| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_pae_pgi | = 4162839 | 18 (1.040) | 3 (0.180) | 3/258 | 2.1 | 14.3% | coding (432/1101 nt) | trmA | tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase |
| ? | NC_000913_3_pae_pgi | = 4162849 | 19 (1.160) | coding (422/1101 nt) | trmA | tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase | |||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
TTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCC > NC_000913_3_pae_pgi/4162740‑4162992 | tttCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAg > 4:65714/1‑150 (MQ=255) cccTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGctt < 2:62797/150‑1 (MQ=255) ccGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATcgcgcagaatctgaatgtgcatctgattggtcgggcaacgaaaaccaaaat > 3:50497‑M2/1‑99 (MQ=255) gCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGtata < 3:64236/150‑1 (MQ=255) tCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGaa < 2:18491/150‑1 (MQ=255) aTCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAac > 1:154408/1‑150 (MQ=255) aTGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATCCCAGGGCAaccacc > 4:112503/1‑150 (MQ=255) ccTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGAt < 3:65714/150‑1 (MQ=255) ttGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAgg > 1:103149/1‑107 (MQ=255) ttGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAgg < 2:103149/107‑1 (MQ=255) gCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTgg > 2:148253/1‑150 (MQ=255) ccGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGaa > 1:53752/1‑150 (MQ=255) aTCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATcgcgca < 2:18307‑M2/43‑7 (MQ=255) aTCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATcgcgca > 1:18307‑M2/1‑37 (MQ=255) tCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATcgcgcagaatctgaatgtgcatctgattggtcgggcaacgaaaaccaaaatcgagctggatcaggattacat < 2:55704‑M2/108‑73 (MQ=255) tCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATcgcgcagaatctgaatgtgcatctgattggtcgggcaacgaaaaccaaaatcgagctggatcaggattacat > 1:55704‑M2/1‑36 (MQ=255) acaTTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGgcgc < 1:86159/147‑1 (MQ=255) acaTTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGgcgc > 2:86159/1‑147 (MQ=255) tCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACgg > 2:192902/1‑150 (MQ=255) aGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGa > 2:77591/1‑150 (MQ=255) tCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGattat < 2:154408/150‑1 (MQ=255) gcgcgcgcAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACt > 1:102450/1‑94 (MQ=255) gcgcgcgcAGTGCATCGCGCAGGGCCTCCGCTTCCAGACCCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACt < 2:102450/94‑1 (MQ=255) aGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACAccc > 1:45869/1‑150 (MQ=255) | TTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACCC > NC_000913_3_pae_pgi/4162740‑4162992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |