Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,354,297 G→T intergenic (‑247/‑428) yhcC ← / → gltB putative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,354,2970GT98.1% 174.1 / NA 53intergenic (‑247/‑428)yhcC/gltBputative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (28/24);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATAATCAATCAAAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTG  >  NC_000913_3_pae_pgi/3354151‑3354439
                                                                                                                                                  |                                                                                                                                              
gCATAATCAATCAAAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCt                                                                                                                                              <  1:884161/149‑1 (MQ=255)
 cATGATCAATCAAAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTg                                                                                                                                             <  1:490087/149‑1 (MQ=255)
  aTAATCAATCAAAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTgg                                                                                                                                            >  2:335479/1‑149 (MQ=255)
   caaccaatctaAATTACCGAAATTTCATGCATAATCACATAAATCACCTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGt                                                                                                                                           <  1:403193/145‑1 (MQ=255)
     atcaaacaAAATTACCTAAAATTGATGCATAATCCCATAAATCACTTGTGCTTATCTTGTGTCAGATTTTCTTATCTCCTGATAAAAAAAAAAAAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGttt                                                                                                                                         <  2:25529/149‑1 (MQ=255)
       caatcaaAATTACCGAAATTTCATTTATAATCACATAAATCACTTTGGCTTACCTTGTGTTAGATTTTTTTAACTTCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTg                                                                                                                                       <  2:621141/149‑1 (MQ=255)
       caatcaaAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTg                                                                                                                                       <  2:533482/149‑1 (MQ=255)
         atcaaAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTgtc                                                                                                                                     <  2:200948/149‑1 (MQ=255)
          tcaaAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGAAGTATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTgtcg                                                                                                                                    >  1:730380/1‑149 (MQ=255)
           caaAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTgtcgt                                                                                                                                   <  2:70652/149‑1 (MQ=255)
                ttACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGtt                                                                                                                              <  1:335479/149‑1 (MQ=255)
                   ccGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCCGttgca                                                                                                                           >  2:896458/1‑146 (MQ=255)
                    cGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTCAGGCAGTCTACAATTCTCGTTTTGTCGTCCCTTCAAg                                                                                                                          >  2:570466/1‑149 (MQ=255)
                     gAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAgg                                                                                                                         <  2:492636/149‑1 (MQ=255)
                      aaaTTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGc                                                                                                                        >  1:528407/1‑149 (MQ=255)
                         tttCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAgg                                                                                                                     >  1:843177/1‑149 (MQ=255)
                           tCAAGCATAATCACATAAATCGCTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGAt                                                                                                                   <  1:452233/149‑1 (MQ=255)
                               taaTAATCACATAAATTACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCGGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAgg                                                                                                               <  2:730380/147‑1 (MQ=255)
                                 aTAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGt                                                                                                             >  1:440774/1‑149 (MQ=255)
                                 aTAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGt                                                                                                             >  2:6495/1‑149 (MQ=255)
                                  tAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGtt                                                                                                            <  2:440774/149‑1 (MQ=255)
                                     tCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAac                                                                                                         >  1:598003/1‑149 (MQ=255)
                                       acaTAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAacac                                                                                                       >  1:530492/1‑149 (MQ=255)
                                         aTAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTTTTTTTTTTTTGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAACTTCTGGTTTTTTAATCAGTTCACGTCAGCCTCAGGGTTAacacac                                                                                                     >  2:218172/1‑149 (MQ=255)
                                               cccTTTTGCTTATCTTGTGTCACATTCTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTAt                                                                                               <  1:328579/147‑1 (MQ=255)
                                                   gtttcTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGTAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACa                                                                                           <  2:489022/145‑1 (MQ=255)
                                                               gtgtCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGa                                                                               <  2:530492/149‑1 (MQ=255)
                                                               gtgtCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGa                                                                               <  1:6495/149‑1 (MQ=255)
                                                                  tCAGATTTTTTTATTTCCTGATGGATTGTTGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTg                                                                            >  1:753213/1‑149 (MQ=255)
                                                                    aGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGtt                                                                          >  1:984914/1‑149 (MQ=255)
                                                                       tttttttATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTtct                                                                       <  1:1008354/149‑1 (MQ=255)
                                                                       tttttttATATCCTGGTGGATTTTAGGCAAAAACAGTAGCATGACACGTCATTACAAATTAGGGCAGTATAAAATTTTGGTTTTGTCGTCAGTTCAAGGGAGGATAAGGGTTAACAGACCTTTATGCCAGTCAGGAGTTGACTGttttt                                                                       >  2:996869/1‑147 (MQ=255)
                                                                        ttttttATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTtctt                                                                      <  1:218172/149‑2 (MQ=255)
                                                                           tttATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTACGGAATTGATTGTTTCTGTac                                                                   >  2:30650/1‑148 (MQ=255)
                                                                                     gATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCt                                                         <  2:843177/149‑1 (MQ=255)
                                                                                      aTGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACCCCTTTTTATCAGTCAGCAATTGACTGTTTCTCTAACGACTTCCCtt                                                        >  1:293196/1‑149 (MQ=255)
                                                                                      aTGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCtt                                                        >  1:877890/1‑149 (MQ=255)
                                                                                      aTGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCtt                                                        >  1:45925/1‑149 (MQ=255)
                                                                                       tGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCttt                                                       <  1:896458/149‑1 (MQ=255)
                                                                                            tttAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCa                                                                                       <  1:67704/112‑1 (MQ=255)
                                                                                            tttAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCa                                                                                       >  2:67704/1‑112 (MQ=255)
                                                                                                 gCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACATTTATAACAGTCAAGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTaac                                             >  2:868735/1‑148 (MQ=255)
                                                                                                       aCAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATaaa                                       >  2:448524/1‑149 (MQ=255)
                                                                                                           tAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATcc                                   >  2:779127/1‑149 (MQ=255)
                                                                                                               aTGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCAttt                               >  2:1018771/1‑149 (MQ=255)
                                                                                                                      gTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTc                        >  2:294945/1‑149 (MQ=255)
                                                                                                                          ttACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTGATGTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTc                    >  2:1039145/1‑149 (MQ=255)
                                                                                                                              cAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCAttt                >  1:259664/1‑149 (MQ=255)
                                                                                                                                 ttAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTtaat             <  1:8290/149‑1 (MQ=255)
                                                                                                                                 ttAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTtaat             >  1:1032701/1‑149 (MQ=255)
                                                                                                                                         gTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAAtt     >  1:140513/1‑149 (MQ=255)
                                                                                                                                           ataAAATTCTGGGTTTGTCGTCAGTTCAAGTCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCATTCATTTAATAAAGAAtttt   <  2:538573/149‑1 (MQ=255)
                                                                                                                                            taAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTg  >  1:10008/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GCATAATCAATCAAAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTG  >  NC_000913_3_pae_pgi/3354151‑3354439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑