Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_pgi 1,904,693 G→T G267C (GGC→TGC)  manZ → mannose‑specific enzyme IID component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_pgi1,904,6930GT92.6% 67.0 / ‑0.4 27G267C (GGC→TGC) manZmannose‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (16/9);  total (17/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01

CATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTTTATCTGGAGGATTAATGACAATCACGGA  >  NC_000913_3_vch_pgi/1904548‑1904814
                                                                                                                                                 |                                                                                                                         
cATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGct                                                                                                                        >  1:34791/1‑149 (MQ=255)
     cTGACCAGACGGTCAAAGAACGCGTTACTACTGTATTGGCTATTGTGGTAATATTTCCCTCAAGCCTAGTAAAAATGGTGCAAACGGTGGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCttcttc                                                                                                                   <  2:394978/149‑1 (MQ=255)
     cTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCttcttc                                                                                                                   >  1:520025/1‑149 (MQ=255)
       gACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGt                                                                                                                 <  1:167924/149‑1 (MQ=255)
           aGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATc                                                                                                             >  1:185626/1‑149 (MQ=255)
                      gAACACGTTACTACTGACCAGACTATTCTGGAACAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTCCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCGGTGGATCATCGTTTGCTTCCTCGCCCTCGATATCGCtcg                                                                                                  >  2:103700/1‑147 (MQ=255)
                                  actGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCgg                                                                                      <  2:185626/149‑1 (MQ=255)
                                   ctGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGc                                                                                     >  1:311913/1‑149 (MQ=255)
                                     gTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGc                                                                                        >  1:294390/1‑144 (MQ=255)
                                     gTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGc                                                                                        <  2:294390/144‑1 (MQ=255)
                                         aGACTATTCTGGACCAGTTAATGCCAGGCCTGGTCCCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGATTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCctgctg                                                                               >  1:238055/1‑149 (MQ=255)
                                         aGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTGTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCctgctg                                                                               >  1:103670/1‑149 (MQ=255)
                                                  tGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTaa                                                                      >  1:423589/1‑149 (MQ=255)
                                                      ccAGTTAATGCCAGGCCTGGTACCACTGCTGCTTACCTTTGCTTGTATGTGGCTACTTCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACt                                                                  <  1:88550/149‑1 (MQ=255)
                                                         gTTAATGCCAGGCCTGGTACCACCGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACtgta                                                               <  1:83800/149‑2 (MQ=255)
                                                                 cAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTa                                                       >  1:72443/1‑149 (MQ=255)
                                                                                tgctgctgACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAGGACTGTTGTACACTACCGGGGCCTTTTGGc                                        >  1:27644/1‑149 (MQ=255)
                                                                                 gctgctgACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGcc                                       <  2:311913/149‑1 (MQ=255)
                                                                                         ccTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGttttt                               >  2:310435/1‑149 (MQ=255)
                                                                                          cTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTAAGCTACCGGGGCCTTTTGGCCCCGttattt                              >  2:17677/1‑149 (MQ=255)
                                                                                              gCTTGTATGTGGCTACTGCGTAAAAAAGTTAACCCGCTGCGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTTTATc                          <  2:377881/149‑1 (MQ=255)
                                                                                                 tGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTCAACTACCGGGGCCTTTTGGCCCCGTTTTTTTATTTgg                       >  2:1861/1‑149 (MQ=255)
                                                                                                   tATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACCCTATCGGGGCCTTTTGGCCCCGTTTTTTTATCTggag                     <  1:328661/149‑1 (MQ=255)
                                                                                                         gCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGTGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGGTTTTTTATCTGGAGGAttca               >  2:7356/1‑147 (MQ=255)
                                                                                                           tACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTTTATCTGGAGGATTAATg             >  1:434802/1‑149 (MQ=255)
                                                                                                           tACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTGTTTTATCTGGAGGATTAATg             >  1:382067/1‑149 (MQ=255)
                                                                                                                      aaaGTTAACCCGCTGTGGATCATCGTTTGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTTTATCTGGAGGATTAATGACAATCACGGa  <  1:47845/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                         
CATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTTTATCTGGAGGATTAATGACAATCACGGA  >  NC_000913_3_vch_pgi/1904548‑1904814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑