=255)
                                                                                                                                                gTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctg    <  1:1406619‑M1/149‑147 (MQ=255)
                                                                                                                                                  aagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  <  2:1166740‑M1/149‑149 (MQ=255)
                                                                                                                                                  aagtgcaaacataatcgattctttttcgcttggctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  <  2:463892‑M1/149‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913/257761‑258055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: