New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi = 12994980 (0.000)54 (0.900) 42/272 0.5 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_pgi/1299350‑1299498
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_pgi/1300698‑1300843
                                                                                                                                                                                                                                                                                                         
CTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAA                                                                                                                                                    <  1:106760/149‑1
      AAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCATCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATA                                                                                                                                              <  2:486881/149‑1
      AAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTATACTCACCATAATGTTATTTTCGCCACATCATAATCCTGGACTTGATGAAGAATAATTGAAATGATATTATTAATTCCACTGCATTTA‑‑GTAAAGGAAAGTGGTAACTAATA                                                                                                                                              >  2:381169/1‑149
        ATTACATTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACTTCGTAATCCTGGGCTTGCTGAATAATAATTGAAATTATACTATTAATTCCACTGCATTTG‑‑GTAGAGGACACTGCTAAATAATAAT                                                                                                                                            <  2:975026/149‑1
                   TTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAA                                                                                                                                 >  1:856225/1‑149
                   TTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAA                                                                                                                                 <  1:349301/149‑1
                      TAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGCAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGATAGTGCTAAATAATAATCACTTGTTTAATTA                                                                                                                              >  2:439202/1‑149
                      TAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATCATCAATTGTTAATTTA                                                                                                                              >  2:985455/1‑149
                      TAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTA                                                                                                                              >  2:166477/1‑149
                         GGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGACATATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTG                                                                                                                           <  2:389682/149‑1
                             TTCCTTAATTGCTTCTCCTCCCCATCATGTTATTTTCGCAACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTTCACTGCCTTTG‑‑GTTGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCA                                                                                                                       <  2:455098/149‑1
                                CTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTACTACGACTTCCATCGCAGTAGTGG‑‑TGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTT                                                                                                                    <  1:1060891/149‑1
                                    ACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACT                                                                                                                >  2:105105/1‑149
                                            TCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAC                                                                                                        >  1:774980/1‑149
                                            TCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTAATGGAAC                                                                                                        >  1:682604/1‑149
                                                 ACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAA                                                                                                   <  1:394579/149‑1
                                                   CATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATC                                                                                                 >  2:1069779/1‑149
                                                         GTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAA                                                                                                                                         >  1:613913/1‑103
                                                         GTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAA                                                                                                                                         <  2:613913/103‑1
                                                                      CATCATAATCCTGGGCTTGCTGAAGAATAATTGAGATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTT                                                                              >  2:701952/1‑149
                                                                              TCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATC                                                                      <  1:358409/149‑1
                                                                                CTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTC                                                                    >  2:895595/1‑149
                                                                                         CTGAAGAATAATTGAAACGATAGTCTTCATTCCACTGCCTTTG‑‑GTTGAGGAAAGTGCTAAATAATAATCACTCGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTCAGCCAATCTCTATTTGATT                                                           <  2:911442/149‑1
                                                                                           GAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGATGAAAGTGCGAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGA                                                         >  2:259839/1‑149
                                                                                             gGAATAAGTGAAAGGATATACATAATTTAACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATGATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAAT                                                       <  2:174410/148‑1
                                                                                              GAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGGTAAATAATAATCAATTGTTAAATTATTGTGTATTTAACTATTGGAAATGTGATCAGGAAAGATAGAAATGCTTAGCTAATTTCTAGTTGGTTTAATT                                                      >  2:1069463/1‑149
                                                                                              GAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAATTAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATT                                                      >  1:1016106/1‑149
                                                                                              GAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGAATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGACAATCTCTATTTGATTGCATT                                                      >  1:1028142/1‑149
                                                                                                       AAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATT                                                                                                                            <  1:576649/70‑1
                                                                                                       AAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATT                                                                                                                            >  2:576649/1‑70
                                                                                                         ATGAGATTATTAATTCCACTGCCTTTG‑‑GGAGAGGAAAGTGGTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTT                                           <  2:524559/149‑1
                                                                                                            ATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTT                                        <  1:105105/149‑1
                                                                                                              ATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAA                                      <  1:439202/149‑1
                                                                                                               TTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTATTCTGAAAAGATAGAAATGCTTAGCCAATCTCTATTTGATTGAATTGAACGATGTTTGTTAAG                                     >  2:555706/1‑149
                                                                                                               TTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCA                                                                                                                       >  2:931060/1‑67
                                                                                                               TTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGCTAAATTATTGTGCA                                                                                                                       <  1:931060/67‑1
                                                                                                                TATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATCGAAAGATGTTTGTTAAGG                                    >  2:735611/1‑149
                                                                                                                  TTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATA                                                                                       >  2:21161/1‑96
                                                                                                                  TTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATA                                                                                       <  1:21161/96‑1
                                                                                                                       TCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAT                             <  1:701952/149‑1
                                                                                                                       TCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAT                             <  1:166477/149‑1
                                                                                                                          ACTGCCTTTG‑‑GTAGAGGAAAGTGCTGAATAATAATCAATTGTTAAAATATTGTGCATTTCACTACTATAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGATAGATGTTTGTTAAGGCATGGATGCA                          >  1:975800/1‑149
                                                                                                                            TGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAG                        >  1:207924/1‑149
                                                                                                                            TGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAG                        >  2:289806/1‑149
                                                                                                                            TGCCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAG                        <  2:207924/149‑1
                                                                                                                              CCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGC                                                                           >  2:424018/1‑96
                                                                                                                              CCTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGC                                                                           <  1:424018/96‑1
                                                                                                                               gTTTG‑‑GTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTA                     <  2:517264/148‑1
                                                                                                                                         GAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGCACTCGGGCGAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCT             <  1:1056746/149‑1
                                                                                                                                         GAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCT             <  1:895595/149‑1
                                                                                                                                          AGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTG            >  2:582537/1‑149
                                                                                                                                            GAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGAT          >  1:478447/1‑149
                                                                                                                                                 gGCTAAAGAATAATCAAGTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAGGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT     <  2:81079/148‑1
                                                                                                                                                 TGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT     <  2:856225/149‑1
                                                                                                                                                 TGCTAAATAAGAACAAATTGTTCAATTATTGGGCATTTCACTCCTGGAACGGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT     <  1:175529/149‑1
                                                                                                                                                    TAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  1:804264/1‑149
                                                                                                                                                                                                                                                                                                         
CTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTG‑‑GTAGAGGAAAGTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_pgi/1299350‑1299498
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_pgi/1300698‑1300843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.