Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_pgi	4,188,953	G→A	E1202K (GAA→AAA)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_pgi	4,188,953	0	G	A	100.0%	52.1 / NA	18	E1202K (GAA→AAA)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

GGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACG  >  NC_000913_3_vch_pgi/4188808‑4189097                                                                                                                                                  |                                                                                                                                                 ggCAATACGTGCTGGAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAAc                                                                                                                                               <  2:305555/149‑1 (MQ=255)            cTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACgt                                                                                                                                    <  2:7017/149‑1 (MQ=255)                                  cTTCGGTACAGAAACCAAAGGTAAACCTCGTCTGGTTATCAACCCGGTAGACGGTAGCGATACGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAAGTAAACGTGTCGACAGAGGGGACGGAATTTCCGACGGTc                                                                                                              >  2:65978/1‑149 (MQ=255)                                            gAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGcgcc                                                                                                    >  1:65681/1‑149 (MQ=255)                                                          aCGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATtctgc                                                                                      >  2:200035/1‑149 (MQ=255)                                                                    gTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGACATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCgtgg                                                                            >  2:118093/1‑149 (MQ=255)                                                                                         aGCAATCCGTACGAAGAGATGATTCCGAAATGGCGTCAACTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTa                                                       <  2:205114/149‑1 (MQ=255)                                                                                                 gTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGAGCCGCGTGACCGTCTGCGTCTGCGTGGTGTTCATGCTGTTACGCGTTACATTGTTa                                               >  1:205377/1‑149 (MQ=255)                                                                                                  tACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTaa                                              >  1:185671/1‑149 (MQ=255)                                                                                                   aCGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACAGGAGTGAAGCGCCGCACGACATTCTGCGTCTGCTTGGTGTTCATGCTGTTACTCGTTACATCGTTAAc                                             <  1:304400/149‑1 (MQ=255)                                                                                                     gAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTACGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGa                                           >  1:4992/1‑149 (MQ=255)                                                                                                        gagaTGATTCCGAAATCGCGTCAGCTCAACGTGTTCGAAGCTAAACGTGTAGAACGTGGTTACGTAAGTTCCGACGGTCCTGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGt                                        <  1:65978/149‑1 (MQ=255)                                                                                                                  cGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACAAGGTGGACTACGTACCGACGGAACAGAAGCGCCGCACGCCATTCTGCGTCTGAATGCTGTTCATCATGTTACTCGTCACCTCGTTAACTGACTACacgacggg                              >  2:104927/1‑147 (MQ=255)                                                                                                                        ggggttggCTCAACGGGTTGGAAGGTAATCGTGTAGAACGTGGGGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAAAGAAGTACAGGACGTATACCGt                        <  2:285084/142‑1 (MQ=255)                                                                                                                        ggCGTCAGGTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGt                        <  2:252717/149‑1 (MQ=255)                                                                                                                                            gAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTaa    <  1:118093/149‑1 (MQ=255)                                                                                                                                            gAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTaa    <  2:65681/149‑1 (MQ=255)                                                                                                                                              aGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACg  >  1:184469/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                 GGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACG  >  NC_000913_3_vch_pgi/4188808‑4189097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑