breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
MC JC 1,397,381 Δ13,756 bp [ynaJ][ttcA] 15 genes
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,088,193 C→A intergenic (+333/‑91) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,183,214 C→T T657I (ACC→ATC)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 366213 366434 222 25 [21] [21] 22 [lacZ]–[lacI] [lacZ],[lacI]
* * ÷ NC_000913 3423749–3424234 3424562–3424238 5–814 22 [21] [20] 23 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4110730 4111487 758 23 [20] [20] 22 [tpiA] [tpiA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1158504 =56 (0.970)18 (0.350)
+CAGATTGGTGAATA
17/250 2.8 29.0% coding (636/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component
?NC_000913 = 1158538 42 (0.730)coding (670/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component
* ? NC_000913 = 12994980 (0.000)49 (0.880) 42/270 0.4 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein