Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_pgi	3,316,730	A→C	L266R (CTG→CGG)	nusA ←	transcription termination/antitermination L factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_pgi	3,316,730	0	A	C	100.0%	68.0 / NA	22	L266R (CTG→CGG)	nusA	transcription termination/antitermination L factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/14);  total (8/14)

GTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCT  >  NC_000913_3_vch_pgi/3316588‑3316873                                                                                                                                               |                                                                                                                                                gTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCCGTTCAg                                                                                                                                           >  1:365215/1‑149 (MQ=255)          ttCAACGGCCATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACTAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCg                                                                                                                                  <  1:345257/149‑1 (MQ=255)              aCGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGAAGGTATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTg                                                                                                                              >  1:108758/1‑149 (MQ=255)                                                      tAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGa                                                                                      <  2:168329/149‑1 (MQ=255)                                                        ctaGCAACGTTTGCCGAGGCCATTGCGTTAATCACGAACTGAGCCGGGTGATAATCCCACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACATACCGGATc                                                                                    <  2:82072/147‑1 (MQ=255)                                                         aaGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGaa                                                                                                                            <  1:159438/108‑1 (MQ=255)                                                         aaGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGaa                                                                                                                            >  2:159438/1‑108 (MQ=255)                                                               cGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGt                                                                             <  2:228172/149‑1 (MQ=255)                                                                     ccGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCg                                                                       <  2:134073/149‑1 (MQ=255)                                                                                  gTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGAGAATATCTATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACcg                                                          <  1:366039/149‑1 (MQ=255)                                                                                           gAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCg                                                 <  2:317888/149‑1 (MQ=255)                                                                                                   ccGGGTTATCATCCCACAGGACGATATCGTTACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGaa                                         <  1:128814/149‑1 (MQ=255)                                                                                                             atcCCACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGCGGACACGGATcgc                               >  2:15806/1‑149 (MQ=255)                                                                                                               cccACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGa                             <  2:8852/149‑1 (MQ=255)                                                                                                                ccACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTGGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGCTTTTCTCACGAGAACCCGGCTCGCGAg                            >  2:100820/1‑149 (MQ=255)                                                                                                                ccACAGGACGATATCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTTAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAg                            <  2:188663/149‑1 (MQ=255)                                                                                                                 cacaGGACGATATCGATACGCTCGCCACCACGTGCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTATCGGATCGATACGTTTATCGTTGCTTTTCACCGCGATTTTCGCACCCGAACCCGGATCGCGAGc                           >  2:355392/1‑149 (MQ=255)                                                                                                                            atCGATACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTaa                <  1:30909/149‑1 (MQ=255)                                                                                                                                aTACGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTc            >  2:249731/1‑149 (MQ=255)                                                                                                                                   cGCTCGCCACCCCGTTCCGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAAt         <  1:197378/149‑1 (MQ=255)                                                                                                                                   cGCTCGCCACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATTCCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAAt         <  1:196472/149‑1 (MQ=255)                                                                                                                                          cACCCCGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACGGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTAAATCActtct  >  1:358355/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                GTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCT  >  NC_000913_3_vch_pgi/3316588‑3316873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑