New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi 724063 =23 (1.040)3 (0.140) 3/268 2.9 12.6% coding (352/2685 nt) kdpD fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein
?NC_000913_3_vch_pgi 724085 = 20 (0.950)coding (330/2685 nt) kdpD fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein
Rejected: Frequency below/above cutoff threshold.

ACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTCCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTAACGGCAGAACAGCCAGCCCTTCC  >  NC_000913_3_vch_pgi/723918‑724199
                                                                                                                                                 |                                                                                                                                         
aCGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTCCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGCTACCCCGCCAGCGTCTGGAATGCCGGGACCCTgg                                                                                                                                       >  1:14079/1‑149 (MQ=255)
 cGGCTTCCCCTACCTGAATGCCGGTGACGCCGCTGACCACATCATTCAGACTTTCCAGATGTTGAACGTTGACGGTAGTGAAAACAGCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGgc                                                                                                                                      <  1:374797/149‑1 (MQ=255)
    tttCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTCCAGATGCTGAACGTTGACGGTAGTGTACACATCACTGCCAGCTTCCAGCACTTCTTCGCTATCGTACCAGCGTTTGGGATCACGGTAACCTGCCGCa                                                                                                                                   >  2:273161/1‑149 (MQ=255)
       cccGTACCTTAATTCCGGTGACGCCGCTGACCACATCATTCTGACTTTCCAGATGCTGAACGTTGATGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTa                                                                                                                                <  1:165448/149‑1 (MQ=255)
                   ttCCGGTGACGACGCTGACCACATCATTCAGACTTTCCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGCACGTGGAGCTTCACGGTGCGCCAGt                                                                                                                    >  1:247403/1‑149 (MQ=255)
                               gaagggctggctgttctgccgttaaaacgccaggcgtaccgtgggcggcatatcagcgagtttgatctcgatgccgccctcgcccgccgcccggcgctgatcttaatggacgaaCCTGGCGCATTACTGTGCGCCAGTTCGTCCATTaa                                                                                                         >  2:300323‑M1/115‑149 (MQ=255)
                                        catcatTCAGACTTTCCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGcc                                                                                               >  1:260843/1‑149 (MQ=255)
                                            atTCAGACTTTCCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTa                                                                                                          <  2:66857/134‑1 (MQ=255)
                                            atTCAGACTTTCCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTa                                                                                                          >  1:66857/1‑134 (MQ=255)
                                               cAGACTTTCCAGATGCCGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGggcggc                                                                                        <  2:301727/149‑1 (MQ=255)
                                                        cAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAggg                                                                               >  1:214398/1‑149 (MQ=255)
                                                               tGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATc                                                                        >  1:90368/1‑149 (MQ=255)
                                                                    gTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGcggcgg                                                                                      <  1:352361/130‑1 (MQ=255)
                                                                    gTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGcggcgg                                                                                      >  2:352361/1‑130 (MQ=255)
                                                                             aGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGc                                                          <  1:270304/149‑1 (MQ=255)
                                                                                      gcgagtttgatctcgatgccgccctcgcccgccgcccggcgctgatcttaatggacgaaCTGGCGCATTACTGTGCGCCAGTTc                                                                                                                  >  2:113406‑M1/60‑84 (MQ=255)
                                                                                      gcgagtttgatctcgatgccgccctcgcccgccgcccggcgctgatcttaatggacgaaCTGGCGCATTACTGTGCGCCAGTTc                                                                                                                  <  1:113406‑M1/25‑1 (MQ=255)
                                                                                          aTGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGccc                                             >  1:240905/1‑149 (MQ=255)
                                                                                                           ttcttcGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGtt                            <  1:333597/149‑1 (MQ=255)
                                                                                                              ttcGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTa                         >  1:38014/1‑149 (MQ=255)
                                                                                                                  atatCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTAACgg                     <  1:274772/149‑1 (MQ=255)
                                                                                                                    accccgCCAGCTGTTGGGATGCAGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTCAGAGCAGCCCCGCGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTAACGGCa                   <  2:303516/144‑1 (MQ=255)
                                                                                                                       cTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGggc                                                                                           >  1:326625/1‑74 (MQ=255)
                                                                                                                       cTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGggc                                                                                           <  2:326625/74‑1 (MQ=255)
                                                                                                                            aGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATGTGCCGCCCACGGTACGCCTGGCGTTTTAGCGGCAGCAcagcc           >  1:381258/1‑149 (MQ=255)
                                                                                                                                     gATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTAACGGCAGAACAGCCAGCCCTTcc  <  2:214398/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                         
ACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTCCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCTGGCGTTTTAACGGCAGAACAGCCAGCCCTTCC  >  NC_000913_3_vch_pgi/723918‑724199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.