Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,731,621 G→T M845I (ATG→ATT lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,731,6210GT99.3% 497.5 / NA 135M845I (ATG→ATTlhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (60/74);  total (61/74)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

ATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTA  >  NC_000913_3_pae_pgi/1731476‑1731765
                                                                                                                                                 |                                                                                                                                                
aTCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                              <  1:666494/150‑1 (MQ=255)
aTCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                              <  3:212510/150‑1 (MQ=255)
 tCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATtcgtcg                                                                                                                                             <  1:645348/150‑1 (MQ=255)
  cGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGc                                                                                                                                            <  4:769629/150‑1 (MQ=255)
      ccccGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGa                                                                                                                                        <  2:302604/150‑1 (MQ=255)
       cccGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAGTCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGGATGTCTGCAAGATGGTTATGATCTTCGCGCACTGGAACGCTTGATGCGTCGCCGGaa                                                                                                                                       >  2:1241209/1‑150 (MQ=255)
            tttGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTg                                                                                                                                  <  4:918572/150‑1 (MQ=255)
             ttGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTgg                                                                                                                                 <  2:1161614/150‑1 (MQ=255)
                       cGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaa                                                                                                                       <  3:831503/150‑1 (MQ=255)
                       cGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaa                                                                                                                       >  1:286613/1‑150 (MQ=255)
                       cGGCTGCGCGCCAGTCAGGTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaa                                                                                                                       <  4:579661/150‑1 (MQ=255)
                        ggCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGc                                                                                                                                            <  4:42561/128‑1 (MQ=255)
                        ggCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGc                                                                                                                                            >  3:42561/1‑128 (MQ=255)
                        ggCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                      <  4:634088/150‑1 (MQ=255)
                        ggCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                      >  3:961483/1‑150 (MQ=255)
                          cTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAata                                                                                                                    >  2:278539/1‑150 (MQ=255)
                                gcCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAt                                                                                                              >  4:11188/1‑150 (MQ=255)
                                gcCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAt                                                                                                              <  2:825286/150‑1 (MQ=255)
                                  cagtcagtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGt                                                                                                            <  1:862684/150‑1 (MQ=255)
                                    gtcagtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTaa                                                                                                          <  1:461871/150‑1 (MQ=255)
                                    gtcagtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTaa                                                                                                          <  1:1041394/150‑1 (MQ=255)
                                        gtTGCTGGATTTCGTTCAGGGATATCCGGATTTTCCGGTCATTCTTGAAACCCTACGCGAATGTCTGCAAGATGTTTATGCTCTTTCCGCACTGGAACGTTTGATTCGTCGCCTGATCGGTGGCGAAATTCAAATATCCGATGTAACGAc                                                                                                      <  2:798735/150‑1 (MQ=255)
                                        gtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAc                                                                                                      <  1:923791/150‑1 (MQ=255)
                                         ttGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAcc                                                                                                     >  1:635592/1‑150 (MQ=255)
                                           gCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCAc                                                                                                   >  2:653170/1‑150 (MQ=255)
                                             tGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGt                                                                                                                                               >  4:64413/1‑104 (MQ=255)
                                             tGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGt                                                                                                                                               <  3:64413/104‑1 (MQ=255)
                                                   tCGCTCAGGGATATCCGCGGTTTCGGGTCATTCTCGAGACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCt                                                                                           <  1:822083/150‑1 (MQ=255)
                                                     gCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                      >  3:24154/1‑121 (MQ=255)
                                                      cTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                              <  2:1547925/96‑1 (MQ=255)
                                                      cTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                              >  1:1547925/1‑96 (MQ=255)
                                                      cTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGc                                                                                        <  1:424321/150‑1 (MQ=255)
                                                       tCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGcc                                                                                       <  3:745151/150‑1 (MQ=255)
                                                         aGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                      <  1:225885/117‑1 (MQ=255)
                                                         aGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                      >  2:225885/1‑117 (MQ=255)
                                                         aGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCAc                                                                                                   >  2:1269440/1‑136 (MQ=255)
                                                         aGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCAc                                                                                                   <  1:1269440/136‑1 (MQ=255)
                                                          gggATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCttt                                                                                    <  1:1125905/150‑1 (MQ=255)
                                                          gggATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTAGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCttt                                                                                    >  4:392731/1‑150 (MQ=255)
                                                           ggATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTc                                                                                   <  3:895391/150‑1 (MQ=255)
                                                             atatCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGc                                                                                 >  4:307699/1‑150 (MQ=255)
                                                                tCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCcac                                                                              >  1:1488023/1‑150 (MQ=255)
                                                                 ccGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                      >  1:49741/1‑109 (MQ=255)
                                                                 ccGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                      <  2:49741/109‑1 (MQ=255)
                                                                  cGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACaa                                                                            >  3:213851/1‑150 (MQ=255)
                                                                    gATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCTTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGt                                                                          <  4:961483/150‑1 (MQ=255)
                                                                       tttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTa                                                                       >  3:882264/1‑150 (MQ=255)
                                                                         tCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTAtt                                                                     <  4:882264/150‑1 (MQ=255)
                                                                                aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGc                                                                                 <  4:591251/131‑1 (MQ=255)
                                                                                aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGc                                                                                 >  3:591251/1‑131 (MQ=255)
                                                                                aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc                                                              <  1:180815/150‑1 (MQ=255)
                                                                                  tctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTa                                                            >  1:677191/1‑150 (MQ=255)
                                                                                  tctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTa                                                            >  1:1203856/1‑150 (MQ=255)
                                                                                   ctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAAGATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTAt                                                           <  1:928210/150‑1 (MQ=255)
                                                                                    tcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGc                                                                                        >  2:89131/1‑120 (MQ=255)
                                                                                    tcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGc                                                                                        <  1:89131/120‑1 (MQ=255)
                                                                                      gAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTc                                                        >  2:943524/1‑150 (MQ=255)
                                                                                         aCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTcgcg                                                     <  4:213851/150‑1 (MQ=255)
                                                                                         aCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTcgcg                                                     <  2:1475371/150‑1 (MQ=255)
                                                                                            cTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCtt                                                                                     >  4:595625/1‑115 (MQ=255)
                                                                                            cTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCtt                                                                                     <  3:595625/115‑1 (MQ=255)
                                                                                               cgcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAt                                                                                                              <  3:970705/87‑1 (MQ=255)
                                                                                               cgcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAt                                                                                                              >  4:970705/1‑87 (MQ=255)
                                                                                                gcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGc                                                                                                                                >  2:952504/1‑68 (MQ=255)
                                                                                                gcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTa                                              >  1:327146/1‑150 (MQ=255)
                                                                                                 cgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCGCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTAt                                             <  1:1287091/150‑1 (MQ=255)
                                                                                                  gAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGc                                                                                        <  2:1052240/106‑1 (MQ=255)
                                                                                                  gAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATg                                            <  2:1284902/150‑1 (MQ=255)
                                                                                                  gAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATg                                            >  1:779601/1‑150 (MQ=255)
                                                                                                  gAATGTCTGCAAGATGGTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCGGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCGTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATg                                            <  2:1072422/150‑1 (MQ=255)
                                                                                                   aaTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGt                                           <  1:1114420/150‑1 (MQ=255)
                                                                                                   aaTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGt                                           <  2:779601/150‑1 (MQ=255)
                                                                                                     tGTCTGCAAGATGTTTCTGATCTGCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTAc                                         <  1:1221185/150‑1 (MQ=255)
                                                                                                     tGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTAc                                         <  2:1326974/150‑1 (MQ=255)
                                                                                                       tCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCa                                       <  2:635592/150‑1 (MQ=255)
                                                                                                           cAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGttt                                                                        <  4:934157/113‑1 (MQ=255)
                                                                                                            aaGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCg                                                                <  4:972030/120‑1 (MQ=255)
                                                                                                            aaGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCg                                                                >  3:972030/1‑120 (MQ=255)
                                                                                                               aTGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACg                               >  4:129240/1‑150 (MQ=255)
                                                                                                                   ttATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTc                                                                 <  3:521517/112‑1 (MQ=255)
                                                                                                                    tATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAAt                                                 >  3:899014/1‑127 (MQ=255)
                                                                                                                    tATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGt                                           >  1:1506969/1‑133 (MQ=255)
                                                                                                                    tATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGt                                           <  2:1506969/133‑1 (MQ=255)
                                                                                                                    tATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCg                          >  1:1095547/1‑150 (MQ=255)
                                                                                                                       gATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGccc                            <  2:90071/145‑1 (MQ=255)
                                                                                                                        aTCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACTAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTgg                      <  1:943524/150‑1 (MQ=255)
                                                                                                                           ttCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaa                                                                                                                       <  4:733867/50‑1 (MQ=255)
                                                                                                                           ttCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCag                   >  1:1012273/1‑150 (MQ=255)
                                                                                                                            tCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCaga                  <  2:78254/150‑1 (MQ=255)
                                                                                                                              ccGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCcac                                                                              >  2:1547225/1‑88 (MQ=255)
                                                                                                                               cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTAc                                         >  2:1320943/1‑124 (MQ=255)
                                                                                                                               cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTAc                                         <  1:1320943/124‑1 (MQ=255)
                                                                                                                                  aCTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCg            <  2:327146/150‑1 (MQ=255)
                                                                                                                                   cTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCa                                       <  2:1005855/122‑1 (MQ=255)
                                                                                                                                   cTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCa                                       >  1:1005855/1‑122 (MQ=255)
                                                                                                                                   cTGGAACGTTTGACTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCgg           <  2:281674/150‑1 (MQ=255)
                                                                                                                                    tGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCggg          >  3:514275/1‑150 (MQ=255)
                                                                                                                                      gAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCa        >  1:504881/1‑150 (MQ=255)
                                                                                                                                       aaCGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGc                                                                                              <  2:1292101/63‑1 (MQ=255)
                                                                                                                                       aaCGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGc                                                                                              >  1:1292101/1‑63 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTA  >  NC_000913_3_pae_pgi/1731476‑1731765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: