Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,440,243 T→G T263P (ACT→CCT)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,440,2430TG100.0% 317.5 / NA 90T263P (ACT→CCT) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (41/49);  total (41/49)

TCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAAT  >  NC_000913_3_pae_pgi/3440097‑3440381
                                                                                                                                                  |                                                                                                                                          
tCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTTTTTTTTTTAGACGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATTGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCac                                                                                                                                         >  2:21018/1‑149 (MQ=255)
   aCGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTTTTTTTTTTTTTCGTCTTAAGGAGCTCAACCCCGGTACCCTGTATGAGATCACCGCTATAGTGGATATCTTCTCCTTTCAGGCAGTTAGCAGAGCGGCCATGCAATTc                                                                                                                                      >  2:1122242/1‑150 (MQ=255)
     gggAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCa                                                                                                                                    <  2:152612/150‑1 (MQ=255)
        aaGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATc                                                                                                                                <  1:599312/150‑1 (MQ=255)
           ccAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGt                                                                                                                              <  1:97596/150‑1 (MQ=255)
             aGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCa                                                                                                                            >  4:638020/1‑150 (MQ=255)
             aGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCa                                                                                                                            >  2:1057703/1‑150 (MQ=255)
              gCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCaa                                                                                                                           <  3:460705/150‑1 (MQ=255)
                   tCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAACTCCAGATCGTCAACAggg                                                                                                                      >  3:275428/1‑150 (MQ=255)
                      ttAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGTTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGgcgc                                                                                                                   <  1:267373/150‑1 (MQ=255)
                          tctcAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCgcagca                                                                                                               <  3:671574/150‑1 (MQ=255)
                          tctcAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCgcagca                                                                                                               >  2:267873/1‑150 (MQ=255)
                              aGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggat                                                                                                           <  1:703024/150‑1 (MQ=255)
                               gTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatc                                                                                                          <  3:570022/150‑1 (MQ=255)
                               gTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatc                                                                                                          <  2:977780/150‑1 (MQ=255)
                                 aaGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatcgg                                                                                                        <  4:93853/150‑1 (MQ=255)
                                  agagATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatcgga                                                                                                       <  2:995119/150‑1 (MQ=255)
                                          tttACCAAGGTTAGGCGGTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAActc                                                                                               >  4:199090/1‑150 (MQ=255)
                                           ttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAActct                                                                                              <  2:428910/150‑1 (MQ=255)
                                              ccAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCCTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGt                                                                                           <  3:243266/150‑1 (MQ=255)
                                               cAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGtt                                                                                          >  4:102539/1‑150 (MQ=255)
                                               cAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGtt                                                                                          >  4:102728/1‑150 (MQ=255)
                                                  ggTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtct                                                                                       <  3:713917/150‑1 (MQ=255)
                                                  ggTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtct                                                                                       <  1:7892/150‑1 (MQ=255)
                                                  ggTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtct                                                                                       >  1:534601/1‑150 (MQ=255)
                                                        gCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTcttctt                                                                                 >  4:604987/1‑150 (MQ=255)
                                                         cGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCttt                                                                                <  2:565460/150‑1 (MQ=255)
                                                               aaGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTc                                                                          <  3:459905/150‑1 (MQ=255)
                                                                aGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCa                                                                         <  2:1063502/150‑1 (MQ=255)
                                                                 ggAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAg                                                                        >  1:1089116/1‑150 (MQ=255)
                                                                  gAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAgg                                                                       >  2:115085/1‑150 (MQ=255)
                                                                  gAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAgg                                                                       >  2:913598/1‑150 (MQ=255)
                                                                     cTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTg                                                                    >  3:503568/1‑150 (MQ=255)
                                                                     cTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTg                                                                    >  4:703954/1‑150 (MQ=255)
                                                                      tCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGa                                                                   <  1:267873/150‑1 (MQ=255)
                                                                        aaCCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACg                                                                 <  2:343637/150‑1 (MQ=255)
                                                                         aCCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGt                                                                <  1:537105/150‑1 (MQ=255)
                                                                          ccccGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTa                                                               >  2:394151/4‑150 (MQ=255)
                                                                           cccGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCAc                                                                             <  4:111165/133‑1 (MQ=255)
                                                                           cccGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCAc                                                                             >  3:111165/3‑135 (MQ=255)
                                                                           cccGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTAc                                                              <  1:297478/148‑1 (MQ=255)
                                                                           cccGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTAc                                                              <  2:133557/148‑1 (MQ=255)
                                                                                tACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCat                                                         <  4:584192/150‑2 (MQ=255)
                                                                                   gCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAgga                                                                                                            >  3:710154/1‑96 (MQ=255)
                                                                                   gCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAgga                                                                                                            <  4:710154/96‑1 (MQ=255)
                                                                                   gCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGACCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTa                                                      >  4:518403/1‑150 (MQ=255)
                                                                                    cTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTaa                                                     <  1:1057703/150‑1 (MQ=255)
                                                                                    cTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTaa                                                     >  2:396849/1‑150 (MQ=255)
                                                                                    cTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTaa                                                     >  1:405201/1‑150 (MQ=255)
                                                                                          cAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAAc                                               <  2:405201/150‑1 (MQ=255)
                                                                                                aCCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCaa                                                <  1:825576/143‑1 (MQ=255)
                                                                                                aCCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCaa                                                >  2:825576/1‑143 (MQ=255)
                                                                                                  cGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatcggatc                                                                                                     >  3:266342/1‑88 (MQ=255)
                                                                                                  cGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatcggatc                                                                                                     <  4:266342/88‑1 (MQ=255)
                                                                                                  cGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCtt                                       >  2:430579/1‑150 (MQ=255)
                                                                                                   gATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTc                                      <  1:662149/150‑1 (MQ=255)
                                                                                                   gATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTc                                      <  2:1089116/150‑1 (MQ=255)
                                                                                                    atatAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTcc                                     <  1:820243/150‑1 (MQ=255)
                                                                                                     tataGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCa                                    <  1:913598/150‑1 (MQ=255)
                                                                                                        aGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGGCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgtt                                 >  3:103231/1‑150 (MQ=255)
                                                                                                         gTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttg                                >  1:624913/1‑150 (MQ=255)
                                                                                                         gTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttg                                >  2:276902/1‑150 (MQ=255)
                                                                                                           ggATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttgtt                              <  2:534601/150‑1 (MQ=255)
                                                                                                            gATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatcgga                                                                                                       >  2:845480/1‑76 (MQ=255)
                                                                                                            gATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatcgga                                                                                                       <  1:845480/76‑1 (MQ=255)
                                                                                                            gATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTc                             <  4:272162/150‑1 (MQ=255)
                                                                                                            gATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTc                             <  4:272541/150‑1 (MQ=255)
                                                                                                             aTAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTAc                                                              <  2:69630/116‑1 (MQ=255)
                                                                                                             aTAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTAc                                                              >  1:69630/1‑116 (MQ=255)
                                                                                                             aTAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCa                            <  3:199090/150‑1 (MQ=255)
                                                                                                             aTAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCa                            >  1:217144/1‑150 (MQ=255)
                                                                                                               aGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGc                          <  3:638020/150‑1 (MQ=255)
                                                                                                                 cTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCa                        >  1:454099/1‑150 (MQ=255)
                                                                                                                   tCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGa                      >  2:267345/1‑150 (MQ=255)
                                                                                                                       cTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGaa                                                                                                  <  2:279489/70‑1 (MQ=255)
                                                                                                                       cTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGaa                                                                                                  >  1:279489/1‑70 (MQ=255)
                                                                                                                        tttAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTaa                                                     >  1:1027548/1‑114 (MQ=255)
                                                                                                                        tttAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTaa                                                     <  2:1027548/114‑1 (MQ=255)
                                                                                                                         ttAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGtt                <  2:396168/150‑1 (MQ=255)
                                                                                                                          tAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTg               <  3:539441/150‑1 (MQ=255)
                                                                                                                            aGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGcc             >  2:540226/1‑150 (MQ=255)
                                                                                                                              gCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGc           >  1:501007/1‑150 (MQ=255)
                                                                                                                               cAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCGGTTGTTCAGCCAGAAt                    <  2:597073/140‑1 (MQ=255)
                                                                                                                               cAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAAt                    >  1:597073/1‑140 (MQ=255)
                                                                                                                                aGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTGCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCac         <  1:512358/150‑1 (MQ=255)
                                                                                                                                  ttAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCacga       >  1:752991/1‑150 (MQ=255)
                                                                                                                                  ttAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCacga       <  1:267345/150‑1 (MQ=255)
                                                                                                                                   tAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGgcgc                                                                                                                   >  2:1073304/1‑41 (MQ=255)
                                                                                                                                   tAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGgcgc                                                                                                                   <  1:1073304/41‑1 (MQ=255)
                                                                                                                                       agagCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAAt  >  4:421006/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                          
TCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAAT  >  NC_000913_3_pae_pgi/3440097‑3440381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: