Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 4,234,667 Δ1 bp intergenic (‑1434/‑499) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,234,6660A.99.3% 686.2 / ‑2.1 145intergenic (‑1433/‑500)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (75/68);  minor base C (1/0);  total (76/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

GGGTCTATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATT  >  NC_000913_3_pae_pgi/4234517‑4234809
                                                                                                                                                     |                                                                                                                                               
     tatataCGCAGTAGCGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGGCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatcatc                                                                                                                                           <  3:1016381/150‑1 (MQ=255)
        ataCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgtc                                                                                                                                        <  1:995040/150‑1 (MQ=255)
        ataCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACCGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgtc                                                                                                                                        <  3:567967/150‑1 (MQ=255)
         taCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgtcg                                                                                                                                       >  1:1414443/1‑150 (MQ=255)
          aCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGa                                                                                                                                      >  3:149356/1‑150 (MQ=255)
          aCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGa                                                                                                                                      <  1:1331946/150‑1 (MQ=255)
            gCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGata                                                                                                                                    >  1:810583/1‑150 (MQ=255)
            gCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGata                                                                                                                                    <  1:1308980/150‑1 (MQ=255)
             cAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGatat                                                                                                                                   >  3:904256/1‑150 (MQ=255)
              aGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATg                                                                                                                                  >  1:1271301/1‑150 (MQ=255)
               gTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGt                                                                                                                                 >  1:1335340/1‑150 (MQ=255)
               gTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGt                                                                                                                                 >  4:539342/1‑150 (MQ=255)
                  gagaTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAgg                                                                                                                              <  4:904256/150‑1 (MQ=255)
                  gagaTGCACCCCAAGGCCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAgg                                                                                                                              >  2:858747/1‑150 (MQ=255)
                   agaTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGt                                                                                                                                 <  1:1020810/146‑1 (MQ=255)
                   agaTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGt                                                                                                                                 >  2:1020810/1‑146 (MQ=255)
                     aTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAGACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCg                                                                                                                           >  3:850672/1‑150 (MQ=255)
                     aTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCg                                                                                                                           <  3:531833/150‑1 (MQ=255)
                     aTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCg                                                                                                                           >  1:281308/1‑150 (MQ=255)
                       gCACCCCAAGGGCGTTTTATCCTTTTTTCGATATGTTGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATTGATGCATTGACGAGGCGCTTGCAGTGATCATCTTCGATATGTAGGCCGGa                                                                                                                         >  2:541115/1‑150 (MQ=255)
                       gCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGa                                                                                                                         >  1:1163921/1‑150 (MQ=255)
                       gCACCCCAAGGGCGTTATATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTGGCAGTGATCATCGTCCATAAGTAGGCGGGa                                                                                                                         >  2:1421914/1‑150 (MQ=255)
                        cACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGAt                                                                                                                        >  3:200227/1‑150 (MQ=255)
                          ccccAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATaa                                                                                                                      <  1:272529/150‑1 (MQ=255)
                          ccccAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATaa                                                                                                                      >  1:1203235/1‑150 (MQ=255)
                          ccccAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATaa                                                                                                                      >  1:420228/1‑150 (MQ=255)
                           cccAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGTCAGCGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                     >  2:618721/1‑150 (MQ=255)
                           cccAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGa                                                                                                                         >  4:512/1‑146 (MQ=255)
                           cccAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGa                                                                                                                         <  3:512/146‑1 (MQ=255)
                             cAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                                   >  1:702848/1‑150 (MQ=255)
                                 ggCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCATAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTc                                                                                                               >  1:53094/1‑150 (MQ=255)
                                        tATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgccgc                                                                                                        <  2:703027/150‑1 (MQ=255)
                                          tCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                                      <  4:700819/150‑1 (MQ=255)
                                             tttttACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                   >  4:178176/1‑150 (MQ=255)
                                             tttttACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                   >  3:253179/1‑150 (MQ=255)
                                             tttttACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGAGTAGAGGCGTTAATCCCCTGGCCACCCCGAGAATAGATGCAGTGAAGAGGCGCTCGCAGTGATCATCGACGATATGTAGGCCGGCGAAGGCGTTCACGCCGCATCCg                                                                                                   >  2:1618150/1‑150 (MQ=255)
                                                      gaTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                          <  1:1132517/150‑1 (MQ=255)
                                                       aTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCTCCTGGCCACCCCGAGAATGGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATg                                                                                         >  1:1476261/1‑150 (MQ=255)
                                                       aTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATg                                                                                         <  2:291333/150‑1 (MQ=255)
                                                        tgtgGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                        <  2:1107588/150‑1 (MQ=255)
                                                         gtgGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                    >  1:387421/1‑137 (MQ=255)
                                                         gtgGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                    <  2:387421/137‑1 (MQ=255)
                                                           gggCATCGCCTCTGTAGAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCTGCATCCGGCAACCGATGCCtt                                                                                     >  2:861835/1‑149 (MQ=255)
                                                            ggCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                                    <  2:94255/150‑1 (MQ=255)
                                                              cATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg                                                                                  <  2:1326590/150‑1 (MQ=255)
                                                                 cGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                               <  1:1523551/150‑1 (MQ=255)
                                                                    ctctGTAAAACTGGCAGAGATTAGAGGCGTAGATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgc                                                                            >  1:891817/1‑150 (MQ=255)
                                                                        gTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                        <  3:221522/150‑1 (MQ=255)
                                                                         tagaaCTGGCAGAGGTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGACGAGGCGCTTGCGGTGAGCAACGTCGATATGTAGGCCGGATAACGCGCTCGCCCCCCATCCCGCAAACGATACCTGATGCGACGCGGTcc                                                                       >  2:1122234/4‑149 (MQ=255)
                                                                         tAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcg                                                                       >  3:708383/1‑150 (MQ=255)
                                                                          aaaaCTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                      >  2:274948/1‑150 (MQ=255)
                                                                                ggCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                <  2:95529/150‑1 (MQ=255)
                                                                                 gCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGGATAGATTCAGTTAAGAGGCGCTTGCAGGGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                               <  1:1421914/150‑1 (MQ=37)
                                                                                   agagATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                             >  1:1192014/1‑150 (MQ=255)
                                                                                    gagaTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGa                                                                                                                         <  4:7533/89‑1 (MQ=255)
                                                                                     agaTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAgg                                                           <  2:420228/150‑1 (MQ=38)
                                                                                       aTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                         >  3:63528/1‑150 (MQ=255)
                                                                                        ttAGATGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAGGcat                                                        <  1:1624715/150‑3 (MQ=37)
                                                                                        ttAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAGGcat                                                        <  1:1624152/150‑3 (MQ=38)
                                                                                         tAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa                                                       <  3:603565/150‑1 (MQ=255)
                                                                                            agGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                                    >  1:525742/1‑150 (MQ=255)
                                                                                             ggCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgtcg                                                                                                                                       <  4:93031/66‑1 (MQ=255)
                                                                                              gCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                >  4:216927/1‑136 (MQ=255)
                                                                                              gCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                <  3:216927/136‑1 (MQ=255)
                                                                                              gCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa                                                       <  4:292559/145‑1 (MQ=255)
                                                                                              gCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa                                                       >  3:292559/1‑145 (MQ=255)
                                                                                              gCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                  >  4:443568/1‑150 (MQ=255)
                                                                                              gCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                  >  3:724102/1‑150 (MQ=255)
                                                                                              gCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                  >  2:1555798/1‑150 (MQ=255)
                                                                                               cGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgc                                                                            <  2:1485584/123‑1 (MQ=255)
                                                                                               cGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgc                                                                            >  1:1485584/1‑123 (MQ=255)
                                                                                                gTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                     <  3:999017/97‑1 (MQ=255)
                                                                                                    aTCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                            <  2:1175699/150‑1 (MQ=255)
                                                                                                     tCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                           <  3:45040/150‑1 (MQ=255)
                                                                                                       cccTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGca                                                         >  1:797761/1‑133 (MQ=255)
                                                                                                       cccTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGca                                                         <  2:797761/134‑2 (MQ=255)
                                                                                                       cccTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                         >  3:230745/1‑150 (MQ=255)
                                                                                                         cTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                   >  2:1315525/1‑90 (MQ=255)
                                                                                                         cTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                   <  1:1315525/90‑1 (MQ=255)
                                                                                                              cACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                  >  2:505218/1‑150 (MQ=255)
                                                                                                              cACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                  <  2:1271301/150‑1 (MQ=255)
                                                                                                              cACCCCGAAAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                  <  1:1674288/150‑1 (MQ=255)
                                                                                                                  ccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg                              <  4:850672/150‑1 (MQ=255)
                                                                                                                     agaATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAt                           <  2:1192014/150‑1 (MQ=255)
                                                                                                                      gaATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                          <  1:618721/150‑1 (MQ=255)
                                                                                                                          aGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg                                          >  4:1024720/1‑130 (MQ=255)
                                                                                                                          aGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg                                          <  3:1024720/130‑1 (MQ=255)
                                                                                                                            aTGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                    >  2:469725/1‑150 (MQ=255)
                                                                                                                             tGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt                   <  2:59793/150‑1 (MQ=255)
                                                                                                                                 ttGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                     >  3:784445/1‑64 (MQ=255)
                                                                                                                                  tGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa              >  2:1274828/1‑150 (MQ=255)
                                                                                                                                   gAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc             >  4:556516/1‑150 (MQ=255)
                                                                                                                                    aaGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACa            >  4:820010/1‑150 (MQ=255)
                                                                                                                                      gagGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                          <  4:655380/102‑1 (MQ=255)
                                                                                                                                      gagGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                          >  3:655380/1‑102 (MQ=255)
                                                                                                                                       agGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAg         >  1:363734/1‑150 (MQ=255)
                                                                                                                                         gcgcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa       >  3:868134/1‑150 (MQ=255)
                                                                                                                                         gcgcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa       >  1:1547232/1‑150 (MQ=255)
                                                                                                                                            cTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACga    <  1:1274828/150‑1 (MQ=255)
                                                                                                                                              tGCAGTGATCATCGTCGACACGTTCGCCGGATAAGGCCTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt  <  1:861835/150‑1 (MQ=37)
                                                                                                                                                     |                                                                                                                                               
GGGTCTATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATT  >  NC_000913_3_pae_pgi/4234517‑4234809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: