New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi 1457261 =41 (1.300)4 (0.130) 4/274 4.1 9.6% coding (832/1071 nt) paaE ring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component
?NC_000913_3_vch_pgi 1457296 = 36 (1.200)coding (867/1071 nt) paaE ring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGTGGCGATGGAAACCAATTACAGTCTGGA  >  NC_000913_3_vch_pgi/1457122‑1457403
                                                                                                                                           |                                                                                                                                               
aCTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGCTGGGCGGGCTCGGGAAAGCGTGCTTAATGCcgacg                                                                                                                                        >  2:353156/1‑149 (MQ=255)
aCTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCcgacg                                                                                                                                        >  1:374155/1‑149 (MQ=255)
  ggggAAGGCCGGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTCGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGGCAGGATGGGCGGGATCGGGGAATCGCGCTTAATGCCCACGAt                                                                                                                                      >  2:285800/2‑149 (MQ=255)
    ggAATGCCAGATAAAACCCTTCATCTGGAGCGGTTTAATACGCGTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAATAGTGACTGTACGTCAGGATGGGCGGGTTCGGGAAATCGTGCTTCTTGCCGACGATGa                                                                                                                                    >  2:100886/1‑149 (MQ=255)
    ggAATGCCAGATAAAACCATTCAGCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGa                                                                                                                                    <  1:513813/149‑1 (MQ=255)
                           tCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTgc                                                                                                            >  2:539899/1‑149 (MQ=255)
                                agggggttAATACGCCGGGGACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCCCGATGCGGCATTGCGCCAg                                                                                                       <  2:379222/143‑1 (MQ=255)
                                    gTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAggggg                                                                                                   <  1:392635/149‑1 (MQ=255)
                                     tttAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGc                                                                                                  >  2:129555/1‑149 (MQ=255)
                                     tttAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTACAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGc                                                                                                  >  2:394634/1‑149 (MQ=255)
                                             gCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGGGGCATTAGCCCGAGGGGGGGATCTGc                                                                                          >  2:510184/1‑149 (MQ=255)
                                               cTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGCTGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGAACCGGCATTACGCCAGGGGGCGGACCTGccc                                                                                        >  2:268923/1‑149 (MQ=255)
                                               cTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGccc                                                                                        <  1:334668/149‑1 (MQ=255)
                                                 ggCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTa                                                                                      <  2:368428/149‑1 (MQ=255)
                                                           tCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCaaa                                                                            <  1:497304/149‑1 (MQ=255)
                                                           tCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCaaa                                                                            <  1:236202/149‑1 (MQ=255)
                                                           tCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCaaa                                                                            <  1:236200/149‑1 (MQ=255)
                                                               aCGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAggcg                                                                        <  1:467369/149‑1 (MQ=255)
                                                                 gTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAAGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGAAGAAAGCAGTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTCCCCTATGCCTGCAAAggcggc                                                                      >  1:494574/1‑149 (MQ=255)
                                                                       ttAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCtgt                                                                >  2:130520/1‑149 (MQ=255)
                                                                            gTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGCGCTGCCCCATGCCTGCAAAGGCGTCGGCTGTGCgcc                                                           >  2:315531/1‑147 (MQ=255)
                                                                                aaaGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATtc                                                                                                                            <  2:22643/81‑1 (MQ=255)
                                                                                aaaGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATtc                                                                                                                            >  1:22643/1‑81 (MQ=255)
                                                                                   ctgacctaCAAAAAGTTACTCTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCtgcaaa                                                    <  1:52706/142‑1 (MQ=255)
                                                                                         gACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGCACTGCCCTATGCCTGCAAAGGCAGCGTCTGTGCGACCtgcaaatgcaaa                                              >  1:381706/1‑149 (MQ=255)
                                                                                               aaGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGACTGCaaa                                                                            <  1:47502/113‑1 (MQ=255)
                                                                                               aaGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGACTGCaaa                                                                            >  2:47502/1‑113 (MQ=255)
                                                                                                aGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGtgctgc                                       <  1:62081/149‑1 (MQ=255)
                                                                                                      tGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCa                                 >  2:549642/1‑149 (MQ=255)
                                                                                                       gTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGAGCTGCAAATGCAAAGTGCTGCGTGGCaa                                >  1:332468/1‑149 (MQ=255)
                                                                                                          cGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGt                             <  1:394634/149‑1 (MQ=255)
                                                                                                            tCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGCCGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAGGGCGGCATCTGTGCGACCTGCAAATGCCAAGTGCCGCGTCGccccgtgg                           >  2:532110/1‑142 (MQ=255)
                                                                                                            tCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGTgg                           >  1:345857/1‑149 (MQ=255)
                                                                                                            tCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTAGGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGTgg                           <  1:130520/149‑1 (MQ=255)
                                                                                                              aGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGTGGCg                         >  1:253706/1‑149 (MQ=255)
                                                                                                                 aTGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGTGGCGATg                      <  1:456122/149‑1 (MQ=255)
                                                                                                                           tCGGGAAATCGGGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGTGGCGATGGAAACCAAtt            <  2:279201/149‑1 (MQ=255)
                                                                                                                            cGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGTGGCGATGGAAACCAATTa           <  2:345857/149‑1 (MQ=255)
                                                                                                                              ggAAATCGTGCTTAAAGCCGACGATGAAAGCATGACCGATGCGGCATTGCGCCAGGGGGAGGATCTGCCCTATGCCTGCAAAGGCGGGGTTTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGTGGCGATGGAAACCAATTACa         <  2:49138/149‑1 (MQ=255)
                                                                                                                                  aTCGTGCTTAATGCCGACGATGAAAGCATTCTCTACTCGGTAGTGTGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAATTGGCGATGGAAACCAATTACAGTCt     <  1:518058/149‑1 (MQ=255)
                                                                                                                                     gTGCTTAATGCCGACGATGAAAGCATTCTCGAAGCGGCATCGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAACGGCGCCGTCTGTGCGACCGGCAAATCCCAAGTGCTGCCGGGCAAGATGGCGATGGAAACAAGCTCCACTCTGGa  >  2:150435/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                               
ACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGTGGCGATGGAAACCAATTACAGTCTGGA  >  NC_000913_3_vch_pgi/1457122‑1457403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.