Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 4,234,667 Δ1 bp intergenic (‑1434/‑499) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,234,6660A.97.2% 155.0 / ‑3.4 36intergenic (‑1433/‑500)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (18/17);  total (18/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATA  >  NC_000913_3_pae_pgi/4234527‑4234801
                                                                                                                                           |                                                                                                                                       
aCGCAGTAGAGGTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCAtcgtcg                                                                                                                               <  1:279948/149‑1 (MQ=255)
aCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCAtcgtcg                                                                                                                               >  1:10583/1‑149 (MQ=255)
aCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAAGGATTAGAGGCGTAAATCCCCTGGCCCCCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCAtcgccg                                                                                                                               >  1:315101/1‑149 (MQ=255)
         agaTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAgg                                                                                                                      >  1:56114/1‑149 (MQ=255)
                    aaGGGCGTTTTATCCTTTTTGCGAGATGTGGGCATCGCCTGTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCTCTTGCCACCTCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                           <  2:229904/149‑1 (MQ=255)
                       ggCGTTTTATCTTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                        <  1:83013/149‑1 (MQ=255)
                       ggCGTTGTATCCTTTTTACGAGATGGGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                        <  1:222989/149‑1 (MQ=255)
                           ttttATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcg                                                                                                    >  1:259705/1‑149 (MQ=255)
                              tATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgccg                                                                                                 >  1:425980/1‑149 (MQ=255)
                                 ttttttttCGACATGTGTGCATCGCCTCTGTAAAACTGGCAGAAATTATAGTCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                              <  2:358728/147‑1 (MQ=255)
                                   tttttACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGACGAGGCGCCCTCTGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                            >  1:211453/1‑149 (MQ=255)
                                   tttttACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCGGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                            >  1:332710/1‑149 (MQ=255)
                                   tttttACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                            >  2:77901/1‑149 (MQ=255)
                                   tttttACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                            >  2:255293/1‑149 (MQ=255)
                                          gagaTGTGTGCATCGCCTGTGTAAAACTGGCAGAGATTAGAGGCTTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                                                                     <  2:32820/149‑1 (MQ=255)
                                          gagaTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                                                                     >  1:408176/1‑149 (MQ=255)
                                               gtgGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                <  2:335282/149‑1 (MQ=255)
                                                  ggCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTg                                                                             >  1:448244/1‑149 (MQ=255)
                                                   gCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                            >  2:423579/1‑149 (MQ=255)
                                                   gCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCACCTGGCCACCGCGAGAATAGTTGTTTTGAGGAGGCGTATG‑TCAGAAGTGTGGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                            <  1:112580/149‑1 (MQ=25)
                                                     aTCGACTCTGTAAAACTGGCAGAGATTAGAAGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATCGAAGAGGCGCTTGCAGCG‑CTCGTCGTCGCTCTGTAGGCCGCAAAAGGCGATCACGCCGCATCCCTCAACCTATGccatatg                                                                          >  2:421923/1‑144 (MQ=255)
                                                                   aCTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                            <  1:282656/149‑1 (MQ=255)
                                                                         agagATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                      <  2:448244/149‑1 (MQ=255)
                                                                                    gCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTAGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACAgg                                           >  1:243893/1‑149 (MQ=255)
                                                                                     cGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                          <  1:77901/149‑1 (MQ=255)
                                                                                      gTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                      <  1:73458/120‑1 (MQ=255)
                                                                                      gTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                      >  2:73458/1‑120 (MQ=255)
                                                                                      gTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                         <  2:10583/149‑1 (MQ=255)
                                                                                            ccgcTGGGCAGCCCGAGAATAGGTGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGACGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                   <  2:79350/146‑1 (MQ=25)
                                                                                                 ggCCACCGCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                              <  2:211453/149‑1 (MQ=255)
                                                                                                     aCCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAg                                                    >  2:150504/1‑123 (MQ=37)
                                                                                                        ccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                       >  1:427980/1‑149 (MQ=255)
                                                                                                                  aTGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCa             >  1:84049/1‑149 (MQ=255)
                                                                                                                        tGAAGAGGCGCTTGCAGTG‑ACCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt       <  1:394023/149‑1 (MQ=255)
                                                                                                                         gAAGGGGCGGTGGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa      <  2:408176/149‑1 (MQ=255)
                                                                                                                             agGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATa  <  2:427980/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
ACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATA  >  NC_000913_3_pae_pgi/4234527‑4234801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑