Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,486,599 C→A H160Q (CAC→CAA)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,486,5990CA96.2% 359.2 / 2.5 105H160Q (CAC→CAA) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (44/56);  total (46/59)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCAC  >  NC_000913_3_pae_pgi/3486452‑3486745
                                                                                                                                                   |                                                                                                                                                  
ggACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAacc                                                                                                                                                  <  1:197725/150‑1 (MQ=255)
  aCATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTAACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGCCGCTATGACTCAACCgg                                                                                                                                                >  4:40754/1‑150 (MQ=255)
   cATTCTGATGCGTTTGCCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGa                                                                                                                                               <  2:822480/150‑1 (MQ=255)
     ttCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACg                                                                                                                                             <  2:387050/150‑1 (MQ=255)
        tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTa                                                                                                                                          >  2:932633/1‑150 (MQ=255)
           tGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAGCCGGACgggtaat                                                                                                                                       >  2:1071981/1‑145 (MQ=255)
           tGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGc                                                                                                                                       <  3:712484/150‑1 (MQ=255)
           tGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGc                                                                                                                                       <  2:309257/150‑1 (MQ=255)
                  gTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCaa                                                                                                                                >  1:153661/1‑150 (MQ=255)
                   tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCaaa                                                                                                                               >  1:223396/1‑150 (MQ=255)
                   tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCGGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCaaa                                                                                                                               <  1:715382/150‑1 (MQ=255)
                    cggCACAGATGGCGCGGCGTCGGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCaaaa                                                                                                                              <  4:537659/148‑1 (MQ=255)
                     tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAAt                                                                                                                             >  4:82004/1‑150 (MQ=255)
                        acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAc                                                                                                                          <  1:341820/150‑1 (MQ=255)
                        acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAc                                                                                                                          >  3:68578/1‑150 (MQ=255)
                         caGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAcc                                                                                                                         <  2:1195789/150‑1 (MQ=255)
                         caGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAcc                                                                                                                         <  3:490867/150‑1 (MQ=255)
                          aGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAccc                                                                                                                        >  2:1041695/1‑150 (MQ=255)
                              ggCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCa                                                                                                                    <  1:785151/150‑1 (MQ=255)
                                 gcgTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGa                                                                                                                 >  4:346798/1‑150 (MQ=255)
                                    tcgtcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCGACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAAt                                                                                                              <  1:1070735/150‑1 (MQ=255)
                                       tcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTgg                                                                                                           >  4:188244/1‑150 (MQ=255)
                                           cAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAg                                                                                                       >  3:89162/1‑150 (MQ=255)
                                           cAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAg                                                                                                       >  3:95571/1‑150 (MQ=255)
                                            aaGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGa                                                                                                      <  1:339251/150‑1 (MQ=255)
                                               tCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTg                                                                                                   >  2:917718/1‑150 (MQ=255)
                                                   ttCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                               >  3:569875/1‑150 (MQ=255)
                                                   ttCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                               >  1:183006/1‑150 (MQ=255)
                                                   tgcAGAGAGAGTGGGCAACCTGGCGTTGCTCGATGTGAGGGGGCGGATTGCACGGACTCTGCTGACTTTGGCAAAAGAACCAAAGGCTGTGACTCAACCAGGCGGTGTGCTAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                               <  2:876338/148‑1 (MQ=255)
                                                    tCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTTGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGc                                                                                              >  1:512601/1‑150 (MQ=255)
                                                      agagAAAGTGGGCAACCTGGCGTTCCTCGACGTTACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATTCAAATCAAAATTACCCGGCAGGAAATTGTGTAGATTGTCGGCTg                                                                                            >  4:312882/1‑150 (MQ=255)
                                                            aGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCg                                                                                      >  1:341887/1‑150 (MQ=255)
                                                             gTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGt                                                                                     <  2:1067979/150‑1 (MQ=255)
                                                             gTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGt                                                                                     <  2:1156614/150‑1 (MQ=255)
                                                               gggCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGa                                                                                   <  3:188244/150‑1 (MQ=255)
                                                               ggccAACCTGGCGTGCCTCGACGTGACGGGCCGCATCGCACAGACTCTGCTGAATTCGGAAAAACAAACAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATAGGTCAGATTGTCGGCTTTGCTCgaga                                                                                   <  4:573181/147‑1 (MQ=255)
                                                                  cAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAAc                                                                                >  1:737009/1‑150 (MQ=255)
                                                                   aaCCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAAcc                                                                               >  2:288399/1‑150 (MQ=255)
                                                                     ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCaaa                                                                                                                               >  1:108908/1‑100 (MQ=255)
                                                                     ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCaaa                                                                                                                               <  2:108908/100‑1 (MQ=255)
                                                                     ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGt                                                                             >  4:203189/1‑150 (MQ=255)
                                                                     ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGt                                                                             <  2:1050478/150‑1 (MQ=255)
                                                                      cTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTg                                                                            <  4:569875/150‑1 (MQ=255)
                                                                       tGGCGTTCCTCGCAGGGACGGGCAGCATGGCACAGATTCTGATGAATCTGGCAAAACAAACAGATGTTATGATTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTgg                                                                           <  2:304740/150‑1 (MQ=255)
                                                                       tGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTgg                                                                           <  1:932633/150‑1 (MQ=255)
                                                                        ggCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTggg                                                                          <  4:558625/150‑1 (MQ=255)
                                                                            ttCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGc                                                                      <  4:682412/150‑1 (MQ=255)
                                                                                tCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTc                                                                  >  3:182080/1‑150 (MQ=255)
                                                                                   aCGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGa                                                               <  3:410968/150‑1 (MQ=255)
                                                                                   aCGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGa                                                               <  4:562736/150‑1 (MQ=255)
                                                                                    cGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                                                              <  1:958084/150‑1 (MQ=255)
                                                                                    cGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                                                              <  2:341887/150‑1 (MQ=255)
                                                                                    cGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGGTCTCGTGAAACCGTGGGACGCATTCTGaa                                                              <  3:82004/150‑1 (MQ=255)
                                                                                       gACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTa                                                                                                                           >  3:592829/1‑86 (MQ=255)
                                                                                       gACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTa                                                                                                                           <  4:592829/86‑1 (MQ=255)
                                                                                       gACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGAt                                                           >  2:522988/1‑150 (MQ=255)
                                                                                           ggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                                                       <  1:998139/150‑1 (MQ=255)
                                                                                             ccGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGt                                                                                     >  4:300483/1‑118 (MQ=255)
                                                                                             ccGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGt                                                                                     <  3:300483/118‑1 (MQ=255)
                                                                                             ccGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGa                                                     <  1:1041695/150‑1 (MQ=255)
                                                                                                cATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGAGGCTATGACTCAACCGGACGTTATGCAAATCAAAATTACCCGTCAGGAAATGGGTCAGATTGTCGGCTGTTCTCGTCAACCCGTGGGACGCATTCTGAAGATCCTAGAAgc                                                  >  4:201021/1‑149 (MQ=255)
                                                                                                 aTTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAACTCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGAt                                                 <  4:14673/150‑1 (MQ=255)
                                                                                                 aTTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGAt                                                 <  1:346346/150‑1 (MQ=255)
                                                                                                      acaGACTCTGCTGAATCTGGCAAAACGACGAGACGCTATGACTCAACCGGAGGGTATGCAAATCAAGATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGaa                                            <  4:619717/150‑1 (MQ=255)
                                                                                                      acaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGACGATCAGaa                                            >  2:799726/1‑150 (MQ=255)
                                                                                                      acaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGaa                                            >  1:498016/1‑150 (MQ=255)
                                                                                                      acaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGaa                                            >  2:737353/1‑150 (MQ=255)
                                                                                                       caGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAc                                           >  2:847140/1‑150 (MQ=255)
                                                                                                       caGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAc                                           <  1:520937/150‑1 (MQ=255)
                                                                                                       caGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAc                                           <  3:598864/150‑1 (MQ=255)
                                                                                                         gACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCt                                         >  2:32642/1‑150 (MQ=255)
                                                                                                         gACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTACGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGAGGCTGGAAGATCAGAACCt                                         <  2:756408/150‑1 (MQ=255)
                                                                                                            tctGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAc                                           <  1:487213/145‑1 (MQ=255)
                                                                                                            tctGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAt                                      >  1:334919/1‑150 (MQ=255)
                                                                                                            tctGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAt                                      >  3:357949/1‑150 (MQ=255)
                                                                                                                ctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGa                                                                                   <  4:271344/101‑1 (MQ=255)
                                                                                                                ctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGa                                                                                   >  3:271344/1‑101 (MQ=255)
                                                                                                                 tgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTg                                                                                    <  1:62859/99‑1 (MQ=255)
                                                                                                                 tgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTg                                                                                    >  2:62859/1‑99 (MQ=255)
                                                                                                                        ggCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACgg                          <  4:68578/150‑1 (MQ=255)
                                                                                                                         gCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGc                                >  1:797424/1‑143 (MQ=255)
                                                                                                                         gCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGc                                <  2:797424/143‑1 (MQ=255)
                                                                                                                          cAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTa                        >  1:898706/1‑150 (MQ=255)
                                                                                                                           aaaaCAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCACAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGCGACGCAGTCTGAAGATGCTGGAAGATCTGAACCTGATCTCCGCACACGGTaa                       >  3:593186/1‑150 (MQ=255)
                                                                                                                           aaaaCAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTaa                       >  1:1141825/1‑150 (MQ=255)
                                                                                                                           aaaaCAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTaa                       <  1:743522/150‑1 (MQ=255)
                                                                                                                           aaaaCAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTaa                       >  1:800131/1‑150 (MQ=255)
                                                                                                                           aaaaCAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTaa                       <  3:40754/150‑1 (MQ=255)
                                                                                                                           aaaaCAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTaa                       <  1:426483/150‑1 (MQ=255)
                                                                                                                           aaaaCAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTaa                       <  1:426500/150‑1 (MQ=255)
                                                                                                                               caacCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAAcc                   <  3:346798/150‑1 (MQ=255)
                                                                                                                                 acCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAt                 <  1:847140/150‑1 (MQ=255)
                                                                                                                                   cAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtcg               >  1:912675/1‑150 (MQ=255)
                                                                                                                                     gACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtcgtc             <  1:288399/150‑1 (MQ=255)
                                                                                                                                      aCGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtcgtcg            <  4:428765/150‑1 (MQ=255)
                                                                                                                                          tATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGtttc        >  3:552274/1‑149 (MQ=255)
                                                                                                                                            tGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACg      <  2:1118459/150‑1 (MQ=255)
                                                                                                                                            tGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACg      <  2:153661/150‑1 (MQ=255)
                                                                                                                                             gACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACgg     <  2:223396/150‑1 (MQ=255)
                                                                                                                                                tCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCAc  >  1:1048895/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCAC  >  NC_000913_3_pae_pgi/3486452‑3486745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑