Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,354,297 G→T intergenic (‑247/‑428) yhcC ← / → gltB putative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,354,2970GT98.5% 216.9 / NA 68intergenic (‑247/‑428)yhcC/gltBputative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (32/34);  minor base C (0/1);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATAATCAATCAAAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTG  >  NC_000913_3_pae_pgi/3354151‑3354439
                                                                                                                                                  |                                                                                                                                              
gCATAATCAATCAAAATTACCGAAATTCCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCt                                                                                                                                              <  1:669618/149‑1 (MQ=255)
 cATAATCAATCAAAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTg                                                                                                                                             <  2:1250811/149‑1 (MQ=255)
 cATAATCAATCAAAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTg                                                                                                                                             >  1:253033/1‑149 (MQ=255)
   tAATCAAACAAAATTACCTAAATTTCATGCATAATCACTTTAAACCCGTCTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGAAAAAAAAAAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGt                                                                                                                                           <  2:438302/149‑1 (MQ=255)
       caatcaatATTACCGAAATTTCAGGCATAATCACATAAATCACTGTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTCATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTg                                                                                                                                       <  2:104548/149‑1 (MQ=255)
       caatcaaAATTCTCGAAATTTCATGCATAATCACATAATTGACTGTTGCTTATCTTGTGTCAGATCTTTTTATCTCCTGATGCATTTTAGGCATAAACAGTAGCATGAAACGTCCTTACCAATTAAGGTAGTATATAATCCTGGTTTTg                                                                                                                                       <  2:1277232/149‑1 (MQ=255)
        aatcaaAATTACCGAAATTTCATGCCTAATCACATAAATCGCTTTTGCGTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTgt                                                                                                                                      <  2:1537518/149‑1 (MQ=255)
         atcaaAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTgtc                                                                                                                                     >  2:1280725/1‑149 (MQ=255)
            aaaaTTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTgtcgtc                                                                                                                                  >  2:473456/1‑149 (MQ=255)
             aaaTTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCa                                                                                                                                 <  1:450645/149‑1 (MQ=255)
                   ccGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCaa                                                                                                                           <  2:290940/149‑1 (MQ=255)
                   ccGAAATTTCACGCATAATCACATAAATCACTTTTGCTTAACTTGTGTCAGATTTTTTTATCTCCTGATTGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCaa                                                                                                                           <  1:600015/149‑1 (MQ=255)
                     ggaaTTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATACTAGTTTTTTCGTCAATTCAAga                                                                                                                         >  1:738546/3‑148 (MQ=255)
                             aTGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATaa                                                                                                                 >  2:1111166/1‑149 (MQ=255)
                             aTGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGATTTTGTCGTCAGTTCAAGGCAGGTTaa                                                                                                                 >  2:1393247/1‑149 (MQ=255)
                               gCATAATCATATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATCGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAgc                                                                                                               <  1:1524993/149‑2 (MQ=255)
                               gCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGGTAAgg                                                                                                               >  1:1349033/1‑149 (MQ=255)
                               gCATAATCACATAAATCACTTTTGCGTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAgg                                                                                                               <  2:1037555/149‑1 (MQ=255)
                               gCAAAATTACATAAATCACTTTTGCTTATCTTGGGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAgg                                                                                                               <  2:760637/149‑1 (MQ=255)
                                  tAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGtt                                                                                                            <  2:100044/149‑1 (MQ=255)
                                    aTCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTaa                                                                                                          <  1:732591/149‑1 (MQ=255)
                                     tCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTTCCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAac                                                                                                         >  1:1448731/1‑149 (MQ=255)
                                       acaTAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGCCAGTATAACATTCTGGTTTAGTCGTCAGTTCAGGGCGCGATAAGGtttgaacc                                                                                                       >  2:315618/1‑144 (MQ=255)
                                                  ttttGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGAc                                                                                            >  1:1546915/1‑149 (MQ=255)
                                                   tttCCTTATCTTGTGTCCGATTAGTTTATCGCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACa                                                                                           <  1:1245720/149‑1 (MQ=255)
                                                       cTTATCTTGTGGCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCACTTAAAGCAGTATAAAATTCTAGTTTTGTCGTCAGTTCAAGGCAGGATAGGGGTTAACACAACTTTATGACACTCa                                                                                       >  2:1187698/1‑149 (MQ=255)
                                                        ttATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAg                                                                                      <  1:1255551/149‑1 (MQ=255)
                                                        ttATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAg                                                                                      <  1:1257003/149‑1 (MQ=255)
                                                         tATCTTGTGTCAGATTCTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAgg                                                                                     <  1:480311/149‑1 (MQ=255)
                                                             ttGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGCCAGAATAAGGTTTAACACACCTTTATGACAGTCAGGAAtt                                                                                 >  1:300698/1‑149 (MQ=255)
                                                             ttGTGTCAGATTTTTTAATCCTCTGATGGAATTTAAGAAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAAtt                                                                                 <  2:253033/149‑1 (MQ=255)
                                                                    aGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGtt                                                                          >  1:1010181/1‑149 (MQ=255)
                                                                    aGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGCCAGGAATTGACTGtt                                                                          >  2:1299859/1‑149 (MQ=255)
                                                                     gATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAGACGTCATGACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGttt                                                                         >  2:413616/1‑149 (MQ=255)
                                                                           tttATCTCCGTATTGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTaa                                                                   <  2:1283060/149‑1 (MQ=255)
                                                                               tctcCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGTTAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGAc                                                               <  1:1061140/149‑1 (MQ=255)
                                                                               tctcCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCAGGACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTCTGACAGTCAGGAATTGACTGTTTCTATaagcac                                                               >  1:728831/1‑145 (MQ=255)
                                                                                 tcCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACtt                                                             <  1:1111166/149‑1 (MQ=255)
                                                                                 tcCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACtt                                                             >  1:73840/1‑149 (MQ=255)
                                                                                   cTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTcc                                                           >  2:1053673/1‑149 (MQ=255)
                                                                                      aTGGATTTTAGGCAAAAACAGTAGCATGACACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCtt                                                        <  2:413086/149‑1 (MQ=255)
                                                                                      aTGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTACCGACTTCCCtt                                                        >  2:1444460/1‑149 (MQ=255)
                                                                                           ttttAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGc                                                   >  1:1555479/1‑149 (MQ=255)
                                                                                           ttttAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGc                                                   >  2:1507313/1‑149 (MQ=255)
                                                                                                 acAAAAACAGTATGATGAAACGTCATCAACAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCATTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTaaa                                             <  2:72866/148‑1 (MQ=255)
                                                                                                    aaaaCAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGAt                                          <  2:791080/149‑1 (MQ=255)
                                                                                                             gCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGCCCGACATCTCAGGATTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCAt                                 >  1:47024/1‑149 (MQ=255)
                                                                                                             gCATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCAt                                 <  2:125569/149‑1 (MQ=255)
                                                                                                              cATGAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCAtt                                >  1:803633/1‑149 (MQ=255)
                                                                                                                 gAAACGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTa                             >  2:441957/1‑149 (MQ=255)
                                                                                                                  aaaCGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGAAATTGACTGTTTCTCTAACGACATCCCTTTTAGCCTGAAAGATAAAATCCATTTTaa                            <  2:955543/149‑1 (MQ=255)
                                                                                                                   aaCGTCATTACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAAt                           >  1:59437/1‑149 (MQ=255)
                                                                                                                       tCATTACCAATTAAGGCAGTATAGAATTCTGGTTTTGTCGTCAGTACAAGGCAGGATAAGGGTTAACACACCTTGATGACAGTCAGGAAGTGACTGTTTCTCTCTCGCCTTCCCTTTTGGCCTTAAAGAAAAAATCCATTTTAATTTCa                       <  2:85510/149‑1 (MQ=255)
                                                                                                                          ttACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTccc                                                          >  2:308383/1‑111 (MQ=255)
                                                                                                                          ttACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTccc                                                          <  1:308383/111‑1 (MQ=255)
                                                                                                                          ttACCAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTc                    >  2:105359/1‑149 (MQ=255)
                                                                                                                             ccAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCa                                                                                                                       >  2:247558/1‑47 (MQ=255)
                                                                                                                             ccAATTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCa                                                                                                                       <  1:247558/47‑1 (MQ=255)
                                                                                                                               aaTTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTaaa                                             <  1:569053/119‑1 (MQ=255)
                                                                                                                               aaTTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTaaa                                             >  2:569053/1‑119 (MQ=255)
                                                                                                                                aTTAAGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTtaa              >  1:276396/1‑149 (MQ=255)
                                                                                                                                   aaGGCCGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTtaataa           <  1:473456/149‑1 (MQ=255)
                                                                                                                                   aaGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATTTATTCTAAATCCCGTCATTtaataa           >  2:332510/1‑149 (MQ=255)
                                                                                                                                    aGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATaaa          >  1:4203/1‑149 (MQ=255)
                                                                                                                                    aGGCAGTATAAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATaaa          <  1:902418/149‑1 (MQ=255)
                                                                                                                                            taAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCt                                                 >  1:813600/1‑102 (MQ=255)
                                                                                                                                            taAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCt                                                 <  2:813600/102‑1 (MQ=255)
                                                                                                                                            taAAATTCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTg  <  1:413616/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GCATAATCAATCAAAATTACCGAAATTTCATGCATAATCACATAAATCACTTTTGCTTATCTTGTGTCAGATTTTTTTATCTCCTGATGGATTTTAGGCAAAAACAGTAGCATGAAACGTCATTACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTG  >  NC_000913_3_pae_pgi/3354151‑3354439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑