Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_pgi526,7960CG44.4% 20.2 / 34.0 37A1179G (GCG→GGG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (5/15);  new base G (11/5);  total (17/20)
Fisher's exact test for biased strand distribution p-value = 1.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAC  >  NC_000913_3_vch_pgi/526649‑526908
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ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                 <  2:125977/149‑1 (MQ=255)
ttGCGCAGTGCGGGCGGAGGGTGGAGACACGGGCCGCACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTTCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                 <  2:341817/149‑1 (MQ=17)
  gcgcAGTGCGGGCTGACGGTGGAGCAGCTGCGCACACAGGTCGCGCCGGAATACACACCGGCGCGAAAAGCTCATCTTCATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGa                                                                                                               <  2:180829/149‑1 (MQ=11)
           gggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgataa                                                                                                      >  2:990/1‑147 (MQ=255)
               tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                  <  2:137982/149‑1 (MQ=255)
               tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCCAAGCCGGCAATACGACGTGGGGCGGGGAGTCCGATAAATgg                                                                                                  >  2:150493/1‑149 (MQ=11)
               tGACGGTGGACCAACGGGCCAGACAGGTGGAGCCGGAATGCACACCGGCGCGAACAGCTCGTCTTTATCGCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAACACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                  <  1:338426/149‑1 (MQ=17)
                gACGGTGGAGCAACTGGCCGGACAGGTGGGGCCGGAATACACATCGGCGCGAAAAGCTCATATTTATCACTGCGACCCCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGCGGAATATGATGAATggg                                                                                                 <  2:345661/149‑1 (MQ=14)
                    gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCaa                                                                                             >  1:343195/1‑149 (MQ=255)
                        aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAg                                                                                         <  2:347984/149‑1 (MQ=255)
                        aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCGTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAg                                                                                         <  1:208871/149‑1 (MQ=255)
                         gCAACGGGCCAGACAGGTGGAGCCGGAATACACCCCGGCGCGAAAAGCTCCTCTATATCACTGCGACCAGCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGc                                                                                        <  2:389337/149‑1 (MQ=17)
                            aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATACGCCAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCtga                                                                                     >  1:90691/1‑147 (MQ=11)
                              tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                   >  1:87150/1‑149 (MQ=255)
                                gcacaaCAGGTGGAGCCGGAATCCACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCAATACGGCGTGGCGGGGAGAGAGTCATGAAGGGGGCAACCAGCTTAATga                                                                                 >  2:374887/6‑149 (MQ=11)
                                gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                 <  1:990/149‑1 (MQ=255)
                                   aggcAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATTTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                              <  2:381914/146‑1 (MQ=255)
                                       cggTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCGATACGGCTTGGCGCCGGTATTTTTATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                          <  1:290850/148‑1 (MQ=11)
                                        ggTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATAGGGCAACAAGCTTAATGAGGAGAAcc                                                                         >  2:54786/1‑149 (MQ=11)
                                         gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCACCGAAGACGGCAATACGGCGTGGCGGGGGGAGTCGGAAGAATGGGGCAACCAGCCTAATGTGCAGAAccc                                                                        >  2:156022/1‑149 (MQ=11)
                                          tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCg                                                                       >  1:362294/1‑149 (MQ=255)
                                           ggAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGTGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCAATACAGCCTCGCGCGTGGTAAATTCTGAATAGGACAACCAGCTTCGTTAGGAGAAccctc                                                                      >  2:45656/1‑147 (MQ=14)
                                              gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGGACCCGcatc                                                                   >  1:312935/1‑149 (MQ=11)
                                              gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGAGGACTGCCGCTGGCGCTTATCAGCGAAGACGCCAATACGGCGTGGCGCGGGGAGAATGATGAATGGCGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                   >  1:159601/1‑149 (MQ=11)
                                                  gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                              <  2:330173/134‑1 (MQ=255)
                                                  gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                              >  1:330173/1‑134 (MQ=255)
                                                  gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCGTGTCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                               >  1:308247/1‑146 (MQ=11)
                                                        acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                         >  1:140545/1‑149 (MQ=255)
                                                              gcgcGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCTATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGGGGACCCGCATCATGTGTATCAGCCGTa                                                   >  1:74763/1‑149 (MQ=255)
                                                               cgcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAc                                                  <  1:375834/149‑1 (MQ=255)
                                                                         tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                        <  1:320609/149‑1 (MQ=255)
                                                                          cATCTTTGTCACTGCGACCCCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCTGCATCATGTGTATCAGCCGTACCGTCTGCCAgg                                       <  1:36413/149‑1 (MQ=255)
                                                                                       gcgcCCACCGGTGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGACAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                          <  1:358659/149‑1 (MQ=255)
                                                                                                 gggACTGCCGCTGGGGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAg                <  1:305948/149‑1 (MQ=255)
                                                                                                       gccgccCGCCCTAATCCGCGAAGACGGCCAGACGGCCTGGGGCCCGTAATAAAATGAATTGCGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGt          <  2:336621/149‑1 (MQ=14)
                                                                                                        ccGCTGGCGCTTATCAGCGAAGACGACAATACGGCGTGGAGCGCGGGATATGATGAATGGGGCAACCGGCTTAATGAGGAGAACACGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGGAGCATGATGAGGAATAAGCGCTGTa         >  1:354111/1‑149 (MQ=17)
                                                                                                         cGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTAc        <  1:63822/149‑1 (MQ=255)
                                                                                                             ggCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACtata    <  1:198504/149‑1 (MQ=255)
                                                                                                               cgcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAc  <  1:213663/149‑1 (MQ=255)
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TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAC  >  NC_000913_3_vch_pgi/526649‑526908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑