Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_pgi 4,343,923 G→A L184L (CTG→CTA)  melB → melibiose:sodium symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_pgi4,343,9230GA96.7% 92.7 / NA 30L184L (CTG→CTA) melBmelibiose:sodium symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/16);  minor base C (1/0);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGA  >  NC_000913_3_vch_pgi/4343778‑4344063
                                                                                                                                                 |                                                                                                                                             
tCGATAAACGTGAGCGCGAACAACTGGTTCCTTTTCCGCGTTATAGAATATGGCTTGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTa                                                                                                                                            <  1:404910/149‑1 (MQ=255)
    tAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGa                                                                                                                                        <  2:407732/149‑1 (MQ=255)
                    caacTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGt                                                                                                                        <  1:387186/149‑1 (MQ=255)
                      acTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGttt                                                                                                                      <  2:434801/149‑1 (MQ=255)
                       cTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAGTTATGTCGGCGGTGGCGCTCGGGGATTTGGCTTTCAGATGTTCACTCTCGTACTGATCGCCTTTTTTATTGTTTc                                                                                                                     >  1:29000/1‑149 (MQ=255)
                          gTTCCTTCTCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGTAGGTGGCGATCGGGGCTTTGGCTTTTAGATGTTCCCTCTAGTACTGATCGCCTTTTTTATTGTTTCAAc                                                                                                                  <  2:189680/149‑1 (MQ=255)
                            tCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACca                                                                                                                <  1:328708/149‑1 (MQ=255)
                                  tCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCAtcatca                                                                                                          >  1:401097/1‑149 (MQ=255)
                                               gCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAAtgt                                                                                             <  1:507191/149‑1 (MQ=255)
                                                              tttGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTc                                                                              >  2:184989/1‑149 (MQ=255)
                                                                  ttACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCa                                                                         <  1:135333/149‑1 (MQ=255)
                                                                       gCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACaa                                                                    >  1:149476/1‑149 (MQ=255)
                                                                        cAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAAt                                                                   >  1:92165/1‑149 (MQ=255)
                                                                        cAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGCCACTACGGGATTTGGAACTCACCTGTTGACTCCAGTACTGGTCGCCTATTTTTTTTTTTCAGCCATGTTCAGTCTTTGCAAGCTTCATGAAGTCTTTTCG‑TCAGCCAAt                                                                   >  2:188361/1‑149 (MQ=255)
                                                                                  gCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACGCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTctg                                                         >  1:436235/1‑149 (MQ=255)
                                                                                    tACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTctgct                                                       <  1:329221/149‑1 (MQ=255)
                                                                                    tACCATTTGATAAGCATGGGTACGGTGGCGATCGTTGATTTGGCCTTCAGATGTTCACTCTAGTACTGAGCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTctgct                                                       >  2:467106/1‑149 (MQ=255)
                                                                                     aCCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTTTTTTTTTATCCATCATCACTCTGCGCAATGTGAATGAAGTCTTTTCG‑TCATACAATCAACCGTctactg                                                      >  2:514127/1‑149 (MQ=255)
                                                                                                    tGTCGGCGGTGGCGATTGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGa                                       <  2:405379/149‑1 (MQ=255)
                                                                                                       gggcggTGGGGATCGGGGATTTGGCTTTCAGATGTTCACTCCAGTACTGATCGTCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGacac                                    <  2:227971/148‑1 (MQ=255)
                                                                                                         gcggTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCTCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACtt                                  <  2:92165/149‑1 (MQ=255)
                                                                                                            gTGCCGATCGGGGATTTAGCTTTCAGATGTTCACTCTAGTTCTTCTCGACTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTaaa                               <  1:120923/149‑1 (MQ=255)
                                                                                                                 gATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCAt                          >  1:299950/1‑149 (MQ=255)
                                                                                                                         aTTTGGTGTTCAGGTGTTCACTCTAGTACTGATTGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTgcg                   <  2:376209/149‑1 (MQ=255)
                                                                                                                                  tCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCAt                                                                                         >  2:427839/1‑70 (MQ=255)
                                                                                                                                  tCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCAt                                                                                         <  1:427839/70‑1 (MQ=255)
                                                                                                                                      aTGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATaaaaa     <  1:112140/149‑1 (MQ=255)
                                                                                                                                         ttCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGa  >  1:514252/1‑149 (MQ=255)
                                                                                                                                         ttCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGa  >  2:465940/1‑149 (MQ=255)
                                                                                                                                         ttCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCACTAATTTATAAAAACGa  >  1:394276/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGA  >  NC_000913_3_vch_pgi/4343778‑4344063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑