Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi526,7920AC34.9% 72.6 / 81.3 85S1178R (AGC→CGC) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (22/32);  new base C (8/21);  total (32/53)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-02
Rejected as consensus: Frequency below/above cutoff threshold.

GCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGG  >  NC_000913_3_pae_pgi/526647‑526938
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gCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGTCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcg                                                                                                                                                 <  1:156594/149‑1 (MQ=255)
 cgtGCGCGGTGCGGGCTGACGGTGGAGCAACTAGCCAGACAGGTGGAGCCGGAATACACACCGGCGCCAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAgcgc                                                                                                                                                <  2:933748/147‑1 (MQ=255)
 cTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcgg                                                                                                                                                <  1:580537/149‑2 (MQ=255)
  ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcggg                                                                                                                                               <  2:680444/149‑3 (MQ=255)
      gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACCCCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGagt                                                                                                                                           <  1:355279/149‑3 (MQ=255)
       cAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAgta                                                                                                                                          <  2:938187/149‑1 (MQ=255)
        aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGCCTACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                         <  2:704711/149‑1 (MQ=255)
          tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatga                                                                                                                                       <  1:760006/149‑1 (MQ=255)
            tgggCTGACGGTGGAGCAAATGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACGGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgatg                                                                                                                                     <  1:217105/148‑1 (MQ=255)
              ggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGaa                                                                                                                                   <  2:431756/149‑1 (MQ=255)
              ggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAgtat                                                                                                                                         <  1:143085/143‑1 (MQ=255)
              ggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAgtat                                                                                                                                         >  2:143085/1‑143 (MQ=255)
              ggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGaa                                                                                                                                   >  1:593140/1‑149 (MQ=255)
               gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGCTGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                                                  <  1:838404/149‑1 (MQ=11)
                 tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                                <  1:316926/149‑1 (MQ=255)
                 tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                                <  2:667880/149‑1 (MQ=255)
                  gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                               <  2:973253/149‑1 (MQ=255)
                  gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                               <  1:529162/149‑1 (MQ=255)
                  gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCCGCGCGAAAAGCTCATCTTTATCACTGCCACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                               <  2:73129/149‑1 (MQ=11)
                  gACCGTGCAACAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTCATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                               <  2:84893/149‑1 (MQ=255)
                   aCGGTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTACGACCACCGGGGACTGCCGCAGGCGCTCAACAGCAAAGAAGGCAATACGGCCGAGAGCGCGACATCTGATGAATgagg                                                                                                                              >  2:426478/1‑149 (MQ=14)
                    cGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGc                                                                                                                             >  2:22592/1‑149 (MQ=255)
                     ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCa                                                                                                                            >  1:402684/1‑149 (MQ=255)
                       tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                          <  2:152129/149‑1 (MQ=255)
                       tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                          <  1:16420/149‑1 (MQ=255)
                       tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAACTCAACTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                          <  1:142424/149‑1 (MQ=11)
                         gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCa                                                                                                                        <  2:922793/149‑1 (MQ=255)
                           gCACCTGGTCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGAGCGGAATATGATGAATGGGGCAACCAGc                                                                                                                      <  1:211374/149‑1 (MQ=255)
                           gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGc                                                                                                                      <  1:365055/149‑1 (MQ=255)
                            cAACTGGCCAGACGGGTGGAGGCGGAATACACGCCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCt                                                                                                                     <  2:103221/149‑1 (MQ=11)
                               cTGGCCAGACAGGTGGCGACGGTATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTaa                                                                                                                  <  1:149463/149‑1 (MQ=11)
                                tGGCCAGACAGGTCGAACCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                 <  1:836659/149‑1 (MQ=11)
                                   gcggACAGGTGGAGCGGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                              <  2:996775/146‑1 (MQ=11)
                                   ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGAAGAATGAATGGGGCAACCAGCGTAATgag                                                                                                              >  1:543674/1‑149 (MQ=11)
                                    cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                             >  1:48862/1‑149 (MQ=255)
                                    cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCCCTTATCAGCGAAGCCGGCAATACGGCGTGGGGCGCGGAACACATTGAAAGGGGACACCAGCTTACTcagg                                                                                                             >  2:625857/1‑149 (MQ=14)
                                     aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                            <  2:35362/149‑1 (MQ=255)
                                         aGGTGCGGCCGGCATAGACACCGGCGGGAAAAGCTGACTTTTATCACTGGGACCACCGGGGACTGCCGCTTGCGCTTATCAGCGAAGACGGCAATACGTCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                        <  2:63851/149‑1 (MQ=14)
                                          ggTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCAACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAcc                                                                                                       <  1:564810/149‑1 (MQ=11)
                                           gTGGAGTCGGAATACACACCGGCGCGAAAAGCTAATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                      <  1:295089/149‑1 (MQ=11)
                                           gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                      >  1:934891/1‑149 (MQ=255)
                                           gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                      >  1:1016013/1‑149 (MQ=255)
                                            tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTGATAAGCGAAGACGGCAATACGGCGGGGGGCGCGGAATATGATGGAAGGGGCAACCAGCGTCACGGGGAGAAccct                                                                                                     >  2:80231/1‑148 (MQ=14)
                                             ggAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGc                                                                                                    <  1:1042490/149‑1 (MQ=255)
                                              gAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGca                                                                                                   >  1:184637/1‑149 (MQ=255)
                                              gAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGca                                                                                                   <  2:934891/149‑1 (MQ=255)
                                               aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                                  <  1:552241/149‑1 (MQ=255)
                                               aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCCGCGAAGACGGCACTACGGCGGGGCGCCGCGACGATGATGAATGGGGCAACCCGCTTCGGGGGGAGTACCCGcta                                                                                                  >  2:715450/1‑147 (MQ=11)
                                                gtcGGAATACACCCCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                 <  2:542243/147‑1 (MQ=255)
                                                gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACAACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                 >  1:680839/1‑149 (MQ=255)
                                                gCCGGAATACACACCGGCGCCAAAAGCTCATCTTTATCACTGCGACCACCGGTGACTGCCGCTGGCGCTTATTAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                 <  1:432545/149‑1 (MQ=255)
                                                  cGGAACACACACCGGCGCGAAAGGCTCATCTTTATTACGTCGACCACCGGGGACTGCCACTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGCTGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcac                                                                                               <  2:110689/149‑2 (MQ=11)
                                                    gAATACAAAGCGGCGCGAAAAGCTCATGTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                             <  1:16984/149‑1 (MQ=255)
                                                        acccacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCAGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                                         <  2:1045565/146‑1 (MQ=255)
                                                        acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                                         <  2:883982/149‑1 (MQ=255)
                                                          acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                       <  2:931952/149‑1 (MQ=255)
                                                            acCGGCGCGAAAGGCTCATCTTTATCACTGCGACCACCGGGGACTGCGACTGGCGCTTATCAGCGCAGGCGGCGATACGACGTGGAGGGCGGAATATGATAAATGGGGCAACCAGCAAGATGAGGAGAACCGTCATCAtgtgatgtcag                                                                                     >  2:199420/1‑142 (MQ=255)
                                                             ccGGCGCGAAAAGCTCAGCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGcc                                                                                    <  2:816626/149‑1 (MQ=255)
                                                                  gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                               <  2:402684/149‑1 (MQ=255)
                                                                       aaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTg                                                                          >  1:1027222/1‑149 (MQ=255)
                                                                           tCATCTTTATCACTTTGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTc                                                                            >  2:658921/1‑143 (MQ=255)
                                                                           tCATCTTTATCACTGCGACTACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCCTAATGAGCAGAACCCCCATCATGTGTATCAGCCGTCCCGTCTGCCAg                                                                      >  2:888694/1‑149 (MQ=18)
                                                                           tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCACTACGGAGTGGAGCGCGGAATACAATGAATGGGGAAACCAACTTGATGAGGAGGACACGCAACATGTTCCTCATCCGTCCCGtacgccga                                                                      >  2:397212/1‑142 (MQ=255)
                                                                           tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTc                                                                            >  2:658710/1‑143 (MQ=255)
                                                                           tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTc                                                                            <  1:658921/143‑1 (MQ=255)
                                                                           tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTc                                                                            <  1:658710/143‑1 (MQ=255)
                                                                             aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGAGCAACCAGCTTAATGAGGAGAACCCGCATCGTGTGTATCAGCCGTACCGTCTGCCAggg                                                                    >  1:156756/1‑149 (MQ=255)
                                                                                  tATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATAGGGCAACCAGCTTAATGCGGAGAACCCGCAGCATGTGTATCAGCCGTACCGTCTGCCAGGgcagca                                                               >  2:596686/1‑149 (MQ=255)
                                                                                        tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatga                                                         >  1:37478/1‑149 (MQ=255)
                                                                                          ggACCACCGGGGACGGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgatg                                                       <  2:64911/148‑1 (MQ=255)
                                                                                          cGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                               <  1:1003880/77‑1 (MQ=255)
                                                                                          cGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                               >  2:1003880/1‑77 (MQ=255)
                                                                                                  ggggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCa                                               <  2:692754/149‑1 (MQ=255)
                                                                                                     gACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                            <  1:328357/149‑1 (MQ=255)
                                                                                                          ccGCTGGCGCTTATCAGCGAAAACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                       <  1:423279/149‑1 (MQ=255)
                                                                                                           cGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTCATGAGGAGAACCCGCATCATGCGTATCAGCCGTACCGTCTGCCAGGACCGCACCATGATGAGGAATCAGGGCTGTAc                                      >  2:735696/1‑149 (MQ=17)
                                                                                                            gCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACt                                     >  1:700660/1‑149 (MQ=255)
                                                                                                                   cTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAc                                                                                >  1:413644/1‑99 (MQ=255)
                                                                                                                   cTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAc                                                                                <  2:413644/99‑1 (MQ=255)
                                                                                                                    ttATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTTCTATAACCGt                             >  1:47784/1‑149 (MQ=255)
                                                                                                                      aTCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCa                           >  2:607300/1‑149 (MQ=255)
                                                                                                                          gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                      <  2:581006/118‑1 (MQ=255)
                                                                                                                          gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                      >  1:581006/1‑118 (MQ=255)
                                                                                                                             aaGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCTAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCCTCACCGGtac                    >  2:552432/1‑149 (MQ=255)
                                                                                                                             aaGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtac                    <  1:759245/149‑1 (MQ=255)
                                                                                                                                      aTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACta                                    <  2:718877/124‑1 (MQ=255)
                                                                                                                                      aTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACta                                    >  1:718877/1‑124 (MQ=255)
                                                                                                                                               ggAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAgggg  <  2:37478/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGG  >  NC_000913_3_pae_pgi/526647‑526938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑