Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,159,282 G→T E472* (GAG→TAG)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,159,2820GT99.2% 445.2 / ‑3.7 124E472* (GAG→TAG) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (62/61);  total (63/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 124 total aligned reads displayed.

CCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACC  >  NC_000913_3_pae_pgi/1159136‑1159421
                                                                                                                                                  |                                                                                                                                           
cccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGt                                                                                                                                          <  4:58526/150‑1 (MQ=255)
    tCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACAt                                                                                                                                      >  1:283799/1‑150 (MQ=255)
     cTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATc                                                                                                                                     <  1:404993/150‑1 (MQ=255)
      tGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATcc                                                                                                                                    >  3:51744/1‑150 (MQ=255)
        cgcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGt                                                                                                                                  >  3:68984/1‑150 (MQ=255)
                cGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTa                                                                                                                          <  2:150581/150‑1 (MQ=255)
                  ttGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAAt                                                                                                                        <  2:24024/150‑1 (MQ=255)
                    gCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATcc                                                                                                                      >  1:1091602/1‑150 (MQ=255)
                     cTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCg                                                                                                                     <  2:631008/150‑1 (MQ=255)
                     cTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCg                                                                                                                     >  1:374503/1‑150 (MQ=255)
                      tGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGt                                                                                                                    >  4:775074/1‑150 (MQ=255)
                         tgtgTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAg                                                                                                                 >  4:379995/1‑150 (MQ=255)
                            gtCTAAAGTGGAACAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTATTCAGGCGATTCTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCAGTAACCACTAATCCGTAAGACg                                                                                                              >  4:133427/1‑150 (MQ=255)
                              cTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAAc                                                                                                                               >  1:1083081/1‑131 (MQ=255)
                              cTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAAc                                                                                                                               <  2:1083081/131‑1 (MQ=255)
                               tAAAGTGGATCAGGCCGGCCTGAAGGAACTGGGCTCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTg                                                                                                           >  4:320353/1‑150 (MQ=255)
                               tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTg                                                                                                           >  1:57275/1‑150 (MQ=255)
                               tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTg                                                                                                           >  1:807877/1‑150 (MQ=255)
                               tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTg                                                                                                           <  3:506549/150‑1 (MQ=255)
                                aaaGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAAt                                                                                                                        <  3:17904/136‑1 (MQ=255)
                                aaaGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAAt                                                                                                                        >  4:17904/1‑136 (MQ=255)
                                aaaGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTgg                                                                                                          >  3:143320/1‑150 (MQ=255)
                                aaaGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTgg                                                                                                          <  3:480405/150‑1 (MQ=255)
                                      gATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGAc                                                                                                    >  4:358078/1‑150 (MQ=255)
                                       aTCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGt                                                                                                                                  >  3:244778/1‑119 (MQ=255)
                                       aTCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGt                                                                                                                                  <  4:244778/119‑1 (MQ=255)
                                        tCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTa                                                                                                  >  3:51666/1‑150 (MQ=255)
                                         cAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTaa                                                                                                 <  1:865190/150‑1 (MQ=255)
                                         cAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTaa                                                                                                 <  3:253251/150‑1 (MQ=255)
                                           ggccggccTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAgg                                                                                               <  4:611604/150‑1 (MQ=255)
                                                  cTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAg                                                                                        >  1:779906/1‑150 (MQ=255)
                                                   tGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGa                                                                                       <  2:243941/150‑1 (MQ=255)
                                                    gaagaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAt                                                                                      >  4:52577/1‑150 (MQ=255)
                                                       gaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCatctggc                                                                                   >  3:806846/1‑144 (MQ=255)
                                                         aaCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTg                                                                                 <  2:1207967/150‑1 (MQ=255)
                                                          aCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACATGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGc                                                                                >  1:1014966/1‑150 (MQ=255)
                                                           cTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACAGCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGcc                                                                               <  3:696771/150‑1 (MQ=255)
                                                              ggCGCGGAGGGCGTAGTGGTTGCTGGTTGTGGTGTTCAGGCGATTTTCGGTACTATATCGGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCAtta                                                                            <  4:167875/150‑2 (MQ=255)
                                                              ggCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCCtta                                                                            <  2:430896/150‑2 (MQ=255)
                                                              ggCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCCtta                                                                            <  3:89339/150‑2 (MQ=255)
                                                                 gcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCtttttt                                                                         >  2:660232/1‑150 (MQ=255)
                                                                   aGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTgggg                                                                                                         <  4:645444/116‑1 (MQ=255)
                                                                   aGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTggg                                                                                                         >  3:645444/1‑116 (MQ=255)
                                                                      gggCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAgg                                                                    >  4:274857/1‑150 (MQ=255)
                                                                              tGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTc                                                            <  1:154918/150‑1 (MQ=255)
                                                                               ggTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCa                                                           <  2:807877/150‑1 (MQ=255)
                                                                                  tGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGaa                                                        <  2:759093/150‑1 (MQ=255)
                                                                                   gCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAAt                                                       <  3:775074/150‑1 (MQ=255)
                                                                                   gCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAAt                                                       <  3:802675/150‑1 (MQ=255)
                                                                                     tGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTg                                                     <  1:564709/150‑1 (MQ=255)
                                                                                      ggTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGa                                                    <  4:462514/150‑1 (MQ=255)
                                                                                       gTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg                                                                     >  3:705927/1‑132 (MQ=255)
                                                                                       gTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg                                                                     <  4:705927/132‑1 (MQ=255)
                                                                                       gTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGAt                                                   <  2:729754/150‑1 (MQ=255)
                                                                                        ttCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGc                                                                                           <  2:1192945/109‑1 (MQ=255)
                                                                                        ttCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGc                                                                                           >  1:1192945/1‑109 (MQ=255)
                                                                                         tCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGc                                                                                           >  2:941034/1‑108 (MQ=255)
                                                                                         tCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGc                                                                                           <  1:941034/108‑1 (MQ=255)
                                                                                          cTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACg                                                >  1:117010/1‑150 (MQ=255)
                                                                                              tgtTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGcc                                            <  2:328627/150‑1 (MQ=255)
                                                                                               gtTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCg                                           <  4:143320/150‑1 (MQ=255)
                                                                                                ttCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCgg                                          >  3:284692/1‑150 (MQ=255)
                                                                                                   aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCgg                                          >  2:923643/1‑147 (MQ=255)
                                                                                                       gATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGATGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCt                                   >  2:963472/1‑150 (MQ=255)
                                                                                                         ttttCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGa                                 <  2:1074332/150‑1 (MQ=255)
                                                                                                            tCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAAt                              >  1:29749/1‑150 (MQ=255)
                                                                                                              ggTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCttttt                                                                          >  3:450192/1‑104 (MQ=255)
                                                                                                               gTACTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAc                           >  1:222435/1‑150 (MQ=255)
                                                                                                                 aCTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgc                         <  1:714804/150‑1 (MQ=255)
                                                                                                                  cTAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgcg                        >  2:1097302/1‑150 (MQ=255)
                                                                                                                   tAAATCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTaa                                                                                                 >  2:270031/1‑76 (MQ=255)
                                                                                                                    aaaTCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGATTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgt                      <  2:991760/150‑1 (MQ=255)
                                                                                                                    aaaTCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgt                      <  3:691356/150‑1 (MQ=255)
                                                                                                                     aaTCCGATACCCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgtg                     >  1:907897/1‑150 (MQ=255)
                                                                                                                     aaTCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgtg                     <  4:284692/150‑1 (MQ=255)
                                                                                                                      aTCCGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGAc                                                                                                               <  2:151350/59‑1 (MQ=255)
                                                                                                                        ccGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGc                  <  3:379995/150‑1 (MQ=255)
                                                                                                                        ccGATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGc                  <  3:274857/150‑1 (MQ=255)
                                                                                                                         cGATAACCTGAAAACCGAGATGGATTAGTACATCAGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGcc                 <  3:133427/150‑1 (MQ=255)
                                                                                                                          gATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCatc                                                                                       >  2:597198/1‑77 (MQ=255)
                                                                                                                          gATAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCatc                                                                                       <  1:597198/79‑3 (MQ=255)
                                                                                                                           aTAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTggg                                                                                                         >  1:283830/1‑60 (MQ=255)
                                                                                                                           aTAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTggg                                                                                                         <  2:283830/60‑1 (MQ=255)
                                                                                                                           aTAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTggg                                                                                                         <  2:284205/60‑1 (MQ=255)
                                                                                                                           aTAACCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTggg                                                                                                         >  1:284205/1‑60 (MQ=255)
                                                                                                                              aCCTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGa            >  1:204720/1‑150 (MQ=255)
                                                                                                                               ccTGAAAACCGAGATGGTTTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc           <  4:460318/150‑1 (MQ=255)
                                                                                                                               ccTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc           <  4:349159/150‑1 (MQ=255)
                                                                                                                                cTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGgaga                                                                                                     >  3:441807/1‑59 (MQ=255)
                                                                                                                                cTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGgaga                                                                                                     <  4:441807/59‑1 (MQ=255)
                                                                                                                                cTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACg          >  2:1084532/1‑150 (MQ=255)
                                                                                                                                cTGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACg          >  1:47987/1‑150 (MQ=255)
                                                                                                                                 tGAAAACCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGa         <  1:2548/150‑1 (MQ=255)
                                                                                                                                   aaaaCCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTGCGCGGTGTGCCGTAGACGATa       <  3:196466/150‑1 (MQ=255)
                                                                                                                                   aaaaCCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATa       <  2:283799/150‑1 (MQ=255)
                                                                                                                                   aaaaCCGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATa       >  1:370604/1‑150 (MQ=255)
                                                                                                                                     aaCCGAGATGGATTAGTACATCCGTAACCACTAATCCGTaa                                                                                                                  >  2:952505/1‑41 (MQ=255)
                                                                                                                                     aaCCGAGATGGATTAGTACATCCGTAACCACTAATCCGTaa                                                                                                                  <  1:952505/41‑1 (MQ=255)
                                                                                                                                       ccGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGCTAATGCTGAAATTACGCGGAGTGCCGTCCACGATAGAAc   >  2:1097346/1‑150 (MQ=255)
                                                                                                                                        cGAGATGGATTAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAcc  >  3:393789/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
CCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACC  >  NC_000913_3_pae_pgi/1159136‑1159421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: