Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 2,867,260 T→G N98H (AAC→CAC)  rpoS ← RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi2,867,2600TG99.3% 986.6 / ‑2.3 282N98H (AAC→CAC) rpoSRNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (151/128);  total (153/129)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

Only 100 of 282 total aligned reads displayed.

CGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTC  >  NC_000913_3_pae_pgi/2867116‑2867406
                                                                                                                                                |                                                                                                                                                  
cGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGActct                                                                                                                                               >  1:1528171/1‑150 (MQ=255)
 gTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACtctc                                                                                                                                              <  4:759083/150‑1 (MQ=255)
 gTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACtctc                                                                                                                                              <  1:793290/150‑1 (MQ=255)
   aTGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGa                                                                                                                                            <  2:904066/150‑1 (MQ=255)
      ttGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGatca                                                                                                                                         >  1:1569189/1‑150 (MQ=255)
      ttGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGatca                                                                                                                                         >  3:611/1‑150 (MQ=255)
        gagaAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCACTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGatcatc                                                                                                                                       >  2:638217/1‑150 (MQ=255)
         agaAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATcc                                                                                                                                      >  4:876027/1‑150 (MQ=255)
               ggAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCggcggc                                                                                                                                <  3:967083/150‑1 (MQ=255)
                gAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGgcggcg                                                                                                                               <  4:1124732/150‑1 (MQ=255)
                gAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGgcggcg                                                                                                                               >  1:1919943/1‑150 (MQ=255)
                gAAACCACGTTCCGGGTCAAACTTCNCTACCGCGCGGANCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCACTTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGgcggcg                                                                                                                               >  3:1060285/1‑150 (MQ=255)
                    ccACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGagag                                                                                                                           <  1:1828548/150‑1 (MQ=255)
                     cACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCg                                                                                                                                             <  2:2292210/131‑1 (MQ=255)
                     cACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCg                                                                                                                                             >  1:2292210/1‑131 (MQ=255)
                     cACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAgg                                                                                                                          >  1:295291/1‑150 (MQ=255)
                     cACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAgg                                                                                                                          >  3:355457/1‑150 (MQ=255)
                      aCGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGagag                                                                                                                           >  3:1428157/1‑148 (MQ=255)
                        gTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGa                                                                                                                       >  3:277383/1‑150 (MQ=255)
                             gggTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGAc                                                                                                                                                  <  4:681889/118‑1 (MQ=255)
                             gggTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACAtct                                                                                                                  >  2:302908/1‑150 (MQ=255)
                              ggTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACAtctc                                                                                                                 <  1:516264/150‑1 (MQ=255)
                               gTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTcc                                                                                                                >  2:18248/1‑150 (MQ=255)
                                 cAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCAc                                                                                                              <  1:679736/150‑1 (MQ=255)
                                 cAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCAc                                                                                                              <  2:1005967/150‑1 (MQ=255)
                                 cAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCAc                                                                                                              <  4:611/150‑1 (MQ=255)
                                         tctACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCcggcgg                                                                                                                                 <  3:368805/123‑1 (MQ=255)
                                           tACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGcgcg                                                                                                    >  3:441730/1‑150 (MQ=255)
                                           tACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGcgcg                                                                                                    >  1:2001765/1‑150 (MQ=255)
                                             ccGCGCGGATCAGCCCCAGGTTGCCGTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGAc                                                                                                  <  3:1351597/150‑1 (MQ=255)
                                             ccGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGAc                                                                                                  <  3:572441/150‑1 (MQ=255)
                                               gcgcgGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGatc                                                                                                                                          <  2:1349189/108‑1 (MQ=255)
                                               gcgcgGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCAc                                                                                                              >  2:560679/1‑136 (MQ=255)
                                                       cAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTgcgc                                                                                                     <  2:178422/137‑1 (MQ=255)
                                                       cAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTgcgc                                                                                                     >  1:178422/1‑137 (MQ=255)
                                                        aGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACCCGCAAAATaa                                                                                       <  4:285072/150‑1 (MQ=255)
                                                         gCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTgcg                                                                                                      >  2:2218198/1‑134 (MQ=255)
                                                          ccccAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCACAATAAAc                                                                                     >  2:2004039/1‑150 (MQ=255)
                                                          ccccAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAAc                                                                                     <  1:57321/150‑1 (MQ=255)
                                                            ccAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAActt                                                                                   >  4:1153804/1‑150 (MQ=255)
                                                             cAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATaaa                                                                                      >  2:516086/1‑146 (MQ=255)
                                                             cAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAActtc                                                                                  <  3:649677/150‑1 (MQ=255)
                                                                 ttGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGTCTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCTAAATGAActtcttct                                                                              <  4:1220194/150‑1 (MQ=255)
                                                                     ccTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCa                                                                                                           <  3:1027235/117‑1 (MQ=255)
                                                                     ccTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCgg                                                                          <  2:1569189/150‑1 (MQ=255)
                                                                     ccTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCgg                                                                          >  1:1479824/1‑150 (MQ=255)
                                                                      ctctTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAActt                                                                                   >  4:1265823/1‑140 (MQ=255)
                                                                       tctTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGa                                                                                                                       >  4:1385/1‑103 (MQ=255)
                                                                       tctTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGcc                                                                        >  2:1063479/1‑150 (MQ=255)
                                                                         ttCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGt                                                                      <  3:120066/150‑1 (MQ=255)
                                                                          tCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGa                                                                                                                                            <  1:2217850/79‑1 (MQ=255)
                                                                           cGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTa                                                                    >  3:1184580/1‑150 (MQ=255)
                                                                            gATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGAc                                                                                                                      <  2:1918832/99‑1 (MQ=255)
                                                                            gATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTaa                                                                   >  4:947013/1‑150 (MQ=255)
                                                                            gATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTaa                                                                   >  2:1048211/1‑150 (MQ=255)
                                                                                 ggTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtg                                                              <  3:863036/150‑1 (MQ=255)
                                                                                   tCCAGCAACGCCAGACCACGATTGCCATAACGGCGGTCAATTTTTACCACCAGACGCAAGCGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCg                                                                       <  2:1627477/139‑1 (MQ=255)
                                                                                   tCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                            >  1:1006211/1‑150 (MQ=255)
                                                                                   tCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                            <  1:319568/150‑1 (MQ=255)
                                                                                   tCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                            >  1:1039143/1‑150 (MQ=255)
                                                                                     cAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                            <  2:516719/148‑1 (MQ=255)
                                                                                      aGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGAcg                                                                                                 <  1:1323088/110‑1 (MQ=255)
                                                                                      aGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGaa                                                         <  1:1647272/150‑1 (MQ=255)
                                                                                        cAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCaaa                                                                                           <  3:1135830/114‑1 (MQ=255)
                                                                                            gCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggtctggc                                                       <  4:395019/146‑6 (MQ=255)
                                                                                            gCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCa                                                   <  2:231556/150‑1 (MQ=255)
                                                                                              cAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAAt                                                 >  2:1121792/1‑150 (MQ=255)
                                                                                                gACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                            >  2:498873/1‑137 (MQ=255)
                                                                                                gACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                            <  1:498873/137‑1 (MQ=255)
                                                                                                 aCCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGActc                                                                                                                                                <  2:1682057/52‑1 (MQ=255)
                                                                                                 aCCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGActc                                                                                                                                                >  1:1682057/1‑52 (MQ=255)
                                                                                                 aCCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAAtctc                                              >  2:521126/1‑150 (MQ=255)
                                                                                                      gATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCaa                                         <  4:441730/150‑1 (MQ=255)
                                                                                                             aTAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAATGTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAg                                  >  3:1432314/1‑150 (MQ=255)
                                                                                                             aTAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAg                                  >  1:463071/1‑150 (MQ=255)
                                                                                                              tAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGc                                 >  1:2169646/1‑150 (MQ=255)
                                                                                                              tAACGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGc                                 >  3:402048/1‑150 (MQ=255)
                                                                                                               aaCGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAActtcttctt                                                                             <  1:2384485/105‑1 (MQ=255)
                                                                                                               aaCGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCt                                <  4:628431/150‑1 (MQ=255)
                                                                                                               aaCGGCGGGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCt                                <  2:1390304/150‑1 (MQ=255)
                                                                                                                 cggcggGCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGa                              <  2:875771/150‑1 (MQ=255)
                                                                                                                 cggcggGCAATTTTTACCACCAGACGCAAGTGACTCCCGATCACCCGGCGGCGACAGGCGACATCTCCACGCCGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTCACAGTGGTGAATAACCACTCTCACCAAGGTAAAGCTgc                              >  2:761188/1‑149 (MQ=255)
                                                                                                                    ggggCAATTTTTACCACGAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTc                           <  2:1439832/149‑1 (MQ=255)
                                                                                                                      ggCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAAc                                                                                     >  2:1231200/1‑90 (MQ=255)
                                                                                                                       gCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTcgcg                        >  4:361963/1‑150 (MQ=255)
                                                                                                                       gCAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTcgcg                        <  2:2159718/150‑1 (MQ=255)
                                                                                                                        cAATTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTcgcgg                       >  1:931286/1‑149 (MQ=255)
                                                                                                                         aaTTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTc                      >  1:1376789/1‑150 (MQ=255)
                                                                                                                          aTTTTTACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTcc                     <  4:1429286/150‑1 (MQ=255)
                                                                                                                          aTTTCTACCACCAGACGCAAGTCACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATACCCAATCTCACCAAGGTAAAGCTGAGTCGCGTcc                     <  4:1051022/150‑1 (MQ=255)
                                                                                                                           tttttACCACCAGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGt                       >  1:1846642/1‑147 (MQ=255)
                                                                                                                             tttACCACCAGACGCAAGGGACTCGCGATCGTCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTACTGCGGCCGTTAACAGTGGTGAATAACCATTCTCACCAAGGTAAAGCTGAGTCGTGTCCAac                  <  2:2373717/150‑1 (MQ=255)
                                                                                                                                 ccaccaGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacacac              <  1:1121792/150‑1 (MQ=255)
                                                                                                                                   accaGACGCAAGTGACTCTCGATCATCCGGCGGCGCGAGGCGACATCTCCACGCAGTGCGAGACGCGCAAAATAAACTTCTTCTTCGGCCGTTTACAGTGGTGAATAACCAATCTCACCAAGGGAAAGCTGAGTCGCGTCCATAACACGc            >  4:180219/1‑150 (MQ=255)
                                                                                                                                    ccaGACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCt           >  1:875973/1‑150 (MQ=255)
                                                                                                                                       gACGCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgtg        >  2:1471942/1‑150 (MQ=255)
                                                                                                                                          gCAAGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCATCCAACACACGCTGTGTgg     <  1:521126/150‑1 (MQ=255)
                                                                                                                                            aaGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt   <  1:638217/150‑1 (MQ=255)
                                                                                                                                            aaGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt   >  3:1260896/1‑150 (MQ=255)
                                                                                                                                             aGTGACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTc  >  1:2032692/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTC  >  NC_000913_3_pae_pgi/2867116‑2867406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: