Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_vch_pgi 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_vch_pgi 1299499–1300697 1300697 1–1199 31 [0] [0] 31 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi = 12994980 (0.000)31 (1.010) 26/280 0.3 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_vch_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTT  >  NC_000913_3_vch_pgi/1299353‑1299637
                                                                                                                                                 |                                                                                                                                           
cgcaaATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTGGCCACATCATAATCCTGGGCTTGCGGGAGAATAATTGAAATGATATTATTAATTCCACTGCCGTTGGTAGAGGAAAGTGCTAAata                                                                                                                                          >  2:7372‑M1/3‑146 (MQ=255)
 tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                         <  2:164892‑M1/149‑5 (MQ=255)
         cAGTTAGTTATAAGGATTTCCTTAACTGTTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGTCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                                                                                                                 <  2:380965‑M1/149‑13 (MQ=255)
          agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                <  1:308829‑M1/149‑14 (MQ=255)
            ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgt                                                                                                                              >  1:390415‑M1/1‑134 (MQ=255)
                    aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                                                                                                                      >  1:503411‑M1/1‑126 (MQ=255)
                     aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatttttaaattatt                                                                                                                     >  2:308852‑M1/1‑125 (MQ=255)
                           tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGACATGATATTATTAATTCCACTGCCTTTGATAGAGGAAAGTGCTAAataataatcaattgttcaattattgtgcat                                                                                                               >  2:310085‑M1/1‑119 (MQ=255)
                               tAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                           <  1:184571‑M1/149‑35 (MQ=255)
                                aaCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGACAGTGCTAAataataatcaatggttaaattattgtgcatttcac                                                                                                          <  2:480103‑M1/149‑36 (MQ=255)
                                   tGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATCTTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattattcttttcaatat                                                                                                       >  1:456674‑M1/1‑111 (MQ=255)
                                       tctcCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAATGCTAAataataatcaattgttaaattattgtgcatttcactactgga                                                                                                   <  1:488920‑M1/149‑43 (MQ=255)
                                                    atGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaa                                                                                      <  1:285441‑M1/149‑56 (MQ=255)
                                                         aTTTTCGCCATATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                 <  2:513927‑M1/149‑61 (MQ=255)
                                                             tCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagac                                                                             <  2:21488‑M1/149‑65 (MQ=255)
                                                                    atcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                     <  1:284906‑M1/102‑25 (MQ=255)
                                                                    atcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                     >  2:284906‑M1/1‑78 (MQ=255)
                                                                      catAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                    >  1:386995‑M1/1‑76 (MQ=255)
                                                                          aTCCTGGGATTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaagtgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaat                                                                <  2:144466‑M1/149‑78 (MQ=255)
                                                                                 gCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatt                                                         <  2:149738‑M1/149‑85 (MQ=255)
                                                                                          agaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaat                                                <  1:139656‑M1/149‑94 (MQ=255)
                                                                                              taatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttatccaatctctatttgattgaattgaa                                            <  1:136910‑M1/149‑98 (MQ=255)
                                                                                               acttgaAATGATATTATGAATTCCACTGGAGTTTGGAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                           <  2:107443‑M1/147‑99 (MQ=255)
                                                                                               aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                           <  1:236698‑M1/149‑99 (MQ=255)
                                                                                               aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                           <  1:195985‑M1/149‑99 (MQ=255)
                                                                                                                        cTGCCTTTGTGAAAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                  <  2:412921‑M1/149‑124 (MQ=255)
                                                                                                                        cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattcttaaattattgtgcatttcactactggaactgtaatcagtgaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtgaaggcatggatgcaa                  <  2:454243‑M1/149‑124 (MQ=255)
                                                                                                                            cTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatagaaagctggtatttaaggcatgcatgccagcga              >  1:541440‑M1/1‑22 (MQ=255)
                                                                                                                                      ggAAAGTTCTAAataataatcaattgttaaatgattgggcatttcactactggaactgtaatcagaaaagatagacatgcttagacaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga    <  2:461609‑M1/149‑138 (MQ=255)
                                                                                                                                        aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgata  <  1:485457‑M1/149‑140 (MQ=255)
                                                                                                                                        aaaGTGCTAAacaataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaagctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgata  <  1:208120‑M1/149‑140 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
CTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTT  >  NC_000913_3_vch_pgi/1299353‑1299637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: